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Protein

Aminopeptidase YpdE

Gene

ypdE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a broad aminopeptidase activity on non-blocked peptides by progressively cleaving amino acids off the peptide substrate. Aminopeptidase activity stops at the residue before the first proline in the peptide. Cannot cleave when proline is the first N-terminal residue.1 Publication

Cofactori

Co2+Curated1 Publication, Ni2+Curated1 Publication, Mn2+Curated1 Publication, Cu2+Curated1 PublicationNote: Binds 2 divalent metal cations per subunit. Can use cobalt, and to a lesser extent, nickel, manganese or copper.Curated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Divalent metal cation 1By similarity1
Metal bindingi166Divalent metal cation 1By similarity1
Metal bindingi166Divalent metal cation 2By similarity1
Active sitei198Proton acceptorBy similarity1
Metal bindingi199Divalent metal cation 2By similarity1
Metal bindingi221Divalent metal cation 1By similarity1
Metal bindingi308Divalent metal cation 2By similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: EcoliWiki
  • metal ion binding Source: UniProtKB-KW
  • metallopeptidase activity Source: EcoliWiki

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandCobalt, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7247-MONOMER
MetaCyc:G7247-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase YpdE (EC:3.4.11.-)
Gene namesi
Name:ypdE
Ordered Locus Names:b2384, JW2381
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14152 ypdE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000716591 – 345Aminopeptidase YpdEAdd BLAST345

Proteomic databases

PaxDbiP77585
PRIDEiP77585

Interactioni

Protein-protein interaction databases

BioGridi4260561, 10 interactors
DIPiDIP-48263N
IntActiP77585, 8 interactors
STRINGi316385.ECDH10B_2549

Structurei

3D structure databases

ProteinModelPortaliP77585
SMRiP77585
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M42 family.Curated

Phylogenomic databases

eggNOGiENOG4105CN9 Bacteria
COG1363 LUCA
HOGENOMiHOG000291973
InParanoidiP77585
OMAiNTHGYEI
PhylomeDBiP77585

Family and domain databases

Gene3Di2.40.30.40, 1 hit
InterProiView protein in InterPro
IPR008007 Peptidase_M42
IPR023367 Peptidase_M42_dom2
PfamiView protein in Pfam
PF05343 Peptidase_M42, 1 hit
PIRSFiPIRSF001123 PepA_GA, 1 hit

Sequencei

Sequence statusi: Complete.

P77585-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLSLLKALS EADAIASSEQ EVRQILLEEA DRLQKEVRFD GLGSVLIRLN
60 70 80 90 100
ESTGPKVMIC AHMDEVGFMV RSISREGAID VLPVGNVRMA ARQLQPVRIT
110 120 130 140 150
TREECKIPGL LDGDRQGNDV SAMRVDIGAR SYDEVMQAGI RPGDRVTFDT
160 170 180 190 200
TFQVLPHQRV MGKAFDDRLG CYLLVTLLRE LHDAELPAEV WLVASSSEEV
210 220 230 240 250
GLRGGQTATR AVSPDVAIVL DTACWAKNFD YGAANHRQIG NGPMLVLSDK
260 270 280 290 300
SLIAPPKLTA WVETVAAEIG VPLQADMFSN GGTDGGAVHL TGTGVPTVVM
310 320 330 340
GPATRHGHCA ASIADCRDIL QMQQLLSALI QRLTRETVVQ LTDFR
Length:345
Mass (Da):37,409
Last modified:February 1, 1997 - v1
Checksum:iFB2D88A5C11590A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75443.1
AP009048 Genomic DNA Translation: BAA16254.1
PIRiE65012
RefSeqiNP_416885.1, NC_000913.3
WP_000366028.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75443; AAC75443; b2384
BAA16254; BAA16254; BAA16254
GeneIDi946848
KEGGiecj:JW2381
eco:b2384
PATRICifig|1411691.4.peg.4344

Similar proteinsi

Entry informationi

Entry nameiYPDE_ECOLI
AccessioniPrimary (citable) accession number: P77585
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health