P77581 (ASTC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinylornithine transaminase
Short name=SOAT
EC=2.6.1.81

Alternative name(s):
Carbon starvation protein C
Succinylornithine aminotransferase

Gene names

Name:	astC
Synonyms:	argM, cstC, ydjW
Ordered Locus Names:	b1748, JW1737

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transamination of N(2)-succinylornithine and alpha-ketoglutarate into N(2)-succinylglutamate semialdehyde and glutamate. Can also act as an acetylornithine aminotransferase. Ref.5
Catalytic activity	N(2)-succinyl-L-ornithine + 2-oxoglutarate = N-succinyl-L-glutamate 5-semialdehyde + L-glutamate. HAMAP MF_01173
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 3/5. HAMAP MF_01173
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. AstC subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	arginine catabolic process Inferred from mutant phenotype Ref.5. Source: EcoliWiki ornithine catabolic process Inferred from mutant phenotype Ref.5. Source: EcoliWiki
Molecular function	pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro succinylornithine transaminase activity Inferred from direct assay Ref.5. Source: EcoliWiki
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-1125336,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Succinylornithine transaminase HAMAP MF_01173		PRO_0000120352
Amino acid modifications
	Modified residue	252	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	361	1	A → R in AAB51148. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P77581 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B18ED13644C21176	FASTA	406	43,665
	10 20 30 40 50 60 MSQPITRENF DEWMIPVYAP APFIPVRGEG SRLWDQQGKE YIDFAGGIAV NALGHAHPEL 70 80 90 100 110 120 REALNEQASK FWHTGNGYTN EPVLRLAKKL IDATFADRVF FCNSGAEANE AALKLARKFA 130 140 150 160 170 180 HDRYGSHKSG IVAFKNAFHG RTLFTVSAGG QPAYSQDFAP LPADIRHAAY NDINSASALI 190 200 210 220 230 240 DDSTCAVIVE PIQGEGGVVP ASNAFLQGLR ELCNRHNALL IFDEVQTGVG RTGELYAYMH 250 260 270 280 290 300 YGVTPDLLTT AKALGGGFPV GALLATEECA RVMTVGTHGT TYGGNPLASA VAGKVLELIN 310 320 330 340 350 360 TPEMLNGVKQ RHDWFVERLN TINHRYGLFS EVRGLGLLIG CVLNADYAGQ AKQISQEAAK 370 380 390 400 AGVMVLIAGG NVVRFAPALN VSEEEVTTGL DRFAAACEHF VSRGSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Escherichia coli starvation gene cstC is involved in amino acid catabolism." Fraley C.D., Kim J.H., McCann M.P., Matin A. J. Bacteriol. 180:4287-4290(1998) [PubMed: 9696780] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli." Schneider B.L., Kiupakis A.K., Reitzer L.J. J. Bacteriol. 180:4278-4286(1998) [PubMed: 9696779] [Abstract] Cited for: PROTEIN SEQUENCE OF 3-21 AND 295-309, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U90416 Genomic DNA. Translation: AAB51148.1. U00096 Genomic DNA. Translation: AAC74818.1. AP009048 Genomic DNA. Translation: BAA15539.1.
PIR	D64934.
RefSeq	NP_416262.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P77581.
SMR	P77581. Positions 15-396.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9145N.
IntAct	P77581. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004543; EBESCP00000004543; EBESCG00000003708. EBESCT00000004544; EBESCP00000004544; EBESCG00000003708. EBESCT00000004545; EBESCP00000004545; EBESCG00000003708. EBESCT00000015597; EBESCP00000014888; EBESCG00000014657.
GeneID	946255.
GenomeReviews	Gene locus JW1737 in contig AP009048_GR. Gene locus b1748 in contig U00096_GR.
KEGG	ecj:JW1737. eco:b1748.
PATRIC	32118805. VBIEscCol129921_1820.
Organism-specific databases
EchoBASE	EB3755.
EcoGene	EG13999. argM.
Phylogenomic databases
eggNOG	COG4992.
GeneTree	EBGT00050000010246.
HOGENOM	HBG725944.
OMA	GRKLWHI.
PhylomeDB	P77581.
ProtClustDB	PRK12381.
Enzyme and pathway databases
BioCyc	EcoCyc:SUCCORNTRANSAM-MONOMER. MetaCyc:SUCCORNTRANSAM-MONOMER.
Gene expression databases
Genevestigator	P77581.
Family and domain databases
HAMAP	MF_01173. AstC_aminotrans_3. [Tree]
InterPro	IPR017652. Ac/SuccinylOrn_transaminase. IPR004636. AcOrn/SuccinylOrn_aminoTrfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 2 hits.
KO	K00840.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF19. ArgD_aminotrans. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR03246. Arg_catab_astC. 1 hit. TIGR00707. ArgD. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTC_ECOLI

Accession

Primary (citable) accession number: P77581

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program