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P77581

- ASTC_ECOLI

UniProt

P77581 - ASTC_ECOLI

Protein

Succinylornithine transaminase

Gene

astC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the transamination of N(2)-succinylornithine and alpha-ketoglutarate into N(2)-succinylglutamate semialdehyde and glutamate. Can also act as an acetylornithine aminotransferase.1 Publication

    Catalytic activityi

    N(2)-succinyl-L-ornithine + 2-oxoglutarate = N-succinyl-L-glutamate 5-semialdehyde + L-glutamate.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: UniProtKB-HAMAP
    2. succinylornithine transaminase activity Source: EcoliWiki

    GO - Biological processi

    1. arginine catabolic process Source: EcoliWiki
    2. arginine catabolic process to glutamate Source: UniProtKB-HAMAP
    3. arginine catabolic process to succinate Source: EcoCyc
    4. ornithine catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Arginine metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:SUCCORNTRANSAM-MONOMER.
    ECOL316407:JW1737-MONOMER.
    MetaCyc:SUCCORNTRANSAM-MONOMER.
    UniPathwayiUPA00185; UER00281.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinylornithine transaminase (EC:2.6.1.81)
    Short name:
    SOAT
    Alternative name(s):
    Carbon starvation protein C
    Succinylornithine aminotransferase
    Gene namesi
    Name:astC
    Synonyms:argM, cstC, ydjW
    Ordered Locus Names:b1748, JW1737
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13999. argM.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 406406Succinylornithine transaminasePRO_0000120352Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei252 – 2521N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PaxDbiP77581.
    PRIDEiP77581.

    Expressioni

    Gene expression databases

    GenevestigatoriP77581.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    groLP0A6F51EBI-1125336,EBI-543750

    Protein-protein interaction databases

    DIPiDIP-9145N.
    IntActiP77581. 2 interactions.
    STRINGi511145.b1748.

    Structurei

    Secondary structure

    1
    406
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 137
    Beta strandi25 – 306
    Beta strandi32 – 354
    Beta strandi40 – 456
    Helixi46 – 494
    Helixi58 – 6811
    Helixi81 – 9313
    Beta strandi97 – 1048
    Helixi105 – 12319
    Beta strandi130 – 1345
    Helixi143 – 1486
    Helixi152 – 1543
    Helixi156 – 1583
    Beta strandi163 – 1686
    Helixi173 – 1775
    Beta strandi184 – 1896
    Beta strandi191 – 1933
    Turni194 – 1974
    Beta strandi198 – 2003
    Helixi203 – 21513
    Beta strandi219 – 2235
    Turni225 – 2328
    Beta strandi233 – 2364
    Helixi237 – 2415
    Beta strandi246 – 2505
    Helixi252 – 2554
    Beta strandi261 – 2655
    Helixi267 – 2715
    Helixi286 – 29914
    Helixi302 – 32625
    Beta strandi329 – 3357
    Beta strandi338 – 3436
    Turni345 – 3495
    Helixi351 – 36010
    Beta strandi366 – 3694
    Beta strandi372 – 3754
    Helixi383 – 40119

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4ADBX-ray2.20A/B/C/D1-406[»]
    4ADCX-ray2.30A/B/C/D1-406[»]
    4ADDX-ray2.45A/B/C/D1-406[»]
    4ADEX-ray2.75A/B1-406[»]
    ProteinModelPortaliP77581.
    SMRiP77581. Positions 3-403.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG4992.
    HOGENOMiHOG000020206.
    KOiK00840.
    OMAiASNAFLH.
    OrthoDBiEOG6QVRHN.
    PhylomeDBiP77581.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    HAMAPiMF_01107. ArgD_aminotrans_3.
    MF_01173. AstC_aminotrans_3.
    InterProiIPR017652. Ac/SucOrn_transaminase_bac.
    IPR004636. AcOrn/SuccOrn_fam.
    IPR005814. Aminotrans_3.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR026330. SOAT.
    [Graphical view]
    PANTHERiPTHR11986. PTHR11986. 1 hit.
    PfamiPF00202. Aminotran_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR03246. arg_catab_astC. 1 hit.
    TIGR00707. argD. 1 hit.
    PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P77581-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQPITRENF DEWMIPVYAP APFIPVRGEG SRLWDQQGKE YIDFAGGIAV    50
    NALGHAHPEL REALNEQASK FWHTGNGYTN EPVLRLAKKL IDATFADRVF 100
    FCNSGAEANE AALKLARKFA HDRYGSHKSG IVAFKNAFHG RTLFTVSAGG 150
    QPAYSQDFAP LPADIRHAAY NDINSASALI DDSTCAVIVE PIQGEGGVVP 200
    ASNAFLQGLR ELCNRHNALL IFDEVQTGVG RTGELYAYMH YGVTPDLLTT 250
    AKALGGGFPV GALLATEECA RVMTVGTHGT TYGGNPLASA VAGKVLELIN 300
    TPEMLNGVKQ RHDWFVERLN TINHRYGLFS EVRGLGLLIG CVLNADYAGQ 350
    AKQISQEAAK AGVMVLIAGG NVVRFAPALN VSEEEVTTGL DRFAAACEHF 400
    VSRGSS 406
    Length:406
    Mass (Da):43,665
    Last modified:February 1, 1997 - v1
    Checksum:iB18ED13644C21176
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti361 – 3611A → R in AAB51148. (PubMed:9696780)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U90416 Genomic DNA. Translation: AAB51148.1.
    U00096 Genomic DNA. Translation: AAC74818.1.
    AP009048 Genomic DNA. Translation: BAA15539.1.
    PIRiD64934.
    RefSeqiNP_416262.1. NC_000913.3.
    YP_490009.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74818; AAC74818; b1748.
    BAA15539; BAA15539; BAA15539.
    GeneIDi12934011.
    946255.
    KEGGiecj:Y75_p1723.
    eco:b1748.
    PATRICi32118805. VBIEscCol129921_1820.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U90416 Genomic DNA. Translation: AAB51148.1 .
    U00096 Genomic DNA. Translation: AAC74818.1 .
    AP009048 Genomic DNA. Translation: BAA15539.1 .
    PIRi D64934.
    RefSeqi NP_416262.1. NC_000913.3.
    YP_490009.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4ADB X-ray 2.20 A/B/C/D 1-406 [» ]
    4ADC X-ray 2.30 A/B/C/D 1-406 [» ]
    4ADD X-ray 2.45 A/B/C/D 1-406 [» ]
    4ADE X-ray 2.75 A/B 1-406 [» ]
    ProteinModelPortali P77581.
    SMRi P77581. Positions 3-403.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9145N.
    IntActi P77581. 2 interactions.
    STRINGi 511145.b1748.

    Proteomic databases

    PaxDbi P77581.
    PRIDEi P77581.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74818 ; AAC74818 ; b1748 .
    BAA15539 ; BAA15539 ; BAA15539 .
    GeneIDi 12934011.
    946255.
    KEGGi ecj:Y75_p1723.
    eco:b1748.
    PATRICi 32118805. VBIEscCol129921_1820.

    Organism-specific databases

    EchoBASEi EB3755.
    EcoGenei EG13999. argM.

    Phylogenomic databases

    eggNOGi COG4992.
    HOGENOMi HOG000020206.
    KOi K00840.
    OMAi ASNAFLH.
    OrthoDBi EOG6QVRHN.
    PhylomeDBi P77581.

    Enzyme and pathway databases

    UniPathwayi UPA00185 ; UER00281 .
    BioCyci EcoCyc:SUCCORNTRANSAM-MONOMER.
    ECOL316407:JW1737-MONOMER.
    MetaCyc:SUCCORNTRANSAM-MONOMER.

    Miscellaneous databases

    PROi P77581.

    Gene expression databases

    Genevestigatori P77581.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    HAMAPi MF_01107. ArgD_aminotrans_3.
    MF_01173. AstC_aminotrans_3.
    InterProi IPR017652. Ac/SucOrn_transaminase_bac.
    IPR004636. AcOrn/SuccOrn_fam.
    IPR005814. Aminotrans_3.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR026330. SOAT.
    [Graphical view ]
    PANTHERi PTHR11986. PTHR11986. 1 hit.
    Pfami PF00202. Aminotran_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR03246. arg_catab_astC. 1 hit.
    TIGR00707. argD. 1 hit.
    PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Escherichia coli starvation gene cstC is involved in amino acid catabolism."
      Fraley C.D., Kim J.H., McCann M.P., Matin A.
      J. Bacteriol. 180:4287-4290(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli."
      Schneider B.L., Kiupakis A.K., Reitzer L.J.
      J. Bacteriol. 180:4278-4286(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 3-21 AND 295-309, FUNCTION.

    Entry informationi

    Entry nameiASTC_ECOLI
    AccessioniPrimary (citable) accession number: P77581
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3