Reviewed, UniProtKB/Swiss-Prot P77581 (ASTC_ECOLI)

Names and origin

Protein names

Recommended name:
    Succinylornithine transaminase
      Short name=SOAT
    EC=2.6.1.81
Alternative name(s):
    Succinylornithine aminotransferase
    Carbon starvation protein C

Gene names

Name:	astC
Synonyms:	argM, cstC, ydjW
Ordered Locus Names:	b1748, JW1737

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transamination of N(2)-succinylornithine and alpha-ketoglutarate into N(2)-succinylglutamate semialdehyde and glutamate. Can also act as an acetylornithine aminotransferase. Ref.5
Catalytic activity	N(2)-succinyl-L-ornithine + 2-oxoglutarate = N-succinyl-L-glutamate 5-semialdehyde + L-glutamate. HAMAP MF_01173
Cofactor	Pyridoxal phosphate. HAMAP MF_01173
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 3/5. HAMAP MF_01173
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. AstC subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	protein binding Inferred from physical interaction. Source: IntAct pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP succinylornithine transaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-1125336,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Succinylornithine transaminase HAMAP MF_01173		PRO_0000120352
Amino acid modifications
	Modified residue	252	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	361	1	A → R in AAB51148. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P77581-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B18ED13644C21176	FASTA	406	43,665
	10 20 30 40 50 60 MSQPITRENF DEWMIPVYAP APFIPVRGEG SRLWDQQGKE YIDFAGGIAV NALGHAHPEL 70 80 90 100 110 120 REALNEQASK FWHTGNGYTN EPVLRLAKKL IDATFADRVF FCNSGAEANE AALKLARKFA 130 140 150 160 170 180 HDRYGSHKSG IVAFKNAFHG RTLFTVSAGG QPAYSQDFAP LPADIRHAAY NDINSASALI 190 200 210 220 230 240 DDSTCAVIVE PIQGEGGVVP ASNAFLQGLR ELCNRHNALL IFDEVQTGVG RTGELYAYMH 250 260 270 280 290 300 YGVTPDLLTT AKALGGGFPV GALLATEECA RVMTVGTHGT TYGGNPLASA VAGKVLELIN 310 320 330 340 350 360 TPEMLNGVKQ RHDWFVERLN TINHRYGLFS EVRGLGLLIG CVLNADYAGQ AKQISQEAAK 370 380 390 400 AGVMVLIAGG NVVRFAPALN VSEEEVTTGL DRFAAACEHF VSRGSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Escherichia coli starvation gene cstC is involved in amino acid catabolism." Fraley C.D., Kim J.H., McCann M.P., Matin A. J. Bacteriol. 180:4287-4290(1998) [PubMed: 9696780] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli." Schneider B.L., Kiupakis A.K., Reitzer L.J. J. Bacteriol. 180:4278-4286(1998) [PubMed: 9696779] [Abstract] Cited for: PROTEIN SEQUENCE OF 3-21 AND 295-309, FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U90416 Genomic DNA. Translation: AAB51148.1. U00096 Genomic DNA. Translation: AAC74818.1. AP009048 Genomic DNA. Translation: BAA15539.1.
PIR	D64934.
RefSeq	AP_002367.1. NP_416262.1.
3D structure databases
HSSP	HSSP built from PDB template 1QJ3 based on UniProtKB P12995.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77581. 1 interaction.
Genome annotation databases
GeneID	946255.
GenomeReviews	Gene locus JW1737 in contig AP009048_GR. Gene locus b1748 in contig U00096_GR.
KEGG	ecj:JW1737. eco:b1748.
Organism-specific databases
EchoBASE	EB3755.
EcoGene	EG13999. argM.
CMR	Search...
Phylogenomic databases
HOGENOM	P77581.
OMA	P77581. GRKLWHI.
Enzyme and pathway databases
BioCyc	EcoCyc:SUCCORNTRANSAM-MON. MetaCyc:SUCCORNTRANSAM-MON.
Family and domain databases
HAMAP	MF_01173. [Tree]
InterPro	IPR005814. Aminotrans_3. IPR004636. ArgD_aminotrans. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR017652. SuccinylOrn_transaminase. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF19. ArgD_aminotrans. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR03246. arg_catab_astC. 1 hit. TIGR00707. argD. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTC_ECOLI

Accession

Primary (citable) accession number: P77581

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)