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P77580

- ACDH_ECOLI

UniProt

P77580 - ACDH_ECOLI

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Protein
Acetaldehyde dehydrogenase
Gene
mhpF, mhpE, b0351, JW0342
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD+ and coenzyme A. Is the final enzyme in the meta-cleavage pathway for the degradation of 3-phenylpropanoate. Functions as a chaperone protein for folding of MhpE.1 Publication

Catalytic activityi

Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei131 – 1311Acyl-thioester intermediate By similarity
Binding sitei289 – 2891NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 144NAD By similarity
Nucleotide bindingi162 – 1709NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: UniProtKB-HAMAP
  2. acetaldehyde dehydrogenase (acetylating) activity Source: EcoliWiki
  3. protein binding Source: EcoliWiki

GO - Biological processi

  1. 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:MHPF-MONOMER.
ECOL316407:JW0342-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetaldehyde dehydrogenase (EC:1.2.1.10)
Alternative name(s):
Acetaldehyde dehydrogenase [acetylating]
Gene namesi
Name:mhpF
Synonyms:mhpE
Ordered Locus Names:b0351, JW0342
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13625. mhpF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Acetaldehyde dehydrogenaseUniRule annotation
PRO_0000096471Add
BLAST

Interactioni

Subunit structurei

Interacts with MhpE.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
mhpEP510201EBI-1116083,EBI-1116093

Protein-protein interaction databases

DIPiDIP-10210N.
IntActiP77580. 18 interactions.
STRINGi511145.b0351.

Structurei

3D structure databases

ProteinModelPortaliP77580.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4569.
HOGENOMiHOG000052149.
KOiK04073.
OMAiHQGNVNM.
OrthoDBiEOG6H1PXH.
PhylomeDBiP77580.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01657. Ac_ald_DH_ac.
InterProiIPR003361. Acetaldehyde_dehydrogenase.
IPR015426. Acetylaldehyde_DH_C.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view]
PfamiPF09290. AcetDehyd-dimer. 1 hit.
PF01118. Semialdhyde_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015689. Actaldh_dh_actl. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03215. ac_ald_DH_ac. 1 hit.

Sequencei

Sequence statusi: Complete.

P77580-1 [UniParc]FASTAAdd to Basket

« Hide

MSKRKVAIIG SGNIGTDLMI KILRHGQHLE MAVMVGIDPQ SDGLARARRM    50
GVATTHEGVI GLMNMPEFAD IDIVFDATSA GAHVKNDAAL REAKPDIRLI 100
DLTPAAIGPY CVPVVNLEAN VDQLNVNMVT CGGQATIPMV AAVSRVARVH 150
YAEIIASIAS KSAGPGTRAN IDEFTETTSR AIEVVGGAAK GKAIIVLNPA 200
EPPLMMRDTV YVLSDEASQD DIEASINEMA EAVQAYVPGY RLKQRVQFEV 250
IPQDKPVNLP GVGQFSGLKT AVWLEVEGAA HYLPAYAGNL DIMTSSALAT 300
AEKMAQSLAR KAGEAA 316
Length:316
Mass (Da):33,442
Last modified:February 1, 1997 - v1
Checksum:iA6918BDA5EF4876B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86239 Genomic DNA. Translation: BAA13056.1.
Y09555 Genomic DNA. Translation: CAA70751.1.
U73857 Genomic DNA. Translation: AAB18075.1.
U00096 Genomic DNA. Translation: AAC73454.1.
AP009048 Genomic DNA. Translation: BAE76133.1.
PIRiG64762.
RefSeqiNP_414885.1. NC_000913.3.
YP_488645.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73454; AAC73454; b0351.
BAE76133; BAE76133; BAE76133.
GeneIDi12932628.
945008.
KEGGiecj:Y75_p0340.
eco:b0351.
PATRICi32115835. VBIEscCol129921_0359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86239 Genomic DNA. Translation: BAA13056.1 .
Y09555 Genomic DNA. Translation: CAA70751.1 .
U73857 Genomic DNA. Translation: AAB18075.1 .
U00096 Genomic DNA. Translation: AAC73454.1 .
AP009048 Genomic DNA. Translation: BAE76133.1 .
PIRi G64762.
RefSeqi NP_414885.1. NC_000913.3.
YP_488645.1. NC_007779.1.

3D structure databases

ProteinModelPortali P77580.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10210N.
IntActi P77580. 18 interactions.
STRINGi 511145.b0351.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73454 ; AAC73454 ; b0351 .
BAE76133 ; BAE76133 ; BAE76133 .
GeneIDi 12932628.
945008.
KEGGi ecj:Y75_p0340.
eco:b0351.
PATRICi 32115835. VBIEscCol129921_0359.

Organism-specific databases

EchoBASEi EB3390.
EcoGenei EG13625. mhpF.

Phylogenomic databases

eggNOGi COG4569.
HOGENOMi HOG000052149.
KOi K04073.
OMAi HQGNVNM.
OrthoDBi EOG6H1PXH.
PhylomeDBi P77580.

Enzyme and pathway databases

UniPathwayi UPA00714 .
BioCyci EcoCyc:MHPF-MONOMER.
ECOL316407:JW0342-MONOMER.

Miscellaneous databases

PROi P77580.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_01657. Ac_ald_DH_ac.
InterProi IPR003361. Acetaldehyde_dehydrogenase.
IPR015426. Acetylaldehyde_DH_C.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view ]
Pfami PF09290. AcetDehyd-dimer. 1 hit.
PF01118. Semialdhyde_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF015689. Actaldh_dh_actl. 1 hit.
SMARTi SM00859. Semialdhyde_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR03215. ac_ald_DH_ac. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of the mhp operon."
    Kawamukai M.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12."
    Ferrandez A., Garcia J.L., Diaz E.
    J. Bacteriol. 179:2573-2581(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / CS520.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli."
    Lee S.J., Ko J.H., Kang H.Y., Lee Y.
    Biochem. Biophys. Res. Commun. 346:1009-1015(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MHPF, FUNCTION.

Entry informationi

Entry nameiACDH_ECOLI
AccessioniPrimary (citable) accession number: P77580
Secondary accession number(s): Q2MC73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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