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Reviewed, UniProtKB/Swiss-Prot P77580 (ACDH_ECOLI)

Last modified July 13, 2010. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
Acetaldehyde dehydrogenase
EC=1.2.1.10
Alternative name(s):
Acetaldehyde dehydrogenase [acetylating]
Gene names
Name:mhpF
Synonyms:mhpE
Ordered Locus Names:b0351, JW0342
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia
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Protein attributesHide

Sequence length316 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Function

Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD+ and coenzyme A. Is the final enzyme in the meta-cleavage pathway for the degradation of 3-phenylpropanoate. Functions as a chaperone protein for folding of mhpE. Ref.6

Catalytic activity

Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH. HAMAP MF_01657

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01657

Subunit structure

Interacts with mhpE. Ref.6

Sequence similarities

Belongs to the acetaldehyde dehydrogenase family.

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Binary interactionsHide

With

Entry

#Exp.

IntAct

Notes

mhpEP510201EBI-1116083,EBI-1116093
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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Acetaldehyde dehydrogenase HAMAP MF_01657
PRO_0000096471
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SequencesHide

Sequence LengthMass (Da)Tools
P77580-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: A6918BDA5EF4876B

FASTA31633,442
        10         20         30         40         50         60 
MSKRKVAIIG SGNIGTDLMI KILRHGQHLE MAVMVGIDPQ SDGLARARRM GVATTHEGVI 

        70         80         90        100        110        120 
GLMNMPEFAD IDIVFDATSA GAHVKNDAAL REAKPDIRLI DLTPAAIGPY CVPVVNLEAN 

       130        140        150        160        170        180 
VDQLNVNMVT CGGQATIPMV AAVSRVARVH YAEIIASIAS KSAGPGTRAN IDEFTETTSR 

       190        200        210        220        230        240 
AIEVVGGAAK GKAIIVLNPA EPPLMMRDTV YVLSDEASQD DIEASINEMA EAVQAYVPGY 

       250        260        270        280        290        300 
RLKQRVQFEV IPQDKPVNLP GVGQFSGLKT AVWLEVEGAA HYLPAYAGNL DIMTSSALAT 

       310 
AEKMAQSLAR KAGEAA

« Hide

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ReferencesHide

« Hide 'large scale' references
[1]Kawamukai M.
Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12."
Ferrandez A., Garcia J.L., Diaz E.
J. Bacteriol. 179:2573-2581(1997) [PubMed: 9098055] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / CS520.
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli."
Lee S.J., Ko J.H., Kang H.Y., Lee Y.
Biochem. Biophys. Res. Commun. 346:1009-1015(2006) [PubMed: 16782065] [Abstract]
Cited for: INTERACTION WITH MHPF, FUNCTION.
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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ		D86239 Genomic DNA. Translation: BAA13056.1.
Y09555 Genomic DNA. Translation: CAA70751.1.
U73857 Genomic DNA. Translation: AAB18075.1.
U00096 Genomic DNA. Translation: AAC73454.1.
AP009048 Genomic DNA. Translation: BAE76133.1.
PIRG64762.
RefSeqAP_001003.1.
NP_414885.1.

3D structure databases

SMRP77580. Positions 3-308.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10210N.
IntActP77580. 5 interactions.
STRINGP77580.

Genome annotation databases

EnsemblBacteriaEBESCT00000002318; EBESCP00000002318; EBESCG00000001893.
EBESCT00000017431; EBESCP00000016722; EBESCG00000016487.
GeneID945008.
GenomeReviewsGene locus JW0342 in contig AP009048_GR.
Gene locus b0351 in contig U00096_GR.
KEGGecj:JW0342.
eco:b0351.

Organism-specific databases

EchoBASEEB3390.
EcoGeneEG13625. mhpF.
CMRSearch...

Phylogenomic databases

eggNOGCOG4569.
HOGENOMHBG300125.
OMAHVKNDAF.
ProtClustDBPRK08300.

Enzyme and pathway databases

BioCycEcoCyc:MHPF-MONOMER.
ECOL168927:B0351-MONOMER.
MetaCyc:MHPF-MONOMER.

Family and domain databases

HAMAPMF_01657. Ac_ald_DH_ac.
[Tree]
InterProIPR003361. Acetaldehyde_dehydrogenase.
IPR015426. Acetylaldehyde_DH_C.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view]
PANTHERPTHR21123. Acetylald_dh. 1 hit.
PfamPF09290. AcetDehyd-dimer. 1 hit.
PF01118. Semialdhyde_dh. 1 hit.
[Graphical view]
PIRSFPIRSF015689. Actaldh_dh_actl. 1 hit.
SMARTSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR03215. ac_ald_DH_ac. 1 hit.
ProtoNetSearch...
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Entry informationHide

Entry nameACDH_ECOLI
AccessionPrimary (citable) accession number: P77580
Secondary accession number(s): Q2MC73
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 13, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)
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Relevant documentsHide

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents