Reviewed, UniProtKB/Swiss-Prot P77580 (MHPF_ECOLI)

Names and origin

Protein names

Recommended name:
    Acetaldehyde dehydrogenase
    EC=1.2.1.10
Alternative name(s):
    Acetaldehyde dehydrogenase [acetylating]

Gene names

Name:	mhpF
Synonyms:	mhpE
Ordered Locus Names:	b0351, JW0342

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the terminal reaction in meta-cleavage pathways, that is, the transformation of acetaldehyde into acetyl coenzyme A. Functions as a chaperone protein for folding of mhpE. Ref.6
Catalytic activity	Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH. HAMAP MF_01657
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation. HAMAP MF_01657
Subunit structure	Interacts with mhpE. Ref.6
Sequence similarities	Belongs to the acetaldehyde dehydrogenase family. MhpF subfamily.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro acetaldehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP protein binding Ref.6 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mhpE	P51020	1	EBI-1116083,EBI-1116093

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Acetaldehyde dehydrogenase HAMAP MF_01657		PRO_0000096471

Sequences

	Sequence		Length	Mass (Da)
	P77580-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: A6918BDA5EF4876B	FASTA	316	33,442
	10 20 30 40 50 60 MSKRKVAIIG SGNIGTDLMI KILRHGQHLE MAVMVGIDPQ SDGLARARRM GVATTHEGVI 70 80 90 100 110 120 GLMNMPEFAD IDIVFDATSA GAHVKNDAAL REAKPDIRLI DLTPAAIGPY CVPVVNLEAN 130 140 150 160 170 180 VDQLNVNMVT CGGQATIPMV AAVSRVARVH YAEIIASIAS KSAGPGTRAN IDEFTETTSR 190 200 210 220 230 240 AIEVVGGAAK GKAIIVLNPA EPPLMMRDTV YVLSDEASQD DIEASINEMA EAVQAYVPGY 250 260 270 280 290 300 RLKQRVQFEV IPQDKPVNLP GVGQFSGLKT AVWLEVEGAA HYLPAYAGNL DIMTSSALAT 310 AEKMAQSLAR KAGEAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kawamukai M. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12." Ferrandez A., Garcia J.L., Diaz E. J. Bacteriol. 179:2573-2581(1997) [PubMed: 9098055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / CS520.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli." Lee S.J., Ko J.H., Kang H.Y., Lee Y. Biochem. Biophys. Res. Commun. 346:1009-1015(2006) [PubMed: 16782065] [Abstract] Cited for: INTERACTION WITH MHPF, FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86239 Genomic DNA. Translation: BAA13056.1. Y09555 Genomic DNA. Translation: CAA70751.1. U73857 Genomic DNA. Translation: AAB18075.1. U00096 Genomic DNA. Translation: AAC73454.1. AP009048 Genomic DNA. Translation: BAE76133.1.
PIR	G64762.
RefSeq	AP_001003.1. NP_414885.1.
3D structure databases
SMR	P77580. Positions 3-308.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77580. 5 interactions.
Genome annotation databases
GeneID	945008.
GenomeReviews	Gene locus JW0342 in contig AP009048_GR. Gene locus b0351 in contig U00096_GR.
KEGG	ecj:JW0342. eco:b0351.
Organism-specific databases
EchoBASE	EB3390.
EcoGene	EG13625. mhpF.
CMR	Search...
Phylogenomic databases
HOGENOM	P77580.
OMA	P77580. HVKNDAF.
Enzyme and pathway databases
BioCyc	EcoCyc:MHPF-MON. MetaCyc:MHPF-MON.
Family and domain databases
HAMAP	MF_01657. [Tree]
InterPro	IPR003361. Acetaldehyde_dehydrogenase. IPR015426. Acetylaldehyde_DH_C. IPR000534. Semialdehyde_DH_NAD-bd. [Graphical view]
PANTHER	PTHR21123. Acetylald_dh. 1 hit.
Pfam	PF09290. AcetDehyd-dimer. 1 hit. PF01118. Semialdhyde_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015689. Actaldh_dh_actl. 1 hit.
TIGRFAMs	TIGR03215. ac_ald_DH_ac. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MHPF_ECOLI

Accession

Primary (citable) accession number: P77580
Secondary accession number(s): Q2MC73

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)