P77580 (ACDH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetaldehyde dehydrogenase
EC=1.2.1.10

Alternative name(s):
Acetaldehyde dehydrogenase [acetylating]

Gene names

Name:	mhpF
Synonyms:	mhpE
Ordered Locus Names:	b0351, JW0342

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD+ and coenzyme A. Is the final enzyme in the meta-cleavage pathway for the degradation of 3-phenylpropanoate. Functions as a chaperone protein for folding of MhpE. Ref.6
Catalytic activity	Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH. HAMAP MF_01657
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01657
Subunit structure	Interacts with MhpE. Ref.6
Sequence similarities	Belongs to the acetaldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro acetaldehyde dehydrogenase (acetylating) activity Inferred from direct assay. Source: EcoliWiki
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mhpE	P51020	1	EBI-1116083,EBI-1116093

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Acetaldehyde dehydrogenase HAMAP MF_01657		PRO_0000096471
Regions
	Nucleotide binding	11 – 14	4	NAD By similarity
	Nucleotide binding	162 – 170	9	NAD By similarity
Sites
	Binding site	289	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77580 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: A6918BDA5EF4876B	FASTA	316	33,442
	10 20 30 40 50 60 MSKRKVAIIG SGNIGTDLMI KILRHGQHLE MAVMVGIDPQ SDGLARARRM GVATTHEGVI 70 80 90 100 110 120 GLMNMPEFAD IDIVFDATSA GAHVKNDAAL REAKPDIRLI DLTPAAIGPY CVPVVNLEAN 130 140 150 160 170 180 VDQLNVNMVT CGGQATIPMV AAVSRVARVH YAEIIASIAS KSAGPGTRAN IDEFTETTSR 190 200 210 220 230 240 AIEVVGGAAK GKAIIVLNPA EPPLMMRDTV YVLSDEASQD DIEASINEMA EAVQAYVPGY 250 260 270 280 290 300 RLKQRVQFEV IPQDKPVNLP GVGQFSGLKT AVWLEVEGAA HYLPAYAGNL DIMTSSALAT 310 AEKMAQSLAR KAGEAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kawamukai M. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12." Ferrandez A., Garcia J.L., Diaz E. J. Bacteriol. 179:2573-2581(1997) [PubMed: 9098055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / CS520.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli." Lee S.J., Ko J.H., Kang H.Y., Lee Y. Biochem. Biophys. Res. Commun. 346:1009-1015(2006) [PubMed: 16782065] [Abstract] Cited for: INTERACTION WITH MHPF, FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86239 Genomic DNA. Translation: BAA13056.1. Y09555 Genomic DNA. Translation: CAA70751.1. U73857 Genomic DNA. Translation: AAB18075.1. U00096 Genomic DNA. Translation: AAC73454.1. AP009048 Genomic DNA. Translation: BAE76133.1.
PIR	G64762.
RefSeq	NP_414885.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P77580.
SMR	P77580. Positions 3-308.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10210N.
IntAct	P77580. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002318; EBESCP00000002318; EBESCG00000001893. EBESCT00000017431; EBESCP00000016722; EBESCG00000016487.
GeneID	945008.
GenomeReviews	Gene locus JW0342 in contig AP009048_GR. Gene locus b0351 in contig U00096_GR.
KEGG	ecj:JW0342. eco:b0351.
PATRIC	32115835. VBIEscCol129921_0359.
Organism-specific databases
EchoBASE	EB3390.
EcoGene	EG13625. mhpF.
Phylogenomic databases
eggNOG	COG4569.
GeneTree	EBGT00050000011515.
HOGENOM	HBG300125.
OMA	HVKNDAF.
PhylomeDB	P77580.
ProtClustDB	PRK08300.
Enzyme and pathway databases
BioCyc	EcoCyc:MHPF-MONOMER. MetaCyc:MHPF-MONOMER.
Family and domain databases
HAMAP	MF_01657. Ac_ald_DH_ac. [Tree]
InterPro	IPR003361. Acetaldehyde_dehydrogenase. IPR015426. Acetylaldehyde_DH_C. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K04073.
PANTHER	PTHR21123. Acetylald_dh. 1 hit.
Pfam	PF09290. AcetDehyd-dimer. 1 hit. PF01118. Semialdhyde_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015689. Actaldh_dh_actl. 1 hit.
SMART	SM00859. Semialdhyde_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03215. Ac_ald_DH_ac. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACDH_ECOLI

Accession

Primary (citable) accession number: P77580
Secondary accession number(s): Q2MC73

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program