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Protein

Acetaldehyde dehydrogenase

Gene

mhpF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD+ and coenzyme A. Is the final enzyme in the meta-cleavage pathway for the degradation of 3-phenylpropanoate. Functions as a chaperone protein for folding of MhpE.1 Publication

Catalytic activityi

Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.

Pathway: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei131 – 1311Acyl-thioester intermediateBy similarity
Binding sitei289 – 2891NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 144NADBy similarity
Nucleotide bindingi162 – 1709NADBy similarity

GO - Molecular functioni

  • acetaldehyde dehydrogenase (acetylating) activity Source: EcoliWiki
  • NAD binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:MHPF-MONOMER.
ECOL316407:JW0342-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetaldehyde dehydrogenase (EC:1.2.1.10)
Alternative name(s):
Acetaldehyde dehydrogenase [acetylating]
Gene namesi
Name:mhpF
Synonyms:mhpE
Ordered Locus Names:b0351, JW0342
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13625. mhpF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Acetaldehyde dehydrogenasePRO_0000096471Add
BLAST

Interactioni

Subunit structurei

Interacts with MhpE.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
mhpEP510201EBI-1116083,EBI-1116093

Protein-protein interaction databases

DIPiDIP-10210N.
IntActiP77580. 18 interactions.
STRINGi511145.b0351.

Structurei

3D structure databases

ProteinModelPortaliP77580.
SMRiP77580. Positions 3-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetaldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG4569.
HOGENOMiHOG000052149.
InParanoidiP77580.
KOiK04073.
OMAiHQGNVNM.
OrthoDBiEOG6H1PXH.
PhylomeDBiP77580.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01657. Ac_ald_DH_ac.
InterProiIPR003361. Acetaldehyde_dehydrogenase.
IPR015426. Acetylaldehyde_DH_C.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view]
PfamiPF09290. AcetDehyd-dimer. 1 hit.
PF01118. Semialdhyde_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015689. Actaldh_dh_actl. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03215. ac_ald_DH_ac. 1 hit.

Sequencei

Sequence statusi: Complete.

P77580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRKVAIIG SGNIGTDLMI KILRHGQHLE MAVMVGIDPQ SDGLARARRM
60 70 80 90 100
GVATTHEGVI GLMNMPEFAD IDIVFDATSA GAHVKNDAAL REAKPDIRLI
110 120 130 140 150
DLTPAAIGPY CVPVVNLEAN VDQLNVNMVT CGGQATIPMV AAVSRVARVH
160 170 180 190 200
YAEIIASIAS KSAGPGTRAN IDEFTETTSR AIEVVGGAAK GKAIIVLNPA
210 220 230 240 250
EPPLMMRDTV YVLSDEASQD DIEASINEMA EAVQAYVPGY RLKQRVQFEV
260 270 280 290 300
IPQDKPVNLP GVGQFSGLKT AVWLEVEGAA HYLPAYAGNL DIMTSSALAT
310
AEKMAQSLAR KAGEAA
Length:316
Mass (Da):33,442
Last modified:February 1, 1997 - v1
Checksum:iA6918BDA5EF4876B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA. Translation: BAA13056.1.
Y09555 Genomic DNA. Translation: CAA70751.1.
U73857 Genomic DNA. Translation: AAB18075.1.
U00096 Genomic DNA. Translation: AAC73454.1.
AP009048 Genomic DNA. Translation: BAE76133.1.
PIRiG64762.
RefSeqiNP_414885.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73454; AAC73454; b0351.
BAE76133; BAE76133; BAE76133.
GeneIDi945008.
KEGGiecj:Y75_p0340.
eco:b0351.
PATRICi32115835. VBIEscCol129921_0359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA. Translation: BAA13056.1.
Y09555 Genomic DNA. Translation: CAA70751.1.
U73857 Genomic DNA. Translation: AAB18075.1.
U00096 Genomic DNA. Translation: AAC73454.1.
AP009048 Genomic DNA. Translation: BAE76133.1.
PIRiG64762.
RefSeqiNP_414885.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP77580.
SMRiP77580. Positions 3-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10210N.
IntActiP77580. 18 interactions.
STRINGi511145.b0351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73454; AAC73454; b0351.
BAE76133; BAE76133; BAE76133.
GeneIDi945008.
KEGGiecj:Y75_p0340.
eco:b0351.
PATRICi32115835. VBIEscCol129921_0359.

Organism-specific databases

EchoBASEiEB3390.
EcoGeneiEG13625. mhpF.

Phylogenomic databases

eggNOGiCOG4569.
HOGENOMiHOG000052149.
InParanoidiP77580.
KOiK04073.
OMAiHQGNVNM.
OrthoDBiEOG6H1PXH.
PhylomeDBiP77580.

Enzyme and pathway databases

UniPathwayiUPA00714.
BioCyciEcoCyc:MHPF-MONOMER.
ECOL316407:JW0342-MONOMER.

Miscellaneous databases

PROiP77580.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01657. Ac_ald_DH_ac.
InterProiIPR003361. Acetaldehyde_dehydrogenase.
IPR015426. Acetylaldehyde_DH_C.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view]
PfamiPF09290. AcetDehyd-dimer. 1 hit.
PF01118. Semialdhyde_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015689. Actaldh_dh_actl. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03215. ac_ald_DH_ac. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of the mhp operon."
    Kawamukai M.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12."
    Ferrandez A., Garcia J.L., Diaz E.
    J. Bacteriol. 179:2573-2581(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / CS520.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli."
    Lee S.J., Ko J.H., Kang H.Y., Lee Y.
    Biochem. Biophys. Res. Commun. 346:1009-1015(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MHPF, FUNCTION.

Entry informationi

Entry nameiACDH_ECOLI
AccessioniPrimary (citable) accession number: P77580
Secondary accession number(s): Q2MC73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 27, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.