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Protein

Anhydro-N-acetylmuramic acid kinase

Gene

anmK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.2 Publications

Catalytic activityi

ATP + 1,6-anhydro-N-acetyl-beta-muramate + H2O = ADP + N-acetylmuramate 6-phosphate.1 Publication

Enzyme regulationi

Strongly inhibited by ADP.1 Publication

Kineticsi

  1. KM=1 mM for anhMurNAc1 Publication
  2. KM=1 mM for ATP1 Publication
  1. Vmax=180 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 10-11. Inactive at pH 4.5.1 Publication

Pathwayi: 1,6-anhydro-N-acetylmuramate degradation

This protein is involved in the pathway 1,6-anhydro-N-acetylmuramate degradation, which is part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the pathway 1,6-anhydro-N-acetylmuramate degradation and in Amino-sugar metabolism.

Pathwayi: peptidoglycan recycling

This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 198ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • kinase activity Source: EcoCyc
  • phosphotransferase activity, alcohol group as acceptor Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6880-MONOMER.
ECOL316407:JW1632-MONOMER.
MetaCyc:G6880-MONOMER.
SABIO-RKP77570.
UniPathwayiUPA00343.
UPA00544.

Names & Taxonomyi

Protein namesi
Recommended name:
Anhydro-N-acetylmuramic acid kinase (EC:2.7.1.170)
Alternative name(s):
AnhMurNAc kinase
Gene namesi
Name:anmK
Synonyms:ydhH
Ordered Locus Names:b1640, JW1632
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13942. anmK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Anhydro-N-acetylmuramic acid kinasePRO_0000214821Add
BLAST

Proteomic databases

PaxDbiP77570.
PRIDEiP77570.

Interactioni

Protein-protein interaction databases

BioGridi4263491. 6 interactions.
IntActiP77570. 7 interactions.
STRINGi511145.b1640.

Structurei

3D structure databases

ProteinModelPortaliP77570.
SMRiP77570. Positions 5-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105ED7. Bacteria.
COG2377. LUCA.
HOGENOMiHOG000256309.
InParanoidiP77570.
KOiK09001.
OMAiGCHGQTI.
PhylomeDBiP77570.

Family and domain databases

HAMAPiMF_01270. AnhMurNAc_kinase. 1 hit.
InterProiIPR005338. Anhydro_N_Ac-Mur_kinase.
[Graphical view]
PfamiPF03702. UPF0075. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSGRFIGVM SGTSLDGVDV VLATIDEHRV AQLASLSWPI PVSLKQAVLD
60 70 80 90 100
ICQGQQLTLS QFGQLDTQLG QLFADAVNAL LKEQNLQARD IVAIGCHGQT
110 120 130 140 150
VWHEPTGVAP HTLQIGDNNQ IVARTGITVV GDFRRRDIAL GGQGAPLVPA
160 170 180 190 200
FHHALLAHPT ERRMVLNIGG IANLSLLIPG QPVGGYDTGP GNMLMDAWIW
210 220 230 240 250
RQAGKPYDKD AEWARAGKVI LPLLQNMLSD PYFSQPAPKS TGREYFNYGW
260 270 280 290 300
LERHLRHFPG VDPRDVQATL AELTAVTISE QVLLSGGCER LMVCGGGSRN
310 320 330 340 350
PLLMARLAAL LPGTEVTTTD AVGISGDDME ALAFAWLAWR TLAGLPGNLP
360
SVTGASQETV LGAIFPANP
Length:369
Mass (Da):39,496
Last modified:February 1, 1997 - v1
Checksum:iE0B439314E60F51B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74712.1.
AP009048 Genomic DNA. Translation: BAA15401.1.
PIRiB64921.
RefSeqiNP_416157.1. NC_000913.3.
WP_000835039.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74712; AAC74712; b1640.
BAA15401; BAA15401; BAA15401.
GeneIDi946810.
KEGGiecj:JW1632.
eco:b1640.
PATRICi32118582. VBIEscCol129921_1711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74712.1.
AP009048 Genomic DNA. Translation: BAA15401.1.
PIRiB64921.
RefSeqiNP_416157.1. NC_000913.3.
WP_000835039.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77570.
SMRiP77570. Positions 5-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263491. 6 interactions.
IntActiP77570. 7 interactions.
STRINGi511145.b1640.

Proteomic databases

PaxDbiP77570.
PRIDEiP77570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74712; AAC74712; b1640.
BAA15401; BAA15401; BAA15401.
GeneIDi946810.
KEGGiecj:JW1632.
eco:b1640.
PATRICi32118582. VBIEscCol129921_1711.

Organism-specific databases

EchoBASEiEB3700.
EcoGeneiEG13942. anmK.

Phylogenomic databases

eggNOGiENOG4105ED7. Bacteria.
COG2377. LUCA.
HOGENOMiHOG000256309.
InParanoidiP77570.
KOiK09001.
OMAiGCHGQTI.
PhylomeDBiP77570.

Enzyme and pathway databases

UniPathwayiUPA00343.
UPA00544.
BioCyciEcoCyc:G6880-MONOMER.
ECOL316407:JW1632-MONOMER.
MetaCyc:G6880-MONOMER.
SABIO-RKP77570.

Miscellaneous databases

PROiP77570.

Family and domain databases

HAMAPiMF_01270. AnhMurNAc_kinase. 1 hit.
InterProiIPR005338. Anhydro_N_Ac-Mur_kinase.
[Graphical view]
PfamiPF03702. UPF0075. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANMK_ECOLI
AccessioniPrimary (citable) accession number: P77570
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.