Reviewed, UniProtKB/Swiss-Prot P77555 (ALLD_ECOLI)

Names and origin

Protein names

Recommended name:
    Ureidoglycolate dehydrogenase
    EC=1.1.1.154

Gene names

Name:	allD
Synonyms:	glxB8, ylbC
Ordered Locus Names:	b0517, JW0505

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the anaerobic utilization of allantoin. Ref.1
Catalytic activity	(S)-ureidoglycolate + NAD(P)+ = oxalureate + NAD(P)H.
Pathway	Nitrogen metabolism; (S)-allantoin degradation; oxalureate from (S)-ureidoglycolate: step 1/1.
Subcellular location	Cytoplasm Probable.
Induction	By glyoxylate and allantoin under anaerobic conditions.
Sequence similarities	Belongs to the LDH2/MDH2 oxidoreductase family.

Ontologies

Keywords
Biological process	Purine metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ureidoglycolate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 349

349

Ureidoglycolate dehydrogenase

PRO_0000083822

Secondary structure

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349

Helix Strand Turn

Details...

Helix

5 – 19

15

Helix

23 – 38

16

Helix

42 – 44

3

Helix

46 – 48

3

Helix

49 – 57

9

Beta strand

68 – 71

4

Beta strand

73 – 84

12

Helix

86 – 104

19

Beta strand

105 – 114

10

Helix

121 – 129

9

Beta strand

132 – 138

7

Beta strand

149 – 151

3

Beta strand

159 – 164

6

Beta strand

170 – 175

6

Beta strand

177 – 180

4

Helix

182 – 189

8

Turn

190 – 192

3

Beta strand

199 – 201

3

Turn

211 – 213

3

Turn

220 – 222

3

Helix

223 – 236

14

Helix

238 – 240

3

Helix

245 – 247

3

Turn

251 – 253

3

Beta strand

261 – 268

8

Helix

270 – 272

3

Helix

276 – 291

16

Helix

307 – 319

13

Beta strand

321 – 323

3

Helix

325 – 332

8

Sequences

	Sequence		Length	Mass (Da)
	P77555-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 93C3076A66141C4E	FASTA	349	37,967
	10 20 30 40 50 60 MKISRETLHQ LIENKLCQAG LKREHAATVA EVLVYADARG IHSHGAVRVE YYAERISKGG 70 80 90 100 110 120 TNREPEFRLE ETGPCSAILH ADNAAGQVAA KMGMEHAIKT AQQNGVAVVG ISRMGHSGAI 130 140 150 160 170 180 SYFVQQAARA GFIGISMCQS DPMVVPFGGA EIYYGTNPLA FAAPGEGDEI LTFDMATTVQ 190 200 210 220 230 240 AWGKVLDARS RNMSIPDTWA VDKNGVPTTD PFAVHALLPA AGPKGYGLMM MIDVLSGVLL 250 260 270 280 290 300 GLPFGRQVSS MYDDLHAGRN LGQLHIVINP NFFSSSELFR QHLSQTMREL NAITPAPGFN 310 320 330 340 QVYYPGQDQD IKQRKAAVEG IEIVDDIYQY LISDALYNTS YETKNPFAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli." Cusa E., Obradors N., Baldoma L., Badia J., Aguilar J. J. Bacteriol. 181:7479-7484(1999) [PubMed: 10601204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: K12 / ECL1.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U89279 Genomic DNA. Translation: AAB93858.1.
U82664 Genomic DNA. Translation: AAB40269.1.
U00096 Genomic DNA. Translation: AAC73619.1.
AP009048 Genomic DNA. Translation: BAE76295.1.

PIR

D64783.

RefSeq

AP_001165.1.
NP_415050.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XRH	X-ray	2.25	A/B/C/D/E/F/G/H	2-349	[»]

ModBase

Search...

Genome annotation databases

GeneID

948342.

GenomeReviews

Gene locus JW0505 in contig AP009048_GR.
Gene locus b0517 in contig U00096_GR.

KEGG

ecj:JW0505.
eco:b0517.

Organism-specific databases

EchoBASE

EB3389.

EcoGene

EG13624. allD.

CMR

Search...

Phylogenomic databases

HOGENOM

P77555.

OMA

P77555. GLMMMVD.

Enzyme and pathway databases

BioCyc

EcoCyc:G6286-MON.
MetaCyc:G6286-MON.

Family and domain databases

InterPro

IPR003767. Malate/L-lactate_DH.
IPR017590. Ureidoglycolate_dehydrogenase.
[Graphical view]

PANTHER

PTHR11091. MDH_LDH. 1 hit.

Pfam

PF02615. Ldh_2. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03175. AllD. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ALLD_ECOLI

Accession

Primary (citable) accession number: P77555
Secondary accession number(s): Q2MBR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)