Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione S-transferase YfcF

Gene

yfcF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits glutathione (GSH) S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB); however this activity is as low as 1% of that of GstA. Also displays a GSH-dependent peroxidase activity toward cumene hydroperoxide. Is involved in defense against oxidative stress, probably via its peroxidase activity.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Enzyme regulationi

GSH transferase activity is inhibited by S-hexyl glutathione.1 Publication

Kineticsi

kcat is 0.11 sec(-1) for the GSH transferase reaction with CDNB as substrate.

  1. KM=0.24 mM for glutathione1 Publication
  2. KM=1.1 mM for 1-chloro-2,4-dinitrobenzene1 Publication

    pH dependencei

    Activity decreases as pH is lowered from neutral to acidic.1 Publication

    GO - Molecular functioni

    • glutathione transferase activity Source: EcoCyc
    • peroxidase activity Source: UniProtKB-KW

    GO - Biological processi

    • glutathione metabolic process Source: GO_Central
    • response to hydrogen peroxide Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase, Transferase

    Enzyme and pathway databases

    BioCyciEcoCyc:G7193-MONOMER.
    ECOL316407:JW2298-MONOMER.
    MetaCyc:G7193-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase YfcF (EC:2.5.1.18)
    Gene namesi
    Name:yfcF
    Ordered Locus Names:b2301, JW2298
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG14109. yfcF.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    Deletion of yfcF decreases the resistance of the bacteria to hydrogen peroxide.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi10S → A: Nearly no effect on GSH transferase activity. 1 Publication1
    Mutagenesisi16S → G: 2% of wild-type GSH transferase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001860151 – 214Glutathione S-transferase YfcFAdd BLAST214

    Proteomic databases

    PaxDbiP77544.
    PRIDEiP77544.

    Interactioni

    Protein-protein interaction databases

    BioGridi4260516. 12 interactors.
    IntActiP77544. 3 interactors.
    STRINGi511145.b2301.

    Structurei

    Secondary structure

    1214
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi6 – 11Combined sources6
    Helixi17 – 29Combined sources13
    Beta strandi34 – 38Combined sources5
    Beta strandi61 – 64Combined sources4
    Beta strandi67 – 71Combined sources5
    Helixi72 – 82Combined sources11
    Turni85 – 87Combined sources3
    Helixi96 – 111Combined sources16
    Helixi114 – 119Combined sources6
    Helixi123 – 126Combined sources4
    Helixi136 – 152Combined sources17
    Turni153 – 155Combined sources3
    Helixi166 – 179Combined sources14
    Turni180 – 182Combined sources3
    Helixi187 – 197Combined sources11
    Helixi200 – 210Combined sources11

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3BBYX-ray1.85A1-214[»]
    ProteinModelPortaliP77544.
    SMRiP77544.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP77544.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini6 – 87GST N-terminalAdd BLAST82
    Domaini95 – 214GST C-terminalAdd BLAST120

    Sequence similaritiesi

    Belongs to the GST superfamily.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiENOG4108SHQ. Bacteria.
    COG0625. LUCA.
    HOGENOMiHOG000255229.
    InParanoidiP77544.
    KOiK00799.
    OMAiFSPYVMS.
    PhylomeDBiP77544.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P77544-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKPAITLWS DAHFFSPYVL SAWVALQEKG LSFHIKTIDL DSGEHLQPTW
    60 70 80 90 100
    QGYGQTRRVP LLQIDDFELS ESSAIAEYLE DRFAPPTWER IYPLDLENRA
    110 120 130 140 150
    RARQIQAWLR SDLMPIREER PTDVVFAGAK KAPLTAEGKA SAEKLFAMAE
    160 170 180 190 200
    HLLVLGQPNL FGEWCIADTD LALMINRLVL HGDEVPERLV DYATFQWQRA
    210
    SVQRFIALSA KQSG
    Length:214
    Mass (Da):24,326
    Last modified:February 1, 1997 - v1
    Checksum:i6B2308D403DA3077
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75361.1.
    AP009048 Genomic DNA. Translation: BAA16138.1.
    PIRiC65002.
    RefSeqiNP_416804.1. NC_000913.3.
    WP_000042442.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75361; AAC75361; b2301.
    BAA16138; BAA16138; BAA16138.
    GeneIDi946749.
    KEGGiecj:JW2298.
    eco:b2301.
    PATRICi32119973. VBIEscCol129921_2396.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75361.1.
    AP009048 Genomic DNA. Translation: BAA16138.1.
    PIRiC65002.
    RefSeqiNP_416804.1. NC_000913.3.
    WP_000042442.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3BBYX-ray1.85A1-214[»]
    ProteinModelPortaliP77544.
    SMRiP77544.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260516. 12 interactors.
    IntActiP77544. 3 interactors.
    STRINGi511145.b2301.

    Proteomic databases

    PaxDbiP77544.
    PRIDEiP77544.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75361; AAC75361; b2301.
    BAA16138; BAA16138; BAA16138.
    GeneIDi946749.
    KEGGiecj:JW2298.
    eco:b2301.
    PATRICi32119973. VBIEscCol129921_2396.

    Organism-specific databases

    EchoBASEiEB3862.
    EcoGeneiEG14109. yfcF.

    Phylogenomic databases

    eggNOGiENOG4108SHQ. Bacteria.
    COG0625. LUCA.
    HOGENOMiHOG000255229.
    InParanoidiP77544.
    KOiK00799.
    OMAiFSPYVMS.
    PhylomeDBiP77544.

    Enzyme and pathway databases

    BioCyciEcoCyc:G7193-MONOMER.
    ECOL316407:JW2298-MONOMER.
    MetaCyc:G7193-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP77544.
    PROiP77544.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiYFCF_ECOLI
    AccessioniPrimary (citable) accession number: P77544
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: November 2, 2016
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.