Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione S-transferase YfcF

Gene

yfcF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits glutathione (GSH) S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB); however this activity is as low as 1% of that of GstA. Also displays a GSH-dependent peroxidase activity toward cumene hydroperoxide. Is involved in defense against oxidative stress, probably via its peroxidase activity.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Enzyme regulationi

GSH transferase activity is inhibited by S-hexyl glutathione.1 Publication

Kineticsi

kcat is 0.11 sec(-1) for the GSH transferase reaction with CDNB as substrate.
  1. KM=0.24 mM for glutathione1 Publication
  2. KM=1.1 mM for 1-chloro-2,4-dinitrobenzene1 Publication

    pH dependencei

    Activity decreases as pH is lowered from neutral to acidic.1 Publication

    GO - Molecular functioni

    • glutathione transferase activity Source: EcoCyc
    • peroxidase activity Source: UniProtKB-KW

    GO - Biological processi

    • response to hydrogen peroxide Source: EcoCyc

    Keywordsi

    Molecular functionOxidoreductase, Peroxidase, Transferase

    Enzyme and pathway databases

    BioCyciEcoCyc:G7193-MONOMER
    MetaCyc:G7193-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase YfcF (EC:2.5.1.18)
    Gene namesi
    Name:yfcF
    Ordered Locus Names:b2301, JW2298
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG14109 yfcF

    Pathology & Biotechi

    Disruption phenotypei

    Deletion of yfcF decreases the resistance of the bacteria to hydrogen peroxide.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi10S → A: Nearly no effect on GSH transferase activity. 1 Publication1
    Mutagenesisi16S → G: 2% of wild-type GSH transferase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001860151 – 214Glutathione S-transferase YfcFAdd BLAST214

    Proteomic databases

    PaxDbiP77544
    PRIDEiP77544

    Interactioni

    Protein-protein interaction databases

    BioGridi4260516, 12 interactors
    IntActiP77544, 3 interactors
    STRINGi316385.ECDH10B_2463

    Structurei

    Secondary structure

    1214
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi6 – 11Combined sources6
    Helixi17 – 29Combined sources13
    Beta strandi34 – 38Combined sources5
    Beta strandi61 – 64Combined sources4
    Beta strandi67 – 71Combined sources5
    Helixi72 – 82Combined sources11
    Turni85 – 87Combined sources3
    Helixi96 – 111Combined sources16
    Helixi114 – 119Combined sources6
    Helixi123 – 126Combined sources4
    Helixi136 – 152Combined sources17
    Turni153 – 155Combined sources3
    Helixi166 – 179Combined sources14
    Turni180 – 182Combined sources3
    Helixi187 – 197Combined sources11
    Helixi200 – 210Combined sources11

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3BBYX-ray1.85A1-214[»]
    ProteinModelPortaliP77544
    SMRiP77544
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP77544

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini6 – 87GST N-terminalAdd BLAST82
    Domaini95 – 214GST C-terminalAdd BLAST120

    Sequence similaritiesi

    Belongs to the GST superfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4108SHQ Bacteria
    COG0625 LUCA
    HOGENOMiHOG000255229
    InParanoidiP77544
    KOiK00799
    OMAiFSPYVMS
    PhylomeDBiP77544

    Family and domain databases

    CDDicd03195 GST_C_4, 1 hit
    InterProiView protein in InterPro
    IPR010987 Glutathione-S-Trfase_C-like
    IPR036282 Glutathione-S-Trfase_C_sf
    IPR004045 Glutathione_S-Trfase_N
    IPR034338 GST_4_C
    IPR034339 GST_YfcF-like
    IPR036249 Thioredoxin-like_sf
    PANTHERiPTHR42673:SF6 PTHR42673:SF6, 1 hit
    PfamiView protein in Pfam
    PF14834 GST_C_4, 1 hit
    PF13417 GST_N_3, 1 hit
    SUPFAMiSSF47616 SSF47616, 1 hit
    SSF52833 SSF52833, 1 hit
    PROSITEiView protein in PROSITE
    PS50405 GST_CTER, 1 hit
    PS50404 GST_NTER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P77544-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKPAITLWS DAHFFSPYVL SAWVALQEKG LSFHIKTIDL DSGEHLQPTW
    60 70 80 90 100
    QGYGQTRRVP LLQIDDFELS ESSAIAEYLE DRFAPPTWER IYPLDLENRA
    110 120 130 140 150
    RARQIQAWLR SDLMPIREER PTDVVFAGAK KAPLTAEGKA SAEKLFAMAE
    160 170 180 190 200
    HLLVLGQPNL FGEWCIADTD LALMINRLVL HGDEVPERLV DYATFQWQRA
    210
    SVQRFIALSA KQSG
    Length:214
    Mass (Da):24,326
    Last modified:February 1, 1997 - v1
    Checksum:i6B2308D403DA3077
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC75361.1
    AP009048 Genomic DNA Translation: BAA16138.1
    PIRiC65002
    RefSeqiNP_416804.1, NC_000913.3
    WP_000042442.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC75361; AAC75361; b2301
    BAA16138; BAA16138; BAA16138
    GeneIDi946749
    KEGGiecj:JW2298
    eco:b2301
    PATRICifig|1411691.4.peg.4433

    Similar proteinsi

    Entry informationi

    Entry nameiYFCF_ECOLI
    AccessioniPrimary (citable) accession number: P77544
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: March 28, 2018
    This is version 118 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health