ID   YKGF_ECOLI              Reviewed;         475 AA.
AC   P77536; Q2MCB5;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   24-JAN-2024, entry version 162.
DE   RecName: Full=Uncharacterized electron transport protein YkgF;
GN   Name=ykgF; OrderedLocusNames=b0307, JW0300;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   LACK OF COMPLEMENTATION OF B.SUBTILIS LUTABC OPERON.
RC   STRAIN=K12;
RX   PubMed=19201793; DOI=10.1128/jb.01464-08;
RA   Chai Y., Kolter R., Losick R.;
RT   "A widely conserved gene cluster required for lactate utilization in
RT   Bacillus subtilis and its involvement in biofilm formation.";
RL   J. Bacteriol. 191:2423-2430(2009).
CC   -!- MISCELLANEOUS: The E.coli ykgEFG operon is a clear homolog of the
CC       B.subtilis lutABC operon, however it is not able of restoring L-lactate
CC       utilization to the B.subtilis mutant for lutABC operon. Thus, ykgEFG
CC       operon may contribute to lactate catabolism in E.coli, but under
CC       conditions other than those tested, or alternatively, the ykgEFG operon
CC       may be responsible for the catabolism of a metabolite other than (but
CC       perhaps related to) lactate in E.coli.
CC   -!- SIMILARITY: Belongs to the LutB/YkgF family. {ECO:0000305}.
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DR   EMBL; U73857; AAB18034.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73410.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76091.1; -; Genomic_DNA.
DR   PIR; C64757; C64757.
DR   RefSeq; NP_414841.1; NC_000913.3.
DR   RefSeq; WP_000023927.1; NZ_STEB01000020.1.
DR   AlphaFoldDB; P77536; -.
DR   BioGRID; 4260872; 6.
DR   IntAct; P77536; 26.
DR   STRING; 511145.b0307; -.
DR   jPOST; P77536; -.
DR   PaxDb; 511145-b0307; -.
DR   EnsemblBacteria; AAC73410; AAC73410; b0307.
DR   GeneID; 944980; -.
DR   KEGG; ecj:JW0300; -.
DR   KEGG; eco:b0307; -.
DR   PATRIC; fig|1411691.4.peg.1970; -.
DR   EchoBASE; EB3353; -.
DR   eggNOG; COG1139; Bacteria.
DR   HOGENOM; CLU_027059_2_0_6; -.
DR   InParanoid; P77536; -.
DR   OMA; HCPVYDK; -.
DR   OrthoDB; 5289041at2; -.
DR   PhylomeDB; P77536; -.
DR   BioCyc; EcoCyc:G6177-MONOMER; -.
DR   PRO; PR:P77536; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019516; P:lactate oxidation; IEA:InterPro.
DR   Gene3D; 1.10.1060.10; Alpha-helical ferredoxin; 1.
DR   Gene3D; 3.40.50.10420; NagB/RpiA/CoA transferase-like; 1.
DR   InterPro; IPR004452; 4Fe-4S-bd.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR024185; FTHF_cligase-like_sf.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   InterPro; IPR003741; LUD_dom.
DR   InterPro; IPR024569; LutB_C.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   NCBIfam; TIGR00273; LutB/LldF family L-lactate oxidation iron-sulfur protein; 1.
DR   PANTHER; PTHR47153; LACTATE UTILIZATION PROTEIN B; 1.
DR   PANTHER; PTHR47153:SF2; LACTATE UTILIZATION PROTEIN B; 1.
DR   Pfam; PF13183; Fer4_8; 1.
DR   Pfam; PF02589; LUD_dom; 1.
DR   Pfam; PF11870; LutB_C; 1.
DR   SUPFAM; SSF46548; alpha-helical ferredoxin; 1.
DR   SUPFAM; SSF100950; NagB/RpiA/CoA transferase-like; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 2.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
PE   3: Inferred from homology;
KW   4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding;
KW   Reference proteome; Repeat; Transport.
FT   CHAIN           1..475
FT                   /note="Uncharacterized electron transport protein YkgF"
FT                   /id="PRO_0000159302"
FT   DOMAIN          303..333
FT                   /note="4Fe-4S ferredoxin-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT   DOMAIN          352..381
FT                   /note="4Fe-4S ferredoxin-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT   BINDING         312
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         315
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         318
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         322
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         362
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         365
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         368
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         372
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   475 AA;  53052 MW;  6B2429D387336F72 CRC64;
     MSIKTSNTDF KTRIRQQIED PIMRKAVANA QQRIGANRQK MVDELGHWEE WRDRAAQIRD
     HVLSNLDAYL YQLSEKVTQN GGHVYFARTK EDATRYILQV AQRKNARKVV KSKSMVTEEI
     GVNHVLQDAG IQVIETDLGE YILQLDQDPP SHVVVPAIHK DRHQIRRVLH ERLGYEGPET
     PEAMTLFIRQ KIREDFLSAE IGITGCNFAV AETGSVCLVT NEGNARMCTT LPKTHIAVMG
     MERIAPTFAE VDVLITMLAR SAVGARLTGY NTWLTGPREA GHVDGPEEFH LVIVDNGRSE
     VLASEFRDVL RCIRCGACMN TCPAYRHIGG HGYGSIYPGP IGAVISPLLG GYKDFKDLPY
     ACSLCTACDN VCPVRIPLSK LILRHRRVMA EKGITAKAEQ RAIKMFAYAN SHPGLWKVGM
     MAGAHAASWF INGGKTPLKF GAISDWMEAR DLPEADGESF RSWFKKHQAQ EKKNG
//