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P77536

- YKGF_ECOLI

UniProt

P77536 - YKGF_ECOLI

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Protein
Uncharacterized electron transport protein YkgF
Gene
ykgF, b0307, JW0300
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi312 – 3121Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi315 – 3151Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi318 – 3181Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi322 – 3221Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi362 – 3621Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi365 – 3651Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi368 – 3681Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi372 – 3721Iron-sulfur 1 (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. lactate oxidation Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6177-MONOMER.
ECOL316407:JW0300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized electron transport protein YkgF
Gene namesi
Name:ykgF
Ordered Locus Names:b0307, JW0300
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13583. ykgF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Uncharacterized electron transport protein YkgF
PRO_0000159302Add
BLAST

Proteomic databases

PaxDbiP77536.
PRIDEiP77536.

Expressioni

Gene expression databases

GenevestigatoriP77536.

Interactioni

Protein-protein interaction databases

IntActiP77536. 26 interactions.
STRINGi511145.b0307.

Structurei

3D structure databases

ProteinModelPortaliP77536.
SMRiP77536. Positions 309-376.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini303 – 333314Fe-4S ferredoxin-type 1
Add
BLAST
Domaini352 – 381304Fe-4S ferredoxin-type 2
Add
BLAST

Sequence similaritiesi

Belongs to the LutB/YkgF family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1139.
HOGENOMiHOG000262102.
OMAiAIHLKKE.
OrthoDBiEOG6JTC5N.
PhylomeDBiP77536.

Family and domain databases

Gene3Di3.40.50.10420. 2 hits.
InterProiIPR004452. 4Fe-4S-bd.
IPR024569. 4Fe-4S-bd_C.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR003741. DUF162.
IPR024185. FTHF_cligase-like.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamiPF02589. DUF162. 1 hit.
PF11870. DUF3390. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR00273. TIGR00273. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77536-1 [UniParc]FASTAAdd to Basket

« Hide

MSIKTSNTDF KTRIRQQIED PIMRKAVANA QQRIGANRQK MVDELGHWEE    50
WRDRAAQIRD HVLSNLDAYL YQLSEKVTQN GGHVYFARTK EDATRYILQV 100
AQRKNARKVV KSKSMVTEEI GVNHVLQDAG IQVIETDLGE YILQLDQDPP 150
SHVVVPAIHK DRHQIRRVLH ERLGYEGPET PEAMTLFIRQ KIREDFLSAE 200
IGITGCNFAV AETGSVCLVT NEGNARMCTT LPKTHIAVMG MERIAPTFAE 250
VDVLITMLAR SAVGARLTGY NTWLTGPREA GHVDGPEEFH LVIVDNGRSE 300
VLASEFRDVL RCIRCGACMN TCPAYRHIGG HGYGSIYPGP IGAVISPLLG 350
GYKDFKDLPY ACSLCTACDN VCPVRIPLSK LILRHRRVMA EKGITAKAEQ 400
RAIKMFAYAN SHPGLWKVGM MAGAHAASWF INGGKTPLKF GAISDWMEAR 450
DLPEADGESF RSWFKKHQAQ EKKNG 475
Length:475
Mass (Da):53,052
Last modified:February 1, 1997 - v1
Checksum:i6B2429D387336F72
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73857 Genomic DNA. Translation: AAB18034.1.
U00096 Genomic DNA. Translation: AAC73410.1.
AP009048 Genomic DNA. Translation: BAE76091.1.
PIRiC64757.
RefSeqiNP_414841.1. NC_000913.3.
YP_488603.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73410; AAC73410; b0307.
BAE76091; BAE76091; BAE76091.
GeneIDi12931878.
944980.
KEGGiecj:Y75_p0298.
eco:b0307.
PATRICi32115745. VBIEscCol129921_0315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73857 Genomic DNA. Translation: AAB18034.1 .
U00096 Genomic DNA. Translation: AAC73410.1 .
AP009048 Genomic DNA. Translation: BAE76091.1 .
PIRi C64757.
RefSeqi NP_414841.1. NC_000913.3.
YP_488603.1. NC_007779.1.

3D structure databases

ProteinModelPortali P77536.
SMRi P77536. Positions 309-376.
ModBasei Search...

Protein-protein interaction databases

IntActi P77536. 26 interactions.
STRINGi 511145.b0307.

Proteomic databases

PaxDbi P77536.
PRIDEi P77536.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73410 ; AAC73410 ; b0307 .
BAE76091 ; BAE76091 ; BAE76091 .
GeneIDi 12931878.
944980.
KEGGi ecj:Y75_p0298.
eco:b0307.
PATRICi 32115745. VBIEscCol129921_0315.

Organism-specific databases

EchoBASEi EB3353.
EcoGenei EG13583. ykgF.

Phylogenomic databases

eggNOGi COG1139.
HOGENOMi HOG000262102.
OMAi AIHLKKE.
OrthoDBi EOG6JTC5N.
PhylomeDBi P77536.

Enzyme and pathway databases

BioCyci EcoCyc:G6177-MONOMER.
ECOL316407:JW0300-MONOMER.

Miscellaneous databases

PROi P77536.

Gene expression databases

Genevestigatori P77536.

Family and domain databases

Gene3Di 3.40.50.10420. 2 hits.
InterProi IPR004452. 4Fe-4S-bd.
IPR024569. 4Fe-4S-bd_C.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR003741. DUF162.
IPR024185. FTHF_cligase-like.
IPR009051. Helical_ferredxn.
[Graphical view ]
Pfami PF02589. DUF162. 1 hit.
PF11870. DUF3390. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view ]
SUPFAMi SSF46548. SSF46548. 1 hit.
TIGRFAMsi TIGR00273. TIGR00273. 1 hit.
PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "A widely conserved gene cluster required for lactate utilization in Bacillus subtilis and its involvement in biofilm formation."
    Chai Y., Kolter R., Losick R.
    J. Bacteriol. 191:2423-2430(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF COMPLEMENTATION OF B.SUBTILIS LUTABC OPERON.
    Strain: K12.

Entry informationi

Entry nameiYKGF_ECOLI
AccessioniPrimary (citable) accession number: P77536
Secondary accession number(s): Q2MCB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The E.coli ykgEFG operon is a clear homolog of the B.subtilis lutABC operon, however it is not able of restoring L-lactate utilization to the B.subtilis mutant for lutABC operon. Thus, ykgEFG operon may contribute to lactate catabolism in E.coli, but under conditions other than those tested, or alternatively, the ykgEFG operon may be responsible for the catabolism of a metabolite other than (but perhaps related to) lactate in E.coli.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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