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P77536

- YKGF_ECOLI

UniProt

P77536 - YKGF_ECOLI

Protein

Uncharacterized electron transport protein YkgF

Gene

ykgF

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi312 – 3121Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi315 – 3151Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi318 – 3181Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi322 – 3221Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi362 – 3621Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi365 – 3651Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi368 – 3681Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi372 – 3721Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. lactate oxidation Source: InterPro

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G6177-MONOMER.
    ECOL316407:JW0300-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uncharacterized electron transport protein YkgF
    Gene namesi
    Name:ykgF
    Ordered Locus Names:b0307, JW0300
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13583. ykgF.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 475475Uncharacterized electron transport protein YkgFPRO_0000159302Add
    BLAST

    Proteomic databases

    PaxDbiP77536.
    PRIDEiP77536.

    Expressioni

    Gene expression databases

    GenevestigatoriP77536.

    Interactioni

    Protein-protein interaction databases

    IntActiP77536. 26 interactions.
    STRINGi511145.b0307.

    Structurei

    3D structure databases

    ProteinModelPortaliP77536.
    SMRiP77536. Positions 309-376.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini303 – 333314Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini352 – 381304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the LutB/YkgF family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1139.
    HOGENOMiHOG000262102.
    OMAiAIHLKKE.
    OrthoDBiEOG6JTC5N.
    PhylomeDBiP77536.

    Family and domain databases

    Gene3Di3.40.50.10420. 2 hits.
    InterProiIPR004452. 4Fe-4S-bd.
    IPR024569. 4Fe-4S-bd_C.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR003741. DUF162.
    IPR024185. FTHF_cligase-like.
    IPR009051. Helical_ferredxn.
    [Graphical view]
    PfamiPF02589. DUF162. 1 hit.
    PF11870. DUF3390. 1 hit.
    PF13183. Fer4_8. 1 hit.
    [Graphical view]
    SUPFAMiSSF46548. SSF46548. 1 hit.
    TIGRFAMsiTIGR00273. TIGR00273. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P77536-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIKTSNTDF KTRIRQQIED PIMRKAVANA QQRIGANRQK MVDELGHWEE    50
    WRDRAAQIRD HVLSNLDAYL YQLSEKVTQN GGHVYFARTK EDATRYILQV 100
    AQRKNARKVV KSKSMVTEEI GVNHVLQDAG IQVIETDLGE YILQLDQDPP 150
    SHVVVPAIHK DRHQIRRVLH ERLGYEGPET PEAMTLFIRQ KIREDFLSAE 200
    IGITGCNFAV AETGSVCLVT NEGNARMCTT LPKTHIAVMG MERIAPTFAE 250
    VDVLITMLAR SAVGARLTGY NTWLTGPREA GHVDGPEEFH LVIVDNGRSE 300
    VLASEFRDVL RCIRCGACMN TCPAYRHIGG HGYGSIYPGP IGAVISPLLG 350
    GYKDFKDLPY ACSLCTACDN VCPVRIPLSK LILRHRRVMA EKGITAKAEQ 400
    RAIKMFAYAN SHPGLWKVGM MAGAHAASWF INGGKTPLKF GAISDWMEAR 450
    DLPEADGESF RSWFKKHQAQ EKKNG 475
    Length:475
    Mass (Da):53,052
    Last modified:February 1, 1997 - v1
    Checksum:i6B2429D387336F72
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73857 Genomic DNA. Translation: AAB18034.1.
    U00096 Genomic DNA. Translation: AAC73410.1.
    AP009048 Genomic DNA. Translation: BAE76091.1.
    PIRiC64757.
    RefSeqiNP_414841.1. NC_000913.3.
    YP_488603.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73410; AAC73410; b0307.
    BAE76091; BAE76091; BAE76091.
    GeneIDi12931878.
    944980.
    KEGGiecj:Y75_p0298.
    eco:b0307.
    PATRICi32115745. VBIEscCol129921_0315.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73857 Genomic DNA. Translation: AAB18034.1 .
    U00096 Genomic DNA. Translation: AAC73410.1 .
    AP009048 Genomic DNA. Translation: BAE76091.1 .
    PIRi C64757.
    RefSeqi NP_414841.1. NC_000913.3.
    YP_488603.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P77536.
    SMRi P77536. Positions 309-376.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P77536. 26 interactions.
    STRINGi 511145.b0307.

    Proteomic databases

    PaxDbi P77536.
    PRIDEi P77536.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73410 ; AAC73410 ; b0307 .
    BAE76091 ; BAE76091 ; BAE76091 .
    GeneIDi 12931878.
    944980.
    KEGGi ecj:Y75_p0298.
    eco:b0307.
    PATRICi 32115745. VBIEscCol129921_0315.

    Organism-specific databases

    EchoBASEi EB3353.
    EcoGenei EG13583. ykgF.

    Phylogenomic databases

    eggNOGi COG1139.
    HOGENOMi HOG000262102.
    OMAi AIHLKKE.
    OrthoDBi EOG6JTC5N.
    PhylomeDBi P77536.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6177-MONOMER.
    ECOL316407:JW0300-MONOMER.

    Miscellaneous databases

    PROi P77536.

    Gene expression databases

    Genevestigatori P77536.

    Family and domain databases

    Gene3Di 3.40.50.10420. 2 hits.
    InterProi IPR004452. 4Fe-4S-bd.
    IPR024569. 4Fe-4S-bd_C.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR003741. DUF162.
    IPR024185. FTHF_cligase-like.
    IPR009051. Helical_ferredxn.
    [Graphical view ]
    Pfami PF02589. DUF162. 1 hit.
    PF11870. DUF3390. 1 hit.
    PF13183. Fer4_8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46548. SSF46548. 1 hit.
    TIGRFAMsi TIGR00273. TIGR00273. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "A widely conserved gene cluster required for lactate utilization in Bacillus subtilis and its involvement in biofilm formation."
      Chai Y., Kolter R., Losick R.
      J. Bacteriol. 191:2423-2430(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: LACK OF COMPLEMENTATION OF B.SUBTILIS LUTABC OPERON.
      Strain: K12.

    Entry informationi

    Entry nameiYKGF_ECOLI
    AccessioniPrimary (citable) accession number: P77536
    Secondary accession number(s): Q2MCB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The E.coli ykgEFG operon is a clear homolog of the B.subtilis lutABC operon, however it is not able of restoring L-lactate utilization to the B.subtilis mutant for lutABC operon. Thus, ykgEFG operon may contribute to lactate catabolism in E.coli, but under conditions other than those tested, or alternatively, the ykgEFG operon may be responsible for the catabolism of a metabolite other than (but perhaps related to) lactate in E.coli.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3