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P77536 (YKGF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uncharacterized electron transport protein YkgF
Gene names
Name:ykgF
Ordered Locus Names:b0307, JW0300
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Miscellaneous

The E.coli ykgEFG operon is a clear homolog of the B.subtilis lutABC operon, however it is not able of restoring L-lactate utilization to the B.subtilis mutant for lutABC operon. Thus, ykgEFG operon may contribute to lactate catabolism in E.coli, but under conditions other than those tested, or alternatively, the ykgEFG operon may be responsible for the catabolism of a metabolite other than (but perhaps related to) lactate in E.coli.

Sequence similarities

Belongs to the LutB/YkgF family.

Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
   Biological processElectron transport
Transport
   DomainRepeat
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlactate oxidation

Inferred from electronic annotation. Source: InterPro

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 475475Uncharacterized electron transport protein YkgF
PRO_0000159302

Regions

Domain303 – 333314Fe-4S ferredoxin-type 1
Domain352 – 381304Fe-4S ferredoxin-type 2

Sites

Metal binding3121Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding3151Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding3181Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding3221Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding3621Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding3651Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding3681Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding3721Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P77536 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 6B2429D387336F72

FASTA47553,052
        10         20         30         40         50         60 
MSIKTSNTDF KTRIRQQIED PIMRKAVANA QQRIGANRQK MVDELGHWEE WRDRAAQIRD 

        70         80         90        100        110        120 
HVLSNLDAYL YQLSEKVTQN GGHVYFARTK EDATRYILQV AQRKNARKVV KSKSMVTEEI 

       130        140        150        160        170        180 
GVNHVLQDAG IQVIETDLGE YILQLDQDPP SHVVVPAIHK DRHQIRRVLH ERLGYEGPET 

       190        200        210        220        230        240 
PEAMTLFIRQ KIREDFLSAE IGITGCNFAV AETGSVCLVT NEGNARMCTT LPKTHIAVMG 

       250        260        270        280        290        300 
MERIAPTFAE VDVLITMLAR SAVGARLTGY NTWLTGPREA GHVDGPEEFH LVIVDNGRSE 

       310        320        330        340        350        360 
VLASEFRDVL RCIRCGACMN TCPAYRHIGG HGYGSIYPGP IGAVISPLLG GYKDFKDLPY 

       370        380        390        400        410        420 
ACSLCTACDN VCPVRIPLSK LILRHRRVMA EKGITAKAEQ RAIKMFAYAN SHPGLWKVGM 

       430        440        450        460        470 
MAGAHAASWF INGGKTPLKF GAISDWMEAR DLPEADGESF RSWFKKHQAQ EKKNG 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"A widely conserved gene cluster required for lactate utilization in Bacillus subtilis and its involvement in biofilm formation."
Chai Y., Kolter R., Losick R.
J. Bacteriol. 191:2423-2430(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: LACK OF COMPLEMENTATION OF B.SUBTILIS LUTABC OPERON.
Strain: K12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U73857 Genomic DNA. Translation: AAB18034.1.
U00096 Genomic DNA. Translation: AAC73410.1.
AP009048 Genomic DNA. Translation: BAE76091.1.
PIRC64757.
RefSeqNP_414841.1. NC_000913.3.
YP_488603.1. NC_007779.1.

3D structure databases

ProteinModelPortalP77536.
SMRP77536. Positions 309-376.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP77536. 26 interactions.
STRING511145.b0307.

Proteomic databases

PaxDbP77536.
PRIDEP77536.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73410; AAC73410; b0307.
BAE76091; BAE76091; BAE76091.
GeneID12931878.
944980.
KEGGecj:Y75_p0298.
eco:b0307.
PATRIC32115745. VBIEscCol129921_0315.

Organism-specific databases

EchoBASEEB3353.
EcoGeneEG13583. ykgF.

Phylogenomic databases

eggNOGCOG1139.
HOGENOMHOG000262102.
OMAAIHLKKE.
OrthoDBEOG6JTC5N.
PhylomeDBP77536.

Enzyme and pathway databases

BioCycEcoCyc:G6177-MONOMER.
ECOL316407:JW0300-MONOMER.

Gene expression databases

GenevestigatorP77536.

Family and domain databases

Gene3D3.40.50.10420. 2 hits.
InterProIPR004452. 4Fe-4S-bd.
IPR024569. 4Fe-4S-bd_C.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR003741. DUF162.
IPR024185. FTHF_cligase-like.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamPF02589. DUF162. 1 hit.
PF11870. DUF3390. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMSSF46548. SSF46548. 1 hit.
TIGRFAMsTIGR00273. TIGR00273. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

PROP77536.

Entry information

Entry nameYKGF_ECOLI
AccessionPrimary (citable) accession number: P77536
Secondary accession number(s): Q2MCB5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene