P77536 (YKGF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized electron transport protein YkgF

Gene names

Name:	ykgF
Ordered Locus Names:	b0307, JW0300

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Miscellaneous

The E.coli ykgEFG operon is a clear homolog of the B.subtilis lutABC operon, however it is not able of restoring L-lactate utilization to the B.subtilis mutant for lutABC operon. Thus, ykgEFG operon may contribute to lactate catabolism in E.coli, but under conditions other than those tested, or alternatively, the ykgEFG operon may be responsible for the catabolism of a metabolite other than (but perhaps related to) lactate in E.coli.

Sequence similarities

Belongs to the LutB/YkgF family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW lactate oxidation Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Uncharacterized electron transport protein YkgF		PRO_0000159302
Regions
	Domain	303 – 333	31	4Fe-4S ferredoxin-type 1
	Domain	352 – 381	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	312	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	315	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	318	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	322	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	362	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	365	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	368	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	372	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77536 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 6B2429D387336F72	FASTA	475	53,052
	10 20 30 40 50 60 MSIKTSNTDF KTRIRQQIED PIMRKAVANA QQRIGANRQK MVDELGHWEE WRDRAAQIRD 70 80 90 100 110 120 HVLSNLDAYL YQLSEKVTQN GGHVYFARTK EDATRYILQV AQRKNARKVV KSKSMVTEEI 130 140 150 160 170 180 GVNHVLQDAG IQVIETDLGE YILQLDQDPP SHVVVPAIHK DRHQIRRVLH ERLGYEGPET 190 200 210 220 230 240 PEAMTLFIRQ KIREDFLSAE IGITGCNFAV AETGSVCLVT NEGNARMCTT LPKTHIAVMG 250 260 270 280 290 300 MERIAPTFAE VDVLITMLAR SAVGARLTGY NTWLTGPREA GHVDGPEEFH LVIVDNGRSE 310 320 330 340 350 360 VLASEFRDVL RCIRCGACMN TCPAYRHIGG HGYGSIYPGP IGAVISPLLG GYKDFKDLPY 370 380 390 400 410 420 ACSLCTACDN VCPVRIPLSK LILRHRRVMA EKGITAKAEQ RAIKMFAYAN SHPGLWKVGM 430 440 450 460 470 MAGAHAASWF INGGKTPLKF GAISDWMEAR DLPEADGESF RSWFKKHQAQ EKKNG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"A widely conserved gene cluster required for lactate utilization in Bacillus subtilis and its involvement in biofilm formation." Chai Y., Kolter R., Losick R. J. Bacteriol. 191:2423-2430(2009) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF COMPLEMENTATION OF B.SUBTILIS LUTABC OPERON. Strain: K12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73857 Genomic DNA. Translation: AAB18034.1. U00096 Genomic DNA. Translation: AAC73410.1. AP009048 Genomic DNA. Translation: BAE76091.1.
PIR	C64757.
RefSeq	NP_414841.1. NC_000913.2. YP_488603.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P77536.
SMR	P77536. Positions 309-376.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77536. 26 interactions.
STRING	511145.b0307.
Proteomic databases
PaxDb	P77536.
PRIDE	P77536.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73410; AAC73410; b0307. BAE76091; BAE76091; BAE76091.
GeneID	12931878. 944980.
KEGG	ecj:Y75_p0298. eco:b0307.
PATRIC	32115745. VBIEscCol129921_0315.
Organism-specific databases
EchoBASE	EB3353.
EcoGene	EG13583. ykgF.
Phylogenomic databases
eggNOG	COG1139.
HOGENOM	HOG000262102.
OMA	LETFEQN.
ProtClustDB	CLSK879631.
Enzyme and pathway databases
BioCyc	EcoCyc:G6177-MONOMER. ECOL316407:JW0300-MONOMER.
Gene expression databases
Genevestigator	P77536.
Family and domain databases
Gene3D	1.10.1060.10. 1 hit. 3.40.50.10420. 2 hits.
InterPro	IPR004452. 4Fe-4S-bd. IPR024569. 4Fe-4S-bd_C. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR003741. DUF162. IPR024185. FTHF_cligase-like. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. [Graphical view]
Pfam	PF02589. DUF162. 1 hit. PF11870. DUF3390. 1 hit. PF13183. Fer4_8. 1 hit. [Graphical view]
SUPFAM	SSF46548. Helical_ferredxn. 1 hit.
TIGRFAMs	TIGR00273. TIGR00273. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YKGF_ECOLI

Accession

Primary (citable) accession number: P77536
Secondary accession number(s): Q2MCB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program