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Protein

Uncharacterized electron transport protein YkgF

Gene

ykgF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi312 – 3121Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi315 – 3151Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi318 – 3181Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi322 – 3221Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi362 – 3621Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi365 – 3651Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi368 – 3681Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi372 – 3721Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. lactate oxidation Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6177-MONOMER.
ECOL316407:JW0300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized electron transport protein YkgF
Gene namesi
Name:ykgF
Ordered Locus Names:b0307, JW0300
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13583. ykgF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Uncharacterized electron transport protein YkgFPRO_0000159302Add
BLAST

Proteomic databases

PaxDbiP77536.
PRIDEiP77536.

Expressioni

Gene expression databases

GenevestigatoriP77536.

Interactioni

Protein-protein interaction databases

IntActiP77536. 26 interactions.
STRINGi511145.b0307.

Structurei

3D structure databases

ProteinModelPortaliP77536.
SMRiP77536. Positions 309-379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini303 – 333314Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini352 – 381304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the LutB/YkgF family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1139.
HOGENOMiHOG000262102.
InParanoidiP77536.
OMAiGTVHWAR.
OrthoDBiEOG6JTC5N.
PhylomeDBiP77536.

Family and domain databases

Gene3Di3.40.50.10420. 2 hits.
InterProiIPR004452. 4Fe-4S-bd.
IPR024569. 4Fe-4S-bd_C.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR003741. DUF162.
IPR024185. FTHF_cligase-like.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamiPF02589. DUF162. 1 hit.
PF11870. DUF3390. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR00273. TIGR00273. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77536-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIKTSNTDF KTRIRQQIED PIMRKAVANA QQRIGANRQK MVDELGHWEE
60 70 80 90 100
WRDRAAQIRD HVLSNLDAYL YQLSEKVTQN GGHVYFARTK EDATRYILQV
110 120 130 140 150
AQRKNARKVV KSKSMVTEEI GVNHVLQDAG IQVIETDLGE YILQLDQDPP
160 170 180 190 200
SHVVVPAIHK DRHQIRRVLH ERLGYEGPET PEAMTLFIRQ KIREDFLSAE
210 220 230 240 250
IGITGCNFAV AETGSVCLVT NEGNARMCTT LPKTHIAVMG MERIAPTFAE
260 270 280 290 300
VDVLITMLAR SAVGARLTGY NTWLTGPREA GHVDGPEEFH LVIVDNGRSE
310 320 330 340 350
VLASEFRDVL RCIRCGACMN TCPAYRHIGG HGYGSIYPGP IGAVISPLLG
360 370 380 390 400
GYKDFKDLPY ACSLCTACDN VCPVRIPLSK LILRHRRVMA EKGITAKAEQ
410 420 430 440 450
RAIKMFAYAN SHPGLWKVGM MAGAHAASWF INGGKTPLKF GAISDWMEAR
460 470
DLPEADGESF RSWFKKHQAQ EKKNG
Length:475
Mass (Da):53,052
Last modified:February 1, 1997 - v1
Checksum:i6B2429D387336F72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18034.1.
U00096 Genomic DNA. Translation: AAC73410.1.
AP009048 Genomic DNA. Translation: BAE76091.1.
PIRiC64757.
RefSeqiNP_414841.1. NC_000913.3.
YP_488603.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73410; AAC73410; b0307.
BAE76091; BAE76091; BAE76091.
GeneIDi12931878.
944980.
KEGGiecj:Y75_p0298.
eco:b0307.
PATRICi32115745. VBIEscCol129921_0315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18034.1.
U00096 Genomic DNA. Translation: AAC73410.1.
AP009048 Genomic DNA. Translation: BAE76091.1.
PIRiC64757.
RefSeqiNP_414841.1. NC_000913.3.
YP_488603.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP77536.
SMRiP77536. Positions 309-379.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP77536. 26 interactions.
STRINGi511145.b0307.

Proteomic databases

PaxDbiP77536.
PRIDEiP77536.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73410; AAC73410; b0307.
BAE76091; BAE76091; BAE76091.
GeneIDi12931878.
944980.
KEGGiecj:Y75_p0298.
eco:b0307.
PATRICi32115745. VBIEscCol129921_0315.

Organism-specific databases

EchoBASEiEB3353.
EcoGeneiEG13583. ykgF.

Phylogenomic databases

eggNOGiCOG1139.
HOGENOMiHOG000262102.
InParanoidiP77536.
OMAiGTVHWAR.
OrthoDBiEOG6JTC5N.
PhylomeDBiP77536.

Enzyme and pathway databases

BioCyciEcoCyc:G6177-MONOMER.
ECOL316407:JW0300-MONOMER.

Miscellaneous databases

PROiP77536.

Gene expression databases

GenevestigatoriP77536.

Family and domain databases

Gene3Di3.40.50.10420. 2 hits.
InterProiIPR004452. 4Fe-4S-bd.
IPR024569. 4Fe-4S-bd_C.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR003741. DUF162.
IPR024185. FTHF_cligase-like.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamiPF02589. DUF162. 1 hit.
PF11870. DUF3390. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR00273. TIGR00273. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "A widely conserved gene cluster required for lactate utilization in Bacillus subtilis and its involvement in biofilm formation."
    Chai Y., Kolter R., Losick R.
    J. Bacteriol. 191:2423-2430(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF COMPLEMENTATION OF B.SUBTILIS LUTABC OPERON.
    Strain: K12.

Entry informationi

Entry nameiYKGF_ECOLI
AccessioniPrimary (citable) accession number: P77536
Secondary accession number(s): Q2MCB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 4, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The E.coli ykgEFG operon is a clear homolog of the B.subtilis lutABC operon, however it is not able of restoring L-lactate utilization to the B.subtilis mutant for lutABC operon. Thus, ykgEFG operon may contribute to lactate catabolism in E.coli, but under conditions other than those tested, or alternatively, the ykgEFG operon may be responsible for the catabolism of a metabolite other than (but perhaps related to) lactate in E.coli.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.