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Protein

FeS cluster assembly protein SufB

Gene

sufB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The SufBCD complex acts synergistically with SufE to stimulate the cysteine desulfurase activity of SufS. The SufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake from extracellular iron chelators under iron limitation.1 Publication

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc

GO - Biological processi

  • iron-sulfur cluster assembly Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G6909-MONOMER.
ECOL316407:JW5273-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
FeS cluster assembly protein SufB
Gene namesi
Name:sufB
Synonyms:ynhE
Ordered Locus Names:b1683, JW5273
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13965. sufB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001473731 – 495FeS cluster assembly protein SufBAdd BLAST495

Proteomic databases

EPDiP77522.
PaxDbiP77522.
PRIDEiP77522.

Interactioni

Subunit structurei

Part of the SufBCD complex that contains SufB, SufC and SufD.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
sufAP776675EBI-562758,EBI-1125011
sufCP7749913EBI-562758,EBI-561601
sufDP7768913EBI-562758,EBI-562751

Protein-protein interaction databases

BioGridi4260282. 42 interactors.
DIPiDIP-10938N.
IntActiP77522. 5 interactors.
STRINGi511145.b1683.

Structurei

Secondary structure

1495
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 46Combined sources7
Helixi54 – 69Combined sources16
Beta strandi165 – 168Combined sources4
Helixi169 – 175Combined sources7
Helixi177 – 183Combined sources7
Beta strandi186 – 188Combined sources3
Helixi194 – 200Combined sources7
Beta strandi206 – 211Combined sources6
Beta strandi234 – 242Combined sources9
Beta strandi247 – 257Combined sources11
Beta strandi264 – 273Combined sources10
Beta strandi278 – 288Combined sources11
Turni290 – 292Combined sources3
Beta strandi298 – 307Combined sources10
Beta strandi313 – 321Combined sources9
Beta strandi324 – 328Combined sources5
Beta strandi331 – 334Combined sources4
Beta strandi340 – 349Combined sources10
Beta strandi354 – 357Combined sources4
Beta strandi360 – 363Combined sources4
Beta strandi369 – 379Combined sources11
Beta strandi383 – 392Combined sources10
Beta strandi400 – 413Combined sources14
Beta strandi415 – 424Combined sources10
Beta strandi430 – 439Combined sources10
Helixi442 – 450Combined sources9
Helixi455 – 466Combined sources12
Helixi468 – 471Combined sources4
Helixi476 – 493Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AWFX-ray2.96A/E1-495[»]
5AWGX-ray4.28A/E1-495[»]
ProteinModelPortaliP77522.
SMRiP77522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UPF0051 (ycf24) family.Curated

Phylogenomic databases

eggNOGiENOG4105DIW. Bacteria.
COG0719. LUCA.
HOGENOMiHOG000069112.
InParanoidiP77522.
KOiK09014.
OMAiYPYVDVR.
PhylomeDBiP77522.

Family and domain databases

InterProiIPR010231. SUF_FeS_clus_asmbl_SufB.
IPR000825. SUF_FeS_clus_asmbl_SufBD.
[Graphical view]
PfamiPF01458. UPF0051. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01980. sufB. 1 hit.

Sequencei

Sequence statusi: Complete.

P77522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRNTEATDD VKTWTGGPLN YKEGFFTQLA TDELAKGINE EVVRAISAKR
60 70 80 90 100
NEPEWMLEFR LNAYRAWLEM EEPHWLKAHY DKLNYQDYSY YSAPSCGNCD
110 120 130 140 150
DTCASEPGAV QQTGANAFLS KEVEAAFEQL GVPVREGKEV AVDAIFDSVS
160 170 180 190 200
VATTYREKLA EQGIIFCSFG EAIHDHPELV RKYLGTVVPG NDNFFAALNA
210 220 230 240 250
AVASDGTFIY VPKGVRCPME LSTYFRINAE KTGQFERTIL VADEDSYVSY
260 270 280 290 300
IEGCSAPVRD SYQLHAAVVE VIIHKNAEVK YSTVQNWFPG DNNTGGILNF
310 320 330 340 350
VTKRALCEGE NSKMSWTQSE TGSAITWKYP SCILRGDNSI GEFYSVALTS
360 370 380 390 400
GHQQADTGTK MIHIGKNTKS TIISKGISAG HSQNSYRGLV KIMPTATNAR
410 420 430 440 450
NFTQCDSMLI GANCGAHTFP YVECRNNSAQ LEHEATTSRI GEDQLFYCLQ
460 470 480 490
RGISEEDAIS MIVNGFCKDV FSELPLEFAV EAQKLLAISL EHSVG
Length:495
Mass (Da):54,745
Last modified:May 30, 2000 - v2
Checksum:i3BB49EEADB6C1278
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74753.2.
AP009048 Genomic DNA. Translation: BAA15454.2.
PIRiC64926.
RefSeqiNP_416198.2. NC_000913.3.
WP_000089364.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74753; AAC74753; b1683.
BAA15454; BAA15454; BAA15454.
GeneIDi945753.
KEGGiecj:JW5273.
eco:b1683.
PATRICi32118672. VBIEscCol129921_1754.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74753.2.
AP009048 Genomic DNA. Translation: BAA15454.2.
PIRiC64926.
RefSeqiNP_416198.2. NC_000913.3.
WP_000089364.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AWFX-ray2.96A/E1-495[»]
5AWGX-ray4.28A/E1-495[»]
ProteinModelPortaliP77522.
SMRiP77522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260282. 42 interactors.
DIPiDIP-10938N.
IntActiP77522. 5 interactors.
STRINGi511145.b1683.

Proteomic databases

EPDiP77522.
PaxDbiP77522.
PRIDEiP77522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74753; AAC74753; b1683.
BAA15454; BAA15454; BAA15454.
GeneIDi945753.
KEGGiecj:JW5273.
eco:b1683.
PATRICi32118672. VBIEscCol129921_1754.

Organism-specific databases

EchoBASEiEB3723.
EcoGeneiEG13965. sufB.

Phylogenomic databases

eggNOGiENOG4105DIW. Bacteria.
COG0719. LUCA.
HOGENOMiHOG000069112.
InParanoidiP77522.
KOiK09014.
OMAiYPYVDVR.
PhylomeDBiP77522.

Enzyme and pathway databases

BioCyciEcoCyc:G6909-MONOMER.
ECOL316407:JW5273-MONOMER.

Miscellaneous databases

PROiP77522.

Family and domain databases

InterProiIPR010231. SUF_FeS_clus_asmbl_SufB.
IPR000825. SUF_FeS_clus_asmbl_SufBD.
[Graphical view]
PfamiPF01458. UPF0051. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01980. sufB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSUFB_ECOLI
AccessioniPrimary (citable) accession number: P77522
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.