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Protein

Sensor histidine kinase DpiB

Gene

dpiB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox conditions. Functions as a sensor kinase that phosphorylates DpiA in the presence of citrate.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulationi

Autophosphorylation is induced in vitro by dithiothreitol (DTT).1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • cellular response to organic substance Source: EcoCyc
  • peptidyl-histidine phosphorylation Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
  • regulation of transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6345-MONOMER.
ECOL316407:JW0611-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase DpiB (EC:2.7.13.3)
Alternative name(s):
Sensor histidine kinase CitA
Gene namesi
Name:dpiB
Synonyms:citA, mpdB, ybeP
Ordered Locus Names:b0619, JW0611
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13646. dpiB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121CytoplasmicSequence analysisAdd
BLAST
Transmembranei22 – 4221HelicalSequence analysisAdd
BLAST
Topological domaini43 – 182140PeriplasmicSequence analysisAdd
BLAST
Transmembranei183 – 20321HelicalSequence analysisAdd
BLAST
Topological domaini204 – 552349CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi138 – 1381G → R: Reduces strongly citrate binding. 1 Publication
Mutagenesisi478 – 4781C → A: Loss of autophosphorylation. 1 Publication
Mutagenesisi517 – 5171C → A: Decrease in autophosphorylation. 1 Publication
Mutagenesisi529 – 5291C → A: Constitutively active. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 552552Sensor histidine kinase DpiBPRO_0000074732Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei347 – 3471Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP77510.
PRIDEiP77510.

Interactioni

Protein-protein interaction databases

BioGridi4263372. 13 interactions.
IntActiP77510. 6 interactions.
STRINGi511145.b0619.

Structurei

3D structure databases

ProteinModelPortaliP77510.
SMRiP77510. Positions 49-169, 210-322, 428-539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini222 – 29271PASAdd
BLAST
Domaini344 – 541198Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Domaini

The periplasmic domain binds citrate with high affinity. The C-terminal cytoplasmic domain senses reduced conditions using a single Cys in vitro.2 Publications

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CEQ. Bacteria.
COG3290. LUCA.
HOGENOMiHOG000241934.
KOiK07700.
OMAiRTQEGDN.
PhylomeDBiP77510.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77510-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQLNENKQF AFFQRLAFPL RIFLLILVFS IFVIAALAQY FTASFEDYLT
60 70 80 90 100
LHVRDMAMNQ AKIIASNDSV ISAVKTRDYK RLATIANKLQ RDTDFDYVVI
110 120 130 140 150
GDRHSIRLYH PNPEKIGYPM QFTKQGALEK GESYFITGKG SMGMAMRAKT
160 170 180 190 200
PIFDDDGKVI GVVSIGYLVS KIDSWRAEFL LPMAGVFVVL LGILMLLSWF
210 220 230 240 250
LAAHIRRQMM GMEPKQIARV VRQQEALFSS VYEGLIAVDP HGYITAINRN
260 270 280 290 300
ARKMLGLSSP GRQWLGKPIV EVVRPADFFT EQIDEKRQDV VANFNGLSVI
310 320 330 340 350
ANREAIRSGD DLLGAIISFR SKDEISTLNA QLTQIKQYVE SLRTLRHEHL
360 370 380 390 400
NWMSTLNGLL QMKEYDRVLA MVQGESQAQQ QLIDSLREAF ADRQVAGLLF
410 420 430 440 450
GKVQRARELG LKMIIVPGSQ LSQLPPGLDS TEFAAIVGNL LDNAFEASLR
460 470 480 490 500
SDEGNKIVEL FLSDEGDDVV IEVADQGCGV PESLRDKIFE QGVSTRADEP
510 520 530 540 550
GEHGIGLYLI ASYVTRCGGV ITLEDNDPCG TLFSIYIPKV KPNDSSINPI

DR
Length:552
Mass (Da):61,684
Last modified:February 1, 1997 - v1
Checksum:i3E3F0E081ABD6E77
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti518 – 55235GGVIT…NPIDR → VVLSLSKIMIPAVPYFQSIF RK in AAC28951 (PubMed:9701802).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46667 Genomic DNA. Translation: AAC28951.1.
U82598 Genomic DNA. Translation: AAB40819.1.
U00096 Genomic DNA. Translation: AAC73720.1.
AP009048 Genomic DNA. Translation: BAA35255.1.
PIRiA64796.
RefSeqiNP_415152.1. NC_000913.3.
WP_000939767.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73720; AAC73720; b0619.
BAA35255; BAA35255; BAA35255.
GeneIDi945233.
KEGGiecj:JW0611.
eco:b0619.
PATRICi32116418. VBIEscCol129921_0649.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46667 Genomic DNA. Translation: AAC28951.1.
U82598 Genomic DNA. Translation: AAB40819.1.
U00096 Genomic DNA. Translation: AAC73720.1.
AP009048 Genomic DNA. Translation: BAA35255.1.
PIRiA64796.
RefSeqiNP_415152.1. NC_000913.3.
WP_000939767.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77510.
SMRiP77510. Positions 49-169, 210-322, 428-539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263372. 13 interactions.
IntActiP77510. 6 interactions.
STRINGi511145.b0619.

Proteomic databases

PaxDbiP77510.
PRIDEiP77510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73720; AAC73720; b0619.
BAA35255; BAA35255; BAA35255.
GeneIDi945233.
KEGGiecj:JW0611.
eco:b0619.
PATRICi32116418. VBIEscCol129921_0649.

Organism-specific databases

EchoBASEiEB3410.
EcoGeneiEG13646. dpiB.

Phylogenomic databases

eggNOGiENOG4105CEQ. Bacteria.
COG3290. LUCA.
HOGENOMiHOG000241934.
KOiK07700.
OMAiRTQEGDN.
PhylomeDBiP77510.

Enzyme and pathway databases

BioCyciEcoCyc:G6345-MONOMER.
ECOL316407:JW0611-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP77510.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPIB_ECOLI
AccessioniPrimary (citable) accession number: P77510
Secondary accession number(s): O54338
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.