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Protein

Sensor histidine kinase DpiB

Gene

dpiB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox conditions. Functions as a sensor kinase that phosphorylates DpiA in the presence of citrate.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulationi

Autophosphorylation is induced in vitro by dithiothreitol (DTT).1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • cellular response to organic substance Source: EcoCyc
  • peptidyl-histidine phosphorylation Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
  • regulation of transcription, DNA-templated Source: InterPro

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6345-MONOMER
BRENDAi2.7.13.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase DpiB (EC:2.7.13.3)
Alternative name(s):
Sensor histidine kinase CitA
Gene namesi
Name:dpiB
Synonyms:citA, mpdB, ybeP
Ordered Locus Names:b0619, JW0611
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13646 dpiB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21
Topological domaini43 – 182PeriplasmicSequence analysisAdd BLAST140
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 552CytoplasmicSequence analysisAdd BLAST349

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi138G → R: Reduces strongly citrate binding. 1 Publication1
Mutagenesisi478C → A: Loss of autophosphorylation. 1 Publication1
Mutagenesisi517C → A: Decrease in autophosphorylation. 1 Publication1
Mutagenesisi529C → A: Constitutively active. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747321 – 552Sensor histidine kinase DpiBAdd BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei347Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP77510
PRIDEiP77510

PTM databases

iPTMnetiP77510

Interactioni

Protein-protein interaction databases

BioGridi4263372, 14 interactors
IntActiP77510, 6 interactors
STRINGi316385.ECDH10B_0687

Structurei

3D structure databases

ProteinModelPortaliP77510
SMRiP77510
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini222 – 292PASAdd BLAST71
Domaini344 – 541Histidine kinasePROSITE-ProRule annotationAdd BLAST198

Domaini

The periplasmic domain binds citrate with high affinity. The C-terminal cytoplasmic domain senses reduced conditions using a single Cys in vitro.2 Publications

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CEQ Bacteria
COG3290 LUCA
HOGENOMiHOG000241934
KOiK07700
OMAiNEKNGGA
PhylomeDBiP77510

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00130 PAS, 1 hit
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR033463 sCache_3
IPR029151 Sensor-like_sf
IPR004358 Sig_transdc_His_kin-like_C
IPR016120 Sig_transdc_His_kin_SpoOB
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00989 PAS, 1 hit
PF17203 sCache_3_2, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00091 PAS, 1 hit
SUPFAMiSSF103190 SSF103190, 1 hit
SSF55785 SSF55785, 1 hit
SSF55874 SSF55874, 1 hit
SSF55890 SSF55890, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

P77510-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQLNENKQF AFFQRLAFPL RIFLLILVFS IFVIAALAQY FTASFEDYLT
60 70 80 90 100
LHVRDMAMNQ AKIIASNDSV ISAVKTRDYK RLATIANKLQ RDTDFDYVVI
110 120 130 140 150
GDRHSIRLYH PNPEKIGYPM QFTKQGALEK GESYFITGKG SMGMAMRAKT
160 170 180 190 200
PIFDDDGKVI GVVSIGYLVS KIDSWRAEFL LPMAGVFVVL LGILMLLSWF
210 220 230 240 250
LAAHIRRQMM GMEPKQIARV VRQQEALFSS VYEGLIAVDP HGYITAINRN
260 270 280 290 300
ARKMLGLSSP GRQWLGKPIV EVVRPADFFT EQIDEKRQDV VANFNGLSVI
310 320 330 340 350
ANREAIRSGD DLLGAIISFR SKDEISTLNA QLTQIKQYVE SLRTLRHEHL
360 370 380 390 400
NWMSTLNGLL QMKEYDRVLA MVQGESQAQQ QLIDSLREAF ADRQVAGLLF
410 420 430 440 450
GKVQRARELG LKMIIVPGSQ LSQLPPGLDS TEFAAIVGNL LDNAFEASLR
460 470 480 490 500
SDEGNKIVEL FLSDEGDDVV IEVADQGCGV PESLRDKIFE QGVSTRADEP
510 520 530 540 550
GEHGIGLYLI ASYVTRCGGV ITLEDNDPCG TLFSIYIPKV KPNDSSINPI

DR
Length:552
Mass (Da):61,684
Last modified:February 1, 1997 - v1
Checksum:i3E3F0E081ABD6E77
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti518 – 552GGVIT…NPIDR → VVLSLSKIMIPAVPYFQSIF RK in AAC28951 (PubMed:9701802).CuratedAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46667 Genomic DNA Translation: AAC28951.1
U82598 Genomic DNA Translation: AAB40819.1
U00096 Genomic DNA Translation: AAC73720.1
AP009048 Genomic DNA Translation: BAA35255.1
PIRiA64796
RefSeqiNP_415152.1, NC_000913.3
WP_000939767.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73720; AAC73720; b0619
BAA35255; BAA35255; BAA35255
GeneIDi945233
KEGGiecj:JW0611
eco:b0619
PATRICifig|511145.12.peg.649

Similar proteinsi

Entry informationi

Entry nameiDPIB_ECOLI
AccessioniPrimary (citable) accession number: P77510
Secondary accession number(s): O54338
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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