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Protein

Probable ATP-dependent transporter SufC

Gene

sufC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has low ATPase activity. The SufBCD complex acts synergistically with SufE to stimulate the cysteine desulfurase activity of SufS. The SufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake from extracellular iron chelators under iron limitation.2 Publications

Kineticsi

  1. KM=0.29 mM for ATP1 Publication
  1. Vmax=4.45 µmol/min/mg enzyme1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 41ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • iron-sulfur cluster assembly Source: EcoCyc
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6908-MONOMER.
ECOL316407:JW1672-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent transporter SufC
Gene namesi
Name:sufC
Synonyms:ynhD
Ordered Locus Names:b1682, JW1672
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13964. sufC.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000929801 – 248Probable ATP-dependent transporter SufCAdd BLAST248

Proteomic databases

EPDiP77499.
PaxDbiP77499.
PRIDEiP77499.

Interactioni

Subunit structurei

Part of the SufBCD complex that contains SufB, SufC and SufD. Interacts directly with SufB and SufD.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
sufAP776675EBI-561601,EBI-1125011
sufBP7752213EBI-561601,EBI-562758
sufDP776898EBI-561601,EBI-562751

Protein-protein interaction databases

BioGridi4260279. 53 interactors.
DIPiDIP-10939N.
IntActiP77499. 16 interactors.
MINTiMINT-7290829.
STRINGi511145.b1682.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 11Combined sources10
Beta strandi14 – 24Combined sources11
Beta strandi29 – 33Combined sources5
Helixi40 – 48Combined sources9
Beta strandi54 – 62Combined sources9
Helixi67 – 69Combined sources3
Helixi72 – 78Combined sources7
Beta strandi80 – 83Combined sources4
Beta strandi88 – 90Combined sources3
Helixi95 – 108Combined sources14
Helixi109 – 111Combined sources3
Helixi117 – 130Combined sources14
Turni135 – 139Combined sources5
Turni142 – 145Combined sources4
Helixi148 – 162Combined sources15
Beta strandi165 – 171Combined sources7
Turni172 – 175Combined sources4
Helixi178 – 189Combined sources12
Beta strandi197 – 201Combined sources5
Helixi205 – 209Combined sources5
Beta strandi214 – 220Combined sources7
Beta strandi223 – 228Combined sources6
Helixi232 – 237Combined sources6
Turni238 – 240Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D3WX-ray2.50A/B/C/D1-248[»]
2ZU0X-ray2.20C/D1-248[»]
5AWFX-ray2.96C/D/G/H1-248[»]
5AWGX-ray4.28C/D/G/H1-248[»]
ProteinModelPortaliP77499.
SMRiP77499.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77499.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 246ABC transporterPROSITE-ProRule annotationAdd BLAST245

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C3K. Bacteria.
COG0396. LUCA.
InParanoidiP77499.
KOiK09013.
OMAiMAMLEPK.
PhylomeDBiP77499.

Family and domain databases

CDDicd03217. ABC_FeS_Assembly. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR010230. FeS-cluster_ATPase_SufC.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01978. sufC. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77499-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSIKDLHVS VEDKAILRGL SLDVHPGEVH AIMGPNGSGK STLSATLAGR
60 70 80 90 100
EDYEVTGGTV EFKGKDLLAL SPEDRAGEGI FMAFQYPVEI PGVSNQFFLQ
110 120 130 140 150
TALNAVRSYR GQETLDRFDF QDLMEEKIAL LKMPEDLLTR SVNVGFSGGE
160 170 180 190 200
KKRNDILQMA VLEPELCILD ESDSGLDIDA LKVVADGVNS LRDGKRSFII
210 220 230 240
VTHYQRILDY IKPDYVHVLY QGRIVKSGDF TLVKQLEEQG YGWLTEQQ
Length:248
Mass (Da):27,582
Last modified:February 1, 1997 - v1
Checksum:i78FB1108E1850DF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74752.1.
AP009048 Genomic DNA. Translation: BAA15455.1.
PIRiB64926.
RefSeqiNP_416197.1. NC_000913.3.
WP_000948863.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74752; AAC74752; b1682.
BAA15455; BAA15455; BAA15455.
GeneIDi946128.
KEGGiecj:JW1672.
eco:b1682.
PATRICi32118670. VBIEscCol129921_1753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74752.1.
AP009048 Genomic DNA. Translation: BAA15455.1.
PIRiB64926.
RefSeqiNP_416197.1. NC_000913.3.
WP_000948863.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D3WX-ray2.50A/B/C/D1-248[»]
2ZU0X-ray2.20C/D1-248[»]
5AWFX-ray2.96C/D/G/H1-248[»]
5AWGX-ray4.28C/D/G/H1-248[»]
ProteinModelPortaliP77499.
SMRiP77499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260279. 53 interactors.
DIPiDIP-10939N.
IntActiP77499. 16 interactors.
MINTiMINT-7290829.
STRINGi511145.b1682.

Proteomic databases

EPDiP77499.
PaxDbiP77499.
PRIDEiP77499.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74752; AAC74752; b1682.
BAA15455; BAA15455; BAA15455.
GeneIDi946128.
KEGGiecj:JW1672.
eco:b1682.
PATRICi32118670. VBIEscCol129921_1753.

Organism-specific databases

EchoBASEiEB3722.
EcoGeneiEG13964. sufC.

Phylogenomic databases

eggNOGiENOG4105C3K. Bacteria.
COG0396. LUCA.
InParanoidiP77499.
KOiK09013.
OMAiMAMLEPK.
PhylomeDBiP77499.

Enzyme and pathway databases

BioCyciEcoCyc:G6908-MONOMER.
ECOL316407:JW1672-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP77499.
PROiP77499.

Family and domain databases

CDDicd03217. ABC_FeS_Assembly. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR010230. FeS-cluster_ATPase_SufC.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01978. sufC. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSUFC_ECOLI
AccessioniPrimary (citable) accession number: P77499
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.