P77495 (PRPE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Propionate--CoA ligase
EC=6.2.1.17

Alternative name(s):
Propionyl-CoA synthetase

Gene names

Name:	prpE
Synonyms:	yahU
Ordered Locus Names:	b0335, JW0326

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity By similarity.
Catalytic activity	ATP + propanoate + CoA = AMP + diphosphate + propanoyl-CoA.
Pathway	Organic acid metabolism; propanoate degradation.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	propionate catabolic process, 2-methylcitrate cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW propionate-CoA ligase activity Inferred from direct assay PubMed 22834299. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	Propionate--CoA ligase		PRO_0000193188

Sequences

	Sequence		Length	Mass (Da)
	P77495 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B192E2E49ED900D8	FASTA	628	69,351
	10 20 30 40 50 60 MSFSEFYQRS INEPEQFWAE QARRIDWQTP FTQTLDHSNP PFARWFCEGR TNLCHNAIDR 70 80 90 100 110 120 WLEKQPEALA LIAVSSETEE ERTFTFRQLH DEVNAVASML RSLGVQRGDR VLVYMPMIAE 130 140 150 160 170 180 AHITLLACAR IGAIHSVVFG GFASHSVAAR IDDAKPVLIV SADAGARGGK IIPYKKLLDD 190 200 210 220 230 240 AISQAQHQPR HVLLVDRGLA KMARVSGRDV DFASLRHQHI GARVPVAWLE SNETSCILYT 250 260 270 280 290 300 SGTTGKPKGV QRDVGGYAVA LATSMDTIFG GKAGSVFFCA SDIGWVVGHS YIVYAPLLAG 310 320 330 340 350 360 MATIVYEGLP TWPDCGVWWT IVEKYQVSRM FSAPTAIRVL KKFPTAEIRK HDLSSLEVLY 370 380 390 400 410 420 LAGEPLDEPT ASWVSNTLDV PVIDNYWQTE SGWPIMAIAR GLDDRPTRLG SPGVPMYGYN 430 440 450 460 470 480 VQLLNEVTGE PCGVNEKGML VVEGPLPPGC IQTIWGDDGR FVKTYWSLFS RPVYATFDWG 490 500 510 520 530 540 IRDADGYHFI LGRTDDVINV AGHRLGTREI EESISSHPGV AEVAVVGVKD ALKGQVAVAF 550 560 570 580 590 600 VIPKESDSLE DRDVAHSQEK AIMALVDSQI GNFGRPAHVW FVSQLPKTRS GKMLRRTIQA 610 620 ICEGRDPGDL TTIDDPASLD QIRQAMEE

References

[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73857 Genomic DNA. Translation: AAB18059.1. U00096 Genomic DNA. Translation: AAC73438.1. AP009048 Genomic DNA. Translation: BAE76117.1.
PIR	G64760.
RefSeq	NP_414869.1. NC_000913.2. YP_488629.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P77495.
SMR	P77495. Positions 7-622.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77495. 7 interactions.
STRING	511145.b0335.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73438; AAC73438; b0335. BAE76117; BAE76117; BAE76117.
GeneID	12930817. 946891.
KEGG	ecj:Y75_p0324. eco:b0335.
PATRIC	32115801. VBIEscCol129921_0342.
Organism-specific databases
EchoBASE	EB3372.
EcoGene	EG13604. prpE.
Phylogenomic databases
eggNOG	COG0365.
HOGENOM	HOG000229981.
KO	K01908.
OMA	MEYLFDG.
ProtClustDB	PRK10524.
Enzyme and pathway databases
BioCyc	EcoCyc:G6200-MONOMER. ECOL316407:JW0326-MONOMER. MetaCyc:G6200-MONOMER.
UniPathway	UPA00946.
Gene expression databases
Genevestigator	P77495.
Family and domain databases
InterPro	IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR025110. DUF4009. IPR012694. Propion_PrpE. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. PF13193. DUF4009. 1 hit. [Graphical view]
TIGRFAMs	TIGR02316. propion_prpE. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_ECOLI

Accession

Primary (citable) accession number: P77495
Secondary accession number(s): Q2MC89

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program