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Protein

Propionate--CoA ligase

Gene

prpE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity (By similarity).By similarity

Catalytic activityi

ATP + propanoate + CoA = AMP + diphosphate + propanoyl-CoA.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. propionate-CoA ligase activity Source: EcoCyc

GO - Biological processi

  1. propionate catabolic process, 2-methylcitrate cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6200-MONOMER.
ECOL316407:JW0326-MONOMER.
MetaCyc:G6200-MONOMER.
UniPathwayiUPA00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Propionate--CoA ligase (EC:6.2.1.17)
Alternative name(s):
Propionyl-CoA synthetase
Gene namesi
Name:prpE
Synonyms:yahU
Ordered Locus Names:b0335, JW0326
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13604. prpE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 628628Propionate--CoA ligasePRO_0000193188Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP77495.

Interactioni

Protein-protein interaction databases

IntActiP77495. 7 interactions.
STRINGi511145.b0335.

Structurei

3D structure databases

ProteinModelPortaliP77495.
SMRiP77495. Positions 7-622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
InParanoidiP77495.
KOiK01908.
OMAiWGSDERF.
OrthoDBiEOG68WR2H.
PhylomeDBiP77495.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR012694. Propion_PrpE.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02316. propion_prpE. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77495-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSEFYQRS INEPEQFWAE QARRIDWQTP FTQTLDHSNP PFARWFCEGR
60 70 80 90 100
TNLCHNAIDR WLEKQPEALA LIAVSSETEE ERTFTFRQLH DEVNAVASML
110 120 130 140 150
RSLGVQRGDR VLVYMPMIAE AHITLLACAR IGAIHSVVFG GFASHSVAAR
160 170 180 190 200
IDDAKPVLIV SADAGARGGK IIPYKKLLDD AISQAQHQPR HVLLVDRGLA
210 220 230 240 250
KMARVSGRDV DFASLRHQHI GARVPVAWLE SNETSCILYT SGTTGKPKGV
260 270 280 290 300
QRDVGGYAVA LATSMDTIFG GKAGSVFFCA SDIGWVVGHS YIVYAPLLAG
310 320 330 340 350
MATIVYEGLP TWPDCGVWWT IVEKYQVSRM FSAPTAIRVL KKFPTAEIRK
360 370 380 390 400
HDLSSLEVLY LAGEPLDEPT ASWVSNTLDV PVIDNYWQTE SGWPIMAIAR
410 420 430 440 450
GLDDRPTRLG SPGVPMYGYN VQLLNEVTGE PCGVNEKGML VVEGPLPPGC
460 470 480 490 500
IQTIWGDDGR FVKTYWSLFS RPVYATFDWG IRDADGYHFI LGRTDDVINV
510 520 530 540 550
AGHRLGTREI EESISSHPGV AEVAVVGVKD ALKGQVAVAF VIPKESDSLE
560 570 580 590 600
DRDVAHSQEK AIMALVDSQI GNFGRPAHVW FVSQLPKTRS GKMLRRTIQA
610 620
ICEGRDPGDL TTIDDPASLD QIRQAMEE
Length:628
Mass (Da):69,351
Last modified:February 1, 1997 - v1
Checksum:iB192E2E49ED900D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18059.1.
U00096 Genomic DNA. Translation: AAC73438.1.
AP009048 Genomic DNA. Translation: BAE76117.1.
PIRiG64760.
RefSeqiNP_414869.1. NC_000913.3.
YP_488629.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73438; AAC73438; b0335.
BAE76117; BAE76117; BAE76117.
GeneIDi12930817.
946891.
KEGGiecj:Y75_p0324.
eco:b0335.
PATRICi32115801. VBIEscCol129921_0342.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18059.1.
U00096 Genomic DNA. Translation: AAC73438.1.
AP009048 Genomic DNA. Translation: BAE76117.1.
PIRiG64760.
RefSeqiNP_414869.1. NC_000913.3.
YP_488629.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP77495.
SMRiP77495. Positions 7-622.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP77495. 7 interactions.
STRINGi511145.b0335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73438; AAC73438; b0335.
BAE76117; BAE76117; BAE76117.
GeneIDi12930817.
946891.
KEGGiecj:Y75_p0324.
eco:b0335.
PATRICi32115801. VBIEscCol129921_0342.

Organism-specific databases

EchoBASEiEB3372.
EcoGeneiEG13604. prpE.

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
InParanoidiP77495.
KOiK01908.
OMAiWGSDERF.
OrthoDBiEOG68WR2H.
PhylomeDBiP77495.

Enzyme and pathway databases

UniPathwayiUPA00946.
BioCyciEcoCyc:G6200-MONOMER.
ECOL316407:JW0326-MONOMER.
MetaCyc:G6200-MONOMER.

Miscellaneous databases

PROiP77495.

Gene expression databases

GenevestigatoriP77495.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR012694. Propion_PrpE.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02316. propion_prpE. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPRPE_ECOLI
AccessioniPrimary (citable) accession number: P77495
Secondary accession number(s): Q2MC89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.