P77489 (YAGR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative xanthine dehydrogenase YagR molybdenum-binding subunit
EC=1.17.1.4

Gene names

Name:	yagR
Ordered Locus Names:	b0284, JW0278

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH.
Cofactor	Binds 1 molybdenum ion (molybdopterin) per subunit By similarity.
Subunit structure	Heterotrimer of YagR, YagS and YagT Potential.
Sequence similarities	Belongs to the xanthine dehydrogenase family.

Ontologies

Keywords
Biological process	Purine metabolism Purine salvage
Ligand	Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Cellular_component	periplasmic space Non-traceable author statement PubMed 19368556. Source: EcoCyc
Molecular_function	molybdenum ion binding Inferred from direct assay PubMed 19368556. Source: EcoCyc oxidoreductase activity, acting on the aldehyde or oxo group of donors Inferred from direct assay PubMed 19368556. Source: EcoCyc xanthine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	Putative xanthine dehydrogenase YagR molybdenum-binding subunit		PRO_0000166090
Sites
	Metal binding	211	1	Molybdenum Potential
	Metal binding	242	1	Molybdenum; via carbonyl oxygen Potential
	Metal binding	350	1	Molybdenum; via amide nitrogen Potential

Sequences

	Sequence		Length	Mass (Da)
	P77489 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: AF198715794F0138	FASTA	732	78,088
	10 20 30 40 50 60 MKFDKPAGEN PIDQLKVVGR PHDRIDGPLK TTGTARYAYE WHEEAPNAAY GYIVGSAIAK 70 80 90 100 110 120 GRLTALDTDA AQKAPGVLAV ITASNAGALG KGDKNTARLL GGPTIEHYHQ AIALVVAETF 130 140 150 160 170 180 EQARAAASLV QAHYRRNKGA YSLADEKQAV NQPPEDTPDK NVGDFDGAFT SAAVKIDATY 190 200 210 220 230 240 TTPDQSHMAM EPHASMAVWD GNKLTLWTSN QMIDWCRTDL AKTLKVPVEN VRIISPYIGG 250 260 270 280 290 300 GFGGKLFLRS DALLAALAAR AVKRPVKVML PRPSIPNNTT HRPATLQHLR IGADQSGKIT 310 320 330 340 350 360 AISHESWSGN LPGGTPETAV QQSELLYAGA NRHTGLRLAT LDLPEGNAMR APGEAPGLMA 370 380 390 400 410 420 LEIAIDELAE KAGIDPVEFR ILNDTQVDPA DPTRCFSRRQ LIECLRTGAD KFGWKQRNAT 430 440 450 460 470 480 PGQVRDGEWL VGHGVAAGFR NNLLEKSGAR VHLEQNGTVT VETDMTDIGT GSYTILAQTA 490 500 510 520 530 540 AEMLGVPLEQ VAVHLGDSSF PVSAGSGGQW GANTSTSGVY AACMKLREMI ASAVGFDPEQ 550 560 570 580 590 600 SQFADGKITN GTRSATLHEA TAGGRLTAEE SIEFGTLSKE YQQSTFAGHF VEVGVHSATG 610 620 630 640 650 660 EVRVRRMLAV CAAGRILNPK TARSQVIGAM TMGMGAALME ELAVDDRLGY FVNHDMAGYE 670 680 690 700 710 720 VPVHADIPKQ EVIFLDDTDP ISSPMKAKGV GELGLCGVSA AIANAVYNAT GIRVRDYPIT 730 LDKLLDKLPD VV

References

[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73857 Genomic DNA. Translation: AAB18013.1. U00096 Genomic DNA. Translation: AAC73387.1. AP009048 Genomic DNA. Translation: BAE76068.1.
PIR	D64754.
RefSeq	NP_414818.1. NC_000913.2. YP_488580.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P77489.
SMR	P77489. Positions 16-728.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77489. 10 interactions.
STRING	511145.b0284.
Proteomic databases
PaxDb	P77489.
PRIDE	P77489.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73387; AAC73387; b0284. BAE76068; BAE76068; BAE76068.
GeneID	12932498. 944961.
KEGG	ecj:Y75_p0276. eco:b0284.
PATRIC	32115691. VBIEscCol129921_0288.
Organism-specific databases
EchoBASE	EB3327.
EcoGene	EG13557. yagR.
Phylogenomic databases
eggNOG	COG1529.
HOGENOM	HOG000244716.
KO	K11177.
OMA	DESHAMM.
ProtClustDB	CLSK879611.
Enzyme and pathway databases
BioCyc	EcoCyc:G6155-MONOMER. ECOL316407:JW0278-MONOMER. MetaCyc:G6155-MONOMER.
Gene expression databases
Genevestigator	P77489.
Family and domain databases
Gene3D	3.30.365.10. 6 hits. 3.90.1170.50. 1 hit.
InterPro	IPR000674. Ald_Oxase/Xan_DH_a/b. IPR008274. AldOxase/xan_DH_Mopterin-bd. [Graphical view]
Pfam	PF01315. Ald_Xan_dh_C. 1 hit. PF02738. Ald_Xan_dh_C2. 1 hit. [Graphical view]
SMART	SM01008. Ald_Xan_dh_C. 1 hit. [Graphical view]
SUPFAM	SSF56003. Ald_xan_DH_mo_bd. 1 hit. SSF54665. Aldxan_dh_hamm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

YAGR_ECOLI

Accession

Primary (citable) accession number: P77489
Secondary accession number(s): Q2MCD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program