Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sensor kinase CusS

Gene

cusS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Activates CusR by phosphorylation.3 Publications

Miscellaneous

The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoliWiki

GO - Biological processi

  • cellular response to copper ion Source: EcoCyc
  • cellular response to silver ion Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • phosphorylation Source: EcoliWiki
  • positive regulation of gene expression Source: EcoCyc
  • response to copper ion Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Copper, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6318-MONOMER
BRENDAi2.7.13.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor kinase CusS (EC:2.7.13.3)
Gene namesi
Name:cusS
Synonyms:ybcZ
Ordered Locus Names:b0570, JW5082
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13642 cusS

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 186PeriplasmicSequence analysisAdd BLAST150
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Topological domaini208 – 480CytoplasmicSequence analysisAdd BLAST273

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747241 – 480Sensor kinase CusSAdd BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei271Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP77485
PRIDEiP77485

Interactioni

Protein-protein interaction databases

BioGridi4259499, 18 interactors
DIPiDIP-9349N
IntActiP77485, 2 interactors
STRINGi316385.ECDH10B_0636

Structurei

Secondary structure

1480
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi39 – 62Combined sources24
Helixi69 – 81Combined sources13
Beta strandi84 – 91Combined sources8
Beta strandi97 – 100Combined sources4
Helixi107 – 113Combined sources7
Beta strandi126 – 128Combined sources3
Helixi139 – 141Combined sources3
Beta strandi148 – 159Combined sources12
Beta strandi162 – 172Combined sources11
Helixi174 – 183Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5KU5X-ray2.15A/B/C/D39-187[»]
ProteinModelPortaliP77485
SMRiP77485
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 260HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini268 – 480Histidine kinasePROSITE-ProRule annotationAdd BLAST213

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GR3 Bacteria
COG0642 LUCA
HOGENOMiHOG000126763
InParanoidiP77485
KOiK07644
OMAiQIATIHR
PhylomeDBiP77485

Family and domain databases

CDDicd06225 HAMP, 1 hit
cd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR006290 CztS_silS_copS
IPR003660 HAMP_dom
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR004358 Sig_transdc_His_kin-like_C
PfamiView protein in Pfam
PF00672 HAMP, 1 hit
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00304 HAMP, 1 hit
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SUPFAMiSSF47384 SSF47384, 1 hit
SSF55874 SSF55874, 1 hit
TIGRFAMsiTIGR01386 cztS_silS_copS, 1 hit
PROSITEiView protein in PROSITE
PS50885 HAMP, 1 hit
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

P77485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSKPFQRPF SLATRLTFFI SLATIAAFFA FAWIMIHSVK VHFAEQDIND
60 70 80 90 100
LKEISATLER VLNHPDETQA RRLMTLEDIV SGYSNVLISL ADSQGKTVYH
110 120 130 140 150
SPGAPDIREF TRDAIPDKDA QGGEVYLLSG PTMMMPGHGH GHMEHSNWRM
160 170 180 190 200
INLPVGPLVD GKPIYTLYIA LSIDFHLHYI NDLMNKLIMT ASVISILIVF
210 220 230 240 250
IVLLAVHKGH APIRSVSRQI QNITSKDLDV RLDPQTVPIE LEQLVLSFNH
260 270 280 290 300
MIERIEDVFT RQSNFSADIA HEIRTPITNL ITQTEIALSQ SRSQKELEDV
310 320 330 340 350
LYSNLEELTR MAKMVSDMLF LAQADNNQLI PEKKMLNLAD EVGKVFDFFE
360 370 380 390 400
ALAEDRGVEL RFVGDKCQVA GDPLMLRRAL SNLLSNALRY TPTGETIVVR
410 420 430 440 450
CQTVDHLVQV IVENPGTPIA PEHLPRLFDR FYRVDPSRQR KGEGSGIGLA
460 470 480
IVKSIVVAHK GTVAVTSDAR GTRFVITLPA
Length:480
Mass (Da):53,738
Last modified:February 1, 1997 - v1
Checksum:iB3D78C0810241519
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245661 Genomic DNA Translation: AAF70176.1
U82598 Genomic DNA Translation: AAB40768.1
U00096 Genomic DNA Translation: AAC73671.1
AP009048 Genomic DNA Translation: BAA35204.2
PIRiH64789
RefSeqiNP_415102.1, NC_000913.3
WP_000253839.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73671; AAC73671; b0570
BAA35204; BAA35204; BAA35204
GeneIDi945978
KEGGiecj:JW5082
eco:b0570
PATRICifig|1411691.4.peg.1704

Similar proteinsi

Entry informationi

Entry nameiCUSS_ECOLI
AccessioniPrimary (citable) accession number: P77485
Secondary accession number(s): Q9R7T9, Q9R7U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health