Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sensor kinase CusS

Gene

cusS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Activates CusR by phosphorylation.3 Publications

Miscellaneous

The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoliWiki

GO - Biological processi

  • cellular response to copper ion Source: EcoCyc
  • cellular response to silver ion Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • phosphorylation Source: EcoliWiki
  • positive regulation of gene expression Source: EcoCyc
  • response to copper ion Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Copper, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6318-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor kinase CusS (EC:2.7.13.3)
Gene namesi
Name:cusS
Synonyms:ybcZ
Ordered Locus Names:b0570, JW5082
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13642. cusS.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 186PeriplasmicSequence analysisAdd BLAST150
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Topological domaini208 – 480CytoplasmicSequence analysisAdd BLAST273

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747241 – 480Sensor kinase CusSAdd BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei271Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP77485.
PRIDEiP77485.

Interactioni

Protein-protein interaction databases

BioGridi4259499. 1 interactor.
DIPiDIP-9349N.
IntActiP77485. 2 interactors.
STRINGi316385.ECDH10B_0636.

Structurei

Secondary structure

1480
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi39 – 62Combined sources24
Helixi69 – 81Combined sources13
Beta strandi84 – 91Combined sources8
Beta strandi97 – 100Combined sources4
Helixi107 – 113Combined sources7
Beta strandi126 – 128Combined sources3
Helixi139 – 141Combined sources3
Beta strandi148 – 159Combined sources12
Beta strandi162 – 172Combined sources11
Helixi174 – 183Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5KU5X-ray2.15A/B/C/D39-187[»]
ProteinModelPortaliP77485.
SMRiP77485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 260HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini268 – 480Histidine kinasePROSITE-ProRule annotationAdd BLAST213

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GR3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000126763.
InParanoidiP77485.
KOiK07644.
PhylomeDBiP77485.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
cd00075. HATPase_c. 1 hit.
cd00082. HisKA. 1 hit.
Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR006290. CztS_silS_copS.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR036890. HATPase_C_sf.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR036097. HisK_dim/P_sf.
IPR004358. Sig_transdc_His_kin-like_C.
PfamiView protein in Pfam
PF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01386. cztS_silS_copS. 1 hit.
PROSITEiView protein in PROSITE
PS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P77485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSKPFQRPF SLATRLTFFI SLATIAAFFA FAWIMIHSVK VHFAEQDIND
60 70 80 90 100
LKEISATLER VLNHPDETQA RRLMTLEDIV SGYSNVLISL ADSQGKTVYH
110 120 130 140 150
SPGAPDIREF TRDAIPDKDA QGGEVYLLSG PTMMMPGHGH GHMEHSNWRM
160 170 180 190 200
INLPVGPLVD GKPIYTLYIA LSIDFHLHYI NDLMNKLIMT ASVISILIVF
210 220 230 240 250
IVLLAVHKGH APIRSVSRQI QNITSKDLDV RLDPQTVPIE LEQLVLSFNH
260 270 280 290 300
MIERIEDVFT RQSNFSADIA HEIRTPITNL ITQTEIALSQ SRSQKELEDV
310 320 330 340 350
LYSNLEELTR MAKMVSDMLF LAQADNNQLI PEKKMLNLAD EVGKVFDFFE
360 370 380 390 400
ALAEDRGVEL RFVGDKCQVA GDPLMLRRAL SNLLSNALRY TPTGETIVVR
410 420 430 440 450
CQTVDHLVQV IVENPGTPIA PEHLPRLFDR FYRVDPSRQR KGEGSGIGLA
460 470 480
IVKSIVVAHK GTVAVTSDAR GTRFVITLPA
Length:480
Mass (Da):53,738
Last modified:February 1, 1997 - v1
Checksum:iB3D78C0810241519
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245661 Genomic DNA. Translation: AAF70176.1.
U82598 Genomic DNA. Translation: AAB40768.1.
U00096 Genomic DNA. Translation: AAC73671.1.
AP009048 Genomic DNA. Translation: BAA35204.2.
PIRiH64789.
RefSeqiNP_415102.1. NC_000913.3.
WP_000253839.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73671; AAC73671; b0570.
BAA35204; BAA35204; BAA35204.
GeneIDi945978.
KEGGiecj:JW5082.
eco:b0570.
PATRICifig|1411691.4.peg.1704.

Similar proteinsi

Entry informationi

Entry nameiCUSS_ECOLI
AccessioniPrimary (citable) accession number: P77485
Secondary accession number(s): Q9R7T9, Q9R7U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 25, 2017
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references