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Protein

Sensor kinase CusS

Gene

cusS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Activates CusR by phosphorylation.3 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoliWiki

GO - Biological processi

  • cellular response to copper ion Source: EcoCyc
  • cellular response to silver ion Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • phosphorylation Source: EcoliWiki
  • positive regulation of gene expression Source: EcoCyc
  • response to copper ion Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Copper, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6318-MONOMER.
ECOL316407:JW5082-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor kinase CusS (EC:2.7.13.3)
Gene namesi
Name:cusS
Synonyms:ybcZ
Ordered Locus Names:b0570, JW5082
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13642. cusS.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence analysisAdd
BLAST
Transmembranei16 – 3621HelicalSequence analysisAdd
BLAST
Topological domaini37 – 186150PeriplasmicSequence analysisAdd
BLAST
Transmembranei187 – 20721HelicalSequence analysisAdd
BLAST
Topological domaini208 – 480273CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Sensor kinase CusSPRO_0000074724Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei271 – 2711Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP77485.
PRIDEiP77485.

Interactioni

Protein-protein interaction databases

BioGridi4259499. 1 interaction.
DIPiDIP-9349N.
IntActiP77485. 2 interactions.
STRINGi511145.b0570.

Structurei

3D structure databases

ProteinModelPortaliP77485.
SMRiP77485. Positions 212-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini208 – 26053HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini268 – 480213Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GR3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000126763.
InParanoidiP77485.
KOiK07644.
OMAiFVGDKRQ.
PhylomeDBiP77485.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR006290. CztS_silS_copS.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01386. cztS_silS_copS. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSKPFQRPF SLATRLTFFI SLATIAAFFA FAWIMIHSVK VHFAEQDIND
60 70 80 90 100
LKEISATLER VLNHPDETQA RRLMTLEDIV SGYSNVLISL ADSQGKTVYH
110 120 130 140 150
SPGAPDIREF TRDAIPDKDA QGGEVYLLSG PTMMMPGHGH GHMEHSNWRM
160 170 180 190 200
INLPVGPLVD GKPIYTLYIA LSIDFHLHYI NDLMNKLIMT ASVISILIVF
210 220 230 240 250
IVLLAVHKGH APIRSVSRQI QNITSKDLDV RLDPQTVPIE LEQLVLSFNH
260 270 280 290 300
MIERIEDVFT RQSNFSADIA HEIRTPITNL ITQTEIALSQ SRSQKELEDV
310 320 330 340 350
LYSNLEELTR MAKMVSDMLF LAQADNNQLI PEKKMLNLAD EVGKVFDFFE
360 370 380 390 400
ALAEDRGVEL RFVGDKCQVA GDPLMLRRAL SNLLSNALRY TPTGETIVVR
410 420 430 440 450
CQTVDHLVQV IVENPGTPIA PEHLPRLFDR FYRVDPSRQR KGEGSGIGLA
460 470 480
IVKSIVVAHK GTVAVTSDAR GTRFVITLPA
Length:480
Mass (Da):53,738
Last modified:February 1, 1997 - v1
Checksum:iB3D78C0810241519
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245661 Genomic DNA. Translation: AAF70176.1.
U82598 Genomic DNA. Translation: AAB40768.1.
U00096 Genomic DNA. Translation: AAC73671.1.
AP009048 Genomic DNA. Translation: BAA35204.2.
PIRiH64789.
RefSeqiNP_415102.1. NC_000913.3.
WP_000253839.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73671; AAC73671; b0570.
BAA35204; BAA35204; BAA35204.
GeneIDi945978.
KEGGiecj:JW5082.
eco:b0570.
PATRICi32116308. VBIEscCol129921_0594.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245661 Genomic DNA. Translation: AAF70176.1.
U82598 Genomic DNA. Translation: AAB40768.1.
U00096 Genomic DNA. Translation: AAC73671.1.
AP009048 Genomic DNA. Translation: BAA35204.2.
PIRiH64789.
RefSeqiNP_415102.1. NC_000913.3.
WP_000253839.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77485.
SMRiP77485. Positions 212-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259499. 1 interaction.
DIPiDIP-9349N.
IntActiP77485. 2 interactions.
STRINGi511145.b0570.

Proteomic databases

PaxDbiP77485.
PRIDEiP77485.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73671; AAC73671; b0570.
BAA35204; BAA35204; BAA35204.
GeneIDi945978.
KEGGiecj:JW5082.
eco:b0570.
PATRICi32116308. VBIEscCol129921_0594.

Organism-specific databases

EchoBASEiEB3406.
EcoGeneiEG13642. cusS.

Phylogenomic databases

eggNOGiENOG4105GR3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000126763.
InParanoidiP77485.
KOiK07644.
OMAiFVGDKRQ.
PhylomeDBiP77485.

Enzyme and pathway databases

BioCyciEcoCyc:G6318-MONOMER.
ECOL316407:JW5082-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP77485.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR006290. CztS_silS_copS.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01386. cztS_silS_copS. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUSS_ECOLI
AccessioniPrimary (citable) accession number: P77485
Secondary accession number(s): Q9R7T9, Q9R7U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.