Reviewed, UniProtKB/Swiss-Prot P77485 (CUSS_ECOLI)

Names and origin

Protein names

Recommended name:
    Sensor kinase cusS
    EC=2.7.13.3

Gene names

Name:	cusS
Synonyms:	ybcZ
Ordered Locus Names:	b0570, JW5082

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Member of the two-component regulatory system cusS/cusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates cusR by phosphorylation. Ref.1 Ref.6 Ref.7
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Miscellaneous	The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Copper Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro sensory perception Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Sensor kinase cusS		PRO_0000074724
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 36	21	Potential
	Topological domain	37 – 186	150	Periplasmic Potential
	Transmembrane	187 – 207	21	Potential
	Topological domain	208 – 480	273	Cytoplasmic Potential
	Domain	208 – 260	53	HAMP
	Domain	268 – 480	213	Histidine kinase
Amino acid modifications
	Modified residue	271	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77485-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B3D78C0810241519	FASTA	480	53,738
	10 20 30 40 50 60 MVSKPFQRPF SLATRLTFFI SLATIAAFFA FAWIMIHSVK VHFAEQDIND LKEISATLER 70 80 90 100 110 120 VLNHPDETQA RRLMTLEDIV SGYSNVLISL ADSQGKTVYH SPGAPDIREF TRDAIPDKDA 130 140 150 160 170 180 QGGEVYLLSG PTMMMPGHGH GHMEHSNWRM INLPVGPLVD GKPIYTLYIA LSIDFHLHYI 190 200 210 220 230 240 NDLMNKLIMT ASVISILIVF IVLLAVHKGH APIRSVSRQI QNITSKDLDV RLDPQTVPIE 250 260 270 280 290 300 LEQLVLSFNH MIERIEDVFT RQSNFSADIA HEIRTPITNL ITQTEIALSQ SRSQKELEDV 310 320 330 340 350 360 LYSNLEELTR MAKMVSDMLF LAQADNNQLI PEKKMLNLAD EVGKVFDFFE ALAEDRGVEL 370 380 390 400 410 420 RFVGDKCQVA GDPLMLRRAL SNLLSNALRY TPTGETIVVR CQTVDHLVQV IVENPGTPIA 430 440 450 460 470 480 PEHLPRLFDR FYRVDPSRQR KGEGSGIGLA IVKSIVVAHK GTVAVTSDAR GTRFVITLPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12." Munson G.P., Lam D.L., Outten F.W., O'Halloran T.V. J. Bacteriol. 182:5864-5871(2000) [PubMed: 11004187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: K12 / DH5-alpha.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli." Outten F.W., Huffman D.L., Hale J.A., O'Halloran T.V. J. Biol. Chem. 276:30670-30677(2001) [PubMed: 11399769] [Abstract] Cited for: FUNCTION IN COPPER HOMEOSTASIS. Strain: K12.
[7]	"The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions." Franke S., Grass G., Nies D.H. Microbiology 147:965-972(2001) [PubMed: 11283292] [Abstract] Cited for: PROBABLE FUNCTION IN SILVER HOMEOSTASIS. Strain: K38.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF245661 Genomic DNA. Translation: AAF70176.1. U82598 Genomic DNA. Translation: AAB40768.1. U00096 Genomic DNA. Translation: AAC73671.1. AP009048 Genomic DNA. Translation: BAA35204.2.
PIR	H64789.
RefSeq	AP_001215.1. NP_415102.1.
3D structure databases
HSSP	HSSP built from PDB template 1BXD based on UniProtKB P02933.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9349N.
STRING	P77485.
Genome annotation databases
GeneID	945978.
GenomeReviews	Gene locus JW5082 in contig AP009048_GR. Gene locus b0570 in contig U00096_GR.
KEGG	ecj:JW5082. eco:b0570.
Organism-specific databases
EchoBASE	EB3406.
EcoGene	EG13642. cusS.
CMR	Search...
Phylogenomic databases
HOGENOM	P77485.
OMA	TRETIVV.
Enzyme and pathway databases
BioCyc	EcoCyc:G6318-MON.
BRENDA	2.7.13.3. 246.
Gene expression databases
Genevestigator	P77485.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR006290. Sig_transdc_His_kin_metal-sen. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01386. cztS_silS_copS. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CUSS_ECOLI

Accession

Primary (citable) accession number: P77485
Secondary accession number(s): Q9R7T9, Q9R7U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)