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Protein

Fructose-1-phosphate phosphatase YqaB

Gene

yqaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes strongly the dephosphorylation of fructose-1-phosphate (Fru1P) and slightly the dephosphorylation of 6-phosphogluconate (6P-Glu). It has low beta-phosphoglucomutase activity.2 Publications

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Zn2+1 PublicationNote: Magnesium. Can also use other divalent metal cations as manganese, cobalt or zinc.1 Publication

Kineticsi

  1. KM=1 mM for Fru1P (with magnesium ions as cofactor and at pH 9)1 Publication
  2. KM=3.9 mM for 6P-Glu (with magnesium ions as cofactor and at pH 9)1 Publication

    pH dependencei

    Optimum pH is between 6 and 7.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei11 – 111NucleophileBy similarity
    Metal bindingi11 – 111MagnesiumBy similarity
    Metal bindingi13 – 131MagnesiumBy similarity
    Metal bindingi167 – 1671MagnesiumBy similarity

    GO - Molecular functioni

    • beta-phosphoglucomutase activity Source: EcoliWiki
    • magnesium ion binding Source: UniProtKB
    • phosphatase activity Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G7408-MONOMER.
    ECOL316407:JW2665-MONOMER.
    MetaCyc:G7408-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-1-phosphate phosphatase YqaB (EC:3.1.3.-)
    Alternative name(s):
    Fructose-1-phosphatase
    Gene namesi
    Name:yqaB
    Ordered Locus Names:b2690, JW2665
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13530. yqaB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 188188Fructose-1-phosphate phosphatase YqaBPRO_0000108063Add
    BLAST

    Proteomic databases

    EPDiP77475.
    PaxDbiP77475.

    Interactioni

    Protein-protein interaction databases

    BioGridi4262273. 22 interactions.
    DIPiDIP-12842N.
    IntActiP77475. 5 interactions.
    MINTiMINT-1265616.
    STRINGi511145.b2690.

    Structurei

    3D structure databases

    ProteinModelPortaliP77475.
    SMRiP77475. Positions 7-183.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni11 – 133Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107VXZ. Bacteria.
    COG0637. LUCA.
    HOGENOMiHOG000248341.
    InParanoidiP77475.
    OMAiCAQRMGI.
    PhylomeDBiP77475.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR010976. B-phosphoglucomutase_hydrolase.
    IPR023214. HAD-like_dom.
    IPR006439. HAD-SF_hydro_IA.
    [Graphical view]
    PfamiPF13419. HAD_2. 1 hit.
    [Graphical view]
    PRINTSiPR00413. HADHALOGNASE.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01509. HAD-SF-IA-v3. 1 hit.
    TIGR02009. PGMB-YQAB-SF. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P77475-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MYERYAGLIF DMDGTILDTE PTHRKAWREV LGHYGLQYDI QAMIALNGSP
    60 70 80 90 100
    TWRIAQAIIE LNQADLDPHA LAREKTEAVR SMLLDSVEPL PLVDVVKSWH
    110 120 130 140 150
    GRRPMAVGTG SESAIAEALL AHLGLRHYFD AVVAADHVKH HKPAPDTFLL
    160 170 180
    CAQRMGVQPT QCVVFEDADF GIQAARAAGM DAVDVRLL
    Length:188
    Mass (Da):20,780
    Last modified:February 1, 1997 - v1
    Checksum:iAA3F9FF1DF3B2024
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75737.1.
    AP009048 Genomic DNA. Translation: BAA16557.1.
    PIRiC65049.
    RefSeqiNP_417175.1. NC_000913.3.
    WP_000273290.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75737; AAC75737; b2690.
    BAA16557; BAA16557; BAA16557.
    GeneIDi945776.
    KEGGiecj:JW2665.
    eco:b2690.
    PATRICi32120774. VBIEscCol129921_2784.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75737.1.
    AP009048 Genomic DNA. Translation: BAA16557.1.
    PIRiC65049.
    RefSeqiNP_417175.1. NC_000913.3.
    WP_000273290.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP77475.
    SMRiP77475. Positions 7-183.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262273. 22 interactions.
    DIPiDIP-12842N.
    IntActiP77475. 5 interactions.
    MINTiMINT-1265616.
    STRINGi511145.b2690.

    Proteomic databases

    EPDiP77475.
    PaxDbiP77475.

    Protocols and materials databases

    DNASUi945776.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75737; AAC75737; b2690.
    BAA16557; BAA16557; BAA16557.
    GeneIDi945776.
    KEGGiecj:JW2665.
    eco:b2690.
    PATRICi32120774. VBIEscCol129921_2784.

    Organism-specific databases

    EchoBASEiEB3301.
    EcoGeneiEG13530. yqaB.

    Phylogenomic databases

    eggNOGiENOG4107VXZ. Bacteria.
    COG0637. LUCA.
    HOGENOMiHOG000248341.
    InParanoidiP77475.
    OMAiCAQRMGI.
    PhylomeDBiP77475.

    Enzyme and pathway databases

    BioCyciEcoCyc:G7408-MONOMER.
    ECOL316407:JW2665-MONOMER.
    MetaCyc:G7408-MONOMER.

    Miscellaneous databases

    PROiP77475.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR010976. B-phosphoglucomutase_hydrolase.
    IPR023214. HAD-like_dom.
    IPR006439. HAD-SF_hydro_IA.
    [Graphical view]
    PfamiPF13419. HAD_2. 1 hit.
    [Graphical view]
    PRINTSiPR00413. HADHALOGNASE.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01509. HAD-SF-IA-v3. 1 hit.
    TIGR02009. PGMB-YQAB-SF. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiYQAB_ECOLI
    AccessioniPrimary (citable) accession number: P77475
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: September 7, 2016
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.