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Protein

Outer membrane usher protein SfmD

Gene

sfmD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Part of the sfmACDHF fimbrial operon. Could contribute to adhesion to various surfaces in specific environmental niches. Increases adhesion to eukaryotic T24 bladder epithelial cells in the absence of fim genes. Probably involved in the export and assembly of fimbrial subunits across the outer membrane.1 Publication

GO - Molecular functioni

GO - Biological processi

  • pilus assembly Source: GO_Central
  • pilus organization Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Fimbrium biogenesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6292-MONOMER.
ECOL316407:JW0521-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane usher protein SfmD
Gene namesi
Name:sfmD
Ordered Locus Names:b0532, JW0521
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13883. sfmD.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the operon under classical laboratory conditions does not result in any major effect on E.coli capacity to form biofilms compared with the wild-type strain.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000000931336 – 867Outer membrane usher protein SfmDAdd BLAST832

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi840 ↔ 862Sequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP77468.
PRIDEiP77468.

Expressioni

Inductioni

Expression is negatively regulated by H-NS and subjected to cAMP receptor protein (CRP)-mediated catabolite repression.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263216. 173 interactors.
IntActiP77468. 2 interactors.
STRINGi511145.b0532.

Structurei

3D structure databases

ProteinModelPortaliP77468.
SMRiP77468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fimbrial export usher family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CW0. Bacteria.
COG3188. LUCA.
HOGENOMiHOG000111414.
InParanoidiP77468.
KOiK07354.
OMAiGSASANW.
PhylomeDBiP77468.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIPTTTDIP QRYTWCLAGI CYSSLAILPS FLSYAESYFN PAFLLENGTS
60 70 80 90 100
VADLSRFERG NHQPAGVYRV DLWRNDEFIG SQDIVFESTT ENTGDKSGGL
110 120 130 140 150
MPCFNQVLLE RIGLNSSAFP ELAQQQNNKC INLLKAVPDA TINFDFAAMR
160 170 180 190 200
LNITIPQIAL LSSAHGYIPP EEWDEGIPAL LLNYNFTGNR GNGNDSYFFS
210 220 230 240 250
ELSGINIGPW RLRNNGSWNY FRGNGYHSEQ WNNIGTWVQR AIIPLKSELV
260 270 280 290 300
MGDGNTGSDI FDGVGFRGVR LYSSDNMYPD SQQGFAPTVR GIARTAAQLT
310 320 330 340 350
IRQNGFIIYQ SYVSPGAFEI TDLHPTSSNG DLDVTIDERD GNQQNYTIPY
360 370 380 390 400
STVPILQREG RFKFDLTAGD FRSGNSQQSS PFFFQGTALG GLPQEFTAYG
410 420 430 440 450
GTQLSANYTA FLLGLGRNLG NWGAVSLDVT HARSQLADAS RHEGDSIRFL
460 470 480 490 500
YAKSMNTFGT NFQLMGYRYS TQGFYTLDDV AYRRMEGYEY DYDGEHRDEP
510 520 530 540 550
IIVNYHNLRF SRKDRLQLNV SQSLNDFGSL YISGTHQKYW NTSDSDTWYQ
560 570 580 590 600
VGYTSSWVGI SYSLSFSWNE SVGIPDNERI VGLNVSVPFN VLTKRRYTRE
610 620 630 640 650
NALDRAYASF NANRNSNGQN SWLAGVGGTL LEGHNLSYHV SQGDTSNNGY
660 670 680 690 700
TGSATANWQA AYGTLGGGYN YDRDQHDVNW QLSGGVVGHE NGITLSQPLG
710 720 730 740 750
DTNVLIKAPG AGGVRIENQT GILTDWRGYA VMLYATVYRY NRIALDTNTM
760 770 780 790 800
GNSIDVEKNI SSVVPTQGAL VRANFDTRIG VRALITVTQG GKPVPFGSLV
810 820 830 840 850
RENSTGITSM VGDDGQVYLS GAPLSGELLV QWGDGANSRC IAHYVLPKQS
860
LQQAVTVISA VCTHPGS
Length:867
Mass (Da):95,677
Last modified:February 1, 1997 - v1
Checksum:iDF8591D0E6C4205A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40730.1.
U82664 Genomic DNA. Translation: AAB40285.1.
U00096 Genomic DNA. Translation: AAC73634.1.
AP009048 Genomic DNA. Translation: BAE76309.1.
PIRiC64785.
RefSeqiNP_415065.1. NC_000913.3.
WP_001333622.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73634; AAC73634; b0532.
BAE76309; BAE76309; BAE76309.
GeneIDi945160.
KEGGiecj:JW0521.
eco:b0532.
PATRICi32116224. VBIEscCol129921_0553.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40730.1.
U82664 Genomic DNA. Translation: AAB40285.1.
U00096 Genomic DNA. Translation: AAC73634.1.
AP009048 Genomic DNA. Translation: BAE76309.1.
PIRiC64785.
RefSeqiNP_415065.1. NC_000913.3.
WP_001333622.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77468.
SMRiP77468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263216. 173 interactors.
IntActiP77468. 2 interactors.
STRINGi511145.b0532.

Proteomic databases

PaxDbiP77468.
PRIDEiP77468.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73634; AAC73634; b0532.
BAE76309; BAE76309; BAE76309.
GeneIDi945160.
KEGGiecj:JW0521.
eco:b0532.
PATRICi32116224. VBIEscCol129921_0553.

Organism-specific databases

EchoBASEiEB3642.
EcoGeneiEG13883. sfmD.

Phylogenomic databases

eggNOGiENOG4105CW0. Bacteria.
COG3188. LUCA.
HOGENOMiHOG000111414.
InParanoidiP77468.
KOiK07354.
OMAiGSASANW.
PhylomeDBiP77468.

Enzyme and pathway databases

BioCyciEcoCyc:G6292-MONOMER.
ECOL316407:JW0521-MONOMER.

Miscellaneous databases

PROiP77468.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSFMD_ECOLI
AccessioniPrimary (citable) accession number: P77468
Secondary accession number(s): P77133, Q2MBP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The operon is cryptic under classical laboratory conditions, but is functional when constitutively expressed.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.