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Protein

Outer membrane usher protein SfmD

Gene

sfmD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Part of the sfmACDHF fimbrial operon. Could contribute to adhesion to various surfaces in specific environmental niches. Increases adhesion to eukaryotic T24 bladder epithelial cells in the absence of fim genes. Probably involved in the export and assembly of fimbrial subunits across the outer membrane.1 Publication

Miscellaneous

The operon is cryptic under classical laboratory conditions, but is functional when constitutively expressed.1 Publication

GO - Molecular functioni

GO - Biological processi

  • pilus assembly Source: GO_Central
  • pilus organization Source: EcoCyc

Keywordsi

Biological processFimbrium biogenesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6292-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane usher protein SfmD
Gene namesi
Name:sfmD
Ordered Locus Names:b0532, JW0521
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13883 sfmD

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the operon under classical laboratory conditions does not result in any major effect on E.coli capacity to form biofilms compared with the wild-type strain.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000000931336 – 867Outer membrane usher protein SfmDAdd BLAST832

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi840 ↔ 862Sequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP77468
PRIDEiP77468

Expressioni

Inductioni

Expression is negatively regulated by H-NS and subjected to cAMP receptor protein (CRP)-mediated catabolite repression.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263216, 173 interactors
IntActiP77468, 2 interactors
STRINGi316385.ECDH10B_0488

Structurei

3D structure databases

ProteinModelPortaliP77468
SMRiP77468
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fimbrial export usher family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CW0 Bacteria
COG3188 LUCA
HOGENOMiHOG000111414
InParanoidiP77468
KOiK07354
OMAiGSASANW
PhylomeDBiP77468

Family and domain databases

InterProiView protein in InterPro
IPR000015 Fimb_usher
IPR018030 Fimbrial_membr_usher_CS
IPR025949 PapC-like_C
IPR025885 PapC_N
IPR037224 PapC_N_sf
PANTHERiPTHR30451 PTHR30451, 1 hit
PfamiView protein in Pfam
PF13953 PapC_C, 1 hit
PF13954 PapC_N, 1 hit
PF00577 Usher, 1 hit
SUPFAMiSSF141729 SSF141729, 1 hit
PROSITEiView protein in PROSITE
PS01151 FIMBRIAL_USHER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIPTTTDIP QRYTWCLAGI CYSSLAILPS FLSYAESYFN PAFLLENGTS
60 70 80 90 100
VADLSRFERG NHQPAGVYRV DLWRNDEFIG SQDIVFESTT ENTGDKSGGL
110 120 130 140 150
MPCFNQVLLE RIGLNSSAFP ELAQQQNNKC INLLKAVPDA TINFDFAAMR
160 170 180 190 200
LNITIPQIAL LSSAHGYIPP EEWDEGIPAL LLNYNFTGNR GNGNDSYFFS
210 220 230 240 250
ELSGINIGPW RLRNNGSWNY FRGNGYHSEQ WNNIGTWVQR AIIPLKSELV
260 270 280 290 300
MGDGNTGSDI FDGVGFRGVR LYSSDNMYPD SQQGFAPTVR GIARTAAQLT
310 320 330 340 350
IRQNGFIIYQ SYVSPGAFEI TDLHPTSSNG DLDVTIDERD GNQQNYTIPY
360 370 380 390 400
STVPILQREG RFKFDLTAGD FRSGNSQQSS PFFFQGTALG GLPQEFTAYG
410 420 430 440 450
GTQLSANYTA FLLGLGRNLG NWGAVSLDVT HARSQLADAS RHEGDSIRFL
460 470 480 490 500
YAKSMNTFGT NFQLMGYRYS TQGFYTLDDV AYRRMEGYEY DYDGEHRDEP
510 520 530 540 550
IIVNYHNLRF SRKDRLQLNV SQSLNDFGSL YISGTHQKYW NTSDSDTWYQ
560 570 580 590 600
VGYTSSWVGI SYSLSFSWNE SVGIPDNERI VGLNVSVPFN VLTKRRYTRE
610 620 630 640 650
NALDRAYASF NANRNSNGQN SWLAGVGGTL LEGHNLSYHV SQGDTSNNGY
660 670 680 690 700
TGSATANWQA AYGTLGGGYN YDRDQHDVNW QLSGGVVGHE NGITLSQPLG
710 720 730 740 750
DTNVLIKAPG AGGVRIENQT GILTDWRGYA VMLYATVYRY NRIALDTNTM
760 770 780 790 800
GNSIDVEKNI SSVVPTQGAL VRANFDTRIG VRALITVTQG GKPVPFGSLV
810 820 830 840 850
RENSTGITSM VGDDGQVYLS GAPLSGELLV QWGDGANSRC IAHYVLPKQS
860
LQQAVTVISA VCTHPGS
Length:867
Mass (Da):95,677
Last modified:February 1, 1997 - v1
Checksum:iDF8591D0E6C4205A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA Translation: AAB40730.1
U82664 Genomic DNA Translation: AAB40285.1
U00096 Genomic DNA Translation: AAC73634.1
AP009048 Genomic DNA Translation: BAE76309.1
PIRiC64785
RefSeqiNP_415065.1, NC_000913.3
WP_001333622.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73634; AAC73634; b0532
BAE76309; BAE76309; BAE76309
GeneIDi945160
KEGGiecj:JW0521
eco:b0532
PATRICifig|1411691.4.peg.1746

Similar proteinsi

Entry informationi

Entry nameiSFMD_ECOLI
AccessioniPrimary (citable) accession number: P77468
Secondary accession number(s): P77133, Q2MBP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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