P77467 (PAAG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable enoyl-CoA hydratase paaG
EC=4.2.1.17

Gene names

Name:	paaG
Synonyms:	ydbT
Ordered Locus Names:	b1394, JW1389

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Could possibly oxidize fatty acids using specific components By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Pathway	Aromatic compound metabolism; phenylacetate degradation.
Induction	Activated by cAMP receptor protein (CRP) and integration host factor (IHF). Inhibited by paaX. Ref.5
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW phenylacetate catabolic process Inferred from mutant phenotype. Source: EcoCyc
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Probable enoyl-CoA hydratase paaG		PRO_0000109331
Natural variations
	Natural variant	121	1	G → C in strain: W.

Sequences

	Sequence		Length	Mass (Da)
	P77467 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: DD7B95B30D88660C	FASTA	262	28,405
	10 20 30 40 50 60 MMEFILSHVE KGVMTLTLNR PERLNSFNDE MHAQLAECLK QVERDDTIRC LLLTGAGRGF 70 80 90 100 110 120 CAGQDLNDRN VDPTGPAPDL GMSVERFYNP LVRRLAKLPK PVICAVNGVA AGAGATLALG 130 140 150 160 170 180 GDIVIAARSA KFVMAFSKLG LIPDCGGTWL LPRVAGRARA MGLALLGNQL SAEQAHEWGM 190 200 210 220 230 240 IWQVVDDETL ADTAQQLARH LATQPTFGLG LIKQAINSAE TNTLDTQLDL ERDYQRLAGR 250 260 SADYREGVSA FLAKRSPQFT GK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway." Ferrandez A., Minambres B., Garcia B., Olivera E.R., Luengo J.M., Garcia J.L., Diaz E. J. Biol. Chem. 273:25974-25986(1998) [PubMed: 9748275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W / ATCC 11105 / DSM 1900.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Transcriptional regulation of the divergent paa catabolic operons for phenylacetic acid degradation in Escherichia coli." Ferrandez A., Garcia J.L., Diaz E. J. Biol. Chem. 275:12214-12222(2000) [PubMed: 10766858] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97452 Genomic DNA. Translation: CAA66096.1. U00096 Genomic DNA. Translation: AAC74476.1. AP009048 Genomic DNA. Translation: BAA15000.1.
PIR	E64890.
RefSeq	NP_415912.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P77467.
SMR	P77467. Positions 4-262.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77467. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002468; EBESCP00000002468; EBESCG00000002011. EBESCT00000017727; EBESCP00000017018; EBESCG00000016783.
GeneID	946263.
GenomeReviews	Gene locus JW1389 in contig AP009048_GR. Gene locus b1394 in contig U00096_GR.
KEGG	ecj:JW1389. eco:b1394.
PATRIC	32118074. VBIEscCol129921_1457.
Organism-specific databases
EchoBASE	EB3504.
EcoGene	EG13741. paaG.
Phylogenomic databases
eggNOG	COG1024.
GeneTree	EBGT00050000009097.
HOGENOM	HBG748731.
OMA	RSDDYRE.
PhylomeDB	P77467.
ProtClustDB	PRK08140.
Enzyme and pathway databases
BioCyc	EcoCyc:G6715-MONOMER. MetaCyc:G6715-MONOMER.
Gene expression databases
Genevestigator	P77467.
Family and domain databases
InterPro	IPR001753. Crotonase_core. IPR011968. PaaB1. [Graphical view]
KO	K01692.
Pfam	PF00378. ECH. 1 hit. [Graphical view]
TIGRFAMs	TIGR02280. PaaB1. 1 hit.
PROSITE	PS00166. ENOYL_COA_HYDRATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAAG_ECOLI

Accession

Primary (citable) accession number: P77467
Secondary accession number(s): O53015

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program