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Protein

Glutaminase 1

Gene

glsA1

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation1 Publication

Kineticsi

  1. KM=7.3 mM for glutamine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei66SubstrateUniRule annotation1
    Binding sitei117SubstrateUniRule annotation1
    Binding sitei161SubstrateUniRule annotation1
    Binding sitei168SubstrateUniRule annotation1
    Binding sitei192SubstrateUniRule annotation1
    Binding sitei244SubstrateUniRule annotation1
    Binding sitei262Substrate; via amide nitrogenUniRule annotation1

    GO - Molecular functioni

    • glutaminase activity Source: EcoCyc

    GO - Biological processi

    • glutamate biosynthetic process Source: GO_Central
    • glutamine catabolic process Source: GO_Central
    • negative regulation of growth Source: EcoliWiki
    • response to acidic pH Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6261-MONOMER.
    ECOL316407:JW0474-MONOMER.
    MetaCyc:G6261-MONOMER.
    BRENDAi3.5.1.2. 2026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminase 1UniRule annotation (EC:3.5.1.2UniRule annotation)
    Gene namesi
    Name:glsA1UniRule annotation
    Synonyms:ybaS
    Ordered Locus Names:b0485, JW0474
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13247. glsA1.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi69K → A: Loss of activity. 1 Publication1
    Mutagenesisi117N → A: Loss of activity. 1 Publication1
    Mutagenesisi160S → A: Loss of activity. 1 Publication1
    Mutagenesisi161E → A: Strongly reduced activity. 1 Publication1
    Mutagenesisi162Q → A: No effect. 1 Publication1
    Mutagenesisi168N → A: Loss of activity. 1 Publication1
    Mutagenesisi192Y → A: Loss of activity. 1 Publication1
    Mutagenesisi244Y → A: Loss of activity. 1 Publication1
    Mutagenesisi260S → A: Reduced activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001106071 – 310Glutaminase 1Add BLAST310

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei294N6-acetyllysineUniRule annotation1 Publication1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP77454.
    PRIDEiP77454.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation1 Publication

    Protein-protein interaction databases

    BioGridi4261985. 15 interactors.
    DIPiDIP-11307N.
    IntActiP77454. 2 interactors.
    STRINGi511145.b0485.

    Structurei

    Secondary structure

    1310
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi4 – 18Combined sources15
    Helixi31 – 34Combined sources4
    Beta strandi42 – 47Combined sources6
    Beta strandi52 – 57Combined sources6
    Helixi65 – 67Combined sources3
    Helixi68 – 80Combined sources13
    Helixi82 – 88Combined sources7
    Helixi101 – 106Combined sources6
    Turni107 – 109Combined sources3
    Helixi117 – 126Combined sources10
    Helixi132 – 147Combined sources16
    Helixi155 – 162Combined sources8
    Helixi166 – 178Combined sources13
    Helixi185 – 195Combined sources11
    Beta strandi198 – 200Combined sources3
    Helixi202 – 213Combined sources12
    Beta strandi216 – 218Combined sources3
    Turni219 – 222Combined sources4
    Helixi228 – 230Combined sources3
    Helixi231 – 241Combined sources11
    Helixi244 – 246Combined sources3
    Helixi247 – 253Combined sources7
    Beta strandi258 – 260Combined sources3
    Beta strandi264 – 270Combined sources7
    Turni271 – 273Combined sources3
    Beta strandi274 – 279Combined sources6
    Helixi290 – 303Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1U60X-ray1.61A/B/C/D1-310[»]
    ProteinModelPortaliP77454.
    SMRiP77454.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP77454.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutaminase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105CSV. Bacteria.
    COG2066. LUCA.
    HOGENOMiHOG000216890.
    InParanoidiP77454.
    KOiK01425.
    OMAiPMNPMVN.
    PhylomeDBiP77454.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P77454-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG
    60 70 80 90 100
    NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS
    110 120 130 140 150
    VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ
    160 170 180 190 200
    VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL
    210 220 230 240 250
    NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW
    260 270 280 290 300
    AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA
    310
    KQLGYNVFKG
    Length:310
    Mass (Da):32,903
    Last modified:February 1, 1997 - v1
    Checksum:i4448BA0549E3C851
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U82664 Genomic DNA. Translation: AAB40239.1.
    U00096 Genomic DNA. Translation: AAC73587.1.
    AP009048 Genomic DNA. Translation: BAE76264.1.
    PIRiD64779.
    RefSeqiNP_415018.1. NC_000913.3.
    WP_000883034.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73587; AAC73587; b0485.
    BAE76264; BAE76264; BAE76264.
    GeneIDi946187.
    KEGGiecj:JW0474.
    eco:b0485.
    PATRICi32116129. VBIEscCol129921_0506.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U82664 Genomic DNA. Translation: AAB40239.1.
    U00096 Genomic DNA. Translation: AAC73587.1.
    AP009048 Genomic DNA. Translation: BAE76264.1.
    PIRiD64779.
    RefSeqiNP_415018.1. NC_000913.3.
    WP_000883034.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1U60X-ray1.61A/B/C/D1-310[»]
    ProteinModelPortaliP77454.
    SMRiP77454.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261985. 15 interactors.
    DIPiDIP-11307N.
    IntActiP77454. 2 interactors.
    STRINGi511145.b0485.

    Proteomic databases

    PaxDbiP77454.
    PRIDEiP77454.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73587; AAC73587; b0485.
    BAE76264; BAE76264; BAE76264.
    GeneIDi946187.
    KEGGiecj:JW0474.
    eco:b0485.
    PATRICi32116129. VBIEscCol129921_0506.

    Organism-specific databases

    EchoBASEiEB3036.
    EcoGeneiEG13247. glsA1.

    Phylogenomic databases

    eggNOGiENOG4105CSV. Bacteria.
    COG2066. LUCA.
    HOGENOMiHOG000216890.
    InParanoidiP77454.
    KOiK01425.
    OMAiPMNPMVN.
    PhylomeDBiP77454.

    Enzyme and pathway databases

    BioCyciEcoCyc:G6261-MONOMER.
    ECOL316407:JW0474-MONOMER.
    MetaCyc:G6261-MONOMER.
    BRENDAi3.5.1.2. 2026.

    Miscellaneous databases

    EvolutionaryTraceiP77454.
    PROiP77454.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLSA1_ECOLI
    AccessioniPrimary (citable) accession number: P77454
    Secondary accession number(s): Q2MBU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: November 2, 2016
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.