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Protein

Multiphosphoryl transfer protein 1

Gene

fryA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multifunctional protein that includes general (non sugar-specific) and sugar-specific components of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FryABC PTS system is involved in fructose transport.1 Publication

Catalytic activityi

Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.By similarity
[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei15Pros-phosphohistidine intermediate; for HPr activityPROSITE-ProRule annotation1
Active sitei298Tele-phosphohistidine intermediate; for PTS EI activityPROSITE-ProRule annotationBy similarity1
Binding sitei405PEPBy similarity1
Binding sitei441PEPBy similarity1
Metal bindingi540MagnesiumBy similarity1
Metal bindingi564MagnesiumBy similarity1
Binding sitei574PEPBy similarity1
Active sitei611Proton donor; for EI activityBy similarity1
Active sitei747Tele-phosphohistidine intermediate; for PTS EIIA activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7246-MONOMER

Protein family/group databases

TCDBi4.A.2.1.11 the pts fructose-mannitol (fru) family

Names & Taxonomyi

Protein namesi
Recommended name:
Multiphosphoryl transfer protein 11 Publication
Short name:
MTP 11 Publication
Alternative name(s):
Triphosphoryl transfer protein 11 Publication
Short name:
TTP 11 Publication
Including the following 3 domains:
Phosphoenolpyruvate-protein phosphotransferase1 Publication (EC:2.7.3.9By similarity)
Alternative name(s):
Phosphotransferase system enzyme I1 Publication
Phosphocarrier protein HPr1 Publication
Short name:
Protein H1 Publication
PTS system fructose-like EIIA component1 Publication (EC:2.7.1.202By similarity)
Alternative name(s):
Fructose-like phosphotransferase enzyme IIA component1 Publication
Gene namesi
Name:fryA
Synonyms:ypdD
Ordered Locus Names:b2383, JW2380
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14151 fryA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001470971 – 831Multiphosphoryl transfer protein 1Add BLAST831

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15Phosphohistidine; by EICurated1
Modified residuei298Phosphohistidine; by autocatalysisCurated1
Modified residuei747Phosphohistidine; by HPrCurated1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP77439
PRIDEiP77439

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceEP0AFG82EBI-545399,EBI-542683

Protein-protein interaction databases

BioGridi4261077, 13 interactors
DIPiDIP-12817N
IntActiP77439, 1 interactor
STRINGi316385.ECDH10B_2548

Structurei

3D structure databases

ProteinModelPortaliP77439
SMRiP77439
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 90HPrPROSITE-ProRule annotationAdd BLAST90
Domaini685 – 828PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST144

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 650PTS EI1 PublicationAdd BLAST532
Regioni563 – 564PEP bindingBy similarity2

Domaini

The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation
In contrast to classical PTS systems, the fructose-like PTS has no requirement for HPr and Enzyme I; FryA combines a IIA domain with an Enzyme I and a HPr domains.Curated

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ3 Bacteria
COG1080 LUCA
COG1925 LUCA
HOGENOMiHOG000122933
InParanoidiP77439
KOiK08483
K11189
K11201
OMAiLHIMLPM
PhylomeDBiP77439

Family and domain databases

CDDicd00367 PTS-HPr_like, 1 hit
Gene3Di1.10.274.10, 1 hit
3.30.1340.10, 1 hit
3.40.930.10, 1 hit
InterProiView protein in InterPro
IPR000032 HPr-like
IPR035895 HPr-like_sf
IPR008279 PEP-util_enz_mobile_dom
IPR000121 PEP_util_C
IPR023151 PEP_util_CS
IPR036637 Phosphohistidine_dom_sf
IPR016152 PTrfase/Anion_transptr
IPR006318 PTS_EI-like
IPR002178 PTS_EIIA_type-2_dom
IPR008731 PTS_EIN
IPR004715 PTS_IIA_fruc
IPR036618 PtsI_HPr-bd_sf
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PfamiView protein in Pfam
PF05524 PEP-utilisers_N, 1 hit
PF00391 PEP-utilizers, 1 hit
PF02896 PEP-utilizers_C, 1 hit
PF00381 PTS-HPr, 1 hit
PF00359 PTS_EIIA_2, 1 hit
SUPFAMiSSF47831 SSF47831, 1 hit
SSF51621 SSF51621, 1 hit
SSF52009 SSF52009, 1 hit
SSF55594 SSF55594, 1 hit
SSF55804 SSF55804, 1 hit
TIGRFAMsiTIGR00848 fruA, 1 hit
TIGR01417 PTS_I_fam, 1 hit
PROSITEiView protein in PROSITE
PS00742 PEP_ENZYMES_2, 1 hit
PS51094 PTS_EIIA_TYPE_2, 1 hit
PS51350 PTS_HPR_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

P77439-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTIQFLCPL PNGLHARPAW ELKEQCSQWQ SEITFINHRQ NAKADAKSSL
60 70 80 90 100
ALIGTGTLFN DSCSLNISGS DEEQARRVLE EYIQVRFIDS DSVQPTQAEL
110 120 130 140 150
TAHPLPRSLS RLNPDLLYGN VLASGVGVGT LTLLQSDSLD SYRAIPASAQ
160 170 180 190 200
DSTRLEHSLA TLAEQLNQQL RERDGESKTI LSAHLSLIQD DEFAGNIRRL
210 220 230 240 250
MTEQHQGLGA AIISNMEQVC AKLSASASDY LRERVSDIRD ISEQLLHITW
260 270 280 290 300
PELKPRNKLV LEKPTILVAE DLTPSQFLSL DLKNLAGMIL EKTGRTSHTL
310 320 330 340 350
ILARASAIPV LSGLPLDAIA RYAGQPAVLD AQCGVLAINP NDAVSGYYQV
360 370 380 390 400
AQTLADKRQK QQAQAAAQLA YSRDNKRIDI AANIGTALEA PGAFANGAEG
410 420 430 440 450
VGLFRTEMLY MDRDSAPDEQ EQFEAYQQVL LAAGDKPIIF RTMDIGGDKS
460 470 480 490 500
IPYLNIPQEE NPFLGYRAVR IYPEFAGLFR TQLRAILRAA SFGNAQLMIP
510 520 530 540 550
MVHSLDQILW VKGEIQKAIV ELKRDGLRHA ETITLGIMVE VPSVCYIIDH
560 570 580 590 600
FCDEVDFFSI GSNDMTQYLY AVDRNNPRVS PLYNPITPSF LRMLQQIVTT
610 620 630 640 650
AHQRGKWVGI CGELGGESRY LPLLLGLGLD ELSMSSPRIP AVKSQLRQLD
660 670 680 690 700
SEACRELARQ ACECRSAQEI EALLTAFTPE EDVRPLLALE NIFVDQDFSN
710 720 730 740 750
KEQAIQFLCG NLGVNGRTEH PFELEEDVWQ REEIVTTGVG FGVAIPHTKS
760 770 780 790 800
QWIRHSSISI ARLAKPIGWQ SEMGEVELVI MLTLGANEGM NHVKVFSQLA
810 820 830
RKLVNKNFRQ SLFAAQDAQS ILTLLETELT F
Length:831
Mass (Da):92,130
Last modified:February 1, 1997 - v1
Checksum:iB9F3E3B6D4EAB597
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75442.1
AP009048 Genomic DNA Translation: BAA16253.1
PIRiD65012
RefSeqiNP_416884.1, NC_000913.3
WP_000955906.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75442; AAC75442; b2383
BAA16253; BAA16253; BAA16253
GeneIDi946852
KEGGiecj:JW2380
eco:b2383
PATRICifig|1411691.4.peg.4345

Similar proteinsi

Entry informationi

Entry nameiPTFX1_ECOLI
AccessioniPrimary (citable) accession number: P77439
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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