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Protein

Autoinducer-2 kinase

Gene

lsrK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of autoinducer-2 (AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon. Phosphorylates the ring-open form of (S)-4,5-dihydroxypentane-2,3-dione (DPD), which is the precursor to all AI-2 signaling molecules, at the C5 position. Required for the regulation of the lsr operon and many other genes.3 Publications

Catalytic activityi

ATP + (S)-4,5-dihydroxypentane-2,3-dione = ADP + (S)-4-hydroxy-2,3-pentanedione 5-phosphate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • quorum sensing Source: EcoCyc
  • single-species biofilm formation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G6798-MONOMER.
ECOL316407:JW1504-MONOMER.
MetaCyc:G6798-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autoinducer-2 kinaseUniRule annotation (EC:2.7.1.189UniRule annotation)
Short name:
AI-2 kinaseUniRule annotation
Gene namesi
Name:lsrKUniRule annotation
Synonyms:ydeV
Ordered Locus Names:b1511, JW1504
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13804. lsrK.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Autoinducer-2 kinasePRO_0000059566Add
BLAST

Proteomic databases

PaxDbiP77432.

Interactioni

Protein-protein interaction databases

BioGridi4260224. 9 interactions.
DIPiDIP-11687N.
IntActiP77432. 13 interactions.
MINTiMINT-1251018.
STRINGi511145.b1511.

Structurei

3D structure databases

ProteinModelPortaliP77432.
SMRiP77432. Positions 11-513.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108EMV. Bacteria.
COG1070. LUCA.
HOGENOMiHOG000092677.
InParanoidiP77432.
KOiK11216.
OMAiFSDAMHF.
PhylomeDBiP77432.

Family and domain databases

HAMAPiMF_02053. LsrK. 1 hit.
InterProiIPR033676. AI-2_kinase.
IPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018484. Carb_kinase_FGGY_N.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.

Sequencei

Sequence statusi: Complete.

P77432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLFTLSES KYYLMALDAG TGSIRAVIFD LEGNQIAVGQ AEWRHLAVPD
60 70 80 90 100
VPGSMEFDLN KNWQLACECM RQALHNAGIA PEYIAAVSAC SMREGIVLYN
110 120 130 140 150
NEGAPIWACA NVDARAAREV SELKELHNNT FENEVYRATG QTLALSAIPR
160 170 180 190 200
LLWLAHHRSD IYRQASTITM ISDWLAYMLS GELAVDPSNA GTTGLLDLTT
210 220 230 240 250
RDWKPALLDM AGLRADILSP VKETGTLLGV VSSQAAELCG LKAGTPVVVG
260 270 280 290 300
GGDVQLGCLG LGVVRPAQTA VLGGTFWQQV VNLAAPVTDP EMNVRVNPHV
310 320 330 340 350
IPGMVQAESI SFFTGLTMRW FRDAFCAEEK LIAERLGIDT YTLLEEMASR
360 370 380 390 400
VPPGSWGVMP IFSDRMRFKT WYHAAPSFIN LSIDPDKCNK ATLFRALEEN
410 420 430 440 450
AAIVSACNLQ QIADFSNIHP SSLVFAGGGS KGKLWSQILA DVSGLPVNIP
460 470 480 490 500
VVKEATALGC AIAAGVGAGI FSSMAETGER LVRWERTHTP DPEKHELYQD
510 520 530
SRDKWQAVYQ DQLGLVDHGL TTSLWKAPGL
Length:530
Mass (Da):57,545
Last modified:February 1, 1997 - v1
Checksum:iCBC3B1E7C8982063
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti490 – 4956PDPEKH → TRPGKA (PubMed:9097039).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74584.1.
AP009048 Genomic DNA. Translation: BAA15191.1.
S82185 mRNA. Translation: AAC17184.1.
PIRiB64905.
RefSeqiNP_416028.3. NC_000913.3.
WP_000113108.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74584; AAC74584; b1511.
BAA15191; BAA15191; BAA15191.
GeneIDi946069.
KEGGiecj:JW1504.
eco:b1511.
PATRICi32118318. VBIEscCol129921_1579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74584.1.
AP009048 Genomic DNA. Translation: BAA15191.1.
S82185 mRNA. Translation: AAC17184.1.
PIRiB64905.
RefSeqiNP_416028.3. NC_000913.3.
WP_000113108.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77432.
SMRiP77432. Positions 11-513.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260224. 9 interactions.
DIPiDIP-11687N.
IntActiP77432. 13 interactions.
MINTiMINT-1251018.
STRINGi511145.b1511.

Proteomic databases

PaxDbiP77432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74584; AAC74584; b1511.
BAA15191; BAA15191; BAA15191.
GeneIDi946069.
KEGGiecj:JW1504.
eco:b1511.
PATRICi32118318. VBIEscCol129921_1579.

Organism-specific databases

EchoBASEiEB3565.
EcoGeneiEG13804. lsrK.

Phylogenomic databases

eggNOGiENOG4108EMV. Bacteria.
COG1070. LUCA.
HOGENOMiHOG000092677.
InParanoidiP77432.
KOiK11216.
OMAiFSDAMHF.
PhylomeDBiP77432.

Enzyme and pathway databases

BioCyciEcoCyc:G6798-MONOMER.
ECOL316407:JW1504-MONOMER.
MetaCyc:G6798-MONOMER.

Miscellaneous databases

PROiP77432.

Family and domain databases

HAMAPiMF_02053. LsrK. 1 hit.
InterProiIPR033676. AI-2_kinase.
IPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018484. Carb_kinase_FGGY_N.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLSRK_ECOLI
AccessioniPrimary (citable) accession number: P77432
Secondary accession number(s): Q99894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to originate from human and was called BRAG1 (brain-related apoptosis gene 1) with a proposed role in apoptosis (PubMed:8649811). The DNA sequence of the region sequenced is more than 99% identical to that of this E.coli gene. Furthermore the claim of an 'extensive similarity to the Bcl-2 family of genes' is not correct.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.