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Protein

Autoinducer-2 kinase

Gene

lsrK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of autoinducer-2 (AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon. Phosphorylates the ring-open form of (S)-4,5-dihydroxypentane-2,3-dione (DPD), which is the precursor to all AI-2 signaling molecules, at the C5 position. Required for the regulation of the lsr operon and many other genes.3 Publications

Caution

Was originally thought to originate from human and was called BRAG1 (brain-related apoptosis gene 1) with a proposed role in apoptosis (PubMed:8649811). The DNA sequence of the region sequenced is more than 99% identical to that of this E.coli gene. Furthermore the claim of an 'extensive similarity to the Bcl-2 family of genes' is not correct.1 Publication

Catalytic activityi

ATP + (S)-4,5-dihydroxypentane-2,3-dione = ADP + (S)-4-hydroxy-2,3-pentanedione 5-phosphate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • quorum sensing Source: EcoCyc
  • single-species biofilm formation Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G6798-MONOMER
MetaCyc:G6798-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Autoinducer-2 kinaseUniRule annotation (EC:2.7.1.189UniRule annotation)
Short name:
AI-2 kinaseUniRule annotation
Gene namesi
Name:lsrKUniRule annotation
Synonyms:ydeV
Ordered Locus Names:b1511, JW1504
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG13804 lsrK

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000595661 – 530Autoinducer-2 kinaseAdd BLAST530

Proteomic databases

PaxDbiP77432
PRIDEiP77432

Interactioni

Protein-protein interaction databases

BioGridi4260224, 14 interactors
DIPiDIP-11687N
IntActiP77432, 13 interactors
STRINGi316385.ECDH10B_1642

Structurei

3D structure databases

ProteinModelPortaliP77432
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108EMV Bacteria
COG1070 LUCA
HOGENOMiHOG000092677
InParanoidiP77432
KOiK11216
OMAiFSDAMHF
PhylomeDBiP77432

Family and domain databases

HAMAPiMF_02053 LsrK, 1 hit
InterProiView protein in InterPro
IPR033676 AI-2_kinase
IPR000577 Carb_kinase_FGGY
IPR018485 Carb_kinase_FGGY_C
IPR018484 Carb_kinase_FGGY_N
PfamiView protein in Pfam
PF02782 FGGY_C, 1 hit
PF00370 FGGY_N, 1 hit
PIRSFiPIRSF000538 GlpK, 1 hit

Sequencei

Sequence statusi: Complete.

P77432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLFTLSES KYYLMALDAG TGSIRAVIFD LEGNQIAVGQ AEWRHLAVPD
60 70 80 90 100
VPGSMEFDLN KNWQLACECM RQALHNAGIA PEYIAAVSAC SMREGIVLYN
110 120 130 140 150
NEGAPIWACA NVDARAAREV SELKELHNNT FENEVYRATG QTLALSAIPR
160 170 180 190 200
LLWLAHHRSD IYRQASTITM ISDWLAYMLS GELAVDPSNA GTTGLLDLTT
210 220 230 240 250
RDWKPALLDM AGLRADILSP VKETGTLLGV VSSQAAELCG LKAGTPVVVG
260 270 280 290 300
GGDVQLGCLG LGVVRPAQTA VLGGTFWQQV VNLAAPVTDP EMNVRVNPHV
310 320 330 340 350
IPGMVQAESI SFFTGLTMRW FRDAFCAEEK LIAERLGIDT YTLLEEMASR
360 370 380 390 400
VPPGSWGVMP IFSDRMRFKT WYHAAPSFIN LSIDPDKCNK ATLFRALEEN
410 420 430 440 450
AAIVSACNLQ QIADFSNIHP SSLVFAGGGS KGKLWSQILA DVSGLPVNIP
460 470 480 490 500
VVKEATALGC AIAAGVGAGI FSSMAETGER LVRWERTHTP DPEKHELYQD
510 520 530
SRDKWQAVYQ DQLGLVDHGL TTSLWKAPGL
Length:530
Mass (Da):57,545
Last modified:February 1, 1997 - v1
Checksum:iCBC3B1E7C8982063
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti490 – 495PDPEKH → TRPGKA (PubMed:9097039).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74584.1
AP009048 Genomic DNA Translation: BAA15191.1
S82185 mRNA Translation: AAC17184.1
PIRiB64905
RefSeqiNP_416028.3, NC_000913.3
WP_000113108.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74584; AAC74584; b1511
BAA15191; BAA15191; BAA15191
GeneIDi946069
KEGGiecj:JW1504
eco:b1511
PATRICifig|1411691.4.peg.756

Similar proteinsi

Entry informationi

Entry nameiLSRK_ECOLI
AccessioniPrimary (citable) accession number: P77432
Secondary accession number(s): Q99894
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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