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Protein

Hydrogenase-4 component H

Gene

hyfH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Probable electron transfer protein for hydrogenase 4.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi43 – 431Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi46 – 461Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi50 – 501Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi75 – 751Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi78 – 781Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi81 – 811Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi85 – 851Iron-sulfur 2 (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-144.
ECOL316407:JW2473-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-4 component H
Gene namesi
Name:hyfH
Ordered Locus Names:b2488, JW2473
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14216. hyfH.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Hydrogenase-4 component HPRO_0000159268Add
BLAST

Proteomic databases

PaxDbiP77423.

Interactioni

Protein-protein interaction databases

BioGridi4261172. 17 interactions.
STRINGi511145.b2488.

Structurei

3D structure databases

ProteinModelPortaliP77423.
SMRiP77423. Positions 15-106.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 60304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini66 – 95304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1143. LUCA.
HOGENOMiHOG000278697.
InParanoidiP77423.
KOiK12143.
OMAiMACPANA.
OrthoDBiEOG6XM7H5.
PhylomeDBiP77423.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKLLKTIMR AGTATVKYPF APLEVSPGFR GKPDLMPSQC IACGACACAC
60 70 80 90 100
PANALTIQTD DQQNSRTWQL YLGRCIYCGR CEEVCPTRAI QLTNNFELTV
110 120 130 140 150
TNKADLYTRA TFHLQRCSRC ERPFAPQKTI ALAAELLAQQ QNAPQNREML
160 170 180
WAQASVCPEC KQRATLINDD TDVLLVAKEQ L
Length:181
Mass (Da):20,159
Last modified:February 1, 1997 - v1
Checksum:i5DBBA3C7C7EBD885
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88570.1.
U00096 Genomic DNA. Translation: AAC75541.1.
AP009048 Genomic DNA. Translation: BAA16376.1.
PIRiG65024.
RefSeqiNP_416983.1. NC_000913.3.
WP_000916055.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75541; AAC75541; b2488.
BAA16376; BAA16376; BAA16376.
GeneIDi946965.
KEGGiecj:JW2473.
eco:b2488.
PATRICi32120363. VBIEscCol129921_2584.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88570.1.
U00096 Genomic DNA. Translation: AAC75541.1.
AP009048 Genomic DNA. Translation: BAA16376.1.
PIRiG65024.
RefSeqiNP_416983.1. NC_000913.3.
WP_000916055.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77423.
SMRiP77423. Positions 15-106.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261172. 17 interactions.
STRINGi511145.b2488.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP77423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75541; AAC75541; b2488.
BAA16376; BAA16376; BAA16376.
GeneIDi946965.
KEGGiecj:JW2473.
eco:b2488.
PATRICi32120363. VBIEscCol129921_2584.

Organism-specific databases

EchoBASEiEB3968.
EcoGeneiEG14216. hyfH.

Phylogenomic databases

eggNOGiCOG1143. LUCA.
HOGENOMiHOG000278697.
InParanoidiP77423.
KOiK12143.
OMAiMACPANA.
OrthoDBiEOG6XM7H5.
PhylomeDBiP77423.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-144.
ECOL316407:JW2473-MONOMER.

Miscellaneous databases

PROiP77423.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Andrews S.C., McClay J., Ambler A., Quail M., Berks B.C., Guest J.R.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiHYFH_ECOLI
AccessioniPrimary (citable) accession number: P77423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.