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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long-chain fatty acids.2 Publications

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei118Important for catalytic activityBy similarity1
Sitei140Important for catalytic activityBy similarity1

GO - Molecular functioni

  • 3-hydroxyacyl-CoA dehydrogenase activity Source: EcoCyc
  • 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-EC
  • enoyl-CoA hydratase activity Source: EcoCyc
  • NAD binding Source: InterPro

GO - Biological processi

  • fatty acid beta-oxidation Source: EcoCyc

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:G7212-MONOMER
MetaCyc:G7212-MONOMER
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:fadJ
Synonyms:yfcX
Ordered Locus Names:b2341, JW2338
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14127 fadJ

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001092981 – 714Fatty acid oxidation complex subunit alphaAdd BLAST714

Proteomic databases

EPDiP77399
PaxDbiP77399
PRIDEiP77399

Expressioni

Inductioni

Unlike the aerobic pathway, the anaerobic pathway is not strongly repressed by FadR regulatory protein.

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
yfdHP772932EBI-545361,EBI-545379

Protein-protein interaction databases

BioGridi4260533252 interactors.
DIPiDIP-11990N
IntActiP77399 8 interactors.
STRINGi316385.ECDH10B_2504

Structurei

3D structure databases

ProteinModelPortaliP77399
SMRiP77399
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190Enoyl-CoA hydrataseAdd BLAST190
Regioni306 – 7143-hydroxyacyl-CoA dehydrogenaseAdd BLAST409

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261346
InParanoidiP77399
KOiK01782
OMAiPFRYMDT
PhylomeDBiP77399

Family and domain databases

HAMAPiMF_01617 FadJ, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012802 FadJ
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02440 FadJ, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

P77399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMTSAFTLN VRLDNIAVIT IDVPGEKMNT LKAEFASQVR AIIKQLRENK
60 70 80 90 100
ELRGVVFVSA KPDNFIAGAD INMIGNCKTA QEAEALARQG QQLMAEIHAL
110 120 130 140 150
PIQVIAAIHG ACLGGGLELA LACHGRVCTD DPKTVLGLPE VQLGLLPGSG
160 170 180 190 200
GTQRLPRLIG VSTALEMILT GKQLRAKQAL KLGLVDDVVP HSILLEAAVE
210 220 230 240 250
LAKKERPSSR PLPVRERILA GPLGRALLFK MVGKKTEHKT QGNYPATERI
260 270 280 290 300
LEVVETGLAQ GTSSGYDAEA RAFGELAMTP QSQALRSIFF ASTDVKKDPG
310 320 330 340 350
SDAPPAPLNS VGILGGGLMG GGIAYVTACK AGIPVRIKDI NPQGINHALK
360 370 380 390 400
YSWDQLEGKV RRRHLKASER DKQLALISGT TDYRGFAHRD LIIEAVFENL
410 420 430 440 450
ELKQQMVAEV EQNCAAHTIF ASNTSSLPIG DIAAHATRPE QVIGLHFFSP
460 470 480 490 500
VEKMPLVEII PHAGTSAQTI ATTVKLAKKQ GKTPIVVRDK AGFYVNRILA
510 520 530 540 550
PYINEAIRML TQGERVEHID AALVKFGFPV GPIQLLDEVG IDTGTKIIPV
560 570 580 590 600
LEAAYGERFS APANVVSSIL NDDRKGRKNG RGFYLYGQKG RKSKKQVDPA
610 620 630 640 650
IYPLIGTQGQ GRISAPQVAE RCVMLMLNEA VRCVDEQVIR SVRDGDIGAV
660 670 680 690 700
FGIGFPPFLG GPFRYIDSLG AGEVVAIMQR LATQYGSRFT PCERLVEMGA
710
RGESFWKTTA TDLQ
Length:714
Mass (Da):77,072
Last modified:February 1, 1997 - v1
Checksum:iF4E0A75680039A0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75401.1
AP009048 Genomic DNA Translation: BAA16195.1
PIRiC65007
RefSeqiNP_416843.1, NC_000913.3
WP_000426176.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75401; AAC75401; b2341
BAA16195; BAA16195; BAA16195
GeneIDi949097
KEGGiecj:JW2338
eco:b2341
PATRICifig|1411691.4.peg.4391

Similar proteinsi

Entry informationi

Entry nameiFADJ_ECOLI
AccessioniPrimary (citable) accession number: P77399
Secondary accession number(s): Q6KCX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome