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Protein

3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase

Gene

mhpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI).1 Publication

Catalytic activityi

3-(3-hydroxyphenyl)propanoate + NADH + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+.
(2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+.

Cofactori

FADBy similarity

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 4630FADSequence analysisAdd
BLAST
Nucleotide bindingi285 – 29511FADSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciEcoCyc:MHPHYDROXY-MONOMER.
ECOL316407:JW0338-MONOMER.
MetaCyc:MHPHYDROXY-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (EC:1.14.13.127)
Short name:
3-HCI hydroxylase
Short name:
3-HPP hydroxylase
Gene namesi
Name:mhpA
Ordered Locus Names:b0347, JW0338
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20273. mhpA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5545543-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylasePRO_0000214043Add
BLAST

Proteomic databases

PaxDbiP77397.
PRIDEiP77397.

Interactioni

Protein-protein interaction databases

BioGridi4260728. 14 interactions.
IntActiP77397. 3 interactions.
STRINGi511145.b0347.

Structurei

3D structure databases

ProteinModelPortaliP77397.
SMRiP77397. Positions 18-419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PheA/TfdB FAD monooxygenase family.Curated

Phylogenomic databases

eggNOGiENOG4105E2Q. Bacteria.
COG0654. LUCA.
HOGENOMiHOG000221401.
InParanoidiP77397.
KOiK05712.
OMAiRRVYTHH.
PhylomeDBiP77397.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01652. MhpA. 1 hit.
InterProiIPR023786. 3-HPP/3HCI_hydroxylase.
IPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

P77397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIQHPDIQP AVNHSVQVAI AGAGPVGLMM ANYLGQMGID VLVVEKLDKL
60 70 80 90 100
IDYPRAIGID DEALRTMQSV GLVDDVLPHT TPWHAMRFLT PKGRCFADIQ
110 120 130 140 150
PMTDEFGWPR RNAFIQPQVD AVMLEGVSRF PNVRCLFSRE LEAFSQQDDE
160 170 180 190 200
VTLHLKTAEG QREIVKAQWL VACDGGASFV RRTLNVPFEG KTAPNQWIVV
210 220 230 240 250
DIANDPLSTP HIYLCCDPVR PYVSAALPHA VRRFEFMVMP GETEEQLREP
260 270 280 290 300
QNMRKLLSKV LPNPDNVELI RQRVYTHNAR LAQRFRIDRV LLAGDAAHIM
310 320 330 340 350
PVWQGQGYNS GMRDAFNLAW KLALVIQGKA RDALLDTYQQ ERRDHAKAMI
360 370 380 390 400
DLSVTAGNVL APPKRWQGTL RDGVSWLLNY LPPVKRYFLE MRFKPMPQYY
410 420 430 440 450
GGALMREGEA KHSPVGKMFI QPKVTLENGD VTLLDNAIGA NFAVIGWGCN
460 470 480 490 500
PLWGMSDEQI QQWRALGTRF IQVVPEVQIH TAQDNHDGVL RVGDTQGRLR
510 520 530 540 550
SWFAQHNASL VVMRPDRFVA ATAIPQTLGK TLNKLASVMT LTRPDADVSV

EKVA
Length:554
Mass (Da):62,186
Last modified:February 1, 1997 - v1
Checksum:i1D56CB799E9F8A8E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti272 – 2721Q → H in BAA13052 (Ref. 1) Curated
Sequence conflicti360 – 3601L → P in BAA13052 (Ref. 1) Curated
Sequence conflicti502 – 5021W → G in BAA13052 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA. Translation: BAA13052.1.
Y09555 Genomic DNA. Translation: CAA70747.1.
U73857 Genomic DNA. Translation: AAB18071.1.
U00096 Genomic DNA. Translation: AAC73450.1.
AP009048 Genomic DNA. Translation: BAE76129.1.
PIRiC64762.
RefSeqiNP_414881.1. NC_000913.3.
WP_001007407.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73450; AAC73450; b0347.
BAE76129; BAE76129; BAE76129.
GeneIDi945197.
KEGGiecj:JW0338.
eco:b0347.
PATRICi32115827. VBIEscCol129921_0355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA. Translation: BAA13052.1.
Y09555 Genomic DNA. Translation: CAA70747.1.
U73857 Genomic DNA. Translation: AAB18071.1.
U00096 Genomic DNA. Translation: AAC73450.1.
AP009048 Genomic DNA. Translation: BAE76129.1.
PIRiC64762.
RefSeqiNP_414881.1. NC_000913.3.
WP_001007407.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77397.
SMRiP77397. Positions 18-419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260728. 14 interactions.
IntActiP77397. 3 interactions.
STRINGi511145.b0347.

Proteomic databases

PaxDbiP77397.
PRIDEiP77397.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73450; AAC73450; b0347.
BAE76129; BAE76129; BAE76129.
GeneIDi945197.
KEGGiecj:JW0338.
eco:b0347.
PATRICi32115827. VBIEscCol129921_0355.

Organism-specific databases

EchoBASEiEB4166.
EcoGeneiEG20273. mhpA.

Phylogenomic databases

eggNOGiENOG4105E2Q. Bacteria.
COG0654. LUCA.
HOGENOMiHOG000221401.
InParanoidiP77397.
KOiK05712.
OMAiRRVYTHH.
PhylomeDBiP77397.

Enzyme and pathway databases

UniPathwayiUPA00714.
BioCyciEcoCyc:MHPHYDROXY-MONOMER.
ECOL316407:JW0338-MONOMER.
MetaCyc:MHPHYDROXY-MONOMER.

Miscellaneous databases

PROiP77397.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01652. MhpA. 1 hit.
InterProiIPR023786. 3-HPP/3HCI_hydroxylase.
IPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiMHPA_ECOLI
AccessioniPrimary (citable) accession number: P77397
Secondary accession number(s): P71203, P77047, Q2MC77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.