P77397 (MHPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 88.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase Short name=3-HCI hydroxylase Short name=3-HPP hydroxylase EC=1.14.13.127 | ||||
| Gene names |
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| Organism | Escherichia coli (strain K12) | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 554 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI). Ref.6 |
| Catalytic activity | 3-(3-hydroxyphenyl)propanoate + NADH + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+. HAMAP MF_01652 (2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+. HAMAP MF_01652 |
| Cofactor | FAD By similarity. HAMAP MF_01652 |
| Pathway | Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01652 |
| Sequence similarities | Belongs to the pheA/tfdB FAD monooxygenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Aromatic hydrocarbons catabolism |
| Ligand | FAD Flavoprotein NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | 3-(3-hydroxy)phenylpropionate catabolic process Inferred from electronic annotation. Source: InterPro aromatic compound catabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 3-(3-hydroxyphenyl)propionate hydroxylase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 554 | 554 | 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase HAMAP MF_01652 | PRO_0000214043 | |||||
Regions | |||||||||
| Nucleotide binding | 17 – 46 | 30 | FAD Potential | ||||||
| Nucleotide binding | 285 – 295 | 11 | FAD Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 272 | 1 | Q → H in BAA13052. Ref.1 | ||||||
| Sequence conflict | 360 | 1 | L → P in BAA13052. Ref.1 | ||||||
| Sequence conflict | 502 | 1 | W → G in BAA13052. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Kawamukai M. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [2] | "Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12." Ferrandez A., Garcia J.L., Diaz E. J. Bacteriol. 179:2573-2581(1997) [PubMed: 9098055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / CS520. |
| [3] | "Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [5] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [6] | "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12." Diaz E., Ferrandez A., Garcia J.L. J. Bacteriol. 180:2915-2923(1998) [PubMed: 9603882] [Abstract] Cited for: FUNCTION IN CATABOLISM OF 3-HYDROXY DERIVATIVES OF PHENYLPROPIONIC ACID. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D86239 Genomic DNA. Translation: BAA13052.1. Y09555 Genomic DNA. Translation: CAA70747.1. U73857 Genomic DNA. Translation: AAB18071.1. U00096 Genomic DNA. Translation: AAC73450.1. AP009048 Genomic DNA. Translation: BAE76129.1. |
| PIR | C64762. |
| RefSeq | NP_414881.1. NC_000913.2. |
3D structure databases | |
| ProteinModelPortal | P77397. |
| SMR | P77397. Positions 13-540. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P77397. 2 interactions. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000000784; EBESCP00000000784; EBESCG00000000654. EBESCT00000017328; EBESCP00000016619; EBESCG00000016384. |
| GeneID | 945197. |
| GenomeReviews | Gene locus JW0338 in contig AP009048_GR. Gene locus b0347 in contig U00096_GR. |
| KEGG | ecj:JW0338. eco:b0347. |
| PATRIC | 32115827. VBIEscCol129921_0355. |
Organism-specific databases | |
| EchoBASE | EB4166. |
| EcoGene | EG20273. mhpA. |
Phylogenomic databases | |
| eggNOG | COG0654. |
| GeneTree | EBGT00050000008990. |
| HOGENOM | HBG565573. |
| OMA | WSRRNAF. |
| PhylomeDB | P77397. |
| ProtClustDB | PRK06183. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:MHPHYDROXY-MONOMER. MetaCyc:MHPHYDROXY-MONOMER. |
Gene expression databases | |
| Genevestigator | P77397. |
Family and domain databases | |
| HAMAP | MF_01652. MhpA. [Tree] |
| InterPro | IPR023786. 3-HPP/3HCI_hydroxylase. IPR002938. mOase_FAD-bd. IPR003042. Rng_hydrolase-like. [Graphical view] |
| KO | K05712. |
| Pfam | PF01494. FAD_binding_3. 1 hit. [Graphical view] |
| PRINTS | PR00420. RNGMNOXGNASE. |
| ProtoNet | Search... |
Entry information
| Entry name | MHPA_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P77397 Secondary accession number(s): P71203, P77047, Q2MC77 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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