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Protein

RCS-specific HTH-type transcriptional activator RclR

Gene

rclR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in reactive chlorine species (RCS) stress resistance. Upregulates, in response to hypochlorous acid (HOCl), the expression of three genes essential for survival of RCS stress (rclA, rclB and rclC) and its own expression.1 Publication

Enzyme regulationi

Oxydation of Cys-21 leads to partial activation of RclR, followed by the formation of an intramolecular disulfide bond between Cys-21 and Cys-89, which stabilizes the active form of RclR.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi197 – 21822H-T-H motifPROSITE-ProRule annotationAdd
BLAST
DNA bindingi245 – 26824H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • regulation of cellular organohalogen metabolic process Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6175-MONOMER.
ECOL316407:JW0298-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RCS-specific HTH-type transcriptional activator RclR
Alternative name(s):
Reactive chlorine resistance protein R
Gene namesi
Name:rclR
Synonyms:ykgD
Ordered Locus Names:b0305, JW0298
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13581. rclR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mutants are more sensitive to HOCl treatment than wild-type cells.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi21 – 211C → A: Does not induce expression of target genes in response to HOCl. 1 Publication
Mutagenesisi42 – 421H → A: Increases expression of the target genes in the absence of HOCl. 1 Publication
Mutagenesisi75 – 751H → A: Increases expression of the target genes in the absence of HOCl. In vitro, increases DNA binding under reducing conditions. 1 Publication
Mutagenesisi89 – 891C → A: Does not induce expression of target genes in response to HOCl. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284RCS-specific HTH-type transcriptional activator RclRPRO_0000194613Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi21 ↔ 891 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP77379.
PRIDEiP77379.

Expressioni

Inductioni

Autoregulated. Induced in response to HOCl.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262810. 9 interactions.
IntActiP77379. 5 interactions.
STRINGi511145.b0305.

Structurei

3D structure databases

ProteinModelPortaliP77379.
SMRiP77379. Positions 191-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini177 – 278102HTH araC/xylS-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108K01. Bacteria.
COG2207. LUCA.
HOGENOMiHOG000136816.
InParanoidiP77379.
OMAiCMDFELG.
OrthoDBiEOG690MBW.
PhylomeDBiP77379.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
InterProiIPR032783. AraC_lig.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12852. Cupin_6. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51182. SSF51182. 1 hit.
PROSITEiPS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77379-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALSRLLML NAPQGTIDKN CVLGSDWQLP HGAGELSVIR WHALTQGAAK
60 70 80 90 100
LEMPTGEIFT LRPGNVVLLP QNSAHRLSHV DNESTCIVCG TLRLQHSARY
110 120 130 140 150
FLTSLPETLF LAPVNHSVEY NWLREAIPFL QQESRSAMPG VDALCSQICA
160 170 180 190 200
TFFTLAVREW IAQVNTEKNI LSLLLHPRLG AVIQQMLEMP GHAWTVESLA
210 220 230 240 250
SIAHMSRASF AQLFRDVSGT TPLAVLTKLR LQIAAQMFSR ETLPVVVIAE
260 270 280
SVGYASESSF HKAFVREFGC TPGEYRERVR QLAP
Length:284
Mass (Da):31,474
Last modified:February 1, 1997 - v1
Checksum:i1896705347276040
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18032.1.
U00096 Genomic DNA. Translation: AAC73408.1.
AP009048 Genomic DNA. Translation: BAE76089.1.
PIRiA64757.
RefSeqiNP_414839.1. NC_000913.3.
WP_000339594.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73408; AAC73408; b0305.
BAE76089; BAE76089; BAE76089.
GeneIDi945616.
KEGGiecj:JW0298.
eco:b0305.
PATRICi32115739. VBIEscCol129921_0312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18032.1.
U00096 Genomic DNA. Translation: AAC73408.1.
AP009048 Genomic DNA. Translation: BAE76089.1.
PIRiA64757.
RefSeqiNP_414839.1. NC_000913.3.
WP_000339594.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77379.
SMRiP77379. Positions 191-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262810. 9 interactions.
IntActiP77379. 5 interactions.
STRINGi511145.b0305.

Proteomic databases

PaxDbiP77379.
PRIDEiP77379.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73408; AAC73408; b0305.
BAE76089; BAE76089; BAE76089.
GeneIDi945616.
KEGGiecj:JW0298.
eco:b0305.
PATRICi32115739. VBIEscCol129921_0312.

Organism-specific databases

EchoBASEiEB3351.
EcoGeneiEG13581. rclR.

Phylogenomic databases

eggNOGiENOG4108K01. Bacteria.
COG2207. LUCA.
HOGENOMiHOG000136816.
InParanoidiP77379.
OMAiCMDFELG.
OrthoDBiEOG690MBW.
PhylomeDBiP77379.

Enzyme and pathway databases

BioCyciEcoCyc:G6175-MONOMER.
ECOL316407:JW0298-MONOMER.

Miscellaneous databases

PROiP77379.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
InterProiIPR032783. AraC_lig.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12852. Cupin_6. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51182. SSF51182. 1 hit.
PROSITEiPS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The RclR protein is a reactive chlorine-specific transcription factor in Escherichia coli."
    Parker B.W., Schwessinger E.A., Jakob U., Gray M.J.
    J. Biol. Chem. 288:32574-32584(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, ENZYME REGULATION, DISULFIDE BOND, INDUCTION, DISRUPTION PHENOTYPE, GENE NAME, MUTAGENESIS OF CYS-21; HIS-42; HIS-75 AND CYS-89.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiRCLR_ECOLI
AccessioniPrimary (citable) accession number: P77379
Secondary accession number(s): Q2MCB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.