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Protein

RCS-specific HTH-type transcriptional activator RclR

Gene

rclR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in reactive chlorine species (RCS) stress resistance. Upregulates, in response to hypochlorous acid (HOCl), the expression of three genes essential for survival of RCS stress (rclA, rclB and rclC) and its own expression.1 Publication

Enzyme regulationi

Oxydation of Cys-21 leads to partial activation of RclR, followed by the formation of an intramolecular disulfide bond between Cys-21 and Cys-89, which stabilizes the active form of RclR.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi197 – 218H-T-H motifPROSITE-ProRule annotationAdd BLAST22
DNA bindingi245 – 268H-T-H motifPROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • regulation of cellular organohalogen metabolic process Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionActivator, DNA-binding
Biological processStress response, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G6175-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RCS-specific HTH-type transcriptional activator RclR
Alternative name(s):
Reactive chlorine resistance protein R
Gene namesi
Name:rclR
Synonyms:ykgD
Ordered Locus Names:b0305, JW0298
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13581 rclR

Pathology & Biotechi

Disruption phenotypei

Mutants are more sensitive to HOCl treatment than wild-type cells.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21C → A: Does not induce expression of target genes in response to HOCl. 1 Publication1
Mutagenesisi42H → A: Increases expression of the target genes in the absence of HOCl. 1 Publication1
Mutagenesisi75H → A: Increases expression of the target genes in the absence of HOCl. In vitro, increases DNA binding under reducing conditions. 1 Publication1
Mutagenesisi89C → A: Does not induce expression of target genes in response to HOCl. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001946131 – 284RCS-specific HTH-type transcriptional activator RclRAdd BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi21 ↔ 891 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP77379
PRIDEiP77379

Expressioni

Inductioni

Autoregulated. Induced in response to HOCl.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262810, 9 interactors
IntActiP77379, 5 interactors
STRINGi316385.ECDH10B_0293

Structurei

3D structure databases

ProteinModelPortaliP77379
SMRiP77379
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini177 – 278HTH araC/xylS-typePROSITE-ProRule annotationAdd BLAST102

Phylogenomic databases

eggNOGiENOG4108K01 Bacteria
COG2207 LUCA
HOGENOMiHOG000136816
InParanoidiP77379
KOiK21746
OMAiCMDFELG
PhylomeDBiP77379

Family and domain databases

InterProiView protein in InterPro
IPR032783 AraC_lig
IPR009057 Homeobox-like_sf
IPR018060 HTH_AraC
IPR011051 RmlC_Cupin_sf
IPR020449 Tscrpt_reg_HTH_AraC-type
PfamiView protein in Pfam
PF12852 Cupin_6, 1 hit
PF12833 HTH_18, 1 hit
PRINTSiPR00032 HTHARAC
SMARTiView protein in SMART
SM00342 HTH_ARAC, 1 hit
SUPFAMiSSF46689 SSF46689, 2 hits
SSF51182 SSF51182, 1 hit
PROSITEiView protein in PROSITE
PS01124 HTH_ARAC_FAMILY_2, 1 hit

Sequencei

Sequence statusi: Complete.

P77379-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALSRLLML NAPQGTIDKN CVLGSDWQLP HGAGELSVIR WHALTQGAAK
60 70 80 90 100
LEMPTGEIFT LRPGNVVLLP QNSAHRLSHV DNESTCIVCG TLRLQHSARY
110 120 130 140 150
FLTSLPETLF LAPVNHSVEY NWLREAIPFL QQESRSAMPG VDALCSQICA
160 170 180 190 200
TFFTLAVREW IAQVNTEKNI LSLLLHPRLG AVIQQMLEMP GHAWTVESLA
210 220 230 240 250
SIAHMSRASF AQLFRDVSGT TPLAVLTKLR LQIAAQMFSR ETLPVVVIAE
260 270 280
SVGYASESSF HKAFVREFGC TPGEYRERVR QLAP
Length:284
Mass (Da):31,474
Last modified:February 1, 1997 - v1
Checksum:i1896705347276040
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA Translation: AAB18032.1
U00096 Genomic DNA Translation: AAC73408.1
AP009048 Genomic DNA Translation: BAE76089.1
PIRiA64757
RefSeqiNP_414839.1, NC_000913.3
WP_000339594.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73408; AAC73408; b0305
BAE76089; BAE76089; BAE76089
GeneIDi945616
KEGGiecj:JW0298
eco:b0305
PATRICifig|511145.12.peg.312

Similar proteinsi

Entry informationi

Entry nameiRCLR_ECOLI
AccessioniPrimary (citable) accession number: P77379
Secondary accession number(s): Q2MCB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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