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Protein

Putative dimethyl sulfoxide reductase chain YnfE

Gene

ynfE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi60 – 601Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi64 – 641Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi96 – 961Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi196 – 1961MolybdenumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEcoCyc:G6845-MONOMER.
ECOL316407:JW1579-MONOMER.
MetaCyc:G6845-MONOMER.

Protein family/group databases

TCDBi5.A.3.3.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative dimethyl sulfoxide reductase chain YnfE (EC:1.8.99.-)
Short name:
DMSO reductase
Gene namesi
Name:ynfE
Ordered Locus Names:b1587, JW1579
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13843. ynfE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4343Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini44 – 808765Putative dimethyl sulfoxide reductase chain YnfEPRO_0000019147Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PaxDbiP77374.
PRIDEiP77374.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dmsDP698533EBI-556186,EBI-4406374

Protein-protein interaction databases

BioGridi4261571. 9 interactions.
DIPiDIP-12765N.
IntActiP77374. 5 interactions.
STRINGi511145.b1587.

Structurei

3D structure databases

ProteinModelPortaliP77374.
SMRiP77374. Positions 49-791.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 110624Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QY8. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000284390.
InParanoidiP77374.
KOiK07309.
OMAiITIERMP.
PhylomeDBiP77374.

Family and domain databases

InterProiIPR011888. Anaer_DMSO_reductase.
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR02166. dmsA_ynfE. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77374-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKNERMVGI SRRTLVKSTA IGSLALAAGG FSLPFTLRNA AAAVQQAREK
60 70 80 90 100
VVWGACSVNC GSRCALRLHV KDNEVTWVET DNTGSDEYGN HQVRACLRGR
110 120 130 140 150
SIRRRINHPD RLNYPMKRVG KRGEGKFERI SWDEALDTIA SSLKKTVEQY
160 170 180 190 200
GNEAVYIQYS SGIVGGNMTR SSPSASAVKR LMNCYGGSLN QYGSYSTAQI
210 220 230 240 250
SCAMPYTYGS NDGNSTTDIE NSKLVVMFGN NPAETRMSGG GITYLLEKAR
260 270 280 290 300
EKSNAKMIVI DPRYTDTAAG REDEWLPIRP GTDAALVAGI AWVLINENLV
310 320 330 340 350
DQPFLDKYCV GYDEKTLPAD APKNGHYKAY ILGEGDDKTA KTPQWASQIT
360 370 380 390 400
GIPEDRIIKL AREIGTAKPA YICQGWGPQR QANGELTARA IAMLPILTGN
410 420 430 440 450
VGISGGNSGA RESTYTITIE RLPVLDNPVK TSISCFSWTD AIDHGPQMTA
460 470 480 490 500
IRDGVRGKDK LDVPIKFIWN YAGNTLVNQH SDINKTHEIL QDESKCEMIV
510 520 530 540 550
VIENFMTSSA KYADILLPDL MTVEQEDIIP NDYAGNMGYL IFLQPVTSEK
560 570 580 590 600
FERKPIYWIL SEVAKRLGPD VYQKFTEGRT QEQWLQHLYA KMLAKDPALP
610 620 630 640 650
SYDELKKMGI YKRKDPNGHF VAYKAFRDDP EANPLKTPSG KIEIYSSRLA
660 670 680 690 700
EIARTWELEK DEVISPLPVY ASTFEGWNSP ERRTFPLQLF GFHYKSRTHS
710 720 730 740 750
TYGNIDLLKA ACRQEVWINP IDAQKRGIAN GDMVRVFNHR GEVRLPAKVT
760 770 780 790 800
PRILPGVSAM GQGAWHEANM SGDKIDHGGC VNTLTTLRPS PLAKGNPQHT

NLVEIEKI
Length:808
Mass (Da):89,780
Last modified:February 1, 1997 - v1
Checksum:iE7A6A626C9E46449
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74659.1.
AP009048 Genomic DNA. Translation: BAA15311.1.
PIRiE64914.
RefSeqiNP_416104.1. NC_000913.3.
WP_000041675.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74659; AAC74659; b1587.
BAA15311; BAA15311; BAA15311.
GeneIDi946135.
KEGGiecj:JW1579.
eco:b1587.
PATRICi32118476. VBIEscCol129921_1658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74659.1.
AP009048 Genomic DNA. Translation: BAA15311.1.
PIRiE64914.
RefSeqiNP_416104.1. NC_000913.3.
WP_000041675.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77374.
SMRiP77374. Positions 49-791.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261571. 9 interactions.
DIPiDIP-12765N.
IntActiP77374. 5 interactions.
STRINGi511145.b1587.

Protein family/group databases

TCDBi5.A.3.3.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Proteomic databases

PaxDbiP77374.
PRIDEiP77374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74659; AAC74659; b1587.
BAA15311; BAA15311; BAA15311.
GeneIDi946135.
KEGGiecj:JW1579.
eco:b1587.
PATRICi32118476. VBIEscCol129921_1658.

Organism-specific databases

EchoBASEiEB3604.
EcoGeneiEG13843. ynfE.

Phylogenomic databases

eggNOGiENOG4107QY8. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000284390.
InParanoidiP77374.
KOiK07309.
OMAiITIERMP.
PhylomeDBiP77374.

Enzyme and pathway databases

BioCyciEcoCyc:G6845-MONOMER.
ECOL316407:JW1579-MONOMER.
MetaCyc:G6845-MONOMER.

Miscellaneous databases

PROiP77374.

Family and domain databases

InterProiIPR011888. Anaer_DMSO_reductase.
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR02166. dmsA_ynfE. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYNFE_ECOLI
AccessioniPrimary (citable) accession number: P77374
Secondary accession number(s): P77291
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.