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Protein

Lipoprotein YafY

Gene

yafY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

When overproduced strongly induces degP through the activation of the two-component envelope stress response system CpxA/CpxR (PubMed:15252048).1 Publication

Enzyme and pathway databases

BioCyciEcoCyc:G6126-MONOMER.
ECOL316407:JW0240-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein YafY
Gene namesi
Name:yafY
Ordered Locus Names:b0251, JW0240
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13337. yafY.

Subcellular locationi

  • Cell inner membrane PROSITE-ProRule annotation1 Publication; Lipid-anchor PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi21 – 211C → A: Very poorly expressed. 1 Publication
Mutagenesisi22 – 232DD → SS: Still induces degP when overexpressed, remains in inner membrane. 1 Publication
Mutagenesisi60 – 601D → G: 5-fold decrease in degP induction when overexpressed. 1 Publication
Mutagenesisi61 – 611Y → F: 3-fold decrease in degP induction when overexpressed. 1 Publication
Mutagenesisi136 – 1361Q → R: 2-fold decrease in degP induction when overexpressed. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020PROSITE-ProRule annotationAdd
BLAST
Chaini21 – 147127Lipoprotein YafYPRO_0000050270Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi21 – 211N-palmitoyl cysteinePROSITE-ProRule annotation1 Publication
Lipidationi21 – 211S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP77365.

Interactioni

Protein-protein interaction databases

IntActiP77365. 3 interactions.
STRINGi511145.b0251.

Structurei

3D structure databases

ProteinModelPortaliP77365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To E.coli YfjS.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2378. LUCA.
HOGENOMiHOG000028675.

Family and domain databases

PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77365-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKTLPLLA LVATTLFLIA CDDRSDDLKA ISKFKDLTPP RFSDVVSHQD
60 70 80 90 100
DVSEEWSQVD YLSGPTLQVL RTRQSPDGCE DGSYYYLVDM QEKTVQPLMN
110 120 130 140
ALCIADNIKL EYQEVTDPYT KEKYFEYAHD GKLMGQLLIP SNPDNQE
Length:147
Mass (Da):16,815
Last modified:June 26, 2007 - v2
Checksum:iDB68B4C16A7ECD94
GO

Sequence cautioni

The sequence AAB08670 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA77920 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08670.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73354.2.
AP009048 Genomic DNA. Translation: BAA77920.1. Different initiation.
PIRiC64750.
RefSeqiNP_414785.4. NC_000913.3.
WP_000824223.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73354; AAC73354; b0251.
BAA77920; BAA77920; BAA77920.
GeneIDi944934.
KEGGiecj:JW0240.
eco:b0251.
PATRICi32115619. VBIEscCol129921_0253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08670.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73354.2.
AP009048 Genomic DNA. Translation: BAA77920.1. Different initiation.
PIRiC64750.
RefSeqiNP_414785.4. NC_000913.3.
WP_000824223.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP77365. 3 interactions.
STRINGi511145.b0251.

Proteomic databases

PaxDbiP77365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73354; AAC73354; b0251.
BAA77920; BAA77920; BAA77920.
GeneIDi944934.
KEGGiecj:JW0240.
eco:b0251.
PATRICi32115619. VBIEscCol129921_0253.

Organism-specific databases

EchoBASEiEB3121.
EcoGeneiEG13337. yafY.

Phylogenomic databases

eggNOGiCOG2378. LUCA.
HOGENOMiHOG000028675.

Enzyme and pathway databases

BioCyciEcoCyc:G6126-MONOMER.
ECOL316407:JW0240-MONOMER.

Miscellaneous databases

PROiP77365.

Family and domain databases

PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYAFY_ECOLI
AccessioniPrimary (citable) accession number: P77365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 26, 2007
Last modified: September 7, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.