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Protein

Glycerate kinase

Gene

glxK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-glycerate = ADP + 3-phospho-D-glycerate.

Cofactori

Mg2+1 Publication, Co2+1 Publication, Mn2+1 Publication, Fe2+1 Publication, Ca2+1 PublicationNote: Divalent metal cations; Mg2+ and Co2+ are better than Mn2+, Fe2+ or Ca2+.1 Publication

Enzyme regulationi

Inhibited by EDTA, p-hydroxy-mercuribenzoate and iodoacetate but not by NaF.1 Publication

Kineticsi

  1. KM=0.07 mM for glycerate1 Publication

    pH dependencei

    Optimum pH is 7.0-7.6.1 Publication

    Pathwayi: glycolate degradation

    This protein is involved in step 4 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate.
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Glyoxylate carboligase (gcl)
    3. 2-hydroxy-3-oxopropionate reductase (glxR)
    4. Glycerate kinase (glxK)
    This subpathway is part of the pathway glycolate degradation, which is itself part of Organic acid metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • glycerate 2-kinase activity Source: EcoCyc
    • glycerate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    • glycolate catabolic process Source: EcoCyc
    • glyoxylate catabolic process Source: EcoCyc
    • organic acid phosphorylation Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:GLY3KIN-MONOMER.
    ECOL316407:JW0502-MONOMER.
    MetaCyc:GLY3KIN-MONOMER.
    BRENDAi2.7.1.165. 2165.
    UniPathwayiUPA00864; UER00833.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerate kinase (EC:2.7.1.31)
    Alternative name(s):
    D-Glycerate-3-kinase
    GK2
    Gene namesi
    Name:glxK
    Synonyms:glxB5, ybbZ
    Ordered Locus Names:b0514, JW0502
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13621. glxK.

    Pathology & Biotechi

    Disruption phenotypei

    Loss of glycerate kinase activity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000715311 – 381Glycerate kinaseAdd BLAST381

    Proteomic databases

    PaxDbiP77364.
    PRIDEiP77364.

    Expressioni

    Inductioni

    In glycolate-grown cells but not in glucose-grown cells (at protein level).2 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi4262012. 9 interactors.
    IntActiP77364. 2 interactors.
    STRINGi511145.b0514.

    Structurei

    3D structure databases

    ProteinModelPortaliP77364.
    SMRiP77364.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycerate kinase type-1 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105BZW. Bacteria.
    COG1929. LUCA.
    HOGENOMiHOG000089298.
    InParanoidiP77364.
    KOiK00865.
    OMAiSIEMNEC.
    PhylomeDBiP77364.

    Family and domain databases

    Gene3Di3.90.1510.10. 1 hit.
    InterProiIPR018193. Glyc_kinase_flavodox-like-dom.
    IPR004381. Glycerate_kinase.
    [Graphical view]
    PANTHERiPTHR21599. PTHR21599. 1 hit.
    PfamiPF02595. Gly_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006078. GlxK. 1 hit.
    SUPFAMiSSF110738. SSF110738. 1 hit.
    TIGRFAMsiTIGR00045. TIGR00045. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P77364-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIVIAPDSF KESLSAEKCC QAIKAGFSTL FPDANYICLP IADGGEGTVD
    60 70 80 90 100
    AMVAATGGNI VTLEVCGPMG EKVNAFYGLT GDGKTAVIEM AAASGLMLVA
    110 120 130 140 150
    PEKRNPLLAS SFGTGELIRH ALDNDIRHII LGIGGSATVD GGMGMAQALG
    160 170 180 190 200
    VRFLDADGQA LAANGGNLAR VASIEMDECD PRLANCHIEV ACDVDNPLVG
    210 220 230 240 250
    ARGAAAVFGP QKGATPEMVE ELEQGLQNYA RVLQQQTEIN VCQMAGGGAA
    260 270 280 290 300
    GGMGIAAAVF LNADIKPGIE IVLNAVNLAQ AVQGAALVIT GEGRIDSQTA
    310 320 330 340 350
    GGKAPLGVAS VAKQFNVPVI GIAGVLGDGV EVVHQYGIDA VFSILPRLAP
    360 370 380
    LAEVLASGET NLFNSARNIA CAIKIGQGIK N
    Length:381
    Mass (Da):38,734
    Last modified:February 1, 1997 - v1
    Checksum:i0F902DF27DE63578
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U89279 Genomic DNA. Translation: AAB93855.1.
    U82664 Genomic DNA. Translation: AAB40266.1.
    U00096 Genomic DNA. Translation: AAC73616.1.
    AP009048 Genomic DNA. Translation: BAE76292.1.
    PIRiA64783.
    RefSeqiNP_415047.1. NC_000913.3.
    WP_001333621.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73616; AAC73616; b0514.
    BAE76292; BAE76292; BAE76292.
    GeneIDi945129.
    KEGGiecj:JW0502.
    eco:b0514.
    PATRICi32116185. VBIEscCol129921_0534.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U89279 Genomic DNA. Translation: AAB93855.1.
    U82664 Genomic DNA. Translation: AAB40266.1.
    U00096 Genomic DNA. Translation: AAC73616.1.
    AP009048 Genomic DNA. Translation: BAE76292.1.
    PIRiA64783.
    RefSeqiNP_415047.1. NC_000913.3.
    WP_001333621.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP77364.
    SMRiP77364.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262012. 9 interactors.
    IntActiP77364. 2 interactors.
    STRINGi511145.b0514.

    Proteomic databases

    PaxDbiP77364.
    PRIDEiP77364.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73616; AAC73616; b0514.
    BAE76292; BAE76292; BAE76292.
    GeneIDi945129.
    KEGGiecj:JW0502.
    eco:b0514.
    PATRICi32116185. VBIEscCol129921_0534.

    Organism-specific databases

    EchoBASEiEB3386.
    EcoGeneiEG13621. glxK.

    Phylogenomic databases

    eggNOGiENOG4105BZW. Bacteria.
    COG1929. LUCA.
    HOGENOMiHOG000089298.
    InParanoidiP77364.
    KOiK00865.
    OMAiSIEMNEC.
    PhylomeDBiP77364.

    Enzyme and pathway databases

    UniPathwayiUPA00864; UER00833.
    BioCyciEcoCyc:GLY3KIN-MONOMER.
    ECOL316407:JW0502-MONOMER.
    MetaCyc:GLY3KIN-MONOMER.
    BRENDAi2.7.1.165. 2165.

    Miscellaneous databases

    PROiP77364.

    Family and domain databases

    Gene3Di3.90.1510.10. 1 hit.
    InterProiIPR018193. Glyc_kinase_flavodox-like-dom.
    IPR004381. Glycerate_kinase.
    [Graphical view]
    PANTHERiPTHR21599. PTHR21599. 1 hit.
    PfamiPF02595. Gly_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006078. GlxK. 1 hit.
    SUPFAMiSSF110738. SSF110738. 1 hit.
    TIGRFAMsiTIGR00045. TIGR00045. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLXK2_ECOLI
    AccessioniPrimary (citable) accession number: P77364
    Secondary accession number(s): Q2MBR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: November 2, 2016
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    GK1 has a half-life of 92 minutes while GK2 has a half-life of 11 minutes at 49 degrees Celsius.

    Caution

    E.coli has 2 glycerate kinases, GK1 and GK2; it is not clear which gene encodes which enzyme. PubMed:5325263 may be a mix of GK1 and GK2.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families
    4. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.