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Protein

Glycerate 3-kinase

Gene

glxK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

GK1 has a half-life of 92 minutes while GK2 has a half-life of 11 minutes at 49 degrees Celsius.

Caution

E.coli has 2 glycerate kinases, GK1 and GK2; it is not clear which gene encodes which enzyme. PubMed:5325263 may be a mix of GK1 and GK2.Curated

Catalytic activityi

ATP + D-glycerate = ADP + 3-phospho-D-glycerate.

Cofactori

Mg2+1 Publication, Co2+1 Publication, Mn2+1 Publication, Fe2+1 Publication, Ca2+1 PublicationNote: Divalent metal cations; Mg2+ and Co2+ are better than Mn2+, Fe2+ or Ca2+.1 Publication

Enzyme regulationi

Inhibited by EDTA, p-hydroxy-mercuribenzoate and iodoacetate but not by NaF.1 Publication

Kineticsi

  1. KM=0.07 mM for glycerate1 Publication

    pH dependencei

    Optimum pH is 7.0-7.6.1 Publication

    Pathwayi: glycolate degradation

    This protein is involved in step 4 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate.
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Glyoxylate carboligase (gcl)
    3. 2-hydroxy-3-oxopropionate reductase (glxR)
    4. Glycerate 3-kinase (glxK)
    This subpathway is part of the pathway glycolate degradation, which is itself part of Organic acid metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • glycerate 2-kinase activity Source: EcoCyc
    • glycerate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    • glycolate catabolic process Source: EcoCyc
    • glyoxylate catabolic process Source: EcoCyc
    • organic acid phosphorylation Source: InterPro

    Keywordsi

    Molecular functionKinase, Transferase
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:GLY3KIN-MONOMER
    MetaCyc:GLY3KIN-MONOMER
    BRENDAi2.7.1.165 2165
    UniPathwayiUPA00864; UER00833

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerate 3-kinase (EC:2.7.1.31)
    Alternative name(s):
    D-Glycerate-3-kinase
    Glycerate kinase 2
    Short name:
    GK2
    Gene namesi
    Name:glxK
    Synonyms:glxB5, ybbZ
    Ordered Locus Names:b0514, JW0502
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13621 glxK

    Pathology & Biotechi

    Disruption phenotypei

    Loss of glycerate kinase activity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000715311 – 381Glycerate 3-kinaseAdd BLAST381

    Proteomic databases

    PaxDbiP77364
    PRIDEiP77364

    Expressioni

    Inductioni

    In glycolate-grown cells but not in glucose-grown cells (at protein level).2 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi4262012, 12 interactors
    IntActiP77364, 2 interactors
    STRINGi316385.ECDH10B_0470

    Structurei

    3D structure databases

    ProteinModelPortaliP77364
    SMRiP77364
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycerate kinase type-1 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105BZW Bacteria
    COG1929 LUCA
    HOGENOMiHOG000089298
    InParanoidiP77364
    KOiK00865
    OMAiSIEMNEC
    PhylomeDBiP77364

    Family and domain databases

    Gene3Di3.40.50.10350, 2 hits
    3.90.1510.10, 1 hit
    InterProiView protein in InterPro
    IPR018193 Glyc_kinase_flavodox-like_fold
    IPR004381 Glycerate_kinase
    IPR018197 Glycerate_kinase_RE-like
    IPR036129 Glycerate_kinase_sf
    PANTHERiPTHR21599 PTHR21599, 1 hit
    PfamiView protein in Pfam
    PF02595 Gly_kinase, 1 hit
    PIRSFiPIRSF006078 GlxK, 1 hit
    SUPFAMiSSF110738 SSF110738, 1 hit
    TIGRFAMsiTIGR00045 TIGR00045, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P77364-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIVIAPDSF KESLSAEKCC QAIKAGFSTL FPDANYICLP IADGGEGTVD
    60 70 80 90 100
    AMVAATGGNI VTLEVCGPMG EKVNAFYGLT GDGKTAVIEM AAASGLMLVA
    110 120 130 140 150
    PEKRNPLLAS SFGTGELIRH ALDNDIRHII LGIGGSATVD GGMGMAQALG
    160 170 180 190 200
    VRFLDADGQA LAANGGNLAR VASIEMDECD PRLANCHIEV ACDVDNPLVG
    210 220 230 240 250
    ARGAAAVFGP QKGATPEMVE ELEQGLQNYA RVLQQQTEIN VCQMAGGGAA
    260 270 280 290 300
    GGMGIAAAVF LNADIKPGIE IVLNAVNLAQ AVQGAALVIT GEGRIDSQTA
    310 320 330 340 350
    GGKAPLGVAS VAKQFNVPVI GIAGVLGDGV EVVHQYGIDA VFSILPRLAP
    360 370 380
    LAEVLASGET NLFNSARNIA CAIKIGQGIK N
    Length:381
    Mass (Da):38,734
    Last modified:February 1, 1997 - v1
    Checksum:i0F902DF27DE63578
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U89279 Genomic DNA Translation: AAB93855.1
    U82664 Genomic DNA Translation: AAB40266.1
    U00096 Genomic DNA Translation: AAC73616.1
    AP009048 Genomic DNA Translation: BAE76292.1
    PIRiA64783
    RefSeqiNP_415047.1, NC_000913.3
    WP_001333621.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73616; AAC73616; b0514
    BAE76292; BAE76292; BAE76292
    GeneIDi945129
    KEGGiecj:JW0502
    eco:b0514
    PATRICifig|1411691.4.peg.1764

    Similar proteinsi

    Entry informationi

    Entry nameiGLXK2_ECOLI
    AccessioniPrimary (citable) accession number: P77364
    Secondary accession number(s): Q2MBR4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: March 28, 2018
    This is version 127 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health