P77354 (YAFU_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative inner membrane protein yafU

Gene names

Name:	yafU
Ordered Locus Names:	b0218, JW0207

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Subcellular location	Cell inner membrane; Multi-pass membrane protein. Note: When overexpressed.
Caution	Could be the product of a pseudogene.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 112	112	Putative inner membrane protein yafU		PRO_0000168543
Regions
	Topological domain	1 – 21	21	Cytoplasmic Potential
	Transmembrane	22 – 42	21	Helical; Potential
	Topological domain	43	1	Periplasmic Potential
	Transmembrane	44 – 64	21	Helical; Potential
	Topological domain	65 – 112	48	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P77354 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7B8E901E02429F0D	FASTA	112	12,136
	10 20 30 40 50 60 MSSERDLVNF LGDFSMDVAK AVIAGGVATA IGSLASFACV SFGFPVILVG GAILLTGIVC 70 80 90 100 110 TVVLNEIDAQ CHLSEKLKYA IRDGLKRQQE LDKWKRENMT PFMYVLNTPP VI

References

[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U70214 Genomic DNA. Translation: AAB08640.1. U00096 Genomic DNA. No translation available. AP009048 Genomic DNA. Translation: BAE76046.1.
PIR	C64746.
3D structure databases
ProteinModelPortal	P77354.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000017111; EBESCP00000016402; EBESCG00000016170.
GenomeReviews	Gene locus JW0207 in contig AP009048_GR. Gene locus b0218 in contig U00096_GR.
KEGG	ecj:JW0207.
Organism-specific databases
EchoBASE	EB3117.
EcoGene	EG13333. yafU.
Phylogenomic databases
GeneTree	EBGT00050000009736.
HOGENOM	HBG086531.
OMA	MRTIEFI.
Enzyme and pathway databases
BioCyc	EcoCyc:G6102-MONOMER.
Gene expression databases
Genevestigator	P77354.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

YAFU_ECOLI

Accession

Primary (citable) accession number: P77354
Secondary accession number(s): Q2MCG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program