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Protein

Periplasmic murein peptide-binding protein

Gene

mppA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the uptake of the murein peptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate. Also transports some alpha-linked peptides such as Pro-Phe-Lys with low affinity. The transport is effected by the oligopeptide permease system.

GO - Molecular functioni

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • transmembrane transport Source: InterPro
  • tripeptide transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Peptide transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6665-MONOMER.
ECOL316407:JW1322-MONOMER.
MetaCyc:G6665-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.1. the atp-binding cassette (abc) superfamily.
3.A.1.5.41. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic murein peptide-binding protein
Gene namesi
Name:mppA
Synonyms:ynaH
Ordered Locus Names:b1329, JW1322
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13376. mppA.

Subcellular locationi

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: InterPro
  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Chaini23 – 537515Periplasmic murein peptide-binding proteinPRO_0000031792Add
BLAST

Proteomic databases

EPDiP77348.
PaxDbiP77348.
PRIDEiP77348.

2D gel databases

SWISS-2DPAGEP77348.

Interactioni

Protein-protein interaction databases

BioGridi4260155. 258 interactions.
STRINGi511145.b1329.

Structurei

Secondary structure

1
537
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 438Combined sources
Turni50 – 523Combined sources
Helixi56 – 6510Combined sources
Beta strandi69 – 724Combined sources
Beta strandi78 – 8710Combined sources
Beta strandi89 – 9810Combined sources
Helixi112 – 12312Combined sources
Helixi125 – 1273Combined sources
Turni130 – 1323Combined sources
Helixi133 – 1375Combined sources
Helixi143 – 1475Combined sources
Helixi153 – 1553Combined sources
Beta strandi156 – 1627Combined sources
Beta strandi165 – 1728Combined sources
Helixi177 – 1804Combined sources
Helixi184 – 1863Combined sources
Helixi191 – 1944Combined sources
Helixi198 – 2014Combined sources
Turni203 – 2053Combined sources
Beta strandi210 – 2189Combined sources
Turni219 – 2213Combined sources
Beta strandi222 – 2276Combined sources
Helixi234 – 2363Combined sources
Beta strandi240 – 2467Combined sources
Helixi252 – 2587Combined sources
Helixi270 – 27910Combined sources
Helixi281 – 2833Combined sources
Beta strandi288 – 29710Combined sources
Beta strandi299 – 3024Combined sources
Helixi303 – 3053Combined sources
Helixi307 – 3159Combined sources
Helixi319 – 3246Combined sources
Turni325 – 3273Combined sources
Beta strandi335 – 3384Combined sources
Turni351 – 3544Combined sources
Helixi357 – 37014Combined sources
Beta strandi374 – 3774Combined sources
Beta strandi381 – 3877Combined sources
Helixi389 – 40618Combined sources
Beta strandi410 – 4156Combined sources
Helixi417 – 42610Combined sources
Beta strandi430 – 4378Combined sources
Beta strandi439 – 4424Combined sources
Helixi444 – 4474Combined sources
Helixi448 – 4503Combined sources
Helixi464 – 47310Combined sources
Helixi479 – 49618Combined sources
Beta strandi498 – 5069Combined sources
Beta strandi509 – 5113Combined sources
Helixi529 – 5313Combined sources
Beta strandi533 – 5353Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3O9PX-ray2.07A23-537[»]
4TOZX-ray1.50A/B23-537[»]
ProteinModelPortaliP77348.
SMRiP77348. Positions 29-537.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77348.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QSI. Bacteria.
COG4166. LUCA.
HOGENOMiHOG000179191.
InParanoidiP77348.
KOiK15580.
OMAiIDRRVMA.
OrthoDBiEOG6V7BH5.
PhylomeDBiP77348.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77348-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHSVSVTCC ALLVSSISLS YAAEVPSGTV LAEKQELVRH IKDEPASLDP
60 70 80 90 100
AKAVGLPEIQ VIRDLFEGLV NQNEKGEIVP GVATQWKSND NRIWTFTLRD
110 120 130 140 150
NAKWADGTPV TAQDFVYSWQ RLVDPKTLSP FAWFAALAGI NNAQAIIDGK
160 170 180 190 200
ATPDQLGVTA VDAHTLKIQL DKPLPWFVNL TANFAFFPVQ KANVESGKEW
210 220 230 240 250
TKPGNLIGNG AYVLKERVVN EKLVVVPNTH YWDNAKTVLQ KVTFLPINQE
260 270 280 290 300
SAATKRYLAG DIDITESFPK NMYQKLLKDI PGQVYTPPQL GTYYYAFNTQ
310 320 330 340 350
KGPTADQRVR LALSMTIDRR LMTEKVLGTG EKPAWHFTPD VTAGFTPEPS
360 370 380 390 400
PFEQMSQEEL NAQAKTLLSA AGYGPQKPLK LTLLYNTSEN HQKIAIAVAS
410 420 430 440 450
MWKKNLGVDV KLQNQEWKTY IDSRNTGNFD VIRASWVGDY NEPSTFLTLL
460 470 480 490 500
TSTHSGNISR FNNPAYDKVL AQASTENTVK ARNADYNAAE KILMEQAPIA
510 520 530
PIYQYTNGRL IKPWLKGYPI NNPEDVAYSR TMYIVKH
Length:537
Mass (Da):59,900
Last modified:December 15, 1998 - v2
Checksum:iC6A17656836DC3AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88242 Genomic DNA. Translation: AAC38216.1.
U00096 Genomic DNA. Translation: AAC74411.2.
AP009048 Genomic DNA. Translation: BAA14922.2.
PIRiD64882.
RefSeqiNP_415845.2. NC_000913.3.
WP_000683020.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74411; AAC74411; b1329.
BAA14922; BAA14922; BAA14922.
GeneIDi945951.
KEGGiecj:JW1322.
eco:b1329.
PATRICi32117934. VBIEscCol129921_1387.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88242 Genomic DNA. Translation: AAC38216.1.
U00096 Genomic DNA. Translation: AAC74411.2.
AP009048 Genomic DNA. Translation: BAA14922.2.
PIRiD64882.
RefSeqiNP_415845.2. NC_000913.3.
WP_000683020.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3O9PX-ray2.07A23-537[»]
4TOZX-ray1.50A/B23-537[»]
ProteinModelPortaliP77348.
SMRiP77348. Positions 29-537.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260155. 258 interactions.
STRINGi511145.b1329.

Protein family/group databases

TCDBi3.A.1.5.1. the atp-binding cassette (abc) superfamily.
3.A.1.5.41. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP77348.

Proteomic databases

EPDiP77348.
PaxDbiP77348.
PRIDEiP77348.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74411; AAC74411; b1329.
BAA14922; BAA14922; BAA14922.
GeneIDi945951.
KEGGiecj:JW1322.
eco:b1329.
PATRICi32117934. VBIEscCol129921_1387.

Organism-specific databases

EchoBASEiEB3158.
EcoGeneiEG13376. mppA.

Phylogenomic databases

eggNOGiENOG4107QSI. Bacteria.
COG4166. LUCA.
HOGENOMiHOG000179191.
InParanoidiP77348.
KOiK15580.
OMAiIDRRVMA.
OrthoDBiEOG6V7BH5.
PhylomeDBiP77348.

Enzyme and pathway databases

BioCyciEcoCyc:G6665-MONOMER.
ECOL316407:JW1322-MONOMER.
MetaCyc:G6665-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP77348.
PROiP77348.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MppA, a periplasmic binding protein essential for import of the bacterial cell wall peptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate."
    Park J.T., Raychaudhuri D., Li H., Normark S., Mengin-Lecreulx D.
    J. Bacteriol. 180:1215-1223(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-32.
    Strain: K12 / AT980.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiMPPA_ECOLI
AccessioniPrimary (citable) accession number: P77348
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: March 16, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.