P77339 (YAFT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized lipoprotein YafT

Gene names

Name:	yafT
Ordered Locus Names:	b0217, JW0206

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Subcellular location

Cell membrane; Lipid-anchor Probable.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 261	243	Uncharacterized lipoprotein YafT		PRO_0000013785
Amino acid modifications
	Lipidation	19	1	N-palmitoyl cysteine Potential
	Lipidation	19	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	P77339 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 3EA61CC9E636CF7F	FASTA	261	29,606
	10 20 30 40 50 60 MNSKKLCCIC VLFSLLAGCA SESSIDEKKK KAQVTQSNIN KNTPQQLTDK DLFGNETTLA 70 80 90 100 110 120 VSEEDIQAAL DGDEFRVPLN SPVILVQSGN RAPETIMQEE MRKYYTVSTF SGIPDRQKPL 130 140 150 160 170 180 TCNKNKDKNE NEDVASAENM NWMQALRFVA AKGHQKAIIV YQDMLQTGKY DSALKSTVWS 190 200 210 220 230 240 DYKNDKLTDA ISLRYLVRFT LVDVATGEWA TWSPVNYEYK VLPPLPDKNE ASTTDMTEQQ 250 260 IMQLKQKTYK AMVKDLVNRY Q

References

[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U70214 Genomic DNA. Translation: AAB08638.1. U00096 Genomic DNA. Translation: AAC73321.1. AP009048 Genomic DNA. Translation: BAA77887.1.
PIR	B64746.
RefSeq	NP_414752.1. NC_000913.2. YP_488513.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P77339.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77339. 1 interaction.
STRING	511145.b0217.
Proteomic databases
PaxDb	P77339.
PRIDE	P77339.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73321; AAC73321; b0217. BAA77887; BAA77887; BAA77887.
GeneID	12930764. 949009.
KEGG	ecj:Y75_p0207. eco:b0217.
PATRIC	32115547. VBIEscCol129921_0218.
Organism-specific databases
EchoBASE	EB3116.
EcoGene	EG13332. yafT.
Phylogenomic databases
eggNOG	NOG86611.
HOGENOM	HOG000116945.
OMA	YLCTTIF.
ProtClustDB	CLSK879578.
Enzyme and pathway databases
BioCyc	EcoCyc:G6101-MONOMER. ECOL316407:JW0206-MONOMER.
Gene expression databases
Genevestigator	P77339.
Family and domain databases
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAFT_ECOLI

Accession

Primary (citable) accession number: P77339

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program