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Protein

Mechanosensitive channel MscK

Gene

mscK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mechanosensitive channel that opens in response to membrane tension and specific ionic conditions. Requires high concentrations of external K+, NH4+, Rb+ or Cs+ to gate. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Forms an ion channel of 1.0 nanosiemens conductance. The channel can remain active for between 30 seconds and over 3 minutes; it does not desensitize upon extended pressure. Its activity is masked in wild-type cells by the MscS channel.3 Publications

GO - Biological processi

  • cellular water homeostasis Source: EcoCyc
  • potassium ion transport Source: UniProtKB-KW
  • transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciEcoCyc:G6255-MONOMER.
ECOL316407:JW0454-MONOMER.

Protein family/group databases

TCDBi1.A.23.1.1. the small conductance mechanosensitive ion channel (mscs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mechanosensitive channel MscK
Alternative name(s):
Potassium efflux system KefA
Gene namesi
Name:mscK
Synonyms:aefA, kefA
Ordered Locus Names:b0465, JW0454
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14240. mscK.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 499466PeriplasmicSequence analysisAdd
BLAST
Transmembranei500 – 52021HelicalSequence analysisAdd
BLAST
Topological domaini521 – 56040CytoplasmicSequence analysisAdd
BLAST
Transmembranei561 – 58121HelicalSequence analysisAdd
BLAST
Topological domaini582 – 5821PeriplasmicSequence analysis
Transmembranei583 – 60321HelicalSequence analysisAdd
BLAST
Topological domaini604 – 63431CytoplasmicSequence analysisAdd
BLAST
Transmembranei635 – 65521HelicalSequence analysisAdd
BLAST
Topological domaini656 – 6572PeriplasmicSequence analysis
Transmembranei658 – 67821HelicalSequence analysisAdd
BLAST
Topological domaini679 – 69214CytoplasmicSequence analysisAdd
BLAST
Transmembranei693 – 71321HelicalSequence analysisAdd
BLAST
Topological domaini714 – 72815PeriplasmicSequence analysisAdd
BLAST
Transmembranei729 – 74921HelicalSequence analysisAdd
BLAST
Topological domaini750 – 79647CytoplasmicSequence analysisAdd
BLAST
Transmembranei797 – 81721HelicalSequence analysisAdd
BLAST
Topological domaini818 – 83922PeriplasmicSequence analysisAdd
BLAST
Transmembranei840 – 86021HelicalSequence analysisAdd
BLAST
Topological domaini861 – 88626CytoplasmicSequence analysisAdd
BLAST
Transmembranei887 – 90721HelicalSequence analysisAdd
BLAST
Topological domaini908 – 92114PeriplasmicSequence analysisAdd
BLAST
Transmembranei922 – 94221HelicalSequence analysisAdd
BLAST
Topological domaini943 – 1120178CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi922 – 9221G → S: Prevents growth in medium containing high levels of potassium in the presence of betaine. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence analysisAdd
BLAST
Chaini34 – 11201087Mechanosensitive channel MscKPRO_0000043366Add
BLAST

Proteomic databases

PaxDbiP77338.
PRIDEiP77338.

Interactioni

Protein-protein interaction databases

BioGridi4259842. 245 interactions.
DIPiDIP-10070N.
IntActiP77338. 3 interactions.
STRINGi511145.b0465.

Structurei

3D structure databases

ProteinModelPortaliP77338.
SMRiP77338. Positions 844-1098.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili43 – 9856Sequence analysisAdd
BLAST
Coiled coili126 – 266141Sequence analysisAdd
BLAST
Coiled coili360 – 42263Sequence analysisAdd
BLAST
Coiled coili1057 – 108125Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EGS. Bacteria.
COG3264. LUCA.
HOGENOMiHOG000280842.
InParanoidiP77338.
KOiK05802.
OMAiLHTPKAI.
OrthoDBiEOG6J1DC5.
PhylomeDBiP77338.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR006686. MscS_channel_CS.
IPR011014. MscS_channel_TM-2.
IPR025692. MscS_IM_dom1.
IPR024393. MscS_porin.
[Graphical view]
PfamiPF00924. MS_channel. 1 hit.
PF12795. MscS_porin. 1 hit.
PF12794. MscS_TM. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.
SSF82861. SSF82861. 1 hit.
PROSITEiPS01246. UPF0003. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMFQYYKRS RHFVFSAFIA FVFVLLCQNT AFARASSNGD LPTKADLQAQ
60 70 80 90 100
LDSLNKQKDL SAQDKLVQQD LTDTLATLDK IDRIKEETVQ LRQKVAEAPE
110 120 130 140 150
KMRQATAALT ALSDVDNDEE TRKILSTLSL RQLETRVAQA LDDLQNAQND
160 170 180 190 200
LASYNSQLVS LQTQPERVQN AMYNASQQLQ QIRSRLDGTD VGETALRPSQ
210 220 230 240 250
KVLMQAQQAL LNAEIDQQRK SLEGNTVLQD TLQKQRDYVT ANSARLEHQL
260 270 280 290 300
QLLQEAVNSK RLTLTEKTAQ EAVSPDEAAR IQANPLVKQE LEINQQLSQR
310 320 330 340 350
LITATENGNQ LMQQNIKVKN WLERALQSER NIKEQIAVLK GSLLLSRILY
360 370 380 390 400
QQQQTLPSAD ELENMTNRIA DLRLEQFEVN QQRDALFQSD AFVNKLEEGH
410 420 430 440 450
TNEVNSEVHD ALLQVVDMRR ELLDQLNKQL GNQLMMAINL QINQQQLMSV
460 470 480 490 500
SKNLKSILTQ QIFWVNSNRP MDWDWIKAFP QSLKDEFKSM KITVNWQKAW
510 520 530 540 550
PAVFIAFLAG LPLLLIAGLI HWRLGWLKAY QQKLASAVGS LRNDSQLNTP
560 570 580 590 600
KAILIDLIRA LPVCLIILAV GLILLTMQLN ISELLWSFSK KLAIFWLVFG
610 620 630 640 650
LCWKVLEKNG VAVRHFGMPE QQTSHWRRQI VRISLALLPI HFWSVVAELS
660 670 680 690 700
PLHLMDDVLG QAMIFFNLLL IAFLVWPMCR ESWRDKESHT MRLVTITVLS
710 720 730 740 750
IIPIALMVLT ATGYFYTTLR LAGRWIETVY LVIIWNLLYQ TVLRGLSVAA
760 770 780 790 800
RRIAWRRALA RRQNLVKEGA EGAEPPEEPT IALEQVNQQT LRITMLLMFA
810 820 830 840 850
LFGVMFWAIW SDLITVFSYL DSITLWHYNG TEAGAAVVKN VTMGSLLFAI
860 870 880 890 900
IASMVAWALI RNLPGLLEVL VLSRLNMRQG ASYAITTILN YIIIAVGAMT
910 920 930 940 950
VFGSLGVSWD KLQWLAAALS VGLGFGLQEI FGNFVSGLII LFERPVRIGD
960 970 980 990 1000
TVTIGSFSGT VSKIRIRATT ITDFDRKEVI IPNKAFVTER LINWSLTDTT
1010 1020 1030 1040 1050
TRLVIRLGVA YGSDLEKVRK VLLKAATEHP RVMHEPMPEV FFTAFGASTL
1060 1070 1080 1090 1100
DHELRLYVRE LRDRSRTVDE LNRTIDQLCR ENDINIAFNQ LEVHLHNEKG
1110 1120
DEVTEVKRDY KGDDPTPAVG
Length:1,120
Mass (Da):127,215
Last modified:February 1, 1997 - v1
Checksum:i809895660D2BD444
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07802 Genomic DNA. Translation: CAA69140.1.
U82664 Genomic DNA. Translation: AAB40219.1.
U00096 Genomic DNA. Translation: AAC73567.1.
AP009048 Genomic DNA. Translation: BAE76244.1.
PIRiH64776.
RefSeqiNP_414998.1. NC_000913.3.
WP_000177732.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73567; AAC73567; b0465.
BAE76244; BAE76244; BAE76244.
GeneIDi945132.
KEGGiecj:JW0454.
eco:b0465.
PATRICi32116083. VBIEscCol129921_0483.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07802 Genomic DNA. Translation: CAA69140.1.
U82664 Genomic DNA. Translation: AAB40219.1.
U00096 Genomic DNA. Translation: AAC73567.1.
AP009048 Genomic DNA. Translation: BAE76244.1.
PIRiH64776.
RefSeqiNP_414998.1. NC_000913.3.
WP_000177732.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77338.
SMRiP77338. Positions 844-1098.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259842. 245 interactions.
DIPiDIP-10070N.
IntActiP77338. 3 interactions.
STRINGi511145.b0465.

Protein family/group databases

TCDBi1.A.23.1.1. the small conductance mechanosensitive ion channel (mscs) family.

Proteomic databases

PaxDbiP77338.
PRIDEiP77338.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73567; AAC73567; b0465.
BAE76244; BAE76244; BAE76244.
GeneIDi945132.
KEGGiecj:JW0454.
eco:b0465.
PATRICi32116083. VBIEscCol129921_0483.

Organism-specific databases

EchoBASEiEB3991.
EcoGeneiEG14240. mscK.

Phylogenomic databases

eggNOGiENOG4105EGS. Bacteria.
COG3264. LUCA.
HOGENOMiHOG000280842.
InParanoidiP77338.
KOiK05802.
OMAiLHTPKAI.
OrthoDBiEOG6J1DC5.
PhylomeDBiP77338.

Enzyme and pathway databases

BioCyciEcoCyc:G6255-MONOMER.
ECOL316407:JW0454-MONOMER.

Miscellaneous databases

PROiP77338.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR006686. MscS_channel_CS.
IPR011014. MscS_channel_TM-2.
IPR025692. MscS_IM_dom1.
IPR024393. MscS_porin.
[Graphical view]
PfamiPF00924. MS_channel. 1 hit.
PF12795. MscS_porin. 1 hit.
PF12794. MscS_TM. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.
SSF82861. SSF82861. 1 hit.
PROSITEiPS01246. UPF0003. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterisation of the aefA locus of E.coli."
    Jones M.A., McLaggen D., Epstein W., Booth I.R.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity."
    Levina N., Toetemeyer S., Stokes N.R., Louis P., Jones M.A., Booth I.R.
    EMBO J. 18:1730-1737(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / MJF379.
  6. "Ionic regulation of MscK, a mechanosensitive channel from Escherichia coli."
    Li Y., Moe P.C., Chandrasekaran S., Booth I.R., Blount P.
    EMBO J. 21:5323-5330(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE NAME.
  7. "Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli."
    McLaggan D., Jones M.A., Gouesbet G., Levina N., Lindey S., Epstein W., Booth I.R.
    Mol. Microbiol. 43:521-536(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF GLY-922.
    Strain: K12.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMSCK_ECOLI
AccessioniPrimary (citable) accession number: P77338
Secondary accession number(s): Q2MBW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 17, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.