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Reviewed, UniProtKB/Swiss-Prot P77338 (KEFA_ECOLI)

Last modified November 24, 2009. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Potassium efflux system kefA
      Short name=Protein aefA
Gene names
Name: kefA
Synonyms: aefA, mscK
Ordered Locus Names: b0465, JW0454
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length1120 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Forms an ion channel of 1.0 nanosiemens conductance. The channel can remain active for between 30 seconds and over 3 minutes; it does not desensitize upon extended pressure. Its activity is masked in wild-type cells by the mscS channel.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the mscS (TC 1.A.23) family. [View classification]

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Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainCoiled coil
Signal
Transmembrane
   LigandPotassium
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processpotassium ion transport

Inferred from electronic annotation. Source: UniProtKB-KW

transmembrane transport

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionpotassium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 11201087Potassium efflux system kefA
PRO_0000043366

Regions

Transmembrane500 – 52021 Potential
Transmembrane561 – 58121 Potential
Transmembrane583 – 60321 Potential
Transmembrane635 – 65521 Potential
Transmembrane658 – 67821 Potential
Transmembrane693 – 71321 Potential
Transmembrane729 – 74921 Potential
Transmembrane797 – 81721 Potential
Transmembrane840 – 86021 Potential
Transmembrane887 – 90721 Potential
Transmembrane922 – 94221 Potential
Coiled coil43 – 9856 Potential
Coiled coil126 – 266141 Potential
Coiled coil360 – 42263 Potential
Coiled coil1057 – 108125 Potential

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Sequences

Sequence LengthMass (Da)Tools
P77338-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 809895660D2BD444

FASTA1,120127,215
        10         20         30         40         50         60 
MTMFQYYKRS RHFVFSAFIA FVFVLLCQNT AFARASSNGD LPTKADLQAQ LDSLNKQKDL 

        70         80         90        100        110        120 
SAQDKLVQQD LTDTLATLDK IDRIKEETVQ LRQKVAEAPE KMRQATAALT ALSDVDNDEE 

       130        140        150        160        170        180 
TRKILSTLSL RQLETRVAQA LDDLQNAQND LASYNSQLVS LQTQPERVQN AMYNASQQLQ 

       190        200        210        220        230        240 
QIRSRLDGTD VGETALRPSQ KVLMQAQQAL LNAEIDQQRK SLEGNTVLQD TLQKQRDYVT 

       250        260        270        280        290        300 
ANSARLEHQL QLLQEAVNSK RLTLTEKTAQ EAVSPDEAAR IQANPLVKQE LEINQQLSQR 

       310        320        330        340        350        360 
LITATENGNQ LMQQNIKVKN WLERALQSER NIKEQIAVLK GSLLLSRILY QQQQTLPSAD 

       370        380        390        400        410        420 
ELENMTNRIA DLRLEQFEVN QQRDALFQSD AFVNKLEEGH TNEVNSEVHD ALLQVVDMRR 

       430        440        450        460        470        480 
ELLDQLNKQL GNQLMMAINL QINQQQLMSV SKNLKSILTQ QIFWVNSNRP MDWDWIKAFP 

       490        500        510        520        530        540 
QSLKDEFKSM KITVNWQKAW PAVFIAFLAG LPLLLIAGLI HWRLGWLKAY QQKLASAVGS 

       550        560        570        580        590        600 
LRNDSQLNTP KAILIDLIRA LPVCLIILAV GLILLTMQLN ISELLWSFSK KLAIFWLVFG 

       610        620        630        640        650        660 
LCWKVLEKNG VAVRHFGMPE QQTSHWRRQI VRISLALLPI HFWSVVAELS PLHLMDDVLG 

       670        680        690        700        710        720 
QAMIFFNLLL IAFLVWPMCR ESWRDKESHT MRLVTITVLS IIPIALMVLT ATGYFYTTLR 

       730        740        750        760        770        780 
LAGRWIETVY LVIIWNLLYQ TVLRGLSVAA RRIAWRRALA RRQNLVKEGA EGAEPPEEPT 

       790        800        810        820        830        840 
IALEQVNQQT LRITMLLMFA LFGVMFWAIW SDLITVFSYL DSITLWHYNG TEAGAAVVKN 

       850        860        870        880        890        900 
VTMGSLLFAI IASMVAWALI RNLPGLLEVL VLSRLNMRQG ASYAITTILN YIIIAVGAMT 

       910        920        930        940        950        960 
VFGSLGVSWD KLQWLAAALS VGLGFGLQEI FGNFVSGLII LFERPVRIGD TVTIGSFSGT 

       970        980        990       1000       1010       1020 
VSKIRIRATT ITDFDRKEVI IPNKAFVTER LINWSLTDTT TRLVIRLGVA YGSDLEKVRK 

      1030       1040       1050       1060       1070       1080 
VLLKAATEHP RVMHEPMPEV FFTAFGASTL DHELRLYVRE LRDRSRTVDE LNRTIDQLCR 

      1090       1100       1110       1120 
ENDINIAFNQ LEVHLHNEKG DEVTEVKRDY KGDDPTPAVG

« Hide

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References

« Hide 'large scale' references
[1]"Characterisation of the aefA locus of E.coli."
Jones M.A., McLaggen D., Epstein W., Booth I.R.
Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity."
Levina N., Toetemeyer S., Stokes N.R., Louis P., Jones M.A., Booth I.R.
EMBO J. 18:1730-1737(1999) [PubMed: 10202137] [Abstract]
Cited for: CHARACTERIZATION.
Strain: K12 / MJF379.
[6]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: K12 / MG1655 / ATCC 47076.

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Cross-references

Sequence databases

Y07802 Genomic DNA. Translation: CAA69140.1.
U82664 Genomic DNA. Translation: AAB40219.1.
U00096 Genomic DNA. Translation: AAC73567.1.
AP009048 Genomic DNA. Translation: BAE76244.1.
PIRH64776.
RefSeqAP_001114.1.
NP_414998.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:10070N.
STRINGP77338.

Protein family/group databases

TCDB1.A.23.1.1. small conductance mechanosensitive ion channel (MscS) family.

Proteomic databases

PRIDEP77338.

Genome annotation databases

GeneID945132.
GenomeReviewsGene locus JW0454 in contig AP009048_GR.
Gene locus b0465 in contig U00096_GR.
KEGGecj:JW0454.
eco:b0465.

Organism-specific databases

EchoBASEEB3991.
EcoGeneEG14240. kefA.
CMRSearch...

Phylogenomic databases

HOGENOMP77338.
OMAYYTALKL

Enzyme and pathway databases

BioCycEcoCyc:G6255-MON.
ECOL168927:B0465-MON.

Gene expression databases

GenevestigatorP77338.

Family and domain databases

InterProIPR010920. LSM_related_core.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR006686. MscS_channel_CS.
IPR011014. MscS_channel_TM-2.
[Graphical view]
PfamPF00924. MS_channel. 1 hit.
[Graphical view]
PROSITEPS01246. UPF0003. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameKEFA_ECOLI
AccessionPrimary (citable) accession number: P77338
Secondary accession number(s): Q2MBW2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 24, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents