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Protein

Mechanosensitive channel MscK

Gene

mscK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mechanosensitive channel that opens in response to membrane tension and specific ionic conditions. Requires high concentrations of external K+, NH4+, Rb+ or Cs+ to gate. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Forms an ion channel of 1.0 nanosiemens conductance. The channel can remain active for between 30 seconds and over 3 minutes; it does not desensitize upon extended pressure. Its activity is masked in wild-type cells by the MscS channel.3 Publications

GO - Biological processi

  • cellular water homeostasis Source: EcoCyc
  • potassium ion transport Source: UniProtKB-KW
  • transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciEcoCyc:G6255-MONOMER.
ECOL316407:JW0454-MONOMER.

Protein family/group databases

TCDBi1.A.23.1.1. the small conductance mechanosensitive ion channel (mscs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mechanosensitive channel MscK
Alternative name(s):
Potassium efflux system KefA
Gene namesi
Name:mscK
Synonyms:aefA, kefA
Ordered Locus Names:b0465, JW0454
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14240. mscK.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 499PeriplasmicSequence analysisAdd BLAST466
Transmembranei500 – 520HelicalSequence analysisAdd BLAST21
Topological domaini521 – 560CytoplasmicSequence analysisAdd BLAST40
Transmembranei561 – 581HelicalSequence analysisAdd BLAST21
Topological domaini582PeriplasmicSequence analysis1
Transmembranei583 – 603HelicalSequence analysisAdd BLAST21
Topological domaini604 – 634CytoplasmicSequence analysisAdd BLAST31
Transmembranei635 – 655HelicalSequence analysisAdd BLAST21
Topological domaini656 – 657PeriplasmicSequence analysis2
Transmembranei658 – 678HelicalSequence analysisAdd BLAST21
Topological domaini679 – 692CytoplasmicSequence analysisAdd BLAST14
Transmembranei693 – 713HelicalSequence analysisAdd BLAST21
Topological domaini714 – 728PeriplasmicSequence analysisAdd BLAST15
Transmembranei729 – 749HelicalSequence analysisAdd BLAST21
Topological domaini750 – 796CytoplasmicSequence analysisAdd BLAST47
Transmembranei797 – 817HelicalSequence analysisAdd BLAST21
Topological domaini818 – 839PeriplasmicSequence analysisAdd BLAST22
Transmembranei840 – 860HelicalSequence analysisAdd BLAST21
Topological domaini861 – 886CytoplasmicSequence analysisAdd BLAST26
Transmembranei887 – 907HelicalSequence analysisAdd BLAST21
Topological domaini908 – 921PeriplasmicSequence analysisAdd BLAST14
Transmembranei922 – 942HelicalSequence analysisAdd BLAST21
Topological domaini943 – 1120CytoplasmicSequence analysisAdd BLAST178

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi922G → S: Prevents growth in medium containing high levels of potassium in the presence of betaine. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000004336634 – 1120Mechanosensitive channel MscKAdd BLAST1087

Proteomic databases

PaxDbiP77338.
PRIDEiP77338.

Interactioni

Protein-protein interaction databases

BioGridi4259842. 245 interactors.
DIPiDIP-10070N.
IntActiP77338. 3 interactors.
STRINGi511145.b0465.

Structurei

3D structure databases

ProteinModelPortaliP77338.
SMRiP77338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili43 – 98Sequence analysisAdd BLAST56
Coiled coili126 – 266Sequence analysisAdd BLAST141
Coiled coili360 – 422Sequence analysisAdd BLAST63
Coiled coili1057 – 1081Sequence analysisAdd BLAST25

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EGS. Bacteria.
COG3264. LUCA.
HOGENOMiHOG000280842.
InParanoidiP77338.
KOiK05802.
OMAiLHTPKAI.
PhylomeDBiP77338.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR006686. MscS_channel_CS.
IPR011014. MscS_channel_TM-2.
IPR025692. MscS_IM_dom1.
IPR024393. MscS_porin.
[Graphical view]
PfamiPF00924. MS_channel. 1 hit.
PF12795. MscS_porin. 1 hit.
PF12794. MscS_TM. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.
SSF82861. SSF82861. 1 hit.
PROSITEiPS01246. UPF0003. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMFQYYKRS RHFVFSAFIA FVFVLLCQNT AFARASSNGD LPTKADLQAQ
60 70 80 90 100
LDSLNKQKDL SAQDKLVQQD LTDTLATLDK IDRIKEETVQ LRQKVAEAPE
110 120 130 140 150
KMRQATAALT ALSDVDNDEE TRKILSTLSL RQLETRVAQA LDDLQNAQND
160 170 180 190 200
LASYNSQLVS LQTQPERVQN AMYNASQQLQ QIRSRLDGTD VGETALRPSQ
210 220 230 240 250
KVLMQAQQAL LNAEIDQQRK SLEGNTVLQD TLQKQRDYVT ANSARLEHQL
260 270 280 290 300
QLLQEAVNSK RLTLTEKTAQ EAVSPDEAAR IQANPLVKQE LEINQQLSQR
310 320 330 340 350
LITATENGNQ LMQQNIKVKN WLERALQSER NIKEQIAVLK GSLLLSRILY
360 370 380 390 400
QQQQTLPSAD ELENMTNRIA DLRLEQFEVN QQRDALFQSD AFVNKLEEGH
410 420 430 440 450
TNEVNSEVHD ALLQVVDMRR ELLDQLNKQL GNQLMMAINL QINQQQLMSV
460 470 480 490 500
SKNLKSILTQ QIFWVNSNRP MDWDWIKAFP QSLKDEFKSM KITVNWQKAW
510 520 530 540 550
PAVFIAFLAG LPLLLIAGLI HWRLGWLKAY QQKLASAVGS LRNDSQLNTP
560 570 580 590 600
KAILIDLIRA LPVCLIILAV GLILLTMQLN ISELLWSFSK KLAIFWLVFG
610 620 630 640 650
LCWKVLEKNG VAVRHFGMPE QQTSHWRRQI VRISLALLPI HFWSVVAELS
660 670 680 690 700
PLHLMDDVLG QAMIFFNLLL IAFLVWPMCR ESWRDKESHT MRLVTITVLS
710 720 730 740 750
IIPIALMVLT ATGYFYTTLR LAGRWIETVY LVIIWNLLYQ TVLRGLSVAA
760 770 780 790 800
RRIAWRRALA RRQNLVKEGA EGAEPPEEPT IALEQVNQQT LRITMLLMFA
810 820 830 840 850
LFGVMFWAIW SDLITVFSYL DSITLWHYNG TEAGAAVVKN VTMGSLLFAI
860 870 880 890 900
IASMVAWALI RNLPGLLEVL VLSRLNMRQG ASYAITTILN YIIIAVGAMT
910 920 930 940 950
VFGSLGVSWD KLQWLAAALS VGLGFGLQEI FGNFVSGLII LFERPVRIGD
960 970 980 990 1000
TVTIGSFSGT VSKIRIRATT ITDFDRKEVI IPNKAFVTER LINWSLTDTT
1010 1020 1030 1040 1050
TRLVIRLGVA YGSDLEKVRK VLLKAATEHP RVMHEPMPEV FFTAFGASTL
1060 1070 1080 1090 1100
DHELRLYVRE LRDRSRTVDE LNRTIDQLCR ENDINIAFNQ LEVHLHNEKG
1110 1120
DEVTEVKRDY KGDDPTPAVG
Length:1,120
Mass (Da):127,215
Last modified:February 1, 1997 - v1
Checksum:i809895660D2BD444
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07802 Genomic DNA. Translation: CAA69140.1.
U82664 Genomic DNA. Translation: AAB40219.1.
U00096 Genomic DNA. Translation: AAC73567.1.
AP009048 Genomic DNA. Translation: BAE76244.1.
PIRiH64776.
RefSeqiNP_414998.1. NC_000913.3.
WP_000177732.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73567; AAC73567; b0465.
BAE76244; BAE76244; BAE76244.
GeneIDi945132.
KEGGiecj:JW0454.
eco:b0465.
PATRICi32116083. VBIEscCol129921_0483.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07802 Genomic DNA. Translation: CAA69140.1.
U82664 Genomic DNA. Translation: AAB40219.1.
U00096 Genomic DNA. Translation: AAC73567.1.
AP009048 Genomic DNA. Translation: BAE76244.1.
PIRiH64776.
RefSeqiNP_414998.1. NC_000913.3.
WP_000177732.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77338.
SMRiP77338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259842. 245 interactors.
DIPiDIP-10070N.
IntActiP77338. 3 interactors.
STRINGi511145.b0465.

Protein family/group databases

TCDBi1.A.23.1.1. the small conductance mechanosensitive ion channel (mscs) family.

Proteomic databases

PaxDbiP77338.
PRIDEiP77338.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73567; AAC73567; b0465.
BAE76244; BAE76244; BAE76244.
GeneIDi945132.
KEGGiecj:JW0454.
eco:b0465.
PATRICi32116083. VBIEscCol129921_0483.

Organism-specific databases

EchoBASEiEB3991.
EcoGeneiEG14240. mscK.

Phylogenomic databases

eggNOGiENOG4105EGS. Bacteria.
COG3264. LUCA.
HOGENOMiHOG000280842.
InParanoidiP77338.
KOiK05802.
OMAiLHTPKAI.
PhylomeDBiP77338.

Enzyme and pathway databases

BioCyciEcoCyc:G6255-MONOMER.
ECOL316407:JW0454-MONOMER.

Miscellaneous databases

PROiP77338.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR006686. MscS_channel_CS.
IPR011014. MscS_channel_TM-2.
IPR025692. MscS_IM_dom1.
IPR024393. MscS_porin.
[Graphical view]
PfamiPF00924. MS_channel. 1 hit.
PF12795. MscS_porin. 1 hit.
PF12794. MscS_TM. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.
SSF82861. SSF82861. 1 hit.
PROSITEiPS01246. UPF0003. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSCK_ECOLI
AccessioniPrimary (citable) accession number: P77338
Secondary accession number(s): Q2MBW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.