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Protein

Mechanosensitive channel MscK

Gene

mscK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mechanosensitive channel that opens in response to membrane tension and specific ionic conditions. Requires high concentrations of external K+, NH4+, Rb+ or Cs+ to gate. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Forms an ion channel of 1.0 nanosiemens conductance. The channel can remain active for between 30 seconds and over 3 minutes; it does not desensitize upon extended pressure. Its activity is masked in wild-type cells by the MscS channel.3 Publications

GO - Biological processi

  • cellular water homeostasis Source: EcoCyc
  • potassium ion transport Source: UniProtKB-KW
  • transmembrane transport Source: InterPro

Keywordsi

Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

BioCyciEcoCyc:G6255-MONOMER

Protein family/group databases

TCDBi1.A.23.1.1 the small conductance mechanosensitive ion channel (mscs) family

Names & Taxonomyi

Protein namesi
Recommended name:
Mechanosensitive channel MscK
Alternative name(s):
Potassium efflux system KefA
Gene namesi
Name:mscK
Synonyms:aefA, kefA
Ordered Locus Names:b0465, JW0454
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14240 mscK

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 499PeriplasmicSequence analysisAdd BLAST466
Transmembranei500 – 520HelicalSequence analysisAdd BLAST21
Topological domaini521 – 560CytoplasmicSequence analysisAdd BLAST40
Transmembranei561 – 581HelicalSequence analysisAdd BLAST21
Topological domaini582PeriplasmicSequence analysis1
Transmembranei583 – 603HelicalSequence analysisAdd BLAST21
Topological domaini604 – 634CytoplasmicSequence analysisAdd BLAST31
Transmembranei635 – 655HelicalSequence analysisAdd BLAST21
Topological domaini656 – 657PeriplasmicSequence analysis2
Transmembranei658 – 678HelicalSequence analysisAdd BLAST21
Topological domaini679 – 692CytoplasmicSequence analysisAdd BLAST14
Transmembranei693 – 713HelicalSequence analysisAdd BLAST21
Topological domaini714 – 728PeriplasmicSequence analysisAdd BLAST15
Transmembranei729 – 749HelicalSequence analysisAdd BLAST21
Topological domaini750 – 796CytoplasmicSequence analysisAdd BLAST47
Transmembranei797 – 817HelicalSequence analysisAdd BLAST21
Topological domaini818 – 839PeriplasmicSequence analysisAdd BLAST22
Transmembranei840 – 860HelicalSequence analysisAdd BLAST21
Topological domaini861 – 886CytoplasmicSequence analysisAdd BLAST26
Transmembranei887 – 907HelicalSequence analysisAdd BLAST21
Topological domaini908 – 921PeriplasmicSequence analysisAdd BLAST14
Transmembranei922 – 942HelicalSequence analysisAdd BLAST21
Topological domaini943 – 1120CytoplasmicSequence analysisAdd BLAST178

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi922G → S: Prevents growth in medium containing high levels of potassium in the presence of betaine. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000004336634 – 1120Mechanosensitive channel MscKAdd BLAST1087

Proteomic databases

PaxDbiP77338
PRIDEiP77338

Interactioni

Protein-protein interaction databases

BioGridi4259842, 274 interactors
DIPiDIP-10070N
IntActiP77338, 3 interactors
STRINGi316385.ECDH10B_0421

Structurei

3D structure databases

ProteinModelPortaliP77338
SMRiP77338
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili43 – 98Sequence analysisAdd BLAST56
Coiled coili126 – 266Sequence analysisAdd BLAST141
Coiled coili360 – 422Sequence analysisAdd BLAST63
Coiled coili1057 – 1081Sequence analysisAdd BLAST25

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EGS Bacteria
COG3264 LUCA
HOGENOMiHOG000280842
InParanoidiP77338
KOiK05802
OMAiGQLINWS
PhylomeDBiP77338

Family and domain databases

Gene3Di2.30.30.60, 1 hit
InterProiView protein in InterPro
IPR023298 ATPase_P-typ_TM_dom_sf
IPR010920 LSM_dom_sf
IPR011066 MscC_channel_C
IPR006685 MscS_channel
IPR006686 MscS_channel_CS
IPR011014 MscS_channel_TM-2
IPR023408 MscS_dom_sf
IPR025692 MscS_IM_dom1
IPR024393 MscS_porin
PfamiView protein in Pfam
PF00924 MS_channel, 1 hit
PF12795 MscS_porin, 1 hit
PF12794 MscS_TM, 1 hit
SUPFAMiSSF50182 SSF50182, 1 hit
SSF81665 SSF81665, 3 hits
SSF82689 SSF82689, 1 hit
SSF82861 SSF82861, 1 hit
PROSITEiView protein in PROSITE
PS01246 UPF0003, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMFQYYKRS RHFVFSAFIA FVFVLLCQNT AFARASSNGD LPTKADLQAQ
60 70 80 90 100
LDSLNKQKDL SAQDKLVQQD LTDTLATLDK IDRIKEETVQ LRQKVAEAPE
110 120 130 140 150
KMRQATAALT ALSDVDNDEE TRKILSTLSL RQLETRVAQA LDDLQNAQND
160 170 180 190 200
LASYNSQLVS LQTQPERVQN AMYNASQQLQ QIRSRLDGTD VGETALRPSQ
210 220 230 240 250
KVLMQAQQAL LNAEIDQQRK SLEGNTVLQD TLQKQRDYVT ANSARLEHQL
260 270 280 290 300
QLLQEAVNSK RLTLTEKTAQ EAVSPDEAAR IQANPLVKQE LEINQQLSQR
310 320 330 340 350
LITATENGNQ LMQQNIKVKN WLERALQSER NIKEQIAVLK GSLLLSRILY
360 370 380 390 400
QQQQTLPSAD ELENMTNRIA DLRLEQFEVN QQRDALFQSD AFVNKLEEGH
410 420 430 440 450
TNEVNSEVHD ALLQVVDMRR ELLDQLNKQL GNQLMMAINL QINQQQLMSV
460 470 480 490 500
SKNLKSILTQ QIFWVNSNRP MDWDWIKAFP QSLKDEFKSM KITVNWQKAW
510 520 530 540 550
PAVFIAFLAG LPLLLIAGLI HWRLGWLKAY QQKLASAVGS LRNDSQLNTP
560 570 580 590 600
KAILIDLIRA LPVCLIILAV GLILLTMQLN ISELLWSFSK KLAIFWLVFG
610 620 630 640 650
LCWKVLEKNG VAVRHFGMPE QQTSHWRRQI VRISLALLPI HFWSVVAELS
660 670 680 690 700
PLHLMDDVLG QAMIFFNLLL IAFLVWPMCR ESWRDKESHT MRLVTITVLS
710 720 730 740 750
IIPIALMVLT ATGYFYTTLR LAGRWIETVY LVIIWNLLYQ TVLRGLSVAA
760 770 780 790 800
RRIAWRRALA RRQNLVKEGA EGAEPPEEPT IALEQVNQQT LRITMLLMFA
810 820 830 840 850
LFGVMFWAIW SDLITVFSYL DSITLWHYNG TEAGAAVVKN VTMGSLLFAI
860 870 880 890 900
IASMVAWALI RNLPGLLEVL VLSRLNMRQG ASYAITTILN YIIIAVGAMT
910 920 930 940 950
VFGSLGVSWD KLQWLAAALS VGLGFGLQEI FGNFVSGLII LFERPVRIGD
960 970 980 990 1000
TVTIGSFSGT VSKIRIRATT ITDFDRKEVI IPNKAFVTER LINWSLTDTT
1010 1020 1030 1040 1050
TRLVIRLGVA YGSDLEKVRK VLLKAATEHP RVMHEPMPEV FFTAFGASTL
1060 1070 1080 1090 1100
DHELRLYVRE LRDRSRTVDE LNRTIDQLCR ENDINIAFNQ LEVHLHNEKG
1110 1120
DEVTEVKRDY KGDDPTPAVG
Length:1,120
Mass (Da):127,215
Last modified:February 1, 1997 - v1
Checksum:i809895660D2BD444
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07802 Genomic DNA Translation: CAA69140.1
U82664 Genomic DNA Translation: AAB40219.1
U00096 Genomic DNA Translation: AAC73567.1
AP009048 Genomic DNA Translation: BAE76244.1
PIRiH64776
RefSeqiNP_414998.1, NC_000913.3
WP_000177732.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73567; AAC73567; b0465
BAE76244; BAE76244; BAE76244
GeneIDi945132
KEGGiecj:JW0454
eco:b0465
PATRICifig|1411691.4.peg.1811

Similar proteinsi

Entry informationi

Entry nameiMSCK_ECOLI
AccessioniPrimary (citable) accession number: P77338
Secondary accession number(s): Q2MBW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health