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Protein

Hydrogenase-4 component G

Gene

hyfG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

[4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-150.
ECOL316407:JW2472-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-4 component G (EC:1.-.-.-)
Gene namesi
Name:hyfG
Ordered Locus Names:b2487, JW2472
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14215. hyfG.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Hydrogenase-4 component GPRO_0000118626Add
BLAST

Proteomic databases

PaxDbiP77329.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F53EBI-548413,EBI-543750

Protein-protein interaction databases

BioGridi4260765. 3 interactions.
DIPiDIP-9989N.
IntActiP77329. 7 interactions.
MINTiMINT-1310624.
STRINGi511145.b2487.

Structurei

3D structure databases

ProteinModelPortaliP77329.
SMRiP77329. Positions 186-523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105CQV. Bacteria.
COG3261. LUCA.
COG3262. LUCA.
HOGENOMiHOG000228262.
InParanoidiP77329.
KOiK12142.
OMAiRGENVHW.
OrthoDBiEOG6HF5VV.
PhylomeDBiP77329.

Family and domain databases

Gene3Di1.10.645.10. 2 hits.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR001501. Ni-dep_hyd_lsu.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
PF00346. Complex1_49kDa. 2 hits.
PF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56762. SSF56762. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77329-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVNSSSNRG EAILAALKTQ FPGAVLDEER QTPEQVTITV KINLLPDVVQ
60 70 80 90 100
YLYYQHDGWL PVLFGNDERT LNGHYAVYYA LSMEGAEKCW IVVKALVDAD
110 120 130 140 150
SREFPSVTPR VPAAVWGERE IRDMYGLIPV GLPDQRRLVL PDDWPEDMHP
160 170 180 190 200
LRKDAMDYRL RPEPTTDSET YPFINEGNSD ARVIPVGPLH ITSDEPGHFR
210 220 230 240 250
LFVDGEQIVD ADYRLFYVHR GMEKLAETRM GYNEVTFLSD RVCGICGFAH
260 270 280 290 300
SVAYTNSVEN ALGIEVPQRA HTIRSILLEV ERLHSHLLNL GLSCHFVGFD
310 320 330 340 350
TGFMQFFRVR EKSMTMAELL IGSRKTYGLN LIGGVRRDIL KEQRLQTLKL
360 370 380 390 400
VREMRADVSE LVEMLLATPN MEQRTQGIGI LDRQIARDLR FDHPYADYGN
410 420 430 440 450
IPKTLFTFTG GDVFSRVMVR VKETFDSLAM LEFALDNMPD TPLLTEGFSY
460 470 480 490 500
KPHAFALGFV EAPRGEDVHW SMLGDNQKLF RWRCRAATYA NWPVLRYMLR
510 520 530 540 550
GNTVSDAPLI IGSLDPCYSC TDRVTLVDVR KRQSKTVPYK EIERYGIDRN

RSPLK
Length:555
Mass (Da):63,383
Last modified:February 1, 1997 - v1
Checksum:iA5D410E6E74C05CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88569.1.
U00096 Genomic DNA. Translation: AAC75540.1.
AP009048 Genomic DNA. Translation: BAA16375.2.
PIRiF65024.
RefSeqiNP_416982.1. NC_000913.3.
WP_001102321.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75540; AAC75540; b2487.
BAA16375; BAA16375; BAA16375.
GeneIDi946964.
KEGGiecj:JW2472.
eco:b2487.
PATRICi32120361. VBIEscCol129921_2583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88569.1.
U00096 Genomic DNA. Translation: AAC75540.1.
AP009048 Genomic DNA. Translation: BAA16375.2.
PIRiF65024.
RefSeqiNP_416982.1. NC_000913.3.
WP_001102321.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77329.
SMRiP77329. Positions 186-523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260765. 3 interactions.
DIPiDIP-9989N.
IntActiP77329. 7 interactions.
MINTiMINT-1310624.
STRINGi511145.b2487.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP77329.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75540; AAC75540; b2487.
BAA16375; BAA16375; BAA16375.
GeneIDi946964.
KEGGiecj:JW2472.
eco:b2487.
PATRICi32120361. VBIEscCol129921_2583.

Organism-specific databases

EchoBASEiEB3967.
EcoGeneiEG14215. hyfG.

Phylogenomic databases

eggNOGiENOG4105CQV. Bacteria.
COG3261. LUCA.
COG3262. LUCA.
HOGENOMiHOG000228262.
InParanoidiP77329.
KOiK12142.
OMAiRGENVHW.
OrthoDBiEOG6HF5VV.
PhylomeDBiP77329.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-150.
ECOL316407:JW2472-MONOMER.

Miscellaneous databases

PROiP77329.

Family and domain databases

Gene3Di1.10.645.10. 2 hits.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR001501. Ni-dep_hyd_lsu.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
PF00346. Complex1_49kDa. 2 hits.
PF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56762. SSF56762. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Andrews S.C., McClay J., Ambler A., Quail M., Berks B.C., Guest J.R.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiHYFG_ECOLI
AccessioniPrimary (citable) accession number: P77329
Secondary accession number(s): P76974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.