Hydrogenase-4 component G - Escherichia coli (strain K12)

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P77329 (HYFG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Hydrogenase-4 component G
EC=1.-.-.-

Gene names

Name:	hyfG
Ordered Locus Names:	b2487, JW2472

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Binds 1 4Fe-4S cluster.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro nickel cation binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	3	EBI-548413,EBI-543750

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 555

555

Hydrogenase-4 component G

PRO_0000118626

Sequences

Sequence

Length

Mass (Da)

Tools

P77329 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: A5D410E6E74C05CD
Show »

FASTA

555

63,383

        10         20         30         40         50         60 
MNVNSSSNRG EAILAALKTQ FPGAVLDEER QTPEQVTITV KINLLPDVVQ YLYYQHDGWL 

        70         80         90        100        110        120 
PVLFGNDERT LNGHYAVYYA LSMEGAEKCW IVVKALVDAD SREFPSVTPR VPAAVWGERE 

       130        140        150        160        170        180 
IRDMYGLIPV GLPDQRRLVL PDDWPEDMHP LRKDAMDYRL RPEPTTDSET YPFINEGNSD 

       190        200        210        220        230        240 
ARVIPVGPLH ITSDEPGHFR LFVDGEQIVD ADYRLFYVHR GMEKLAETRM GYNEVTFLSD 

       250        260        270        280        290        300 
RVCGICGFAH SVAYTNSVEN ALGIEVPQRA HTIRSILLEV ERLHSHLLNL GLSCHFVGFD 

       310        320        330        340        350        360 
TGFMQFFRVR EKSMTMAELL IGSRKTYGLN LIGGVRRDIL KEQRLQTLKL VREMRADVSE 

       370        380        390        400        410        420 
LVEMLLATPN MEQRTQGIGI LDRQIARDLR FDHPYADYGN IPKTLFTFTG GDVFSRVMVR 

       430        440        450        460        470        480 
VKETFDSLAM LEFALDNMPD TPLLTEGFSY KPHAFALGFV EAPRGEDVHW SMLGDNQKLF 

       490        500        510        520        530        540 
RWRCRAATYA NWPVLRYMLR GNTVSDAPLI IGSLDPCYSC TDRVTLVDVR KRQSKTVPYK 

       550 
EIERYGIDRN RSPLK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Andrews S.C., McClay J., Ambler A., Quail M., Berks B.C., Guest J.R. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63654 Genomic DNA. Translation: AAB88569.1. U00096 Genomic DNA. Translation: AAC75540.1. AP009048 Genomic DNA. Translation: BAA16375.2.
PIR	F65024.
RefSeq	NP_416982.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P77329.
SMR	P77329. Positions 9-136, 181-523.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9989N.
IntAct	P77329. 7 interactions.
MINT	MINT-1310624.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001714; EBESCP00000001714; EBESCG00000001412. EBESCT00000018243; EBESCP00000017534; EBESCG00000017298.
GeneID	946964.
GenomeReviews	Gene locus JW2472 in contig AP009048_GR. Gene locus b2487 in contig U00096_GR.
KEGG	ecj:JW2472. eco:b2487.
PATRIC	32120361. VBIEscCol129921_2583.
Organism-specific databases
EchoBASE	EB3967.
EcoGene	EG14215. hyfG.
Phylogenomic databases
eggNOG	COG3261.
GeneTree	EBGT00050000010529.
HOGENOM	HBG459705.
OMA	YSCPIIG.
PhylomeDB	P77329.
ProtClustDB	CLSK880407.
Enzyme and pathway databases
BioCyc	EcoCyc:MONOMER0-150.
Gene expression databases
Genevestigator	P77329.
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR001501. Ni-dep_hyd_lsu. [Graphical view]
KO	K12142.
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 2 hits. PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
ProDom	PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYFG_ECOLI

Accession

Primary (citable) accession number: P77329
Secondary accession number(s): P76974

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents