Reviewed, UniProtKB/Swiss-Prot P77329 (HYFG_ECOLI)

Names and origin

Protein names

Recommended name:
    Hydrogenase-4 component G
    EC=1.-.-.-

Gene names

Name:	hyfG
Ordered Locus Names:	b2487, JW2472

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Binds 1 4Fe-4S cluster.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	2	EBI-548413,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Hydrogenase-4 component G		PRO_0000118626

Sequences

	Sequence		Length	Mass (Da)
	P77329-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: A5D410E6E74C05CD	FASTA	555	63,383
	10 20 30 40 50 60 MNVNSSSNRG EAILAALKTQ FPGAVLDEER QTPEQVTITV KINLLPDVVQ YLYYQHDGWL 70 80 90 100 110 120 PVLFGNDERT LNGHYAVYYA LSMEGAEKCW IVVKALVDAD SREFPSVTPR VPAAVWGERE 130 140 150 160 170 180 IRDMYGLIPV GLPDQRRLVL PDDWPEDMHP LRKDAMDYRL RPEPTTDSET YPFINEGNSD 190 200 210 220 230 240 ARVIPVGPLH ITSDEPGHFR LFVDGEQIVD ADYRLFYVHR GMEKLAETRM GYNEVTFLSD 250 260 270 280 290 300 RVCGICGFAH SVAYTNSVEN ALGIEVPQRA HTIRSILLEV ERLHSHLLNL GLSCHFVGFD 310 320 330 340 350 360 TGFMQFFRVR EKSMTMAELL IGSRKTYGLN LIGGVRRDIL KEQRLQTLKL VREMRADVSE 370 380 390 400 410 420 LVEMLLATPN MEQRTQGIGI LDRQIARDLR FDHPYADYGN IPKTLFTFTG GDVFSRVMVR 430 440 450 460 470 480 VKETFDSLAM LEFALDNMPD TPLLTEGFSY KPHAFALGFV EAPRGEDVHW SMLGDNQKLF 490 500 510 520 530 540 RWRCRAATYA NWPVLRYMLR GNTVSDAPLI IGSLDPCYSC TDRVTLVDVR KRQSKTVPYK 550 EIERYGIDRN RSPLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Andrews S.C., McClay J., Ambler A., Quail M., Berks B.C., Guest J.R. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63654 Genomic DNA. Translation: AAB88569.1. U00096 Genomic DNA. Translation: AAC75540.1. AP009048 Genomic DNA. Translation: BAA16375.2.
PIR	F65024.
RefSeq	AP_003073.1. NP_416982.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9989N.
IntAct	P77329. 7 interactions.
Genome annotation databases
GeneID	946964.
GenomeReviews	Gene locus JW2472 in contig AP009048_GR. Gene locus b2487 in contig U00096_GR.
KEGG	ecj:JW2472. eco:b2487.
Organism-specific databases
EchoBASE	EB3967.
EcoGene	EG14215. hyfG.
CMR	Search...
Phylogenomic databases
HOGENOM	P77329.
OMA	P77329. SCDPCFT.
Enzyme and pathway databases
BioCyc	EcoCyc:MON0-150.
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_su-49kDa_CS. IPR001501. Ni-dep_hyd_lsu. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 2 hits. PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYFG_ECOLI

Accession

Primary (citable) accession number: P77329
Secondary accession number(s): P76974

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)