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Protein

Putative xanthine dehydrogenase YagS FAD-binding subunit

Gene

yagS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei212 – 2121FAD; via amide nitrogen and carbonyl oxygenSequence Analysis
Binding sitei227 – 2271FADSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338FADSequence Analysis
Nucleotide bindingi107 – 1115FADSequence Analysis

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: EcoCyc
  2. oxidoreductase activity, acting on the aldehyde or oxo group of donors Source: EcoCyc
  3. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
  4. xanthine dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. purine nucleobase metabolic process Source: UniProtKB-KW
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism, Purine salvage

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G6156-MONOMER.
ECOL316407:JW0279-MONOMER.
MetaCyc:G6156-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative xanthine dehydrogenase YagS FAD-binding subunit (EC:1.17.1.4)
Gene namesi
Name:yagS
Ordered Locus Names:b0285, JW0279
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13558. yagS.

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Putative xanthine dehydrogenase YagS FAD-binding subunitPRO_0000166094Add
BLAST

Proteomic databases

PRIDEiP77324.

Expressioni

Gene expression databases

GenevestigatoriP77324.

Interactioni

Subunit structurei

Heterotrimer of YagR, YagS and YagT.Curated

Protein-protein interaction databases

IntActiP77324. 7 interactions.
STRINGi511145.b0285.

Structurei

3D structure databases

ProteinModelPortaliP77324.
SMRiP77324. Positions 3-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 223223FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1319.
HOGENOMiHOG000244727.
InParanoidiP77324.
KOiK11178.
OMAiIGGFSRQ.
OrthoDBiEOG66TG3V.
PhylomeDBiP77324.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 2 hits.
InterProiIPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamiPF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
[Graphical view]
SMARTiSM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAFTYERVN TPAEAALSAQ RVPGAKFIAG GTNLLDLMKL EIETPTHLID
60 70 80 90 100
VNGLGLDKIE VTDAGGLRIG ALVRNTDLAA HERVRRDYAV LSRALLAGAS
110 120 130 140 150
GQLRNQATTA GNLLQRTRCP YFYDTNQPCN KRLPGSGCAA LEGFSRQHAV
160 170 180 190 200
VGVSEACIAT HPSDMAVAMR LLDAVVETIT PEGKTRSITL ADFYHPPGKT
210 220 230 240 250
PHIETALLPG ELIVAVTLPP PLGGKHIYRK VRDRASYAFA LVSVAAIIQP
260 270 280 290 300
DGSGRVALGG VAHKPWRIEA ADAQLSQGAQ AVYDTLFASA HPTAENTFKL
310
LLAKRTLASV LAEARAQA
Length:318
Mass (Da):33,858
Last modified:January 31, 1997 - v1
Checksum:i7D51C2B30C9BB222
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18014.1.
U00096 Genomic DNA. Translation: AAC73388.1.
AP009048 Genomic DNA. Translation: BAE76069.1.
PIRiE64754.
RefSeqiNP_414819.1. NC_000913.3.
YP_488581.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73388; AAC73388; b0285.
BAE76069; BAE76069; BAE76069.
GeneIDi12930791.
945710.
KEGGiecj:Y75_p0277.
eco:b0285.
PATRICi32115693. VBIEscCol129921_0289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18014.1.
U00096 Genomic DNA. Translation: AAC73388.1.
AP009048 Genomic DNA. Translation: BAE76069.1.
PIRiE64754.
RefSeqiNP_414819.1. NC_000913.3.
YP_488581.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP77324.
SMRiP77324. Positions 3-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP77324. 7 interactions.
STRINGi511145.b0285.

Proteomic databases

PRIDEiP77324.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73388; AAC73388; b0285.
BAE76069; BAE76069; BAE76069.
GeneIDi12930791.
945710.
KEGGiecj:Y75_p0277.
eco:b0285.
PATRICi32115693. VBIEscCol129921_0289.

Organism-specific databases

EchoBASEiEB3328.
EcoGeneiEG13558. yagS.

Phylogenomic databases

eggNOGiCOG1319.
HOGENOMiHOG000244727.
InParanoidiP77324.
KOiK11178.
OMAiIGGFSRQ.
OrthoDBiEOG66TG3V.
PhylomeDBiP77324.

Enzyme and pathway databases

BioCyciEcoCyc:G6156-MONOMER.
ECOL316407:JW0279-MONOMER.
MetaCyc:G6156-MONOMER.

Miscellaneous databases

PROiP77324.

Gene expression databases

GenevestigatoriP77324.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 2 hits.
InterProiIPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamiPF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
[Graphical view]
SMARTiSM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiYAGS_ECOLI
AccessioniPrimary (citable) accession number: P77324
Secondary accession number(s): Q2MCD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: January 31, 1997
Last modified: January 6, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.