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P77324 (YAGS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative xanthine dehydrogenase YagS FAD-binding subunit

EC=1.17.1.4
Gene names
Name:yagS
Ordered Locus Names:b0285, JW0279
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Xanthine + NAD+ + H2O = urate + NADH.

Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactor

FAD By similarity.

Subunit structure

Heterotrimer of YagR, YagS and YagT Potential.

Sequence similarities

Contains 1 FAD-binding PCMH-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Putative xanthine dehydrogenase YagS FAD-binding subunit
PRO_0000166094

Regions

Domain1 – 223223FAD-binding PCMH-type
Nucleotide binding26 – 338FAD Potential
Nucleotide binding107 – 1115FAD Potential

Sites

Binding site2121FAD; via amide nitrogen and carbonyl oxygen Potential
Binding site2271FAD Potential

Sequences

Sequence LengthMass (Da)Tools
P77324 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 7D51C2B30C9BB222

FASTA31833,858
        10         20         30         40         50         60 
MKAFTYERVN TPAEAALSAQ RVPGAKFIAG GTNLLDLMKL EIETPTHLID VNGLGLDKIE 

        70         80         90        100        110        120 
VTDAGGLRIG ALVRNTDLAA HERVRRDYAV LSRALLAGAS GQLRNQATTA GNLLQRTRCP 

       130        140        150        160        170        180 
YFYDTNQPCN KRLPGSGCAA LEGFSRQHAV VGVSEACIAT HPSDMAVAMR LLDAVVETIT 

       190        200        210        220        230        240 
PEGKTRSITL ADFYHPPGKT PHIETALLPG ELIVAVTLPP PLGGKHIYRK VRDRASYAFA 

       250        260        270        280        290        300 
LVSVAAIIQP DGSGRVALGG VAHKPWRIEA ADAQLSQGAQ AVYDTLFASA HPTAENTFKL 

       310 
LLAKRTLASV LAEARAQA 

« Hide

References

[1]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U73857 Genomic DNA. Translation: AAB18014.1.
U00096 Genomic DNA. Translation: AAC73388.1.
AP009048 Genomic DNA. Translation: BAE76069.1.
PIRE64754.
RefSeqNP_414819.1. NC_000913.3.
YP_488581.1. NC_007779.1.

3D structure databases

ProteinModelPortalP77324.
SMRP77324. Positions 3-271.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP77324. 7 interactions.
STRING511145.b0285.

Proteomic databases

PRIDEP77324.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73388; AAC73388; b0285.
BAE76069; BAE76069; BAE76069.
GeneID12930791.
945710.
KEGGecj:Y75_p0277.
eco:b0285.
PATRIC32115693. VBIEscCol129921_0289.

Organism-specific databases

EchoBASEEB3328.
EcoGeneEG13558. yagS.

Phylogenomic databases

eggNOGCOG1319.
HOGENOMHOG000244727.
KOK11178.
OMAPCNKRRP.
OrthoDBEOG66TG3V.
PhylomeDBP77324.

Enzyme and pathway databases

BioCycEcoCyc:G6156-MONOMER.
ECOL316407:JW0279-MONOMER.
MetaCyc:G6156-MONOMER.

Gene expression databases

GenevestigatorP77324.

Family and domain databases

Gene3D3.30.43.10. 1 hit.
3.30.465.10. 2 hits.
InterProIPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamPF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
[Graphical view]
SMARTSM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMSSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP77324.

Entry information

Entry nameYAGS_ECOLI
AccessionPrimary (citable) accession number: P77324
Secondary accession number(s): Q2MCD7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene