SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P77324

- YAGS_ECOLI

UniProt

P77324 - YAGS_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Putative xanthine dehydrogenase YagS FAD-binding subunit
Gene
yagS, b0285, JW0279
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

FAD By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei212 – 2121FAD; via amide nitrogen and carbonyl oxygen Reviewed prediction
Binding sitei227 – 2271FAD Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338FAD Reviewed prediction
Nucleotide bindingi107 – 1115FAD Reviewed prediction

GO - Molecular functioni

  1. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
  2. flavin adenine dinucleotide binding Source: EcoCyc
  3. oxidoreductase activity, acting on the aldehyde or oxo group of donors Source: EcoCyc
  4. protein binding Source: EcoliWiki
  5. xanthine dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. purine nucleobase metabolic process Source: UniProtKB-KW
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism, Purine salvage

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G6156-MONOMER.
ECOL316407:JW0279-MONOMER.
MetaCyc:G6156-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative xanthine dehydrogenase YagS FAD-binding subunit (EC:1.17.1.4)
Gene namesi
Name:yagS
Ordered Locus Names:b0285, JW0279
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13558. yagS.

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Putative xanthine dehydrogenase YagS FAD-binding subunit
PRO_0000166094Add
BLAST

Proteomic databases

PRIDEiP77324.

Expressioni

Gene expression databases

GenevestigatoriP77324.

Interactioni

Subunit structurei

Heterotrimer of YagR, YagS and YagT Reviewed prediction.

Protein-protein interaction databases

IntActiP77324. 7 interactions.
STRINGi511145.b0285.

Structurei

3D structure databases

ProteinModelPortaliP77324.
SMRiP77324. Positions 3-271.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 223223FAD-binding PCMH-type
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1319.
HOGENOMiHOG000244727.
KOiK11178.
OMAiPCNKRRP.
OrthoDBiEOG66TG3V.
PhylomeDBiP77324.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 2 hits.
InterProiIPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamiPF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
[Graphical view]
SMARTiSM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77324-1 [UniParc]FASTAAdd to Basket

« Hide

MKAFTYERVN TPAEAALSAQ RVPGAKFIAG GTNLLDLMKL EIETPTHLID    50
VNGLGLDKIE VTDAGGLRIG ALVRNTDLAA HERVRRDYAV LSRALLAGAS 100
GQLRNQATTA GNLLQRTRCP YFYDTNQPCN KRLPGSGCAA LEGFSRQHAV 150
VGVSEACIAT HPSDMAVAMR LLDAVVETIT PEGKTRSITL ADFYHPPGKT 200
PHIETALLPG ELIVAVTLPP PLGGKHIYRK VRDRASYAFA LVSVAAIIQP 250
DGSGRVALGG VAHKPWRIEA ADAQLSQGAQ AVYDTLFASA HPTAENTFKL 300
LLAKRTLASV LAEARAQA 318
Length:318
Mass (Da):33,858
Last modified:February 1, 1997 - v1
Checksum:i7D51C2B30C9BB222
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73857 Genomic DNA. Translation: AAB18014.1.
U00096 Genomic DNA. Translation: AAC73388.1.
AP009048 Genomic DNA. Translation: BAE76069.1.
PIRiE64754.
RefSeqiNP_414819.1. NC_000913.3.
YP_488581.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73388; AAC73388; b0285.
BAE76069; BAE76069; BAE76069.
GeneIDi12930791.
945710.
KEGGiecj:Y75_p0277.
eco:b0285.
PATRICi32115693. VBIEscCol129921_0289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73857 Genomic DNA. Translation: AAB18014.1 .
U00096 Genomic DNA. Translation: AAC73388.1 .
AP009048 Genomic DNA. Translation: BAE76069.1 .
PIRi E64754.
RefSeqi NP_414819.1. NC_000913.3.
YP_488581.1. NC_007779.1.

3D structure databases

ProteinModelPortali P77324.
SMRi P77324. Positions 3-271.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P77324. 7 interactions.
STRINGi 511145.b0285.

Proteomic databases

PRIDEi P77324.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73388 ; AAC73388 ; b0285 .
BAE76069 ; BAE76069 ; BAE76069 .
GeneIDi 12930791.
945710.
KEGGi ecj:Y75_p0277.
eco:b0285.
PATRICi 32115693. VBIEscCol129921_0289.

Organism-specific databases

EchoBASEi EB3328.
EcoGenei EG13558. yagS.

Phylogenomic databases

eggNOGi COG1319.
HOGENOMi HOG000244727.
KOi K11178.
OMAi PCNKRRP.
OrthoDBi EOG66TG3V.
PhylomeDBi P77324.

Enzyme and pathway databases

BioCyci EcoCyc:G6156-MONOMER.
ECOL316407:JW0279-MONOMER.
MetaCyc:G6156-MONOMER.

Miscellaneous databases

PROi P77324.

Gene expression databases

Genevestigatori P77324.

Family and domain databases

Gene3Di 3.30.43.10. 1 hit.
3.30.465.10. 2 hits.
InterProi IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view ]
Pfami PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
[Graphical view ]
SMARTi SM01092. CO_deh_flav_C. 1 hit.
[Graphical view ]
SUPFAMi SSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEi PS51387. FAD_PCMH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiYAGS_ECOLI
AccessioniPrimary (citable) accession number: P77324
Secondary accession number(s): Q2MCD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi