Putative xanthine dehydrogenase YagS FAD-binding subunit

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P77324 (YAGS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative xanthine dehydrogenase YagS FAD-binding subunit
EC=1.17.1.4

Gene names

Name:	yagS
Ordered Locus Names:	b0285, JW0279

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Xanthine + NAD⁺ + H₂O = urate + NADH. Hypoxanthine + NAD⁺ + H₂O = xanthine + NADH.
Cofactor	FAD By similarity.
Subunit structure	Heterotrimer of YagR, YagS and YagT Potential.
Sequence similarities	Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Purine metabolism Purine salvage
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Non-traceable author statement. Source: EcoCyc
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: InterPro aldehyde ferredoxin oxidoreductase activity Inferred from direct assay. Source: EcoCyc flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro xanthine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 318

318

Putative xanthine dehydrogenase YagS FAD-binding subunit

PRO_0000166094

Regions

Domain

1 – 223

223

FAD-binding PCMH-type

Nucleotide binding

26 – 33

FAD Potential

Nucleotide binding

107 – 111

FAD Potential

Sites

Binding site

212

FAD; via amide nitrogen and carbonyl oxygen Potential

Binding site

227

FAD Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P77324 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 7D51C2B30C9BB222
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FASTA

318

33,858

        10         20         30         40         50         60 
MKAFTYERVN TPAEAALSAQ RVPGAKFIAG GTNLLDLMKL EIETPTHLID VNGLGLDKIE 

        70         80         90        100        110        120 
VTDAGGLRIG ALVRNTDLAA HERVRRDYAV LSRALLAGAS GQLRNQATTA GNLLQRTRCP 

       130        140        150        160        170        180 
YFYDTNQPCN KRLPGSGCAA LEGFSRQHAV VGVSEACIAT HPSDMAVAMR LLDAVVETIT 

       190        200        210        220        230        240 
PEGKTRSITL ADFYHPPGKT PHIETALLPG ELIVAVTLPP PLGGKHIYRK VRDRASYAFA 

       250        260        270        280        290        300 
LVSVAAIIQP DGSGRVALGG VAHKPWRIEA ADAQLSQGAQ AVYDTLFASA HPTAENTFKL 

       310 
LLAKRTLASV LAEARAQA

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References

[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73857 Genomic DNA. Translation: AAB18014.1. U00096 Genomic DNA. Translation: AAC73388.1. AP009048 Genomic DNA. Translation: BAE76069.1.
PIR	E64754.
RefSeq	NP_414819.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P77324.
SMR	P77324. Positions 1-316.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77324. 7 interactions.
Proteomic databases
PRIDE	P77324.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004272; EBESCP00000004272; EBESCG00000003483. EBESCT00000015398; EBESCP00000014689; EBESCG00000014458.
GeneID	945710.
GenomeReviews	Gene locus JW0279 in contig AP009048_GR. Gene locus b0285 in contig U00096_GR.
KEGG	ecj:JW0279. eco:b0285.
PATRIC	32115693. VBIEscCol129921_0289.
Organism-specific databases
EchoBASE	EB3328.
EcoGene	EG13558. yagS.
Phylogenomic databases
eggNOG	COG1319.
GeneTree	EBGT00050000011138.
HOGENOM	HBG695787.
OMA	DMAIAMR.
PhylomeDB	P77324.
ProtClustDB	CLSK879612.
Enzyme and pathway databases
BioCyc	EcoCyc:G6156-MONOMER. MetaCyc:G6156-MONOMER.
Gene expression databases
Genevestigator	P77324.
Family and domain databases
InterPro	IPR005107. CO_DH_flav_C. IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR002346. Mopterin_DH_FAD-bd. [Graphical view]
Gene3D	G3DSA:3.30.390.50. CO_DH_flav_C. 1 hit. G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 2 hits. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit.
KO	K11178.
Pfam	PF03450. CO_deh_flav_C. 1 hit. PF00941. FAD_binding_5. 1 hit. [Graphical view]
SMART	SM01092. CO_deh_flav_C. 1 hit. [Graphical view]
SUPFAM	SSF55447. CO_deh_flav_C. 1 hit. SSF56176. FAD-binding_2. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAGS_ECOLI

Accession

Primary (citable) accession number: P77324
Secondary accession number(s): Q2MCD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents