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P77324

- YAGS_ECOLI

UniProt

P77324 - YAGS_ECOLI

Protein

Putative xanthine dehydrogenase YagS FAD-binding subunit

Gene

yagS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Xanthine + NAD+ + H2O = urate + NADH.
    Hypoxanthine + NAD+ + H2O = xanthine + NADH.

    Cofactori

    FAD.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei212 – 2121FAD; via amide nitrogen and carbonyl oxygenSequence Analysis
    Binding sitei227 – 2271FADSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 338FADSequence Analysis
    Nucleotide bindingi107 – 1115FADSequence Analysis

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: EcoCyc
    2. oxidoreductase activity, acting on the aldehyde or oxo group of donors Source: EcoCyc
    3. protein binding Source: EcoliWiki
    4. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
    5. xanthine dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. purine nucleobase metabolic process Source: UniProtKB-KW
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Purine metabolism, Purine salvage

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Enzyme and pathway databases

    BioCyciEcoCyc:G6156-MONOMER.
    ECOL316407:JW0279-MONOMER.
    MetaCyc:G6156-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative xanthine dehydrogenase YagS FAD-binding subunit (EC:1.17.1.4)
    Gene namesi
    Name:yagS
    Ordered Locus Names:b0285, JW0279
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13558. yagS.

    Subcellular locationi

    GO - Cellular componenti

    1. periplasmic space Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 318318Putative xanthine dehydrogenase YagS FAD-binding subunitPRO_0000166094Add
    BLAST

    Proteomic databases

    PRIDEiP77324.

    Expressioni

    Gene expression databases

    GenevestigatoriP77324.

    Interactioni

    Subunit structurei

    Heterotrimer of YagR, YagS and YagT.Curated

    Protein-protein interaction databases

    IntActiP77324. 7 interactions.
    STRINGi511145.b0285.

    Structurei

    3D structure databases

    ProteinModelPortaliP77324.
    SMRiP77324. Positions 3-271.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 223223FAD-binding PCMH-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1319.
    HOGENOMiHOG000244727.
    KOiK11178.
    OMAiPCNKRRP.
    OrthoDBiEOG66TG3V.
    PhylomeDBiP77324.

    Family and domain databases

    Gene3Di3.30.43.10. 1 hit.
    3.30.465.10. 2 hits.
    InterProiIPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    [Graphical view]
    PfamiPF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    [Graphical view]
    SMARTiSM01092. CO_deh_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF55447. SSF55447. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEiPS51387. FAD_PCMH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P77324-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKAFTYERVN TPAEAALSAQ RVPGAKFIAG GTNLLDLMKL EIETPTHLID    50
    VNGLGLDKIE VTDAGGLRIG ALVRNTDLAA HERVRRDYAV LSRALLAGAS 100
    GQLRNQATTA GNLLQRTRCP YFYDTNQPCN KRLPGSGCAA LEGFSRQHAV 150
    VGVSEACIAT HPSDMAVAMR LLDAVVETIT PEGKTRSITL ADFYHPPGKT 200
    PHIETALLPG ELIVAVTLPP PLGGKHIYRK VRDRASYAFA LVSVAAIIQP 250
    DGSGRVALGG VAHKPWRIEA ADAQLSQGAQ AVYDTLFASA HPTAENTFKL 300
    LLAKRTLASV LAEARAQA 318
    Length:318
    Mass (Da):33,858
    Last modified:February 1, 1997 - v1
    Checksum:i7D51C2B30C9BB222
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73857 Genomic DNA. Translation: AAB18014.1.
    U00096 Genomic DNA. Translation: AAC73388.1.
    AP009048 Genomic DNA. Translation: BAE76069.1.
    PIRiE64754.
    RefSeqiNP_414819.1. NC_000913.3.
    YP_488581.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73388; AAC73388; b0285.
    BAE76069; BAE76069; BAE76069.
    GeneIDi12930791.
    945710.
    KEGGiecj:Y75_p0277.
    eco:b0285.
    PATRICi32115693. VBIEscCol129921_0289.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73857 Genomic DNA. Translation: AAB18014.1 .
    U00096 Genomic DNA. Translation: AAC73388.1 .
    AP009048 Genomic DNA. Translation: BAE76069.1 .
    PIRi E64754.
    RefSeqi NP_414819.1. NC_000913.3.
    YP_488581.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P77324.
    SMRi P77324. Positions 3-271.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P77324. 7 interactions.
    STRINGi 511145.b0285.

    Proteomic databases

    PRIDEi P77324.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73388 ; AAC73388 ; b0285 .
    BAE76069 ; BAE76069 ; BAE76069 .
    GeneIDi 12930791.
    945710.
    KEGGi ecj:Y75_p0277.
    eco:b0285.
    PATRICi 32115693. VBIEscCol129921_0289.

    Organism-specific databases

    EchoBASEi EB3328.
    EcoGenei EG13558. yagS.

    Phylogenomic databases

    eggNOGi COG1319.
    HOGENOMi HOG000244727.
    KOi K11178.
    OMAi PCNKRRP.
    OrthoDBi EOG66TG3V.
    PhylomeDBi P77324.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6156-MONOMER.
    ECOL316407:JW0279-MONOMER.
    MetaCyc:G6156-MONOMER.

    Miscellaneous databases

    PROi P77324.

    Gene expression databases

    Genevestigatori P77324.

    Family and domain databases

    Gene3Di 3.30.43.10. 1 hit.
    3.30.465.10. 2 hits.
    InterProi IPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    [Graphical view ]
    Pfami PF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    [Graphical view ]
    SMARTi SM01092. CO_deh_flav_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55447. SSF55447. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEi PS51387. FAD_PCMH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiYAGS_ECOLI
    AccessioniPrimary (citable) accession number: P77324
    Secondary accession number(s): Q2MCD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3