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Protein

Putative xanthine dehydrogenase YagS FAD-binding subunit

Gene

yagS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei212FAD; via amide nitrogen and carbonyl oxygenSequence analysis1
Binding sitei227FADSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33FADSequence analysis8
Nucleotide bindingi107 – 111FADSequence analysis5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism, Purine salvage
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G6156-MONOMER.
MetaCyc:G6156-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative xanthine dehydrogenase YagS FAD-binding subunit (EC:1.17.1.4)
Gene namesi
Name:yagS
Ordered Locus Names:b0285, JW0279
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13558. yagS.

Subcellular locationi

GO - Cellular componenti

  • periplasmic space Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660941 – 318Putative xanthine dehydrogenase YagS FAD-binding subunitAdd BLAST318

Proteomic databases

PaxDbiP77324.
PRIDEiP77324.

Interactioni

Subunit structurei

Heterotrimer of YagR, YagS and YagT.Curated

Protein-protein interaction databases

BioGridi4259785. 121 interactors.
IntActiP77324. 7 interactors.
STRINGi316385.ECDH10B_0273.

Structurei

Secondary structure

1318
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Helixi12 – 21Combined sources10
Beta strandi25 – 30Combined sources6
Helixi34 – 39Combined sources6
Beta strandi46 – 50Combined sources5
Beta strandi59 – 61Combined sources3
Beta strandi65 – 70Combined sources6
Helixi75 – 80Combined sources6
Helixi82 – 87Combined sources6
Helixi89 – 96Combined sources8
Helixi101 – 106Combined sources6
Helixi109 – 113Combined sources5
Helixi120 – 123Combined sources4
Turni130 – 132Combined sources3
Turni140 – 142Combined sources3
Beta strandi150 – 152Combined sources3
Helixi165 – 171Combined sources7
Beta strandi175 – 179Combined sources5
Beta strandi185 – 189Combined sources5
Helixi190 – 192Combined sources3
Beta strandi212 – 218Combined sources7
Beta strandi224 – 231Combined sources8
Beta strandi233 – 238Combined sources6
Beta strandi241 – 248Combined sources8
Beta strandi254 – 264Combined sources11
Helixi269 – 272Combined sources4
Helixi273 – 277Combined sources5
Helixi279 – 286Combined sources8
Turni287 – 289Combined sources3
Turni294 – 297Combined sources4
Helixi298 – 315Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5G5GX-ray1.70B1-318[»]
5G5HX-ray2.30B1-318[»]
ProteinModelPortaliP77324.
SMRiP77324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 223FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST223

Phylogenomic databases

eggNOGiENOG4105DIP. Bacteria.
COG1319. LUCA.
HOGENOMiHOG000244727.
InParanoidiP77324.
KOiK11178.
PhylomeDBiP77324.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiView protein in InterPro
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
PfamiView protein in Pfam
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
SMARTiView protein in SMART
SM01092. CO_deh_flav_C. 1 hit.
SUPFAMiSSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiView protein in PROSITE
PS51387. FAD_PCMH. 1 hit.

Sequencei

Sequence statusi: Complete.

P77324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAFTYERVN TPAEAALSAQ RVPGAKFIAG GTNLLDLMKL EIETPTHLID
60 70 80 90 100
VNGLGLDKIE VTDAGGLRIG ALVRNTDLAA HERVRRDYAV LSRALLAGAS
110 120 130 140 150
GQLRNQATTA GNLLQRTRCP YFYDTNQPCN KRLPGSGCAA LEGFSRQHAV
160 170 180 190 200
VGVSEACIAT HPSDMAVAMR LLDAVVETIT PEGKTRSITL ADFYHPPGKT
210 220 230 240 250
PHIETALLPG ELIVAVTLPP PLGGKHIYRK VRDRASYAFA LVSVAAIIQP
260 270 280 290 300
DGSGRVALGG VAHKPWRIEA ADAQLSQGAQ AVYDTLFASA HPTAENTFKL
310
LLAKRTLASV LAEARAQA
Length:318
Mass (Da):33,858
Last modified:February 1, 1997 - v1
Checksum:i7D51C2B30C9BB222
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18014.1.
U00096 Genomic DNA. Translation: AAC73388.1.
AP009048 Genomic DNA. Translation: BAE76069.1.
PIRiE64754.
RefSeqiNP_414819.1. NC_000913.3.
WP_000643333.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73388; AAC73388; b0285.
BAE76069; BAE76069; BAE76069.
GeneIDi945710.
KEGGiecj:JW0279.
eco:b0285.
PATRICifig|1411691.4.peg.1993.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiYAGS_ECOLI
AccessioniPrimary (citable) accession number: P77324
Secondary accession number(s): Q2MCD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 5, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references