Reviewed, UniProtKB/Swiss-Prot P77293 (GTRB_ECOLI)

Names and origin

Protein names

Recommended name:
    Bactoprenol glucosyl transferase homolog from prophage CPS-53
    EC=2.4.1.-

Gene names

Name:	yfdH
Ordered Locus Names:	b2351, JW2347

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in O antigen modification. Catalyzes the transfer of the glucose residue from UDP-glucose to a lipid carrier By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GtrB subfamily.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fadJ	P77399	1	EBI-545379,EBI-545361

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Bactoprenol glucosyl transferase homolog from prophage CPS-53		PRO_0000059194
Regions
	Topological domain	1 – 227	227	Cytoplasmic Potential
	Transmembrane	228 – 248	21	Potential
	Topological domain	249 – 262	14	Periplasmic Potential
	Transmembrane	263 – 283	21	Potential
	Topological domain	284 – 306	23	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P77293-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 600411D3D201B4E6	FASTA	306	34,635
	10 20 30 40 50 60 MKISLVVPVF NEEEAIPIFY KTVREFEELK SYEVEIVFIN DGSKDATESI INALAVSDPL 70 80 90 100 110 120 VVPLSFTRNF GKEPALFAGL DHATGDAIIP IDVDLQDPIE VIPHLIEKWQ AGADMVLAKR 130 140 150 160 170 180 SDRSTDGRLK RKTAEWFYKL HNKISNPKIE ENVGDFRLMS RDVVENIKLM PERNLFMKGI 190 200 210 220 230 240 LSWVGGKTDI VEYVRAERIA GDTKFNGWKL WNLALEGITS FSTFPLRIWT YIGLVVASVA 250 260 270 280 290 300 FIYGAWMILD TIIFGNAVRG YPSLLVSILF LGGIQMIGIG VLGEYIGRTY IETKKRPKYI IKRVKK

References

[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00096 Genomic DNA. Translation: AAC75410.1. AP009048 Genomic DNA. Translation: BAA16210.1.
PIR	D65008.
RefSeq	AP_002950.1. NP_416852.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:11997N.
IntAct	P77293. 1 interaction.
STRING	P77293.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	949098.
GenomeReviews	Gene locus JW2347 in contig AP009048_GR. Gene locus b2351 in contig U00096_GR.
KEGG	ecj:JW2347. eco:b2351.
Organism-specific databases
EchoBASE	EB3884.
EcoGene	EG14132. yfdH.
CMR	Search...
Phylogenomic databases
HOGENOM	P77293.
OMA	IYYRVHN
Enzyme and pathway databases
BioCyc	EcoCyc:G7220-MON. ECOL168927:B2351-MON.
Gene expression databases
Genevestigator	P77293.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GTRB_ECOLI

Accession

Primary (citable) accession number: P77293

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	November 24, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)