P77293 (GTRB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bactoprenol glucosyl transferase homolog from prophage CPS-53
EC=2.4.1.-

Gene names

Name:	yfdH
Ordered Locus Names:	b2351, JW2347

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in O antigen modification. Catalyzes the transfer of the glucose residue from UDP-glucose to a lipid carrier By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GtrB subfamily.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Bactoprenol glucosyl transferase homolog from prophage CPS-53		PRO_0000059194
Regions
	Topological domain	1 – 227	227	Cytoplasmic Potential
	Transmembrane	228 – 248	21	Helical; Potential
	Topological domain	249 – 262	14	Periplasmic Potential
	Transmembrane	263 – 283	21	Helical; Potential
	Topological domain	284 – 306	23	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P77293 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 600411D3D201B4E6	FASTA	306	34,635
	10 20 30 40 50 60 MKISLVVPVF NEEEAIPIFY KTVREFEELK SYEVEIVFIN DGSKDATESI INALAVSDPL 70 80 90 100 110 120 VVPLSFTRNF GKEPALFAGL DHATGDAIIP IDVDLQDPIE VIPHLIEKWQ AGADMVLAKR 130 140 150 160 170 180 SDRSTDGRLK RKTAEWFYKL HNKISNPKIE ENVGDFRLMS RDVVENIKLM PERNLFMKGI 190 200 210 220 230 240 LSWVGGKTDI VEYVRAERIA GDTKFNGWKL WNLALEGITS FSTFPLRIWT YIGLVVASVA 250 260 270 280 290 300 FIYGAWMILD TIIFGNAVRG YPSLLVSILF LGGIQMIGIG VLGEYIGRTY IETKKRPKYI IKRVKK

References

[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC75410.1. AP009048 Genomic DNA. Translation: BAA16210.1.
PIR	D65008.
RefSeq	NP_416852.1. NC_000913.2. YP_490592.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P77293.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11997N.
IntAct	P77293. 1 interaction.
STRING	511145.b2351.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75410; AAC75410; b2351. BAA16210; BAA16210; BAA16210.
GeneID	12931548. 949098.
KEGG	ecj:Y75_p2316. eco:b2351.
PATRIC	32120075. VBIEscCol129921_2446.
Organism-specific databases
EchoBASE	EB3884.
EcoGene	EG14132. yfdH.
Phylogenomic databases
eggNOG	COG0463.
HOGENOM	HOG000283247.
OMA	GKESAMI.
ProtClustDB	CLSK891776.
Enzyme and pathway databases
BioCyc	EcoCyc:G7220-MONOMER. ECOL316407:JW2347-MONOMER.
Gene expression databases
Genevestigator	P77293.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GTRB_ECOLI

Accession

Primary (citable) accession number: P77293

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program