Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Multidrug resistance-like ATP-binding protein MdlA

Gene

mdlA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi369 – 3768ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:MDLA-MONOMER.
ECOL316407:JW0438-MONOMER.

Protein family/group databases

TCDBi3.A.1.106.13. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance-like ATP-binding protein MdlA (EC:3.6.3.44)
Gene namesi
Name:mdlA
Synonyms:mdl
Ordered Locus Names:b0448, JW0438
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11622. mdlA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei53 – 7321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei134 – 15421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei155 – 17521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei248 – 26821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei280 – 30021HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590Multidrug resistance-like ATP-binding protein MdlAPRO_0000092492Add
BLAST

Proteomic databases

PaxDbiP77265.

Interactioni

Protein-protein interaction databases

BioGridi4261677. 138 interactions.
DIPiDIP-10173N.
IntActiP77265. 1 interaction.
STRINGi511145.b0448.

Structurei

3D structure databases

ProteinModelPortaliP77265.
SMRiP77265. Positions 17-577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 303286ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini337 – 570234ABC transporterPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
InParanoidiP77265.
KOiK18889.
OMAiVTAQHFT.
OrthoDBiEOG6T7N3V.
PhylomeDBiP77265.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77265-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLFAQLSWY FRREWRRYLG AVALLVIIAM LQLVPPKVVG IVVDGVTEQH
60 70 80 90 100
FTTGQILMWI ATMVLIAVVV YLLRYVWRVL LFGASYQLAV ELREDYYRQL
110 120 130 140 150
SRQHPEFYLR HRTGDLMARA TNDVDRVVFA AGEGVLTLVD SLVMGCAVLI
160 170 180 190 200
MMSTQISWQL TLFSLLPMPV MAIMIKRNGD ALHERFKLAQ AAFSSLNDRT
210 220 230 240 250
QESLTSIRMI KAFGLEDRQS ALFAADAEDT GKKNMRVARI DARFDPTIYI
260 270 280 290 300
AIGMANLLAI GGGSWMVVQG SLTLGQLTSF MMYLGLMIWP MLALAWMFNI
310 320 330 340 350
VERGSAAYSR IRAMLAEAPV VNDGSEPVPE GRGELDVNIH QFTYPQTDHP
360 370 380 390 400
ALENVNFALK PGQMLGICGP TGSGKSTLLS LIQRHFDVSE GDIRFHDIPL
410 420 430 440 450
TKLQLDSWRS RLAVVSQTPF LFSDTVANNI ALGCPNATQQ EIEHVARLAS
460 470 480 490 500
VHDDILRLPQ GYDTEVGERG VMLSGGQKQR ISIARALLVN AEILILDDAL
510 520 530 540 550
SAVDGRTEHQ ILHNLRQWGQ GRTVIISAHR LSALTEASEI IVMQHGHIAQ
560 570 580 590
RGNHDVLAQQ SGWYRDMYRY QQLEAALDDA PENREEAVDA
Length:590
Mass (Da):66,017
Last modified:February 1, 1997 - v1
Checksum:iAD1CEBE41030200D
GO

Sequence cautioni

The sequence AAC36870.1 differs from that shown. Reason: Frameshift at position 546. Fuses together mdlA and mdlB.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1451G → A in AAC36870 (PubMed:7904973).Curated
Sequence conflicti234 – 2352NM → KL in AAC36870 (PubMed:7904973).Curated
Sequence conflicti318 – 3181A → R in AAC36870 (PubMed:7904973).Curated
Sequence conflicti371 – 3711T → N in AAC36870 (PubMed:7904973).Curated
Sequence conflicti390 – 3901E → P in AAC36870 (PubMed:7904973).Curated
Sequence conflicti410 – 4112SR → TG in AAC36870 (PubMed:7904973).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08627 Unassigned DNA. Translation: AAC36870.1. Frameshift.
U82664 Genomic DNA. Translation: AAB40204.1.
U00096 Genomic DNA. Translation: AAC73551.1.
AP009048 Genomic DNA. Translation: BAE76228.1.
PIRiH64774.
RefSeqiNP_414982.1. NC_000913.3.
WP_001235649.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73551; AAC73551; b0448.
BAE76228; BAE76228; BAE76228.
GeneIDi945092.
KEGGiecj:JW0438.
eco:b0448.
PATRICi32116049. VBIEscCol129921_0466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08627 Unassigned DNA. Translation: AAC36870.1. Frameshift.
U82664 Genomic DNA. Translation: AAB40204.1.
U00096 Genomic DNA. Translation: AAC73551.1.
AP009048 Genomic DNA. Translation: BAE76228.1.
PIRiH64774.
RefSeqiNP_414982.1. NC_000913.3.
WP_001235649.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77265.
SMRiP77265. Positions 17-577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261677. 138 interactions.
DIPiDIP-10173N.
IntActiP77265. 1 interaction.
STRINGi511145.b0448.

Protein family/group databases

TCDBi3.A.1.106.13. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP77265.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73551; AAC73551; b0448.
BAE76228; BAE76228; BAE76228.
GeneIDi945092.
KEGGiecj:JW0438.
eco:b0448.
PATRICi32116049. VBIEscCol129921_0466.

Organism-specific databases

EchoBASEiEB1579.
EcoGeneiEG11622. mdlA.

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
InParanoidiP77265.
KOiK18889.
OMAiVTAQHFT.
OrthoDBiEOG6T7N3V.
PhylomeDBiP77265.

Enzyme and pathway databases

BioCyciEcoCyc:MDLA-MONOMER.
ECOL316407:JW0438-MONOMER.

Miscellaneous databases

PROiP77265.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance."
    Allikmets R., Gerrard B.C., Court D., Dean M.C.
    Gene 136:231-236(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: TAP90 / ATCC 47037.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMDLA_ECOLI
AccessioniPrimary (citable) accession number: P77265
Secondary accession number(s): P30751, Q2MBX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: February 17, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.