P77265 (MDLA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multidrug resistance-like ATP-binding protein MdlA
EC=3.6.3.44

Gene names

Name:	mdlA
Synonyms:	mdl
Ordered Locus Names:	b0448, JW0438

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Belongs to the ABC transporter superfamily. Drug exporter-2 (TC 3.A.1.117) family. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.
Sequence caution	The sequence AAC36870.1 differs from that shown. Reason: Frameshift at position 546. Fuses together mdlA and mdlB.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Multidrug resistance-like ATP-binding protein MdlA		PRO_0000092492
Regions
	Transmembrane	23 – 43	21	Helical; Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	134 – 154	21	Helical; Potential
	Transmembrane	155 – 175	21	Helical; Potential
	Transmembrane	248 – 268	21	Helical; Potential
	Transmembrane	280 – 300	21	Helical; Potential
	Domain	18 – 303	286	ABC transmembrane type-1
	Domain	337 – 570	234	ABC transporter
	Nucleotide binding	369 – 376	8	ATP Potential
Experimental info
	Sequence conflict	145	1	G → A in AAC36870. Ref.1
	Sequence conflict	234 – 235	2	NM → KL in AAC36870. Ref.1
	Sequence conflict	318	1	A → R in AAC36870. Ref.1
	Sequence conflict	371	1	T → N in AAC36870. Ref.1
	Sequence conflict	390	1	E → P in AAC36870. Ref.1
	Sequence conflict	410 – 411	2	SR → TG in AAC36870. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P77265 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: AD1CEBE41030200D	FASTA	590	66,017
	10 20 30 40 50 60 MRLFAQLSWY FRREWRRYLG AVALLVIIAM LQLVPPKVVG IVVDGVTEQH FTTGQILMWI 70 80 90 100 110 120 ATMVLIAVVV YLLRYVWRVL LFGASYQLAV ELREDYYRQL SRQHPEFYLR HRTGDLMARA 130 140 150 160 170 180 TNDVDRVVFA AGEGVLTLVD SLVMGCAVLI MMSTQISWQL TLFSLLPMPV MAIMIKRNGD 190 200 210 220 230 240 ALHERFKLAQ AAFSSLNDRT QESLTSIRMI KAFGLEDRQS ALFAADAEDT GKKNMRVARI 250 260 270 280 290 300 DARFDPTIYI AIGMANLLAI GGGSWMVVQG SLTLGQLTSF MMYLGLMIWP MLALAWMFNI 310 320 330 340 350 360 VERGSAAYSR IRAMLAEAPV VNDGSEPVPE GRGELDVNIH QFTYPQTDHP ALENVNFALK 370 380 390 400 410 420 PGQMLGICGP TGSGKSTLLS LIQRHFDVSE GDIRFHDIPL TKLQLDSWRS RLAVVSQTPF 430 440 450 460 470 480 LFSDTVANNI ALGCPNATQQ EIEHVARLAS VHDDILRLPQ GYDTEVGERG VMLSGGQKQR 490 500 510 520 530 540 ISIARALLVN AEILILDDAL SAVDGRTEHQ ILHNLRQWGQ GRTVIISAHR LSALTEASEI 550 560 570 580 590 IVMQHGHIAQ RGNHDVLAQQ SGWYRDMYRY QQLEAALDDA PENREEAVDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance." Allikmets R., Gerrard B.C., Court D., Dean M.C. Gene 136:231-236(1993) [PubMed: 7904973] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TAP90 / ATCC 47037.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08627 Unassigned DNA. Translation: AAC36870.1. Frameshift. U82664 Genomic DNA. Translation: AAB40204.1. U00096 Genomic DNA. Translation: AAC73551.1. AP009048 Genomic DNA. Translation: BAE76228.1.
PIR	H64774.
RefSeq	NP_414982.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P77265.
SMR	P77265. Positions 3-574.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10173N.
IntAct	P77265. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002306; EBESCP00000002306; EBESCG00000001881. EBESCT00000015992; EBESCP00000015283; EBESCG00000015052.
GeneID	945092.
GenomeReviews	Gene locus JW0438 in contig AP009048_GR. Gene locus b0448 in contig U00096_GR.
KEGG	ecj:JW0438. eco:b0448.
PATRIC	32116049. VBIEscCol129921_0466.
Organism-specific databases
EchoBASE	EB1579.
EcoGene	EG11622. mdlA.
Phylogenomic databases
eggNOG	COG1132.
GeneTree	EBGT00050000008240.
HOGENOM	HBG758042.
OMA	PVVNDGT.
PhylomeDB	P77265.
ProtClustDB	PRK10789.
Enzyme and pathway databases
BioCyc	EcoCyc:MDLA-MONOMER.
Gene expression databases
Genevestigator	P77265.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K06147.
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 1 hit.
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDLA_ECOLI

Accession

Primary (citable) accession number: P77265
Secondary accession number(s): P30751, Q2MBX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program