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Protein

N-ethylmaleimide reductase

Gene

nemA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reduction of N-ethylmaleimide (NEM) to N-ethylsuccinimide.1 Publication

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101FMNBy similarity1
Active sitei187Proton donorBy similarity1
Binding sitei234FMNBy similarity1
Binding sitei325FMNBy similarity1

GO - Molecular functioni

  • chromate reductase activity Source: EcoCyc
  • FMN binding Source: EcoCyc
  • N-ethylmaleimide reductase activity Source: EcoCyc
  • oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: EcoCyc

GO - Biological processi

  • 2,4,6-trinitrotoluene catabolic process Source: EcoCyc
  • nitroglycerin metabolic process Source: EcoCyc
  • xenobiotic metabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
LigandFlavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6890-MONOMER.
MetaCyc:G6890-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-ethylmaleimide reductase (EC:1.-.-.-)
Alternative name(s):
N-ethylmaleimide reducing enzyme
Gene namesi
Name:nemA
Synonyms:ydhN
Ordered Locus Names:b1650, JW1642
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13546. nemA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001944811 – 365N-ethylmaleimide reductaseAdd BLAST365

Proteomic databases

PaxDbiP77258.
PRIDEiP77258.

2D gel databases

SWISS-2DPAGEiP77258.

Expressioni

Inductioni

Repressed by NemR. Induced by N-ethylmaleimide, probably via inactivation of NemR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259388. 16 interactors.
IntActiP77258. 3 interactors.
STRINGi316385.ECDH10B_1784.

Structurei

3D structure databases

ProteinModelPortaliP77258.
SMRiP77258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 185Substrate-bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CCY. Bacteria.
COG1902. LUCA.
HOGENOMiHOG000116231.
InParanoidiP77258.
KOiK10680.
PhylomeDBiP77258.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
PfamiView protein in Pfam
PF00724. Oxidored_FMN. 1 hit.

Sequencei

Sequence statusi: Complete.

P77258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEKLYSPL KVGAITAANR IFMAPLTRLR SIEPGDIPTP LMAEYYRQRA
60 70 80 90 100
SAGLIISEAT QISAQAKGYA GAPGIHSPEQ IAAWKKITAG VHAENGHMAV
110 120 130 140 150
QLWHTGRISH ASLQPGGQAP VAPSALSAGT RTSLRDENGQ AIRVETSMPR
160 170 180 190 200
ALELEEIPGI VNDFRQAIAN AREAGFDLVE LHSAHGYLLH QFLSPSSNHR
210 220 230 240 250
TDQYGGSVEN RARLVLEVVD AGIEEWGADR IGIRVSPIGT FQNTDNGPNE
260 270 280 290 300
EADALYLIEQ LGKRGIAYLH MSEPDWAGGE PYTDAFREKV RARFHGPIIG
310 320 330 340 350
AGAYTVEKAE TLIGKGLIDA VAFGRDWIAN PDLVARLQRK AELNPQRAES
360
FYGGGAEGYT DYPTL
Length:365
Mass (Da):39,516
Last modified:February 1, 1997 - v1
Checksum:iEFCFE04B42CE9C7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86931 Genomic DNA. Translation: BAA13186.1.
U00096 Genomic DNA. Translation: AAC74722.1.
AP009048 Genomic DNA. Translation: BAE76493.1.
PIRiJC5605.
RefSeqiNP_416167.1. NC_000913.3.
WP_000093589.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74722; AAC74722; b1650.
BAE76493; BAE76493; BAE76493.
GeneIDi946164.
KEGGiecj:JW1642.
eco:b1650.
PATRICifig|1411691.4.peg.609.

Similar proteinsi

Entry informationi

Entry nameiNEMA_ECOLI
AccessioniPrimary (citable) accession number: P77258
Secondary accession number(s): Q2MB63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: August 30, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families