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Protein

N-ethylmaleimide reductase

Gene

nemA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reduction of N-ethylmaleimide (NEM) to N-ethylsuccinimide.1 Publication

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011FMNBy similarity
Active sitei187 – 1871Proton donorBy similarity
Binding sitei234 – 2341FMNBy similarity
Binding sitei325 – 3251FMNBy similarity

GO - Molecular functioni

  • chromate reductase activity Source: EcoCyc
  • FMN binding Source: EcoCyc
  • NADPH dehydrogenase activity Source: GO_Central
  • N-ethylmaleimide reductase activity Source: EcoCyc
  • oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: EcoCyc

GO - Biological processi

  • 2,4,6-trinitrotoluene catabolic process Source: EcoCyc
  • nitroglycerin metabolic process Source: EcoCyc
  • xenobiotic metabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6890-MONOMER.
ECOL316407:JW1642-MONOMER.
MetaCyc:G6890-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-ethylmaleimide reductase (EC:1.-.-.-)
Alternative name(s):
N-ethylmaleimide reducing enzyme
Gene namesi
Name:nemA
Synonyms:ydhN
Ordered Locus Names:b1650, JW1642
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13546. nemA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365N-ethylmaleimide reductasePRO_0000194481Add
BLAST

Proteomic databases

EPDiP77258.
PaxDbiP77258.
PRIDEiP77258.

2D gel databases

SWISS-2DPAGEP77258.

Expressioni

Inductioni

Repressed by NemR. Induced by N-ethylmaleimide, probably via inactivation of NemR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259388. 16 interactions.
IntActiP77258. 3 interactions.
STRINGi511145.b1650.

Structurei

3D structure databases

ProteinModelPortaliP77258.
SMRiP77258. Positions 4-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni182 – 1854Substrate-bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CCY. Bacteria.
COG1902. LUCA.
HOGENOMiHOG000116231.
InParanoidiP77258.
KOiK10680.
OMAiRFRQNAP.
PhylomeDBiP77258.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEKLYSPL KVGAITAANR IFMAPLTRLR SIEPGDIPTP LMAEYYRQRA
60 70 80 90 100
SAGLIISEAT QISAQAKGYA GAPGIHSPEQ IAAWKKITAG VHAENGHMAV
110 120 130 140 150
QLWHTGRISH ASLQPGGQAP VAPSALSAGT RTSLRDENGQ AIRVETSMPR
160 170 180 190 200
ALELEEIPGI VNDFRQAIAN AREAGFDLVE LHSAHGYLLH QFLSPSSNHR
210 220 230 240 250
TDQYGGSVEN RARLVLEVVD AGIEEWGADR IGIRVSPIGT FQNTDNGPNE
260 270 280 290 300
EADALYLIEQ LGKRGIAYLH MSEPDWAGGE PYTDAFREKV RARFHGPIIG
310 320 330 340 350
AGAYTVEKAE TLIGKGLIDA VAFGRDWIAN PDLVARLQRK AELNPQRAES
360
FYGGGAEGYT DYPTL
Length:365
Mass (Da):39,516
Last modified:February 1, 1997 - v1
Checksum:iEFCFE04B42CE9C7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86931 Genomic DNA. Translation: BAA13186.1.
U00096 Genomic DNA. Translation: AAC74722.1.
AP009048 Genomic DNA. Translation: BAE76493.1.
PIRiJC5605.
RefSeqiNP_416167.1. NC_000913.3.
WP_000093589.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74722; AAC74722; b1650.
BAE76493; BAE76493; BAE76493.
GeneIDi946164.
KEGGiecj:JW1642.
eco:b1650.
PATRICi32118602. VBIEscCol129921_1721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86931 Genomic DNA. Translation: BAA13186.1.
U00096 Genomic DNA. Translation: AAC74722.1.
AP009048 Genomic DNA. Translation: BAE76493.1.
PIRiJC5605.
RefSeqiNP_416167.1. NC_000913.3.
WP_000093589.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77258.
SMRiP77258. Positions 4-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259388. 16 interactions.
IntActiP77258. 3 interactions.
STRINGi511145.b1650.

2D gel databases

SWISS-2DPAGEP77258.

Proteomic databases

EPDiP77258.
PaxDbiP77258.
PRIDEiP77258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74722; AAC74722; b1650.
BAE76493; BAE76493; BAE76493.
GeneIDi946164.
KEGGiecj:JW1642.
eco:b1650.
PATRICi32118602. VBIEscCol129921_1721.

Organism-specific databases

EchoBASEiEB3316.
EcoGeneiEG13546. nemA.

Phylogenomic databases

eggNOGiENOG4105CCY. Bacteria.
COG1902. LUCA.
HOGENOMiHOG000116231.
InParanoidiP77258.
KOiK10680.
OMAiRFRQNAP.
PhylomeDBiP77258.

Enzyme and pathway databases

BioCyciEcoCyc:G6890-MONOMER.
ECOL316407:JW1642-MONOMER.
MetaCyc:G6890-MONOMER.

Miscellaneous databases

PROiP77258.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEMA_ECOLI
AccessioniPrimary (citable) accession number: P77258
Secondary accession number(s): Q2MB63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.