P77257 (LSRA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autoinducer 2 import ATP-binding protein LsrA
Short name=AI-2 import ATP-binding protein LsrA
EC=3.6.3.-

Alternative name(s):
EGO10A

Gene names

Name:	lsrA
Synonyms:	ego, ydeX
Ordered Locus Names:	b1513, JW1506

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Responsible for energy coupling to the transport system Probable. This protein is essential for aerobic growth. Ref.5
Subunit structure	The complex is composed of two ATP-binding proteins (LsrA), two transmembrane proteins (LsrC and LsrD) and a solute-binding protein (LsrB) Probable.
Subcellular location	Cell inner membrane; Peripheral membrane protein Potential.
Induction	In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter. Ref.5 Ref.6
Sequence similarities	Belongs to the ABC transporter superfamily. AI-2 autoinducer porter (TC 3.A.1.2.8) family. [View classification] Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Autoinducer 2 import ATP-binding protein LsrA		PRO_0000092321
Regions
	Domain	12 – 240	229	ABC transporter 1
	Domain	240 – 503	264	ABC transporter 2
	Nucleotide binding	44 – 51	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P77257 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: CC399008BFFD55FA	FASTA	511	55,821
	10 20 30 40 50 60 MQTSDTRALP LLCARSVYKQ YSGVNVLKGI DFTLHQGEVH ALLGGNGAGK STLMKIIAGI 70 80 90 100 110 120 TPADSGTLEI EGNNYVRLTP VHAHQLGIYL VPQEPLLFPS LSIKENILFG LAKKQLSMQK 130 140 150 160 170 180 MKNLLAALGC QFDLHSLAGS LDVADRQMVE ILRGLMRDSR ILILDEPTAS LTPAETERLF 190 200 210 220 230 240 SRLQELLATG VGIVFISHKL PEIRQIADRI SVMRDGTIAL SGKTSELSTD DIIQAITPAV 250 260 270 280 290 300 REKSLSASQK LWLELPGNRP QHAAGTPVLT LENLTGEGFR NVSLTLNAGE ILGLAGLVGA 310 320 330 340 350 360 GRTELAETLY GLRTLRGGRI MLNGKEINKL STGERLLRGL VYLPEDRQSS GLNLDASLAW 370 380 390 400 410 420 NVCALTHNLR GFWAKTAKDN ATLERYRRAL NIKFNQPEQA ARTLSGGNQQ KILIAKCLEA 430 440 450 460 470 480 SPQVLIVDEP TRGVDVSARN DIYQLLRSIA AQNVAVLLIS SDLEEIELMA DRVYVMHQGE 490 500 510 ITHSALTERD INVETIMRVA FGDSQRQEAS C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kwon H.B., Lee S.H., Choe M.H. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli." Xavier K.B., Bassler B.L. J. Bacteriol. 187:238-248(2005) [PubMed: 15601708] [Abstract] Cited for: FUNCTION IN AI-2 IMPORT, INDUCTION. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli." Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E. J. Bacteriol. 187:2066-2076(2005) [PubMed: 15743955] [Abstract] Cited for: INDUCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF089855 Genomic DNA. Translation: AAC61747.1. U00096 Genomic DNA. Translation: AAC74586.1. AP009048 Genomic DNA. Translation: BAA15200.1.
PIR	D64905.
RefSeq	NP_416030.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P77257.
SMR	P77257. Positions 11-503.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77257. 2 interactions.
Protein family/group databases
TCDB	3.A.1.2.8. ATP-binding cassette (ABC) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001115; EBESCP00000001115; EBESCG00000000926. EBESCT00000016258; EBESCP00000015549; EBESCG00000015318.
GeneID	945680.
GenomeReviews	Gene locus JW1506 in contig AP009048_GR. Gene locus b1513 in contig U00096_GR.
KEGG	ecj:JW1506. eco:b1513.
PATRIC	32118322. VBIEscCol129921_1581.
Organism-specific databases
EchoBASE	EB3567.
EcoGene	EG13806. lsrA.
Phylogenomic databases
eggNOG	COG1129.
GeneTree	EBGT00050000008244.
HOGENOM	HBG758042.
OMA	IAHSALT.
PhylomeDB	P77257.
ProtClustDB	PRK15439.
Enzyme and pathway databases
BioCyc	EcoCyc:YDEX-MONOMER.
Gene expression databases
Genevestigator	P77257.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K10558.
Pfam	PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSRA_ECOLI

Accession

Primary (citable) accession number: P77257
Secondary accession number(s): P76879

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program