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Protein

Autoinducer 2 import ATP-binding protein LsrA

Gene

lsrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Responsible for energy coupling to the transport system (Probable). This protein is essential for aerobic growth.Curated1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 51ATPPROSITE-ProRule annotation8

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YDEX-MONOMER.
ECOL316407:JW1506-MONOMER.
MetaCyc:YDEX-MONOMER.

Protein family/group databases

TCDBi3.A.1.2.8. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Autoinducer 2 import ATP-binding protein LsrA (EC:3.6.3.-)
Short name:
AI-2 import ATP-binding protein LsrA
Alternative name(s):
EGO10A
Gene namesi
Name:lsrA
Synonyms:ego, ydeX
Ordered Locus Names:b1513, JW1506
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13806. lsrA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000923211 – 511Autoinducer 2 import ATP-binding protein LsrAAdd BLAST511

Proteomic databases

PaxDbiP77257.
PRIDEiP77257.

Expressioni

Inductioni

In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.2 Publications

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (LsrA), two transmembrane proteins (LsrC and LsrD) and a solute-binding protein (LsrB).Curated

Protein-protein interaction databases

BioGridi4260226. 15 interactors.
IntActiP77257. 3 interactors.
STRINGi511145.b1513.

Structurei

3D structure databases

ProteinModelPortaliP77257.
SMRiP77257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 240ABC transporter 1PROSITE-ProRule annotationAdd BLAST229
Domaini240 – 503ABC transporter 2PROSITE-ProRule annotationAdd BLAST264

Sequence similaritiesi

Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105C2J. Bacteria.
COG1129. LUCA.
InParanoidiP77257.
KOiK10558.
OMAiQEPMLFP.
PhylomeDBiP77257.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030281. LsrA.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24220:SF555. PTHR24220:SF555. 1 hit.
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTSDTRALP LLCARSVYKQ YSGVNVLKGI DFTLHQGEVH ALLGGNGAGK
60 70 80 90 100
STLMKIIAGI TPADSGTLEI EGNNYVRLTP VHAHQLGIYL VPQEPLLFPS
110 120 130 140 150
LSIKENILFG LAKKQLSMQK MKNLLAALGC QFDLHSLAGS LDVADRQMVE
160 170 180 190 200
ILRGLMRDSR ILILDEPTAS LTPAETERLF SRLQELLATG VGIVFISHKL
210 220 230 240 250
PEIRQIADRI SVMRDGTIAL SGKTSELSTD DIIQAITPAV REKSLSASQK
260 270 280 290 300
LWLELPGNRP QHAAGTPVLT LENLTGEGFR NVSLTLNAGE ILGLAGLVGA
310 320 330 340 350
GRTELAETLY GLRTLRGGRI MLNGKEINKL STGERLLRGL VYLPEDRQSS
360 370 380 390 400
GLNLDASLAW NVCALTHNLR GFWAKTAKDN ATLERYRRAL NIKFNQPEQA
410 420 430 440 450
ARTLSGGNQQ KILIAKCLEA SPQVLIVDEP TRGVDVSARN DIYQLLRSIA
460 470 480 490 500
AQNVAVLLIS SDLEEIELMA DRVYVMHQGE ITHSALTERD INVETIMRVA
510
FGDSQRQEAS C
Length:511
Mass (Da):55,821
Last modified:February 1, 1997 - v1
Checksum:iCC399008BFFD55FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF089855 Genomic DNA. Translation: AAC61747.1.
U00096 Genomic DNA. Translation: AAC74586.1.
AP009048 Genomic DNA. Translation: BAA15200.1.
PIRiD64905.
RefSeqiNP_416030.1. NC_000913.3.
WP_001194860.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74586; AAC74586; b1513.
BAA15200; BAA15200; BAA15200.
GeneIDi945680.
KEGGiecj:JW1506.
eco:b1513.
PATRICi32118322. VBIEscCol129921_1581.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF089855 Genomic DNA. Translation: AAC61747.1.
U00096 Genomic DNA. Translation: AAC74586.1.
AP009048 Genomic DNA. Translation: BAA15200.1.
PIRiD64905.
RefSeqiNP_416030.1. NC_000913.3.
WP_001194860.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77257.
SMRiP77257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260226. 15 interactors.
IntActiP77257. 3 interactors.
STRINGi511145.b1513.

Protein family/group databases

TCDBi3.A.1.2.8. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP77257.
PRIDEiP77257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74586; AAC74586; b1513.
BAA15200; BAA15200; BAA15200.
GeneIDi945680.
KEGGiecj:JW1506.
eco:b1513.
PATRICi32118322. VBIEscCol129921_1581.

Organism-specific databases

EchoBASEiEB3567.
EcoGeneiEG13806. lsrA.

Phylogenomic databases

eggNOGiENOG4105C2J. Bacteria.
COG1129. LUCA.
InParanoidiP77257.
KOiK10558.
OMAiQEPMLFP.
PhylomeDBiP77257.

Enzyme and pathway databases

BioCyciEcoCyc:YDEX-MONOMER.
ECOL316407:JW1506-MONOMER.
MetaCyc:YDEX-MONOMER.

Miscellaneous databases

PROiP77257.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030281. LsrA.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24220:SF555. PTHR24220:SF555. 1 hit.
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSRA_ECOLI
AccessioniPrimary (citable) accession number: P77257
Secondary accession number(s): P76879
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.