P77247 (YNIC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatase YniC
EC=3.1.3.-

Gene names

Name:	yniC
Ordered Locus Names:	b1727, JW1716

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates 2-deoxyglucose 6-phosphate and mannose 6-phosphate. Ref.4
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	2-deoxyglucose-6-phosphatase activity Inferred from direct assay. Source: EcoliWiki glucose-6-phosphatase activity Inferred from direct assay. Source: EcoliWiki metal ion binding Inferred from direct assay. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 222

222

Phosphatase YniC

PRO_0000108061

Sites

Active site

13

1

Nucleophile By similarity

Secondary structure

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222

Helix Strand Turn

Details...

Beta strand

9 – 12

4

Turn

15 – 17

3

Helix

22 – 35

14

Helix

40 – 45

6

Helix

54 – 64

11

Beta strand

68 – 70

3

Helix

72 – 90

19

Helix

97 – 106

10

Beta strand

110 – 117

8

Helix

119 – 128

10

Helix

132 – 134

3

Beta strand

136 – 140

5

Helix

152 – 161

10

Helix

165 – 167

3

Beta strand

168 – 174

7

Helix

175 – 183

9

Beta strand

187 – 190

4

Turn

194 – 198

5

Helix

200 – 204

5

Beta strand

205 – 208

4

Helix

212 – 214

3

Helix

217 – 221

5

Sequences

	Sequence		Length	Mass (Da)
	P77247 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 76FE1F2A331476A7	FASTA	222	24,330
	10 20 30 40 50 60 MSTPRQILAA IFDMDGLLID SEPLWDRAEL DVMASLGVDI SRRNELPDTL GLRIDMVVDL 70 80 90 100 110 120 WYARQPWNGP SRQEVVERVI ARAISLVEET RPLLPGVREA VALCKEQGLL VGLASASPLH 130 140 150 160 170 180 MLEKVLTMFD LRDSFDALAS AEKLPYSKPH PQVYLDCAAK LGVDPLTCVA LEDSVNGMIA 190 200 210 220 SKAARMRSIV VPAPEAQNDP RFVLADVKLS SLTELTAKDL LG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Enzyme genomics: application of general enzymatic screens to discover new enzymes." Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F. FEMS Microbiol. Rev. 29:263-279(2005) [PubMed: 15808744] [Abstract] Cited for: FUNCTION.
[5]	"Crystal structure of putative phosphatase YniC from Escherichia coli K12." Kim Y., Joachimiak A., Evdokimova E., Savchenko A., Edwards A.M. Submitted (AUG-2004) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC74797.1.
AP009048 Genomic DNA. Translation: BAA15508.1.

PIR

G64931.

RefSeq

NP_416241.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TE2	X-ray	1.76	A/B	1-222	[»]

ProteinModelPortal

P77247.

SMR

P77247. Positions 5-222.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-12777N.

IntAct

P77247. 8 interactions.

MINT

MINT-1257246.

2D gel databases

SWISS-2DPAGE

P77247.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001226; EBESCP00000001226; EBESCG00000001012.
EBESCT00000017557; EBESCP00000016848; EBESCG00000016613.

GeneID

945632.

GenomeReviews

Gene locus JW1716 in contig AP009048_GR.
Gene locus b1727 in contig U00096_GR.

KEGG

ecj:JW1716.
eco:b1727.

PATRIC

32118761. VBIEscCol129921_1798.

Organism-specific databases

EchoBASE

EB3744.

EcoGene

EG13988. yniC.

Phylogenomic databases

eggNOG

COG0637.

GeneTree

EBGT00050000009691.

HOGENOM

HBG742904.

OMA

WQRVEYE.

PhylomeDB

P77247.

ProtClustDB

PRK10826.

Enzyme and pathway databases

BioCyc

EcoCyc:G6932-MONOMER.
MetaCyc:G6932-MONOMER.

Gene expression databases

Genevestigator

P77247.

Family and domain databases

InterPro

IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
IPR006402. HAD-SF_hydro_IA_v3.
IPR005833. Haloacid_DH/epoxide_hydro.
IPR023198. PGP_dom2.
[Graphical view]

Gene3D

G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
G3DSA:1.10.150.240. PGP_dom2. 1 hit.

KO

K01112.

Pfam

PF00702. Hydrolase. 1 hit.
[Graphical view]

PRINTS

PR00413. HADHALOGNASE.

SUPFAM

SSF56784. HAD-like_dom. 1 hit.

TIGRFAMs

TIGR01509. HAD-SF-IA-v3. 1 hit.

ProtoNet

Search...

Entry information

Entry name

YNIC_ECOLI

Accession

Primary (citable) accession number: P77247
Secondary accession number(s): P78167

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program