P77245 (MURR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator MurR

Alternative name(s):
MurPQ operon repressor

Gene names

Name:	murR
Synonyms:	yfeT
Ordered Locus Names:	b2427, JW2420

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses the expression of the murPQ operon involved in the uptake and degradation of N-acetylmuramic acid (MurNAc). Binds to two adjacent inverted repeats within the operator region. MurNAc 6-phosphate, the substrate of MurQ, is the specific inducer that weakens binding of MurR to the operator. Also represses its own transcription. Ref.4
Pathway	Amino-sugar metabolism; N-acetylmuramate degradation [regulation]. Ref.4
Subunit structure	Homotetramer Probable. Ref.4
Induction	Repressed by itself and by the cAMP receptor protein crp. Ref.4
Disruption phenotype	Cells lacking this gene show an abolishment of the extensive lag phase observed when grown on MurNAc and a 20-fold enhancement of murQ transcription. Ref.4
Miscellaneous	Neither GlcNAc-6-P, GlcNAc, anhydroMurNAc, MurNAc, nor muramyl dipeptide have an effect on MurR binding to the operator site. HAMAP-Rule MF_02108
Sequence similarities	Contains 1 HTH rpiR-type DNA-binding domain. Contains 1 SIS domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-acetylmuramic acid catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway carbohydrate metabolic process Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.4. Source: EcoCyc regulation of carbohydrate catabolic process Inferred from electronic annotation. Source: HAMAP transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP carbohydrate binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	HTH-type transcriptional regulator MurR HAMAP-Rule MF_02108		PRO_0000068625
Regions
	Domain	1 – 77	77	HTH rpiR-type
	Domain	128 – 268	141	SIS
	DNA binding	37 – 56	20	H-T-H motif Potential

Sequences

	Sequence		Length	Mass (Da)
	P77245 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 4235E45DB8B7C3A8	FASTA	285	31,192
	10 20 30 40 50 60 MLYLTKISNA GSEFTENEQK IADFLQANVS ELQSVSSRQM AKQLGISQSS IVKFAQKLGA 70 80 90 100 110 120 QGFTELRMAL IGEYSASREK TNATALHLHS SITSDDSLEV IARKLNREKE LALEQTCALL 130 140 150 160 170 180 DYARLQKIIE VISKAPFIQI TGLGGSALVG RDLSFKLMKI GYRVACEADT HVQATVSQAL 190 200 210 220 230 240 KKGDVQIAIS YSGSKKEIVL CAEAARKQGA TVIAITSLTD SPLRRLAHFT LDTVSGETEW 250 260 270 280 RSSSMSTRTA QNSVTDLLFV GLVQLNDVES LKMIQRSSEL TQRLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The transcriptional factors MurR and catabolite activator protein regulate N-acetylmuramic acid catabolism in Escherichia coli." Jaeger T., Mayer C. J. Bacteriol. 190:6598-6608(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A TRANSCRIPTIONAL REGULATOR, PATHWAY, INDUCER, INDUCTION, SUBUNIT, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC75480.1. AP009048 Genomic DNA. Translation: BAA16310.1.
PIR	B65017.
RefSeq	NP_416922.1. NC_000913.2. YP_490663.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P77245.
SMR	P77245. Positions 4-70, 120-227.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12018N.
IntAct	P77245. 12 interactions.
STRING	511145.b2427.
Proteomic databases
PRIDE	P77245.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75480; AAC75480; b2427. BAA16310; BAA16310; BAA16310.
GeneID	12934053. 946568.
KEGG	ecj:Y75_p2388. eco:b2427.
PATRIC	32120239. VBIEscCol129921_2522.
Organism-specific databases
EchoBASE	EB3913.
EcoGene	EG14161. murR.
Phylogenomic databases
eggNOG	COG1737.
HOGENOM	HOG000027157.
KO	K15835.
OMA	AESEFTG.
ProtClustDB	PRK15482.
Enzyme and pathway databases
BioCyc	EcoCyc:G7262-MONOMER. ECOL316407:JW2420-MONOMER.
UniPathway	UPA00342.
Gene expression databases
Genevestigator	P77245.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_02108. HTH-type_MurR.
InterPro	IPR000281. HTH_RpiR. IPR001347. SIS. IPR022821. Tscrpt_reg_HTH_MurR. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01418. HTH_6. 1 hit. PF01380. SIS. 1 hit. [Graphical view]
PROSITE	PS51071. HTH_RPIR. 1 hit. PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURR_ECOLI

Accession

Primary (citable) accession number: P77245

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program