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Protein

HTH-type transcriptional regulator MurR

Gene

murR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Represses the expression of the murPQ operon involved in the uptake and degradation of N-acetylmuramic acid (MurNAc). Binds to two adjacent inverted repeats within the operator region. MurNAc 6-phosphate, the substrate of MurQ, is the specific inducer that weakens binding of MurR to the operator. Also represses its own transcription.1 Publication

Pathwayi: N-acetylmuramate degradation

This protein regulates the pathway N-acetylmuramate degradation, which is part of Amino-sugar metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway N-acetylmuramate degradation and in Amino-sugar metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi37 – 56H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Carbohydrate metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7262-MONOMER.
ECOL316407:JW2420-MONOMER.
UniPathwayiUPA00342.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator MurR
Alternative name(s):
MurPQ operon repressor
Gene namesi
Name:murR
Synonyms:yfeT
Ordered Locus Names:b2427, JW2420
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14161. murR.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show an abolition of the extensive lag phase observed when grown on MurNAc and a 20-fold enhancement of murQ transcription.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000686251 – 285HTH-type transcriptional regulator MurRAdd BLAST285

Proteomic databases

PaxDbiP77245.
PRIDEiP77245.

Expressioni

Inductioni

Repressed by itself and by the cAMP receptor protein crp.1 Publication

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi4263047. 28 interactors.
DIPiDIP-12018N.
IntActiP77245. 12 interactors.
STRINGi511145.b2427.

Structurei

3D structure databases

ProteinModelPortaliP77245.
SMRiP77245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 77HTH rpiR-typeAdd BLAST77
Domaini128 – 268SISAdd BLAST141

Sequence similaritiesi

Contains 1 SIS domain.Curated

Phylogenomic databases

eggNOGiENOG4108PSC. Bacteria.
COG1737. LUCA.
HOGENOMiHOG000027157.
InParanoidiP77245.
KOiK15835.
OMAiRFRTEAM.
PhylomeDBiP77245.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_02108. HTH_type_MurR. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR000281. HTH_RpiR.
IPR001347. SIS.
IPR022821. Tscrpt_reg_HTH_MurR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01418. HTH_6. 1 hit.
PF01380. SIS. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51071. HTH_RPIR. 1 hit.
PS51464. SIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYLTKISNA GSEFTENEQK IADFLQANVS ELQSVSSRQM AKQLGISQSS
60 70 80 90 100
IVKFAQKLGA QGFTELRMAL IGEYSASREK TNATALHLHS SITSDDSLEV
110 120 130 140 150
IARKLNREKE LALEQTCALL DYARLQKIIE VISKAPFIQI TGLGGSALVG
160 170 180 190 200
RDLSFKLMKI GYRVACEADT HVQATVSQAL KKGDVQIAIS YSGSKKEIVL
210 220 230 240 250
CAEAARKQGA TVIAITSLTD SPLRRLAHFT LDTVSGETEW RSSSMSTRTA
260 270 280
QNSVTDLLFV GLVQLNDVES LKMIQRSSEL TQRLK
Length:285
Mass (Da):31,192
Last modified:February 1, 1997 - v1
Checksum:i4235E45DB8B7C3A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75480.1.
AP009048 Genomic DNA. Translation: BAA16310.1.
PIRiB65017.
RefSeqiNP_416922.1. NC_000913.3.
WP_000966470.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75480; AAC75480; b2427.
BAA16310; BAA16310; BAA16310.
GeneIDi946568.
KEGGiecj:JW2420.
eco:b2427.
PATRICi32120239. VBIEscCol129921_2522.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75480.1.
AP009048 Genomic DNA. Translation: BAA16310.1.
PIRiB65017.
RefSeqiNP_416922.1. NC_000913.3.
WP_000966470.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77245.
SMRiP77245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263047. 28 interactors.
DIPiDIP-12018N.
IntActiP77245. 12 interactors.
STRINGi511145.b2427.

Proteomic databases

PaxDbiP77245.
PRIDEiP77245.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75480; AAC75480; b2427.
BAA16310; BAA16310; BAA16310.
GeneIDi946568.
KEGGiecj:JW2420.
eco:b2427.
PATRICi32120239. VBIEscCol129921_2522.

Organism-specific databases

EchoBASEiEB3913.
EcoGeneiEG14161. murR.

Phylogenomic databases

eggNOGiENOG4108PSC. Bacteria.
COG1737. LUCA.
HOGENOMiHOG000027157.
InParanoidiP77245.
KOiK15835.
OMAiRFRTEAM.
PhylomeDBiP77245.

Enzyme and pathway databases

UniPathwayiUPA00342.
BioCyciEcoCyc:G7262-MONOMER.
ECOL316407:JW2420-MONOMER.

Miscellaneous databases

PROiP77245.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_02108. HTH_type_MurR. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR000281. HTH_RpiR.
IPR001347. SIS.
IPR022821. Tscrpt_reg_HTH_MurR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01418. HTH_6. 1 hit.
PF01380. SIS. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51071. HTH_RPIR. 1 hit.
PS51464. SIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMURR_ECOLI
AccessioniPrimary (citable) accession number: P77245
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Neither GlcNAc-6-P, GlcNAc, anhydroMurNAc, MurNAc, nor muramyl dipeptide have an effect on MurR binding to the operator site.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.