ID   CUSB_ECOLI              Reviewed;         407 AA.
AC   P77239;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   24-JAN-2024, entry version 156.
DE   RecName: Full=Cation efflux system protein CusB;
DE   Flags: Precursor;
GN   Name=cusB; Synonyms=ylcD; OrderedLocusNames=b0574, JW0563;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   GENE NAME.
RC   STRAIN=K12 / DH5-alpha;
RX   PubMed=11004187; DOI=10.1128/jb.182.20.5864-5871.2000;
RA   Munson G.P., Lam D.L., Outten F.W., O'Halloran T.V.;
RT   "Identification of a copper-responsive two-component system on the
RT   chromosome of Escherichia coli K-12.";
RL   J. Bacteriol. 182:5864-5871(2000).
RN   [6]
RP   FUNCTION IN COPPER HOMEOSTASIS.
RC   STRAIN=K12;
RX   PubMed=11399769; DOI=10.1074/jbc.m104122200;
RA   Outten F.W., Huffman D.L., Hale J.A., O'Halloran T.V.;
RT   "The independent cue and cus systems confer copper tolerance during aerobic
RT   and anaerobic growth in Escherichia coli.";
RL   J. Biol. Chem. 276:30670-30677(2001).
RN   [7]
RP   INDUCTION.
RC   STRAIN=K38;
RX   PubMed=11283292; DOI=10.1099/00221287-147-4-965;
RA   Franke S., Grass G., Nies D.H.;
RT   "The product of the ybdE gene of the Escherichia coli chromosome is
RT   involved in detoxification of silver ions.";
RL   Microbiology 147:965-972(2001).
RN   [8]
RP   FUNCTION.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=12813074; DOI=10.1128/jb.185.13.3804-3812.2003;
RA   Franke S., Grass G., Rensing C., Nies D.H.;
RT   "Molecular analysis of the copper-transporting efflux system CusCFBA of
RT   Escherichia coli.";
RL   J. Bacteriol. 185:3804-3812(2003).
CC   -!- FUNCTION: Part of a cation efflux system that mediates resistance to
CC       copper and silver. {ECO:0000269|PubMed:11399769,
CC       ECO:0000269|PubMed:12813074}.
CC   -!- SUBUNIT: The cus efflux system is composed of CusA, CusB, CusC and
CC       CusF.
CC   -!- INTERACTION:
CC       P77239; P38054: cusA; NbExp=2; IntAct=EBI-1118842, EBI-1126317;
CC   -!- INDUCTION: Transcriptionally regulated by CusR in response to copper
CC       and silver ions. {ECO:0000269|PubMed:11283292}.
CC   -!- MISCELLANEOUS: The cus system plays an important role in copper
CC       tolerance under anaerobic growth and, under extreme copper stress, in
CC       aerobic growth.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; U82598; AAB40772.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73675.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35208.1; -; Genomic_DNA.
DR   PIR; D64790; D64790.
DR   RefSeq; NP_415106.1; NC_000913.3.
DR   RefSeq; WP_000717157.1; NZ_STEB01000055.1.
DR   PDB; 3H94; X-ray; 3.84 A; A/B=1-407.
DR   PDB; 3NE5; X-ray; 2.90 A; B/C=1-407.
DR   PDB; 3OOC; X-ray; 3.40 A; A/B=1-407.
DR   PDB; 3OPO; X-ray; 3.85 A; A/B=1-407.
DR   PDB; 3OW7; X-ray; 3.78 A; A/B=1-407.
DR   PDB; 3T51; X-ray; 3.90 A; B/C=78-407.
DR   PDB; 3T53; X-ray; 3.37 A; B/C=78-407.
DR   PDB; 3T56; X-ray; 3.42 A; B/C=78-407.
DR   PDB; 4DNR; X-ray; 3.68 A; B/C=1-407.
DR   PDB; 4DNT; X-ray; 3.10 A; B/C=1-407.
DR   PDB; 4DOP; X-ray; 4.20 A; B/C=1-407.
DR   PDBsum; 3H94; -.
DR   PDBsum; 3NE5; -.
DR   PDBsum; 3OOC; -.
DR   PDBsum; 3OPO; -.
DR   PDBsum; 3OW7; -.
DR   PDBsum; 3T51; -.
DR   PDBsum; 3T53; -.
DR   PDBsum; 3T56; -.
DR   PDBsum; 4DNR; -.
DR   PDBsum; 4DNT; -.
DR   PDBsum; 4DOP; -.
DR   AlphaFoldDB; P77239; -.
DR   SMR; P77239; -.
DR   BioGRID; 4262993; 137.
DR   BioGRID; 849575; 1.
DR   ComplexPortal; CPX-2254; Cus cation efflux complex.
DR   DIP; DIP-9346N; -.
DR   IntAct; P77239; 5.
DR   STRING; 511145.b0574; -.
DR   TCDB; 2.A.6.1.4; the resistance-nodulation-cell division (rnd) superfamily.
DR   PaxDb; 511145-b0574; -.
DR   EnsemblBacteria; AAC73675; AAC73675; b0574.
DR   GeneID; 945189; -.
DR   KEGG; ecj:JW0563; -.
DR   KEGG; eco:b0574; -.
DR   PATRIC; fig|1411691.4.peg.1700; -.
DR   EchoBASE; EB3986; -.
DR   eggNOG; COG0845; Bacteria.
DR   HOGENOM; CLU_018816_13_1_6; -.
DR   InParanoid; P77239; -.
DR   OMA; WLIKDAS; -.
DR   OrthoDB; 9806939at2; -.
DR   PhylomeDB; P77239; -.
DR   BioCyc; EcoCyc:G6322-MONOMER; -.
DR   BioCyc; MetaCyc:G6322-MONOMER; -.
DR   EvolutionaryTrace; P77239; -.
DR   PRO; PR:P77239; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0030313; C:cell envelope; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR   GO; GO:0005507; F:copper ion binding; IDA:EcoCyc.
DR   GO; GO:0005375; F:copper ion transmembrane transporter activity; IGI:EcoCyc.
DR   GO; GO:0046914; F:transition metal ion binding; IDA:EcoCyc.
DR   GO; GO:0060003; P:copper ion export; IGI:EcoCyc.
DR   GO; GO:0035434; P:copper ion transmembrane transport; NAS:ComplexPortal.
DR   GO; GO:0010273; P:detoxification of copper ion; IMP:EcoCyc.
DR   GO; GO:0006878; P:intracellular copper ion homeostasis; IMP:EcoCyc.
DR   GO; GO:0015679; P:plasma membrane copper ion transport; IGI:EcoCyc.
DR   GO; GO:0046688; P:response to copper ion; IMP:EcoliWiki.
DR   GO; GO:0010272; P:response to silver ion; IMP:EcoCyc.
DR   GO; GO:0009636; P:response to toxic substance; NAS:ComplexPortal.
DR   GO; GO:1902601; P:silver ion transmembrane transport; NAS:ComplexPortal.
DR   Gene3D; 2.40.30.170; -; 1.
DR   Gene3D; 2.40.420.20; -; 1.
DR   Gene3D; 6.10.140.730; -; 1.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR045800; HMBD.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   NCBIfam; TIGR01730; RND_mfp; 1.
DR   PANTHER; PTHR30097; CATION EFFLUX SYSTEM PROTEIN CUSB; 1.
DR   PANTHER; PTHR30097:SF15; CATION EFFLUX SYSTEM PROTEIN CUSB; 1.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   Pfam; PF19335; HMBD; 1.
DR   SUPFAM; SSF111369; HlyD-like secretion proteins; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Copper; Copper transport; Ion transport; Reference proteome;
KW   Signal; Transport.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..407
FT                   /note="Cation efflux system protein CusB"
FT                   /id="PRO_0000018690"
FT   STRAND          96..98
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          100..102
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          105..124
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          130..135
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          143..145
FT                   /evidence="ECO:0007829|PDB:3OOC"
FT   STRAND          149..154
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   HELIX           159..170
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   HELIX           175..187
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   HELIX           192..201
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          207..211
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          213..220
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          231..233
FT                   /evidence="ECO:0007829|PDB:3T56"
FT   STRAND          235..251
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   HELIX           252..254
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   HELIX           255..258
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   HELIX           261..263
FT                   /evidence="ECO:0007829|PDB:3OOC"
FT   STRAND          264..268
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          271..278
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          282..288
FT                   /evidence="ECO:0007829|PDB:4DNT"
FT   TURN            289..292
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          293..301
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          303..305
FT                   /evidence="ECO:0007829|PDB:3OOC"
FT   STRAND          312..320
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          324..328
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   HELIX           329..331
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          332..334
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          335..337
FT                   /evidence="ECO:0007829|PDB:3OOC"
FT   STRAND          339..344
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          346..348
FT                   /evidence="ECO:0007829|PDB:3T53"
FT   STRAND          350..354
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          356..361
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          364..371
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   STRAND          376..378
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   TURN            386..388
FT                   /evidence="ECO:0007829|PDB:3NE5"
FT   HELIX           391..396
FT                   /evidence="ECO:0007829|PDB:3NE5"
SQ   SEQUENCE   407 AA;  44305 MW;  E949390000C61FC6 CRC64;
     MKKIALIIGS MIAGGIISAA GFTWVAKAEP PAEKTSTAER KILFWYDPMY PNTRFDKPGK
     SPFMDMDLVP KYADEESSAS GVRIDPTQTQ NLGVKTATVT RGPLTFAQSF PANVSYNEYQ
     YAIVQARAAG FIDKVYPLTV GDKVQKGTPL LDLTIPDWVE AQSEYLLLRE TGGTATQTEG
     ILERLRLAGM PEADIRRLIA TQKIQTRFTL KAPIDGVITA FDLRAGMNIA KDNVVAKIQG
     MDPVWVTAAI PESIAWLVKD ASQFTLTVPA RPDKTLTIRK WTLLPGVDAA TRTLQLRLEV
     DNADEALKPG MNAWLQLNTA SEPMLLIPSQ ALIDTGSEQR VITVDADGRF VPKRVAVFQA
     SQGVTALRSG LAEGEKVVSS GLFLIDSEAN ISGALERMRS ESATHAH
//