Cation efflux system protein CusB precursor - Escherichia coli (strain K12)

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P77239 (CUSB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cation efflux system protein CusB

Gene names

Name:	cusB
Synonyms:	ylcD
Ordered Locus Names:	b0574, JW0563

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of a cation efflux system that mediates resistance to copper and silver. Ref.6 Ref.8
Subunit structure	The cus efflux system is composed of CusA, CusB, CusC and CusF.
Induction	Transcriptionally regulated by CusR in response to copper and silver ions. Ref.7
Miscellaneous	The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.
Sequence similarities	Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family. [View classification]

Ontologies

Keywords
Biological process	Copper transport Ion transport Transport
Domain	Signal
Ligand	Copper
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular copper ion homeostasis Inferred from mutant phenotype PubMed 17893146. Source: EcoCyc copper ion export Inferred from genetic interaction Ref.8. Source: EcoCyc detoxification of copper ion Inferred from mutant phenotype PubMed 17893146. Source: EcoCyc plasma membrane copper ion transport Inferred from genetic interaction Ref.8. Source: EcoCyc response to silver ion Inferred from expression pattern Ref.7 PubMed 18419149. Source: EcoCyc
Cellular_component	membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	copper ion binding Inferred from direct assay PubMed 17893146. Source: EcoCyc copper ion transmembrane transporter activity Inferred from genetic interaction Ref.8. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

Potential

Chain

29 – 407

379

Cation efflux system protein CusB

PRO_0000018690

Secondary structure

407

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P77239 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: E949390000C61FC6
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FASTA

407

44,305

        10         20         30         40         50         60 
MKKIALIIGS MIAGGIISAA GFTWVAKAEP PAEKTSTAER KILFWYDPMY PNTRFDKPGK 

        70         80         90        100        110        120 
SPFMDMDLVP KYADEESSAS GVRIDPTQTQ NLGVKTATVT RGPLTFAQSF PANVSYNEYQ 

       130        140        150        160        170        180 
YAIVQARAAG FIDKVYPLTV GDKVQKGTPL LDLTIPDWVE AQSEYLLLRE TGGTATQTEG 

       190        200        210        220        230        240 
ILERLRLAGM PEADIRRLIA TQKIQTRFTL KAPIDGVITA FDLRAGMNIA KDNVVAKIQG 

       250        260        270        280        290        300 
MDPVWVTAAI PESIAWLVKD ASQFTLTVPA RPDKTLTIRK WTLLPGVDAA TRTLQLRLEV 

       310        320        330        340        350        360 
DNADEALKPG MNAWLQLNTA SEPMLLIPSQ ALIDTGSEQR VITVDADGRF VPKRVAVFQA 

       370        380        390        400 
SQGVTALRSG LAEGEKVVSS GLFLIDSEAN ISGALERMRS ESATHAH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12." Munson G.P., Lam D.L., Outten F.W., O'Halloran T.V. J. Bacteriol. 182:5864-5871(2000) [PubMed] [Europe PMC] [Abstract] Cited for: GENE NAME. Strain: K12 / DH5-alpha.
[6]	"The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli." Outten F.W., Huffman D.L., Hale J.A., O'Halloran T.V. J. Biol. Chem. 276:30670-30677(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN COPPER HOMEOSTASIS. Strain: K12.
[7]	"The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions." Franke S., Grass G., Nies D.H. Microbiology 147:965-972(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K38.
[8]	"Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli." Franke S., Grass G., Rensing C., Nies D.H. J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U82598 Genomic DNA. Translation: AAB40772.1.
U00096 Genomic DNA. Translation: AAC73675.1.
AP009048 Genomic DNA. Translation: BAA35208.1.

PIR

D64790.

RefSeq

NP_415106.1. NC_000913.2.
YP_488861.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3H94	X-ray	3.84	A/B	1-407	[»]
3NE5	X-ray	2.90	B/C	1-407	[»]
3OOC	X-ray	3.40	A/B	1-407	[»]
3OPO	X-ray	3.85	A/B	1-407	[»]
3OW7	X-ray	3.78	A/B	1-407	[»]
3T51	X-ray	3.90	B/C	78-407	[»]
3T53	X-ray	3.37	B/C	78-407	[»]
3T56	X-ray	3.42	B/C	78-407	[»]
4DNR	X-ray	3.68	B/C	1-407	[»]
4DNT	X-ray	3.10	B/C	1-407	[»]
4DOP	X-ray	4.20	B/C	1-407	[»]

ProteinModelPortal

P77239.

SMR

P77239. Positions 79-402.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9346N.

IntAct

P77239. 2 interactions.

STRING

511145.b0574.

Protein family/group databases

TCDB

2.A.6.1.4. resistance-nodulation-cell division (RND) superfamily.

Proteomic databases

PaxDb

P77239.

PRIDE

P77239.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73675; AAC73675; b0574.
BAA35208; BAA35208; BAA35208.

GeneID

12933999.
945189.

KEGG

ecj:Y75_p0561.
eco:b0574.

PATRIC

32116316. VBIEscCol129921_0598.

Organism-specific databases

EchoBASE

EB3986.

EcoGene

EG14235. cusB.

Phylogenomic databases

eggNOG

COG0845.

HOGENOM

HOG000126091.

K07798.

OMA

PKTRTLR.

ProtClustDB

PRK09783.

Enzyme and pathway databases

BioCyc

EcoCyc:G6322-MONOMER.
ECOL316407:JW0563-MONOMER.

Gene expression databases

Genevestigator

P77239.

Family and domain databases

InterPro

IPR006143. RND_pump_MFP.
[Graphical view]

TIGRFAMs

TIGR01730. RND_mfp. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P77239.

Entry information

Entry name

CUSB_ECOLI

Accession

Primary (citable) accession number: P77239

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents