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P77239

- CUSB_ECOLI

UniProt

P77239 - CUSB_ECOLI

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Protein

Cation efflux system protein CusB

Gene

cusB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Part of a cation efflux system that mediates resistance to copper and silver.2 Publications

GO - Molecular functioni

  1. copper ion binding Source: EcoCyc
  2. copper ion transmembrane transporter activity Source: EcoCyc
  3. transition metal ion binding Source: EcoCyc

GO - Biological processi

  1. cellular copper ion homeostasis Source: EcoCyc
  2. copper ion export Source: EcoCyc
  3. detoxification of copper ion Source: EcoCyc
  4. plasma membrane copper ion transport Source: EcoCyc
  5. response to copper ion Source: EcoCyc
  6. response to silver ion Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Copper transport, Ion transport, Transport

Keywords - Ligandi

Copper

Enzyme and pathway databases

BioCyciEcoCyc:G6322-MONOMER.
ECOL316407:JW0563-MONOMER.
MetaCyc:G6322-MONOMER.

Protein family/group databases

TCDBi2.A.6.1.4. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cation efflux system protein CusB
Gene namesi
Name:cusB
Synonyms:ylcD
Ordered Locus Names:b0574, JW0563
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG14235. cusB.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: InterPro
  2. outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 407379Cation efflux system protein CusBPRO_0000018690Add
BLAST

Proteomic databases

PaxDbiP77239.
PRIDEiP77239.

Expressioni

Inductioni

Transcriptionally regulated by CusR in response to copper and silver ions.1 Publication

Gene expression databases

GenevestigatoriP77239.

Interactioni

Subunit structurei

The cus efflux system is composed of CusA, CusB, CusC and CusF.

Protein-protein interaction databases

DIPiDIP-9346N.
IntActiP77239. 3 interactions.
STRINGi511145.b0574.

Structurei

Secondary structure

1
407
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi96 – 983
Beta strandi100 – 1023
Beta strandi105 – 12420
Beta strandi130 – 1356
Beta strandi143 – 1453
Beta strandi149 – 1546
Helixi159 – 17012
Helixi175 – 18713
Helixi192 – 20110
Beta strandi207 – 2115
Beta strandi213 – 2208
Beta strandi231 – 2333
Beta strandi235 – 25117
Helixi252 – 2543
Helixi255 – 2584
Helixi261 – 2633
Beta strandi264 – 2685
Beta strandi271 – 2788
Beta strandi282 – 2887
Turni289 – 2924
Beta strandi293 – 3019
Beta strandi303 – 3053
Beta strandi312 – 3209
Beta strandi324 – 3285
Helixi329 – 3313
Beta strandi332 – 3343
Beta strandi335 – 3373
Beta strandi339 – 3446
Beta strandi346 – 3483
Beta strandi350 – 3545
Beta strandi356 – 3616
Beta strandi364 – 3718
Beta strandi376 – 3783
Turni386 – 3883
Helixi391 – 3966

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H94X-ray3.84A/B1-407[»]
3NE5X-ray2.90B/C1-407[»]
3OOCX-ray3.40A/B1-407[»]
3OPOX-ray3.85A/B1-407[»]
3OW7X-ray3.78A/B1-407[»]
3T51X-ray3.90B/C78-407[»]
3T53X-ray3.37B/C78-407[»]
3T56X-ray3.42B/C78-407[»]
4DNRX-ray3.68B/C1-407[»]
4DNTX-ray3.10B/C1-407[»]
4DOPX-ray4.20B/C1-407[»]
ProteinModelPortaliP77239.
SMRiP77239. Positions 79-402.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77239.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0845.
HOGENOMiHOG000126091.
InParanoidiP77239.
KOiK07798.
OMAiFHESAGV.
OrthoDBiEOG693GPF.
PhylomeDBiP77239.

Family and domain databases

InterProiIPR006143. RND_pump_MFP.
[Graphical view]
TIGRFAMsiTIGR01730. RND_mfp. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77239-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKIALIIGS MIAGGIISAA GFTWVAKAEP PAEKTSTAER KILFWYDPMY
60 70 80 90 100
PNTRFDKPGK SPFMDMDLVP KYADEESSAS GVRIDPTQTQ NLGVKTATVT
110 120 130 140 150
RGPLTFAQSF PANVSYNEYQ YAIVQARAAG FIDKVYPLTV GDKVQKGTPL
160 170 180 190 200
LDLTIPDWVE AQSEYLLLRE TGGTATQTEG ILERLRLAGM PEADIRRLIA
210 220 230 240 250
TQKIQTRFTL KAPIDGVITA FDLRAGMNIA KDNVVAKIQG MDPVWVTAAI
260 270 280 290 300
PESIAWLVKD ASQFTLTVPA RPDKTLTIRK WTLLPGVDAA TRTLQLRLEV
310 320 330 340 350
DNADEALKPG MNAWLQLNTA SEPMLLIPSQ ALIDTGSEQR VITVDADGRF
360 370 380 390 400
VPKRVAVFQA SQGVTALRSG LAEGEKVVSS GLFLIDSEAN ISGALERMRS

ESATHAH
Length:407
Mass (Da):44,305
Last modified:February 1, 1997 - v1
Checksum:iE949390000C61FC6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U82598 Genomic DNA. Translation: AAB40772.1.
U00096 Genomic DNA. Translation: AAC73675.1.
AP009048 Genomic DNA. Translation: BAA35208.1.
PIRiD64790.
RefSeqiNP_415106.1. NC_000913.3.
YP_488861.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73675; AAC73675; b0574.
BAA35208; BAA35208; BAA35208.
GeneIDi12933999.
945189.
KEGGiecj:Y75_p0561.
eco:b0574.
PATRICi32116316. VBIEscCol129921_0598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U82598 Genomic DNA. Translation: AAB40772.1 .
U00096 Genomic DNA. Translation: AAC73675.1 .
AP009048 Genomic DNA. Translation: BAA35208.1 .
PIRi D64790.
RefSeqi NP_415106.1. NC_000913.3.
YP_488861.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3H94 X-ray 3.84 A/B 1-407 [» ]
3NE5 X-ray 2.90 B/C 1-407 [» ]
3OOC X-ray 3.40 A/B 1-407 [» ]
3OPO X-ray 3.85 A/B 1-407 [» ]
3OW7 X-ray 3.78 A/B 1-407 [» ]
3T51 X-ray 3.90 B/C 78-407 [» ]
3T53 X-ray 3.37 B/C 78-407 [» ]
3T56 X-ray 3.42 B/C 78-407 [» ]
4DNR X-ray 3.68 B/C 1-407 [» ]
4DNT X-ray 3.10 B/C 1-407 [» ]
4DOP X-ray 4.20 B/C 1-407 [» ]
ProteinModelPortali P77239.
SMRi P77239. Positions 79-402.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9346N.
IntActi P77239. 3 interactions.
STRINGi 511145.b0574.

Protein family/group databases

TCDBi 2.A.6.1.4. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbi P77239.
PRIDEi P77239.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73675 ; AAC73675 ; b0574 .
BAA35208 ; BAA35208 ; BAA35208 .
GeneIDi 12933999.
945189.
KEGGi ecj:Y75_p0561.
eco:b0574.
PATRICi 32116316. VBIEscCol129921_0598.

Organism-specific databases

EchoBASEi EB3986.
EcoGenei EG14235. cusB.

Phylogenomic databases

eggNOGi COG0845.
HOGENOMi HOG000126091.
InParanoidi P77239.
KOi K07798.
OMAi FHESAGV.
OrthoDBi EOG693GPF.
PhylomeDBi P77239.

Enzyme and pathway databases

BioCyci EcoCyc:G6322-MONOMER.
ECOL316407:JW0563-MONOMER.
MetaCyc:G6322-MONOMER.

Miscellaneous databases

EvolutionaryTracei P77239.
PROi P77239.

Gene expression databases

Genevestigatori P77239.

Family and domain databases

InterProi IPR006143. RND_pump_MFP.
[Graphical view ]
TIGRFAMsi TIGR01730. RND_mfp. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12."
    Munson G.P., Lam D.L., Outten F.W., O'Halloran T.V.
    J. Bacteriol. 182:5864-5871(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
    Strain: K12 / DH5-alpha.
  6. "The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli."
    Outten F.W., Huffman D.L., Hale J.A., O'Halloran T.V.
    J. Biol. Chem. 276:30670-30677(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN COPPER HOMEOSTASIS.
    Strain: K12.
  7. "The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions."
    Franke S., Grass G., Nies D.H.
    Microbiology 147:965-972(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K38.
  8. "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli."
    Franke S., Grass G., Rensing C., Nies D.H.
    J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiCUSB_ECOLI
AccessioniPrimary (citable) accession number: P77239
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3