Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P77239 (CUSB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cation efflux system protein CusB
Gene names
Name:cusB
Synonyms:ylcD
Ordered Locus Names:b0574, JW0563
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of a cation efflux system that mediates resistance to copper and silver. Ref.6 Ref.8

Subunit structure

The cus efflux system is composed of CusA, CusB, CusC and CusF.

Induction

Transcriptionally regulated by CusR in response to copper and silver ions. Ref.7

Miscellaneous

The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.

Sequence similarities

Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 407379Cation efflux system protein CusB
PRO_0000018690

Secondary structure

............................................................... 407
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P77239 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: E949390000C61FC6

FASTA40744,305
        10         20         30         40         50         60 
MKKIALIIGS MIAGGIISAA GFTWVAKAEP PAEKTSTAER KILFWYDPMY PNTRFDKPGK 

        70         80         90        100        110        120 
SPFMDMDLVP KYADEESSAS GVRIDPTQTQ NLGVKTATVT RGPLTFAQSF PANVSYNEYQ 

       130        140        150        160        170        180 
YAIVQARAAG FIDKVYPLTV GDKVQKGTPL LDLTIPDWVE AQSEYLLLRE TGGTATQTEG 

       190        200        210        220        230        240 
ILERLRLAGM PEADIRRLIA TQKIQTRFTL KAPIDGVITA FDLRAGMNIA KDNVVAKIQG 

       250        260        270        280        290        300 
MDPVWVTAAI PESIAWLVKD ASQFTLTVPA RPDKTLTIRK WTLLPGVDAA TRTLQLRLEV 

       310        320        330        340        350        360 
DNADEALKPG MNAWLQLNTA SEPMLLIPSQ ALIDTGSEQR VITVDADGRF VPKRVAVFQA 

       370        380        390        400 
SQGVTALRSG LAEGEKVVSS GLFLIDSEAN ISGALERMRS ESATHAH 

« Hide

References

« Hide 'large scale' references
[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12."
Munson G.P., Lam D.L., Outten F.W., O'Halloran T.V.
J. Bacteriol. 182:5864-5871(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE NAME.
Strain: K12 / DH5-alpha.
[6]"The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli."
Outten F.W., Huffman D.L., Hale J.A., O'Halloran T.V.
J. Biol. Chem. 276:30670-30677(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN COPPER HOMEOSTASIS.
Strain: K12.
[7]"The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions."
Franke S., Grass G., Nies D.H.
Microbiology 147:965-972(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: K38.
[8]"Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli."
Franke S., Grass G., Rensing C., Nies D.H.
J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U82598 Genomic DNA. Translation: AAB40772.1.
U00096 Genomic DNA. Translation: AAC73675.1.
AP009048 Genomic DNA. Translation: BAA35208.1.
PIRD64790.
RefSeqNP_415106.1. NC_000913.3.
YP_488861.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3H94X-ray3.84A/B1-407[»]
3NE5X-ray2.90B/C1-407[»]
3OOCX-ray3.40A/B1-407[»]
3OPOX-ray3.85A/B1-407[»]
3OW7X-ray3.78A/B1-407[»]
3T51X-ray3.90B/C78-407[»]
3T53X-ray3.37B/C78-407[»]
3T56X-ray3.42B/C78-407[»]
4DNRX-ray3.68B/C1-407[»]
4DNTX-ray3.10B/C1-407[»]
4DOPX-ray4.20B/C1-407[»]
ProteinModelPortalP77239.
SMRP77239. Positions 79-402.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9346N.
IntActP77239. 3 interactions.
STRING511145.b0574.

Protein family/group databases

TCDB2.A.6.1.4. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbP77239.
PRIDEP77239.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73675; AAC73675; b0574.
BAA35208; BAA35208; BAA35208.
GeneID12933999.
945189.
KEGGecj:Y75_p0561.
eco:b0574.
PATRIC32116316. VBIEscCol129921_0598.

Organism-specific databases

EchoBASEEB3986.
EcoGeneEG14235. cusB.

Phylogenomic databases

eggNOGCOG0845.
HOGENOMHOG000126091.
KOK07798.
OMAFHESAGV.
OrthoDBEOG693GPF.
PhylomeDBP77239.

Enzyme and pathway databases

BioCycEcoCyc:G6322-MONOMER.
ECOL316407:JW0563-MONOMER.
MetaCyc:G6322-MONOMER.

Gene expression databases

GenevestigatorP77239.

Family and domain databases

InterProIPR006143. RND_pump_MFP.
[Graphical view]
TIGRFAMsTIGR01730. RND_mfp. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP77239.
PROP77239.

Entry information

Entry nameCUSB_ECOLI
AccessionPrimary (citable) accession number: P77239
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene