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P77239

- CUSB_ECOLI

UniProt

P77239 - CUSB_ECOLI

Protein

Cation efflux system protein CusB

Gene

cusB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Part of a cation efflux system that mediates resistance to copper and silver.2 Publications

    GO - Molecular functioni

    1. copper ion binding Source: EcoCyc
    2. copper ion transmembrane transporter activity Source: EcoCyc
    3. protein binding Source: EcoCyc
    4. transition metal ion binding Source: EcoCyc

    GO - Biological processi

    1. cellular copper ion homeostasis Source: EcoCyc
    2. copper ion export Source: EcoCyc
    3. detoxification of copper ion Source: EcoCyc
    4. plasma membrane copper ion transport Source: EcoCyc
    5. response to copper ion Source: EcoCyc
    6. response to silver ion Source: EcoCyc

    Keywords - Biological processi

    Copper transport, Ion transport, Transport

    Keywords - Ligandi

    Copper

    Enzyme and pathway databases

    BioCyciEcoCyc:G6322-MONOMER.
    ECOL316407:JW0563-MONOMER.
    MetaCyc:G6322-MONOMER.

    Protein family/group databases

    TCDBi2.A.6.1.4. the resistance-nodulation-cell division (rnd) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cation efflux system protein CusB
    Gene namesi
    Name:cusB
    Synonyms:ylcD
    Ordered Locus Names:b0574, JW0563
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG14235. cusB.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: InterPro
    2. outer membrane-bounded periplasmic space Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 407379Cation efflux system protein CusBPRO_0000018690Add
    BLAST

    Proteomic databases

    PaxDbiP77239.
    PRIDEiP77239.

    Expressioni

    Inductioni

    Transcriptionally regulated by CusR in response to copper and silver ions.1 Publication

    Gene expression databases

    GenevestigatoriP77239.

    Interactioni

    Subunit structurei

    The cus efflux system is composed of CusA, CusB, CusC and CusF.

    Protein-protein interaction databases

    DIPiDIP-9346N.
    IntActiP77239. 3 interactions.
    STRINGi511145.b0574.

    Structurei

    Secondary structure

    1
    407
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi96 – 983
    Beta strandi100 – 1023
    Beta strandi105 – 12420
    Beta strandi130 – 1356
    Beta strandi143 – 1453
    Beta strandi149 – 1546
    Helixi159 – 17012
    Helixi175 – 18713
    Helixi192 – 20110
    Beta strandi207 – 2115
    Beta strandi213 – 2208
    Beta strandi231 – 2333
    Beta strandi235 – 25117
    Helixi252 – 2543
    Helixi255 – 2584
    Helixi261 – 2633
    Beta strandi264 – 2685
    Beta strandi271 – 2788
    Beta strandi282 – 2887
    Turni289 – 2924
    Beta strandi293 – 3019
    Beta strandi303 – 3053
    Beta strandi312 – 3209
    Beta strandi324 – 3285
    Helixi329 – 3313
    Beta strandi332 – 3343
    Beta strandi335 – 3373
    Beta strandi339 – 3446
    Beta strandi346 – 3483
    Beta strandi350 – 3545
    Beta strandi356 – 3616
    Beta strandi364 – 3718
    Beta strandi376 – 3783
    Turni386 – 3883
    Helixi391 – 3966

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3H94X-ray3.84A/B1-407[»]
    3NE5X-ray2.90B/C1-407[»]
    3OOCX-ray3.40A/B1-407[»]
    3OPOX-ray3.85A/B1-407[»]
    3OW7X-ray3.78A/B1-407[»]
    3T51X-ray3.90B/C78-407[»]
    3T53X-ray3.37B/C78-407[»]
    3T56X-ray3.42B/C78-407[»]
    4DNRX-ray3.68B/C1-407[»]
    4DNTX-ray3.10B/C1-407[»]
    4DOPX-ray4.20B/C1-407[»]
    ProteinModelPortaliP77239.
    SMRiP77239. Positions 79-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP77239.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0845.
    HOGENOMiHOG000126091.
    KOiK07798.
    OMAiFHESAGV.
    OrthoDBiEOG693GPF.
    PhylomeDBiP77239.

    Family and domain databases

    InterProiIPR006143. RND_pump_MFP.
    [Graphical view]
    TIGRFAMsiTIGR01730. RND_mfp. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P77239-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKIALIIGS MIAGGIISAA GFTWVAKAEP PAEKTSTAER KILFWYDPMY    50
    PNTRFDKPGK SPFMDMDLVP KYADEESSAS GVRIDPTQTQ NLGVKTATVT 100
    RGPLTFAQSF PANVSYNEYQ YAIVQARAAG FIDKVYPLTV GDKVQKGTPL 150
    LDLTIPDWVE AQSEYLLLRE TGGTATQTEG ILERLRLAGM PEADIRRLIA 200
    TQKIQTRFTL KAPIDGVITA FDLRAGMNIA KDNVVAKIQG MDPVWVTAAI 250
    PESIAWLVKD ASQFTLTVPA RPDKTLTIRK WTLLPGVDAA TRTLQLRLEV 300
    DNADEALKPG MNAWLQLNTA SEPMLLIPSQ ALIDTGSEQR VITVDADGRF 350
    VPKRVAVFQA SQGVTALRSG LAEGEKVVSS GLFLIDSEAN ISGALERMRS 400
    ESATHAH 407
    Length:407
    Mass (Da):44,305
    Last modified:February 1, 1997 - v1
    Checksum:iE949390000C61FC6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U82598 Genomic DNA. Translation: AAB40772.1.
    U00096 Genomic DNA. Translation: AAC73675.1.
    AP009048 Genomic DNA. Translation: BAA35208.1.
    PIRiD64790.
    RefSeqiNP_415106.1. NC_000913.3.
    YP_488861.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73675; AAC73675; b0574.
    BAA35208; BAA35208; BAA35208.
    GeneIDi12933999.
    945189.
    KEGGiecj:Y75_p0561.
    eco:b0574.
    PATRICi32116316. VBIEscCol129921_0598.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U82598 Genomic DNA. Translation: AAB40772.1 .
    U00096 Genomic DNA. Translation: AAC73675.1 .
    AP009048 Genomic DNA. Translation: BAA35208.1 .
    PIRi D64790.
    RefSeqi NP_415106.1. NC_000913.3.
    YP_488861.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3H94 X-ray 3.84 A/B 1-407 [» ]
    3NE5 X-ray 2.90 B/C 1-407 [» ]
    3OOC X-ray 3.40 A/B 1-407 [» ]
    3OPO X-ray 3.85 A/B 1-407 [» ]
    3OW7 X-ray 3.78 A/B 1-407 [» ]
    3T51 X-ray 3.90 B/C 78-407 [» ]
    3T53 X-ray 3.37 B/C 78-407 [» ]
    3T56 X-ray 3.42 B/C 78-407 [» ]
    4DNR X-ray 3.68 B/C 1-407 [» ]
    4DNT X-ray 3.10 B/C 1-407 [» ]
    4DOP X-ray 4.20 B/C 1-407 [» ]
    ProteinModelPortali P77239.
    SMRi P77239. Positions 79-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9346N.
    IntActi P77239. 3 interactions.
    STRINGi 511145.b0574.

    Protein family/group databases

    TCDBi 2.A.6.1.4. the resistance-nodulation-cell division (rnd) superfamily.

    Proteomic databases

    PaxDbi P77239.
    PRIDEi P77239.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73675 ; AAC73675 ; b0574 .
    BAA35208 ; BAA35208 ; BAA35208 .
    GeneIDi 12933999.
    945189.
    KEGGi ecj:Y75_p0561.
    eco:b0574.
    PATRICi 32116316. VBIEscCol129921_0598.

    Organism-specific databases

    EchoBASEi EB3986.
    EcoGenei EG14235. cusB.

    Phylogenomic databases

    eggNOGi COG0845.
    HOGENOMi HOG000126091.
    KOi K07798.
    OMAi FHESAGV.
    OrthoDBi EOG693GPF.
    PhylomeDBi P77239.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6322-MONOMER.
    ECOL316407:JW0563-MONOMER.
    MetaCyc:G6322-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P77239.
    PROi P77239.

    Gene expression databases

    Genevestigatori P77239.

    Family and domain databases

    InterProi IPR006143. RND_pump_MFP.
    [Graphical view ]
    TIGRFAMsi TIGR01730. RND_mfp. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12."
      Munson G.P., Lam D.L., Outten F.W., O'Halloran T.V.
      J. Bacteriol. 182:5864-5871(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE NAME.
      Strain: K12 / DH5-alpha.
    6. "The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli."
      Outten F.W., Huffman D.L., Hale J.A., O'Halloran T.V.
      J. Biol. Chem. 276:30670-30677(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN COPPER HOMEOSTASIS.
      Strain: K12.
    7. "The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions."
      Franke S., Grass G., Nies D.H.
      Microbiology 147:965-972(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K38.
    8. "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli."
      Franke S., Grass G., Rensing C., Nies D.H.
      J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiCUSB_ECOLI
    AccessioniPrimary (citable) accession number: P77239
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3