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Protein

Electron transport complex subunit RsxB

Gene

rsxB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of a membrane complex involved in electron transport. Required to maintain the reduced state of SoxR.UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491Iron-sulfur 1 (2Fe-2S)UniRule annotation
Metal bindingi52 – 521Iron-sulfur 1 (2Fe-2S)UniRule annotation
Metal bindingi57 – 571Iron-sulfur 1 (2Fe-2S)UniRule annotation
Metal bindingi74 – 741Iron-sulfur 1 (2Fe-2S)UniRule annotation
Metal bindingi117 – 1171Iron-sulfur 2 (4Fe-4S)UniRule annotation
Metal bindingi120 – 1201Iron-sulfur 2 (4Fe-4S)UniRule annotation
Metal bindingi123 – 1231Iron-sulfur 2 (4Fe-4S)UniRule annotation
Metal bindingi127 – 1271Iron-sulfur 3 (4Fe-4S)UniRule annotation
Metal bindingi147 – 1471Iron-sulfur 3 (4Fe-4S)UniRule annotation
Metal bindingi150 – 1501Iron-sulfur 3 (4Fe-4S)UniRule annotation
Metal bindingi153 – 1531Iron-sulfur 3 (4Fe-4S)UniRule annotation
Metal bindingi157 – 1571Iron-sulfur 2 (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. electron carrier activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. oxidoreductase activity Source: GO_Central

GO - Biological processi

  1. electron transport chain Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6872-MONOMER.
ECOL316407:JW1620-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Electron transport complex subunit RsxBUniRule annotation
Gene namesi
Name:rsxBUniRule annotation
Synonyms:rnfB, ydgM
Ordered Locus Names:b1628, JW1620
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13934. rsxB.

Subcellular locationi

Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Electron transport complex subunit RsxBPRO_0000216271Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP77223.

Interactioni

Subunit structurei

Composed of six subunits; RsxA, RsxB, RsxC, RsxD, RsxE and RsxG.1 Publication

Protein-protein interaction databases

IntActiP77223. 1 interaction.
STRINGi511145.b1628.

Structurei

3D structure databases

ProteinModelPortaliP77223.
SMRiP77223. Positions 112-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 91604Fe-4SAdd
BLAST
Domaini108 – 137304Fe-4S ferredoxin-type 1UniRule annotationAdd
BLAST
Domaini138 – 167304Fe-4S ferredoxin-type 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfB subfamily.UniRule annotation
Contains 1 4Fe-4S domain.Curated
Contains 2 4Fe-4S ferredoxin-type domains.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2878.
HOGENOMiHOG000262938.
InParanoidiP77223.
KOiK03616.
OMAiKVEGDPI.
OrthoDBiEOG60SCM3.
PhylomeDBiP77223.

Family and domain databases

HAMAPiMF_00463. RsxB_RnfB.
InterProiIPR007202. 4Fe-4S_dom.
IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010207. Elect_transpt_cplx_RnfB.
IPR016463. RnfB_Proteobac.
[Graphical view]
PfamiPF00037. Fer4. 2 hits.
PF04060. FeS. 1 hit.
[Graphical view]
PIRSFiPIRSF005784. Elect_transpt_RnfB. 1 hit.
TIGRFAMsiTIGR01944. rnfB. 1 hit.
PROSITEiPS51656. 4FE4S. 1 hit.
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77223-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAIWIAVAA VSLLGLAFGA ILGYASRRFA VEDDPVVEKI DEILPQSQCG
60 70 80 90 100
QCGYPGCRPY AEAISCNGEK INRCAPGGEA VMLKIAELLN VEPQPLDGEA
110 120 130 140 150
QEITPARMVA VIDENNCIGC TKCIQACPVD AIVGATRAMH TVMSDLCTGC
160 170 180 190
NLCVDPCPTH CISLQPVAET PDSWKWDLNT IPVRIIPVEH HA
Length:192
Mass (Da):20,544
Last modified:February 1, 1997 - v1
Checksum:i69EC54592EACB7B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74700.1.
AP009048 Genomic DNA. Translation: BAA15383.1.
PIRiF64919.
RefSeqiNP_416145.1. NC_000913.3.
YP_489892.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74700; AAC74700; b1628.
BAA15383; BAA15383; BAA15383.
GeneIDi12932727.
946146.
KEGGiecj:Y75_p1605.
eco:b1628.
PATRICi32118558. VBIEscCol129921_1699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74700.1.
AP009048 Genomic DNA. Translation: BAA15383.1.
PIRiF64919.
RefSeqiNP_416145.1. NC_000913.3.
YP_489892.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP77223.
SMRiP77223. Positions 112-162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP77223. 1 interaction.
STRINGi511145.b1628.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74700; AAC74700; b1628.
BAA15383; BAA15383; BAA15383.
GeneIDi12932727.
946146.
KEGGiecj:Y75_p1605.
eco:b1628.
PATRICi32118558. VBIEscCol129921_1699.

Organism-specific databases

EchoBASEiEB3693.
EcoGeneiEG13934. rsxB.

Phylogenomic databases

eggNOGiCOG2878.
HOGENOMiHOG000262938.
InParanoidiP77223.
KOiK03616.
OMAiKVEGDPI.
OrthoDBiEOG60SCM3.
PhylomeDBiP77223.

Enzyme and pathway databases

BioCyciEcoCyc:G6872-MONOMER.
ECOL316407:JW1620-MONOMER.

Miscellaneous databases

PROiP77223.

Gene expression databases

GenevestigatoriP77223.

Family and domain databases

HAMAPiMF_00463. RsxB_RnfB.
InterProiIPR007202. 4Fe-4S_dom.
IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010207. Elect_transpt_cplx_RnfB.
IPR016463. RnfB_Proteobac.
[Graphical view]
PfamiPF00037. Fer4. 2 hits.
PF04060. FeS. 1 hit.
[Graphical view]
PIRSFiPIRSF005784. Elect_transpt_RnfB. 1 hit.
TIGRFAMsiTIGR01944. rnfB. 1 hit.
PROSITEiPS51656. 4FE4S. 1 hit.
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "A reducing system of the superoxide sensor SoxR in Escherichia coli."
    Koo M.S., Lee J.H., Rah S.Y., Yeo W.S., Lee J.W., Lee K.L., Koh Y.S., Kang S.O., Roe J.H.
    EMBO J. 22:2614-2622(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, GENE NAME.

Entry informationi

Entry nameiRSXB_ECOLI
AccessioniPrimary (citable) accession number: P77223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: February 4, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.