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Protein

Putative glutamate--cysteine ligase 2

Gene

ybdK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity. However, because of the low catalytic rate, the question remains whether L-cysteine is the actual biological substrate.UniRule annotation1 Publication

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6326-MONOMER.
ECOL316407:JW0570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutamate--cysteine ligase 2UniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-glutamylcysteine synthetase 2UniRule annotation
Short name:
GCS 2UniRule annotation
Short name:
Gamma-GCS 2UniRule annotation
Gene namesi
Name:ybdK
Ordered Locus Names:b0581, JW0570
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13644. ybdK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002181961 – 372Putative glutamate--cysteine ligase 2Add BLAST372

Proteomic databases

PaxDbiP77213.
PRIDEiP77213.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4260711. 9 interactors.
IntActiP77213. 6 interactors.
STRINGi511145.b0581.

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 24Combined sources11
Turni25 – 28Combined sources4
Helixi35 – 38Combined sources4
Beta strandi41 – 44Combined sources4
Beta strandi46 – 52Combined sources7
Beta strandi54 – 62Combined sources9
Beta strandi65 – 68Combined sources4
Helixi69 – 89Combined sources21
Beta strandi93 – 95Combined sources3
Helixi124 – 127Combined sources4
Beta strandi134 – 140Combined sources7
Helixi144 – 155Combined sources12
Helixi158 – 165Combined sources8
Helixi182 – 186Combined sources5
Helixi201 – 211Combined sources11
Beta strandi214 – 216Combined sources3
Helixi220 – 222Combined sources3
Beta strandi226 – 230Combined sources5
Turni231 – 234Combined sources4
Beta strandi235 – 242Combined sources8
Helixi247 – 267Combined sources21
Helixi274 – 277Combined sources4
Helixi280 – 290Combined sources11
Helixi291 – 293Combined sources3
Beta strandi294 – 297Combined sources4
Turni299 – 301Combined sources3
Beta strandi304 – 306Combined sources3
Helixi307 – 317Combined sources11
Helixi319 – 324Combined sources6
Helixi328 – 340Combined sources13
Helixi344 – 353Combined sources10
Helixi358 – 369Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R8GX-ray2.15A/B1-372[»]
ProteinModelPortaliP77213.
SMRiP77213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77213.

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106KXD. Bacteria.
COG2170. LUCA.
HOGENOMiHOG000220942.
InParanoidiP77213.
KOiK06048.
OMAiAFPMSGR.
PhylomeDBiP77213.

Family and domain databases

HAMAPiMF_01609. Glu_cys_ligase_2. 1 hit.
InterProiIPR006336. GCS2.
IPR011793. YbdK.
[Graphical view]
PfamiPF04107. GCS2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02050. gshA_cyan_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

P77213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPDFHVSE PFTLGIELEM QVVNPPGYDL SQDSSMLIDA VKNKITAGEV
60 70 80 90 100
KHDITESMLE LATDVCRDIN QAAGQFSAMQ KVVLQAATDH HLEICGGGTH
110 120 130 140 150
PFQKWQRQEV CDNERYQRTL ENFGYLIQQA TVFGQHVHVG CASGDDAIYL
160 170 180 190 200
LHGLSRFVPH FIALSAASPY MQGTDTRFAS SRPNIFSAFP DNGPMPWVSN
210 220 230 240 250
WQQFEALFRC LSYTTMIDSI KDLHWDIRPS PHFGTVEVRV MDTPLTLSHA
260 270 280 290 300
VNMAGLIQAT AHWLLTERPF KHQEKDYLLY KFNRFQACRY GLEGVITDPH
310 320 330 340 350
TGDRRPLTED TLRLLEKIAP SAHKIGASSA IEALHRQVVS GLNEAQLMRD
360 370
FVADGGSLIG LVKKHCEIWA GD
Length:372
Mass (Da):41,688
Last modified:February 1, 1997 - v1
Checksum:i898310FD35B8032E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40779.1.
U00096 Genomic DNA. Translation: AAC73682.1.
AP009048 Genomic DNA. Translation: BAA35221.1.
PIRiC64791.
RefSeqiNP_415113.1. NC_000913.3.
WP_001130654.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73682; AAC73682; b0581.
BAA35221; BAA35221; BAA35221.
GeneIDi947246.
KEGGiecj:JW0570.
eco:b0581.
PATRICi32116330. VBIEscCol129921_0605.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40779.1.
U00096 Genomic DNA. Translation: AAC73682.1.
AP009048 Genomic DNA. Translation: BAA35221.1.
PIRiC64791.
RefSeqiNP_415113.1. NC_000913.3.
WP_001130654.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R8GX-ray2.15A/B1-372[»]
ProteinModelPortaliP77213.
SMRiP77213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260711. 9 interactors.
IntActiP77213. 6 interactors.
STRINGi511145.b0581.

Proteomic databases

PaxDbiP77213.
PRIDEiP77213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73682; AAC73682; b0581.
BAA35221; BAA35221; BAA35221.
GeneIDi947246.
KEGGiecj:JW0570.
eco:b0581.
PATRICi32116330. VBIEscCol129921_0605.

Organism-specific databases

EchoBASEiEB3408.
EcoGeneiEG13644. ybdK.

Phylogenomic databases

eggNOGiENOG4106KXD. Bacteria.
COG2170. LUCA.
HOGENOMiHOG000220942.
InParanoidiP77213.
KOiK06048.
OMAiAFPMSGR.
PhylomeDBiP77213.

Enzyme and pathway databases

BioCyciEcoCyc:G6326-MONOMER.
ECOL316407:JW0570-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP77213.
PROiP77213.

Family and domain databases

HAMAPiMF_01609. Glu_cys_ligase_2. 1 hit.
InterProiIPR006336. GCS2.
IPR011793. YbdK.
[Graphical view]
PfamiPF04107. GCS2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02050. gshA_cyan_rel. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGCS2_ECOLI
AccessioniPrimary (citable) accession number: P77213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Catalytic activity was tested towards all 20 common amino acids, but was only observed with L-cysteine.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.