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Protein

Putative glutamate--cysteine ligase 2

Gene

ybdK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity. However, because of the low catalytic rate, the question remains whether L-cysteine is the actual biological substrate.UniRule annotation1 Publication

Miscellaneous

Catalytic activity was tested towards all 20 common amino acids, but was only observed with L-cysteine.

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6326-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutamate--cysteine ligase 2UniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-glutamylcysteine synthetase 2UniRule annotation
Short name:
GCS 2UniRule annotation
Short name:
Gamma-GCS 2UniRule annotation
Gene namesi
Name:ybdK
Ordered Locus Names:b0581, JW0570
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13644 ybdK

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002181961 – 372Putative glutamate--cysteine ligase 2Add BLAST372

Proteomic databases

PaxDbiP77213
PRIDEiP77213

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4260711, 13 interactors
IntActiP77213, 6 interactors
STRINGi316385.ECDH10B_0647

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 24Combined sources11
Turni25 – 28Combined sources4
Helixi35 – 38Combined sources4
Beta strandi41 – 44Combined sources4
Beta strandi46 – 52Combined sources7
Beta strandi54 – 62Combined sources9
Beta strandi65 – 68Combined sources4
Helixi69 – 89Combined sources21
Beta strandi93 – 95Combined sources3
Helixi124 – 127Combined sources4
Beta strandi134 – 140Combined sources7
Helixi144 – 155Combined sources12
Helixi158 – 165Combined sources8
Helixi182 – 186Combined sources5
Helixi201 – 211Combined sources11
Beta strandi214 – 216Combined sources3
Helixi220 – 222Combined sources3
Beta strandi226 – 230Combined sources5
Turni231 – 234Combined sources4
Beta strandi235 – 242Combined sources8
Helixi247 – 267Combined sources21
Helixi274 – 277Combined sources4
Helixi280 – 290Combined sources11
Helixi291 – 293Combined sources3
Beta strandi294 – 297Combined sources4
Turni299 – 301Combined sources3
Beta strandi304 – 306Combined sources3
Helixi307 – 317Combined sources11
Helixi319 – 324Combined sources6
Helixi328 – 340Combined sources13
Helixi344 – 353Combined sources10
Helixi358 – 369Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R8GX-ray2.15A/B1-372[»]
ProteinModelPortaliP77213
SMRiP77213
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77213

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106KXD Bacteria
COG2170 LUCA
HOGENOMiHOG000220942
InParanoidiP77213
KOiK06048
OMAiLDENKWR
PhylomeDBiP77213

Family and domain databases

HAMAPiMF_01609 Glu_cys_ligase_2, 1 hit
InterProiView protein in InterPro
IPR006336 GCS2
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR011793 YbdK
PfamiView protein in Pfam
PF04107 GCS2, 1 hit
SUPFAMiSSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR02050 gshA_cyan_rel, 1 hit

Sequencei

Sequence statusi: Complete.

P77213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPDFHVSE PFTLGIELEM QVVNPPGYDL SQDSSMLIDA VKNKITAGEV
60 70 80 90 100
KHDITESMLE LATDVCRDIN QAAGQFSAMQ KVVLQAATDH HLEICGGGTH
110 120 130 140 150
PFQKWQRQEV CDNERYQRTL ENFGYLIQQA TVFGQHVHVG CASGDDAIYL
160 170 180 190 200
LHGLSRFVPH FIALSAASPY MQGTDTRFAS SRPNIFSAFP DNGPMPWVSN
210 220 230 240 250
WQQFEALFRC LSYTTMIDSI KDLHWDIRPS PHFGTVEVRV MDTPLTLSHA
260 270 280 290 300
VNMAGLIQAT AHWLLTERPF KHQEKDYLLY KFNRFQACRY GLEGVITDPH
310 320 330 340 350
TGDRRPLTED TLRLLEKIAP SAHKIGASSA IEALHRQVVS GLNEAQLMRD
360 370
FVADGGSLIG LVKKHCEIWA GD
Length:372
Mass (Da):41,688
Last modified:February 1, 1997 - v1
Checksum:i898310FD35B8032E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA Translation: AAB40779.1
U00096 Genomic DNA Translation: AAC73682.1
AP009048 Genomic DNA Translation: BAA35221.1
PIRiC64791
RefSeqiNP_415113.1, NC_000913.3
WP_001130654.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73682; AAC73682; b0581
BAA35221; BAA35221; BAA35221
GeneIDi947246
KEGGiecj:JW0570
eco:b0581
PATRICifig|1411691.4.peg.1693

Similar proteinsi

Entry informationi

Entry nameiGCS2_ECOLI
AccessioniPrimary (citable) accession number: P77213
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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