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Protein

Putative glutamate--cysteine ligase 2

Gene

ybdK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity. However, because of the low catalytic rate, the question remains whether L-cysteine is the actual biological substrate.UniRule annotation1 Publication

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6326-MONOMER.
ECOL316407:JW0570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutamate--cysteine ligase 2UniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-glutamylcysteine synthetase 2UniRule annotation
Short name:
GCS 2UniRule annotation
Short name:
Gamma-GCS 2UniRule annotation
Gene namesi
Name:ybdK
Ordered Locus Names:b0581, JW0570
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13644. ybdK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Putative glutamate--cysteine ligase 2PRO_0000218196Add
BLAST

Proteomic databases

PaxDbiP77213.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4260711. 9 interactions.
IntActiP77213. 6 interactions.
STRINGi511145.b0581.

Structurei

Secondary structure

1
372
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 2411Combined sources
Turni25 – 284Combined sources
Helixi35 – 384Combined sources
Beta strandi41 – 444Combined sources
Beta strandi46 – 527Combined sources
Beta strandi54 – 629Combined sources
Beta strandi65 – 684Combined sources
Helixi69 – 8921Combined sources
Beta strandi93 – 953Combined sources
Helixi124 – 1274Combined sources
Beta strandi134 – 1407Combined sources
Helixi144 – 15512Combined sources
Helixi158 – 1658Combined sources
Helixi182 – 1865Combined sources
Helixi201 – 21111Combined sources
Beta strandi214 – 2163Combined sources
Helixi220 – 2223Combined sources
Beta strandi226 – 2305Combined sources
Turni231 – 2344Combined sources
Beta strandi235 – 2428Combined sources
Helixi247 – 26721Combined sources
Helixi274 – 2774Combined sources
Helixi280 – 29011Combined sources
Helixi291 – 2933Combined sources
Beta strandi294 – 2974Combined sources
Turni299 – 3013Combined sources
Beta strandi304 – 3063Combined sources
Helixi307 – 31711Combined sources
Helixi319 – 3246Combined sources
Helixi328 – 34013Combined sources
Helixi344 – 35310Combined sources
Helixi358 – 36912Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R8GX-ray2.15A/B1-372[»]
ProteinModelPortaliP77213.
SMRiP77213. Positions 2-370.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77213.

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106KXD. Bacteria.
COG2170. LUCA.
HOGENOMiHOG000220942.
InParanoidiP77213.
KOiK06048.
OMAiAFPMSGR.
PhylomeDBiP77213.

Family and domain databases

HAMAPiMF_01609. Glu_cys_ligase_2. 1 hit.
InterProiIPR006336. GCS2.
IPR011793. YbdK.
[Graphical view]
PfamiPF04107. GCS2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02050. gshA_cyan_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

P77213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPDFHVSE PFTLGIELEM QVVNPPGYDL SQDSSMLIDA VKNKITAGEV
60 70 80 90 100
KHDITESMLE LATDVCRDIN QAAGQFSAMQ KVVLQAATDH HLEICGGGTH
110 120 130 140 150
PFQKWQRQEV CDNERYQRTL ENFGYLIQQA TVFGQHVHVG CASGDDAIYL
160 170 180 190 200
LHGLSRFVPH FIALSAASPY MQGTDTRFAS SRPNIFSAFP DNGPMPWVSN
210 220 230 240 250
WQQFEALFRC LSYTTMIDSI KDLHWDIRPS PHFGTVEVRV MDTPLTLSHA
260 270 280 290 300
VNMAGLIQAT AHWLLTERPF KHQEKDYLLY KFNRFQACRY GLEGVITDPH
310 320 330 340 350
TGDRRPLTED TLRLLEKIAP SAHKIGASSA IEALHRQVVS GLNEAQLMRD
360 370
FVADGGSLIG LVKKHCEIWA GD
Length:372
Mass (Da):41,688
Last modified:February 1, 1997 - v1
Checksum:i898310FD35B8032E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40779.1.
U00096 Genomic DNA. Translation: AAC73682.1.
AP009048 Genomic DNA. Translation: BAA35221.1.
PIRiC64791.
RefSeqiNP_415113.1. NC_000913.3.
WP_001130654.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73682; AAC73682; b0581.
BAA35221; BAA35221; BAA35221.
GeneIDi947246.
KEGGiecj:JW0570.
eco:b0581.
PATRICi32116330. VBIEscCol129921_0605.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40779.1.
U00096 Genomic DNA. Translation: AAC73682.1.
AP009048 Genomic DNA. Translation: BAA35221.1.
PIRiC64791.
RefSeqiNP_415113.1. NC_000913.3.
WP_001130654.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R8GX-ray2.15A/B1-372[»]
ProteinModelPortaliP77213.
SMRiP77213. Positions 2-370.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260711. 9 interactions.
IntActiP77213. 6 interactions.
STRINGi511145.b0581.

Proteomic databases

PaxDbiP77213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73682; AAC73682; b0581.
BAA35221; BAA35221; BAA35221.
GeneIDi947246.
KEGGiecj:JW0570.
eco:b0581.
PATRICi32116330. VBIEscCol129921_0605.

Organism-specific databases

EchoBASEiEB3408.
EcoGeneiEG13644. ybdK.

Phylogenomic databases

eggNOGiENOG4106KXD. Bacteria.
COG2170. LUCA.
HOGENOMiHOG000220942.
InParanoidiP77213.
KOiK06048.
OMAiAFPMSGR.
PhylomeDBiP77213.

Enzyme and pathway databases

BioCyciEcoCyc:G6326-MONOMER.
ECOL316407:JW0570-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP77213.
PROiP77213.

Family and domain databases

HAMAPiMF_01609. Glu_cys_ligase_2. 1 hit.
InterProiIPR006336. GCS2.
IPR011793. YbdK.
[Graphical view]
PfamiPF04107. GCS2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02050. gshA_cyan_rel. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGCS2_ECOLI
AccessioniPrimary (citable) accession number: P77213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Catalytic activity was tested towards all 20 common amino acids, but was only observed with L-cysteine.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.