Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable pyridine nucleotide-disulfide oxidoreductase RclA

Gene

rclA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probably involved in reactive chlorine species (RCS) stress resistance.1 Publication

Miscellaneous

The active site is a redox-active disulfide bond.By similarity

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei426Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 43FADBy similarityAdd BLAST11

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: GO_Central
  • cellular oxidant detoxification Source: GO_Central
  • response to hypochlorite Source: EcoCyc
  • response to oxygen radical Source: GO_Central

Keywordsi

Molecular functionOxidoreductase
Biological processStress response
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6174-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pyridine nucleotide-disulfide oxidoreductase RclA
Alternative name(s):
Reactive chlorine resistance protein A
Gene namesi
Name:rclA
Synonyms:ykgC
Ordered Locus Names:b0304, JW5040
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG13580 rclA

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Mutants are more sensitive to HOCl treatment than wild-type cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000680591 – 441Probable pyridine nucleotide-disulfide oxidoreductase RclAAdd BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 48Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP77212
PRIDEiP77212

Expressioni

Inductioni

Induced by RclR in response to hypochlorous acid (HOCl).1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259800, 12 interactors
DIPiDIP-12675N
IntActiP77212, 2 interactors
STRINGi316385.ECDH10B_0292

Structurei

3D structure databases

ProteinModelPortaliP77212
SMRiP77212
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4107QN2 Bacteria
COG1249 LUCA
HOGENOMiHOG000276709
InParanoidiP77212
KOiK21739
OMAiTMSEAFN
PhylomeDBiP77212

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR001100 Pyr_nuc-diS_OxRdtase
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
IPR012999 Pyr_OxRdtase_I_AS
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
PIRSFiPIRSF000350 Mercury_reductase_MerA, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit
PROSITEiView protein in PROSITE
PS00076 PYRIDINE_REDOX_1, 1 hit

Sequencei

Sequence statusi: Complete.

P77212-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKYQAVIIG FGKAGKTLAV TLAKAGWRVA LIEQSNAMYG GTCINIGCIP
60 70 80 90 100
TKTLVHDAQQ HTDFVRAIQR KNEVVNFLRN KNFHNLADMP NIDVIDGQAE
110 120 130 140 150
FINNHSLRVH RPEGNLEIHG EKIFINTGAQ TVVPPIPGIT TTPGVYDSTG
160 170 180 190 200
LLNLKELPGH LGILGGGYIG VEFASMFANF GSKVTILEAA SLFLPREDRD
210 220 230 240 250
IADNIATILR DQGVDIILNA HVERISHHEN QVQVHSEHAQ LAVDALLIAS
260 270 280 290 300
GRQPATASLH PENAGIAVNE RGAIVVDKRL HTTADNIWAM GDVTGGLQFT
310 320 330 340 350
YISLDDYRIV RDELLGEGKR STDDRKNVPY SVFMTPPLSR VGMTEEQARE
360 370 380 390 400
SGADIQVVTL PVAAIPRARV MNDTRGVLKA IVDNKTQRML GASLLCVDSH
410 420 430 440
EMINIVKMVM DAGLPYSILR DQIFTHPSMS ESLNDLFSLV K
Length:441
Mass (Da):48,244
Last modified:August 29, 2001 - v2
Checksum:i53B9C0B52C7DA8A0
GO

Sequence cautioni

The sequence AAB18031 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA Translation: AAB18031.1 Different initiation.
U00096 Genomic DNA Translation: AAC73407.2
AP009048 Genomic DNA Translation: BAE76088.1
PIRiH64756
RefSeqiNP_414838.2, NC_000913.3
WP_001046307.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73407; AAC73407; b0304
BAE76088; BAE76088; BAE76088
GeneIDi946092
KEGGiecj:JW5040
eco:b0304
PATRICifig|511145.12.peg.311

Similar proteinsi

Entry informationi

Entry nameiRCLA_ECOLI
AccessioniPrimary (citable) accession number: P77212
Secondary accession number(s): Q2MCB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 29, 2001
Last modified: March 28, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health