Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cation efflux system protein CusC

Gene

cusC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms pores that allow passive diffusion of cations across the outer membrane. Part of a cation efflux system that mediates resistance to copper and silver. In pathogenic strains it allows the bacteria to invade brain microvascular endothelial cells (BMEC) thus allowing it to cross the blood-brain barrier and cause neonatal meningitis.3 Publications

GO - Molecular functioni

  • copper ion binding Source: EcoCyc
  • copper ion transmembrane transporter activity Source: EcoCyc
  • diacylglycerol binding Source: UniProtKB
  • porin activity Source: UniProtKB-KW

GO - Biological processi

  • cellular copper ion homeostasis Source: EcoCyc
  • copper ion export Source: EcoCyc
  • detoxification of copper ion Source: EcoCyc
  • protein homotrimerization Source: UniProtKB
  • protein palmitoylation Source: UniProtKB
  • response to copper ion Source: EcoliWiki
  • response to silver ion Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6320-MONOMER.
ECOL316407:JW0561-MONOMER.
MetaCyc:G6320-MONOMER.

Protein family/group databases

TCDBi1.B.17.3.5. the outer membrane factor (omf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cation efflux system protein CusC
Gene namesi
Name:cusC
Synonyms:ibeB, ylcB
Ordered Locus Names:b0572, JW0561
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14233. cusC.

Subcellular locationi

  • Cell outer membrane 1 Publication; Multi-pass membrane protein 1 Publication
  • Cell outer membrane 1 Publication; Lipid-anchor PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717PROSITE-ProRule annotationAdd
BLAST
Chaini18 – 457440Cation efflux system protein CusCPRO_0000030992Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi18 – 181N-palmitoyl cysteinePROSITE-ProRule annotation1 Publication
Lipidationi18 – 181S-diacylglycerol cysteinePROSITE-ProRule annotation1 Publication

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP77211.
PRIDEiP77211.

Expressioni

Inductioni

Transcriptionally regulated by CusR in response to copper and silver ions.1 Publication

Interactioni

Subunit structurei

Homotrimer. Component of the cus efflux system composed of CusA, CusB, CusC and CusF.1 Publication

Protein-protein interaction databases

BioGridi4262822. 260 interactions.
DIPiDIP-9347N.
STRINGi511145.b0572.

Structurei

Secondary structure

1
457
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 353Combined sources
Helixi54 – 574Combined sources
Helixi61 – 7313Combined sources
Helixi75 – 9622Combined sources
Beta strandi101 – 11111Combined sources
Beta strandi114 – 1174Combined sources
Beta strandi121 – 13313Combined sources
Helixi139 – 20466Combined sources
Turni205 – 2073Combined sources
Helixi211 – 24636Combined sources
Helixi260 – 2623Combined sources
Helixi274 – 2796Combined sources
Helixi281 – 30222Combined sources
Beta strandi307 – 32014Combined sources
Helixi321 – 3233Combined sources
Helixi327 – 3293Combined sources
Beta strandi330 – 33910Combined sources
Helixi347 – 41367Combined sources
Helixi419 – 45335Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PIKX-ray2.30A18-457[»]
4K34X-ray2.69A/B18-457[»]
4K7KX-ray2.53A/B18-457[»]
4K7RX-ray2.09A18-457[»]
ProteinModelPortaliP77211.
SMRiP77211. Positions 18-457.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77211.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105ENJ. Bacteria.
COG1538. LUCA.
HOGENOMiHOG000111955.
InParanoidiP77211.
KOiK07796.
OMAiMIESTRA.
PhylomeDBiP77211.

Family and domain databases

InterProiIPR003423. OMP_efflux.
IPR010131. RND_efflux_OM_lipoprot_NodT.
[Graphical view]
PfamiPF02321. OEP. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01845. outer_NodT. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPCKLLPFC VALALTGCSL APDYQRPAMP VPQQFSLSQN GLVNAADNYQ
60 70 80 90 100
NAGWRTFFVD NQVKTLISEA LVNNRDLRMA TLKVQEARAQ YRLTDADRYP
110 120 130 140 150
QLNGEGSGSW SGNLKGNTAT TREFSTGLNA SFDLDFFGRL KNMSEAERQN
160 170 180 190 200
YLATEEAQRA VHILLVSNVA QSYFNQQLAY AQLQIAEETL RNYQQSYAFV
210 220 230 240 250
EKQLLTGSSN VLALEQARGV IESTRSDIAK RQGELAQANN ALQLLLGSYG
260 270 280 290 300
KLPQAQTVNS DSLQSVKLPA GLSSQILLQR PDIMEAEHAL MAANANIGAA
310 320 330 340 350
RAAFFPSISL TSGISTASSD LSSLFNASSG MWNFIPKIEI PIFNAGRNQA
360 370 380 390 400
NLDIAEIRQQ QSVVNYEQKI QNAFKEVADA LALRQSLNDQ ISAQQRYLAS
410 420 430 440 450
LQITLQRARA LYQHGAVSYL EVLDAERSLF ATRQTLLDLN YARQVNEISL

YTALGGG
Length:457
Mass (Da):50,270
Last modified:February 1, 1997 - v1
Checksum:iB85B2B6B6719F7A1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti6 – 61L → I in strain: K1 / RS218.
Natural varianti20 – 201L → M in strain: K1 / RS218.
Natural varianti27 – 271P → S in strain: K1 / RS218.
Natural varianti33 – 331Q → H in strain: K1 / RS218.
Natural varianti50 – 512QN → RS in strain: K1 / RS218.
Natural varianti81 – 811T → A in strain: K1 / RS218.
Natural varianti102 – 1021L → F in strain: K1 / RS218.
Natural varianti106 – 1061G → D in strain: K1 / RS218.
Natural varianti117 – 1182NT → DS in strain: K1 / RS218.
Natural varianti167 – 1671S → F in strain: K1 / RS218.
Natural varianti191 – 1911R → G in strain: K1 / RS218.
Natural varianti232 – 2321Q → R in strain: K1 / RS218.
Natural varianti273 – 2731S → P in strain: K1 / RS218.
Natural varianti457 – 4571G → GWQQ in strain: K1 / RS218.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF094824 Genomic DNA. Translation: AAD30205.1.
U82598 Genomic DNA. Translation: AAB40770.1.
U00096 Genomic DNA. Translation: AAC73673.1.
AP009048 Genomic DNA. Translation: BAA35206.1.
AF245661 Genomic DNA. Translation: AAF70174.1.
PIRiB64790.
RefSeqiNP_415104.1. NC_000913.3.
WP_000074234.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73673; AAC73673; b0572.
BAA35206; BAA35206; BAA35206.
GeneIDi946288.
KEGGiecj:JW0561.
eco:b0572.
PATRICi32116312. VBIEscCol129921_0596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF094824 Genomic DNA. Translation: AAD30205.1.
U82598 Genomic DNA. Translation: AAB40770.1.
U00096 Genomic DNA. Translation: AAC73673.1.
AP009048 Genomic DNA. Translation: BAA35206.1.
AF245661 Genomic DNA. Translation: AAF70174.1.
PIRiB64790.
RefSeqiNP_415104.1. NC_000913.3.
WP_000074234.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PIKX-ray2.30A18-457[»]
4K34X-ray2.69A/B18-457[»]
4K7KX-ray2.53A/B18-457[»]
4K7RX-ray2.09A18-457[»]
ProteinModelPortaliP77211.
SMRiP77211. Positions 18-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262822. 260 interactions.
DIPiDIP-9347N.
STRINGi511145.b0572.

Protein family/group databases

TCDBi1.B.17.3.5. the outer membrane factor (omf) family.

Proteomic databases

PaxDbiP77211.
PRIDEiP77211.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73673; AAC73673; b0572.
BAA35206; BAA35206; BAA35206.
GeneIDi946288.
KEGGiecj:JW0561.
eco:b0572.
PATRICi32116312. VBIEscCol129921_0596.

Organism-specific databases

EchoBASEiEB3984.
EcoGeneiEG14233. cusC.

Phylogenomic databases

eggNOGiENOG4105ENJ. Bacteria.
COG1538. LUCA.
HOGENOMiHOG000111955.
InParanoidiP77211.
KOiK07796.
OMAiMIESTRA.
PhylomeDBiP77211.

Enzyme and pathway databases

BioCyciEcoCyc:G6320-MONOMER.
ECOL316407:JW0561-MONOMER.
MetaCyc:G6320-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP77211.
PROiP77211.

Family and domain databases

InterProiIPR003423. OMP_efflux.
IPR010131. RND_efflux_OM_lipoprot_NodT.
[Graphical view]
PfamiPF02321. OEP. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01845. outer_NodT. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUSC_ECOLI
AccessioniPrimary (citable) accession number: P77211
Secondary accession number(s): Q9L5Y3, Q9X444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.