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Protein

Putative outer membrane usher protein YfcU

Gene

yfcU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein uncertaini

Functioni

Part of the yfcOPQRSUV fimbrial operon. Could contribute to adhesion to various surfaces in specific environmental niches. Increases adhesion to eukaryotic T24 bladder epithelial cells in the absence of fim genes. Probably involved in the export and assembly of fimbrial subunits across the outer membrane.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fimbrium biogenesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7208-MONOMER.
EcoCyc:G7209-MONOMER.
ECOL316407:JW2334-MONOMER.
ECOL316407:JW2335-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative outer membrane usher protein YfcU
Gene namesi
Name:yfcU
Synonyms:yfcT
Ordered Locus Names:b4661, JW2334/JW2335
ORF Names:b2337, b2338
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14124. yfcU.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the operon under classical laboratory conditions does not result in any major effect on E.coli capacity to form biofilms compared with the wild-type strain.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 881852Putative outer membrane usher protein YfcUPRO_0000009332Add
BLAST

Proteomic databases

PRIDEiP77196.

Expressioni

Inductioni

Expression is negatively regulated by H-NS and subjected to cAMP receptor protein (CRP)-mediated catabolite repression.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261776. 11 interactions.
4262083. 222 interactions.
DIPiDIP-11989N.
IntActiP77196. 20 interactions.
STRINGi316407.1799729.

Structurei

3D structure databases

ProteinModelPortaliP77196.
SMRiP77196. Positions 172-670, 764-843.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fimbrial export usher family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4106PG1. Bacteria.
COG3188. LUCA.
HOGENOMiHOG000260138.
InParanoidiP77196.
OMAiTQCEAAL.
PhylomeDBiP77196.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDHSLFRLR ILPWCIALAM SGSYSSVWAE DDIQFDSRFL ELKGDTKIDL
60 70 80 90 100
KRFSSQGYVE PGKYNLQVQL NKQPLAEEYD IYWYAGEDDV SKSYACLTPE
110 120 130 140 150
LVAQFGLKED VAKNLQWSHD GKCLKPGQLE GVEIKADLSQ SALVISLPQA
160 170 180 190 200
YLEYTWPDWD PPSRWDDGIS GIIADYSITA QTRHEENGGD DSNEISGNGT
210 220 230 240 250
VGVNLGPWRM RADWQTNYQH TRSNDDDDEF GGDDTQKKWE WSRYYAWRAL
260 270 280 290 300
PSLKAKLALG EDYLNSDIFD GFNYVGGSVS TDDQMLPPNL RGYAPDISGV
310 320 330 340 350
AHTTAKVTVS QMGRVIYETQ VPAGPFRIQD LGDSVSGTLH IRIEEQNGQV
360 370 380 390 400
QEYDISTASM PYLTRPGQVR YKIMMGRPQE WGHHVEGGFF SGAEASWGIA
410 420 430 440 450
NGWSLYGGAL GDENYQSAAL GVGRDLSTFG AVAFDVTHSH TKLDKDTAYG
460 470 480 490 500
KGSLDGNSFR VSYSKDFDQL NSRVTFAGYR FSEENFMTMS EYLDASDSEM
510 520 530 540 550
VRTGNDKEMY TATYNQNFRD AGVSVYLNYT RHTYWDREEQ TNYNIMLSHY
560 570 580 590 600
FNMGSIRNMS VSLTGYRYEY DNRADKGMYI SLSMPWGDNS TVSYNGNYGS
610 620 630 640 650
GTDSSQVGYF SRVDDATHYQ LNIGTSDKHT SVDGYYSHDG SLAQVDLSAN
660 670 680 690 700
YHEGQYTSAG LSLQGGATLT THGGALHRTQ NMGGTRLLID ADGVADVPVE
710 720 730 740 750
GNGAAVYTNM FGKAVVSDVN NYYRNQAYID LNKLPENAEA TQSVVQATLT
760 770 780 790 800
EGAIGYRKFA VISGQKAMAV LRLQDGSHPP FGAEVKNDNE QTVGLVDDDG
810 820 830 840 850
SVYLAGVKPG EHMSVFWSGV AHCDINLPDP LPADLFNGLL LPCQHKGNVA
860 870 880
PVVPDDIKPV IQEQTQQVTP TDPPVSVSAN Q
Length:881
Mass (Da):97,490
Last modified:January 9, 2007 - v3
Checksum:i48B392C1AE2C9111
GO

Sequence cautioni

The sequence BAA16191.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA16192.1 differs from that shown. Reason: Erroneous termination at position 579. Translated as Tyr.Curated
The sequence U00096 differs from that shown. Reason: Erroneous termination at position 579. Translated as Tyr.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. No translation available.
AP009048 Genomic DNA. Translation: BAA16191.1. Different initiation.
AP009048 Genomic DNA. Translation: BAA16192.1. Sequence problems.
PIRiG65006.
H65006.

Genome annotation databases

EnsemblBacteriaiBAA16191; BAA16191; BAA16191.
BAA16192; BAA16192; BAA16192.
KEGGiecj:JW2334.
ecj:JW2335.
PATRICi48664076. VBIEscCol107702_2379.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. No translation available.
AP009048 Genomic DNA. Translation: BAA16191.1. Different initiation.
AP009048 Genomic DNA. Translation: BAA16192.1. Sequence problems.
PIRiG65006.
H65006.

3D structure databases

ProteinModelPortaliP77196.
SMRiP77196. Positions 172-670, 764-843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261776. 11 interactions.
4262083. 222 interactions.
DIPiDIP-11989N.
IntActiP77196. 20 interactions.
STRINGi316407.1799729.

Proteomic databases

PRIDEiP77196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA16191; BAA16191; BAA16191.
BAA16192; BAA16192; BAA16192.
KEGGiecj:JW2334.
ecj:JW2335.
PATRICi48664076. VBIEscCol107702_2379.

Organism-specific databases

EchoBASEiEB3876.
EcoGeneiEG14124. yfcU.

Phylogenomic databases

eggNOGiENOG4106PG1. Bacteria.
COG3188. LUCA.
HOGENOMiHOG000260138.
InParanoidiP77196.
OMAiTQCEAAL.
PhylomeDBiP77196.

Enzyme and pathway databases

BioCyciEcoCyc:G7208-MONOMER.
EcoCyc:G7209-MONOMER.
ECOL316407:JW2334-MONOMER.
ECOL316407:JW2335-MONOMER.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities."
    Korea C.G., Badouraly R., Prevost M.C., Ghigo J.M., Beloin C.
    Environ. Microbiol. 12:1957-1977(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiYFCU_ECOLI
AccessioniPrimary (citable) accession number: P77196
Secondary accession number(s): P77532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 9, 2007
Last modified: July 6, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The operon is cryptic under classical laboratory conditions, but is functional when constitutively expressed.1 Publication

Caution

Could be the product of a pseudogene. This sequence is interrupted by a stop codon.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.