Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cell division protein ZipA

Gene

zipA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential cell division protein that stabilizes the FtsZ protofilaments by cross-linking them and that serves as a cytoplasmic membrane anchor for the Z ring (PubMed:9008158, PubMed:11847116, PubMed:22164258, PubMed:22304478, PubMed:23233671). Also required for the recruitment to the septal ring of the downstream cell division proteins FtsK, FtsQ, FtsL and FtsN (PubMed:11847116, PubMed:11948172). ZipA overproduction protects FtsZ from degradation by ClpP by preventing recognition by ClpX (PubMed:23233671). Does not affect the GTPase activity of FtsZ (PubMed:10209756).7 Publications

GO - Biological processi

  • barrier septum assembly Source: EcoliWiki
  • FtsZ-dependent cytokinesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:G7258-MONOMER.
ECOL316407:JW2404-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division protein ZipAUniRule annotation
Alternative name(s):
FtsZ interacting protein A1 Publication
Gene namesi
Name:zipA1 PublicationUniRule annotation
Ordered Locus Names:b2412, JW2404
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14169. zipA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6PeriplasmicUniRule annotation1 Publication6
Transmembranei7 – 27HelicalUniRule annotationAdd BLAST21
Topological domaini28 – 328CytoplasmicUniRule annotation1 PublicationAdd BLAST301

GO - Cellular componenti

  • cell division site Source: EcoliWiki
  • integral component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3954.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002145211 – 328Cell division protein ZipAAdd BLAST328

Proteomic databases

PaxDbiP77173.
PRIDEiP77173.

Expressioni

Inductioni

Repressed 1.5-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Interacts with FtsZ via their C-terminal domains (PubMed:9008158, PubMed:10209756, PubMed:10880432, PubMed:22164258). Can form homodimers prior to association with FtsZ (PubMed:22304478).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ftsZP0A9A64EBI-1029213,EBI-370963

Protein-protein interaction databases

BioGridi4261708. 552 interactors.
DIPiDIP-1157N.
IntActiP77173. 2 interactors.
MINTiMINT-89938.
STRINGi511145.b2412.

Chemistry databases

BindingDBiP77173.

Structurei

Secondary structure

1328
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi193 – 200Combined sources8
Helixi209 – 218Combined sources10
Beta strandi221 – 223Combined sources3
Helixi225 – 227Combined sources3
Beta strandi228 – 234Combined sources7
Beta strandi241 – 249Combined sources9
Turni256 – 259Combined sources4
Beta strandi262 – 272Combined sources11
Beta strandi274 – 276Combined sources3
Helixi278 – 296Combined sources19
Beta strandi299 – 301Combined sources3
Beta strandi305 – 307Combined sources3
Helixi310 – 326Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F46X-ray1.50A/B189-328[»]
1F47X-ray1.95B185-328[»]
1F7WNMR-A185-328[»]
1F7XNMR-A185-328[»]
1S1JX-ray2.18A/B185-328[»]
1S1SX-ray2.10A/B185-328[»]
1Y2FX-ray2.00A190-328[»]
1Y2GX-ray1.90A/B189-328[»]
DisProtiDP00161.
ProteinModelPortaliP77173.
SMRiP77173.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77173.

Family & Domainsi

Domaini

Contains an N-terminal transmembrane domain, followed by a charged domain, an unstructured P/Q domain rich in proline and glutamine, and a C-terminal FtsZ-binding domain.1 Publication

Sequence similaritiesi

Belongs to the ZipA family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107NPY. Bacteria.
COG3115. LUCA.
HOGENOMiHOG000274007.
InParanoidiP77173.
KOiK03528.
OMAiLPCYGEA.

Family and domain databases

CDDicd00231. ZipA. 1 hit.
Gene3Di3.30.1400.10. 1 hit.
HAMAPiMF_00509. ZipA. 1 hit.
InterProiIPR011919. Cell_div_ZipA.
IPR007449. ZipA_FtsZ-bd_C.
[Graphical view]
PfamiPF04354. ZipA_C. 1 hit.
[Graphical view]
ProDomiPD035754. ZipA_Fts_Z_bd_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00771. ZipA_C. 1 hit.
[Graphical view]
SUPFAMiSSF64383. SSF64383. 1 hit.
TIGRFAMsiTIGR02205. septum_zipA. 1 hit.

Sequencei

Sequence statusi: Complete.

P77173-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQDLRLILI IVGAIAIIAL LVHGFWTSRK ERSSMFRDRP LKRMKSKRDD
60 70 80 90 100
DSYDEDVEDD EGVGEVRVHR VNHAPANAQE HEAARPSPQH QYQPPYASAQ
110 120 130 140 150
PRQPVQQPPE AQVPPQHAPH PAQPVQQPAY QPQPEQPLQQ PVSPQVAPAP
160 170 180 190 200
QPVHSAPQPA QQAFQPAEPV AAPQPEPVAE PAPVMDKPKR KEAVIIMNVA
210 220 230 240 250
AHHGSELNGE LLLNSIQQAG FIFGDMNIYH RHLSPDGSGP ALFSLANMVK
260 270 280 290 300
PGTFDPEMKD FTTPGVTIFM QVPSYGDELQ NFKLMLQSAQ HIADEVGGVV
310 320
LDDQRRMMTP QKLREYQDII REVKDANA
Length:328
Mass (Da):36,475
Last modified:August 29, 2003 - v3
Checksum:iA20F119A153A782B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211L → A in AAB42061 (PubMed:9008158).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U74650 Genomic DNA. Translation: AAB42061.1.
U00096 Genomic DNA. Translation: AAC75465.1.
AP009048 Genomic DNA. Translation: BAA16284.1.
PIRiC65015.
RefSeqiNP_416907.1. NC_000913.3.
WP_001300494.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75465; AAC75465; b2412.
BAA16284; BAA16284; BAA16284.
GeneIDi946869.
KEGGiecj:JW2404.
eco:b2412.
PATRICi32120207. VBIEscCol129921_2506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U74650 Genomic DNA. Translation: AAB42061.1.
U00096 Genomic DNA. Translation: AAC75465.1.
AP009048 Genomic DNA. Translation: BAA16284.1.
PIRiC65015.
RefSeqiNP_416907.1. NC_000913.3.
WP_001300494.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F46X-ray1.50A/B189-328[»]
1F47X-ray1.95B185-328[»]
1F7WNMR-A185-328[»]
1F7XNMR-A185-328[»]
1S1JX-ray2.18A/B185-328[»]
1S1SX-ray2.10A/B185-328[»]
1Y2FX-ray2.00A190-328[»]
1Y2GX-ray1.90A/B189-328[»]
DisProtiDP00161.
ProteinModelPortaliP77173.
SMRiP77173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261708. 552 interactors.
DIPiDIP-1157N.
IntActiP77173. 2 interactors.
MINTiMINT-89938.
STRINGi511145.b2412.

Chemistry databases

BindingDBiP77173.
ChEMBLiCHEMBL3954.

Proteomic databases

PaxDbiP77173.
PRIDEiP77173.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75465; AAC75465; b2412.
BAA16284; BAA16284; BAA16284.
GeneIDi946869.
KEGGiecj:JW2404.
eco:b2412.
PATRICi32120207. VBIEscCol129921_2506.

Organism-specific databases

EchoBASEiEB3921.
EcoGeneiEG14169. zipA.

Phylogenomic databases

eggNOGiENOG4107NPY. Bacteria.
COG3115. LUCA.
HOGENOMiHOG000274007.
InParanoidiP77173.
KOiK03528.
OMAiLPCYGEA.

Enzyme and pathway databases

BioCyciEcoCyc:G7258-MONOMER.
ECOL316407:JW2404-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP77173.
PROiP77173.

Family and domain databases

CDDicd00231. ZipA. 1 hit.
Gene3Di3.30.1400.10. 1 hit.
HAMAPiMF_00509. ZipA. 1 hit.
InterProiIPR011919. Cell_div_ZipA.
IPR007449. ZipA_FtsZ-bd_C.
[Graphical view]
PfamiPF04354. ZipA_C. 1 hit.
[Graphical view]
ProDomiPD035754. ZipA_Fts_Z_bd_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00771. ZipA_C. 1 hit.
[Graphical view]
SUPFAMiSSF64383. SSF64383. 1 hit.
TIGRFAMsiTIGR02205. septum_zipA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiZIPA_ECOLI
AccessioniPrimary (citable) accession number: P77173
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 29, 2003
Last modified: November 30, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Has been isolated as a 91 kDa complex containing ZipA-EptA and an unidentified 24 kDa protein (PubMed:16079137), but it was shown later that there is no physical interaction between ZipA and EptA (PubMed:22304478).2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.