Putative xanthine dehydrogenase YagT iron-sulfur-binding subunit precursor

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P77165 (YAGT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative xanthine dehydrogenase YagT iron-sulfur-binding subunit

Gene names

Name:	yagT
Ordered Locus Names:	b0286, JW0280

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Iron-sulfur subunit of the xanthine dehydrogenase complex By similarity.
Subunit structure	Heterotrimer of YagR, YagS and YagT Potential.
Post-translational modification	Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Purine metabolism Purine salvage
Domain	Signal
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	periplasmic space Inferred from direct assay Ref.4. Source: EcoCyc
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from direct assay PubMed 19368556. Source: EcoCyc electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on the aldehyde or oxo group of donors Inferred from direct assay PubMed 19368556. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 53

Tat-type signal Potential

Chain

54 – 229

176

Putative xanthine dehydrogenase YagT iron-sulfur-binding subunit

PRO_0000189414

Regions

Domain

61 – 137

2Fe-2S ferredoxin-type

Sites

Metal binding

Iron-sulfur (2Fe-2S) By similarity

Metal binding

104

Iron-sulfur (2Fe-2S) By similarity

Metal binding

107

Iron-sulfur (2Fe-2S) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P77165 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 216961D7BD21836C
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FASTA

229

24,343

        10         20         30         40         50         60 
MSNQGEYPED NRVGKHEPHD LSLTRRDLIK VSAATAATAV VYPHSTLAAS VPAATPAPEI 

        70         80         90        100        110        120 
MPLTLKVNGK TEQLEVDTRT TLLDTLRENL HLIGTKKGCD HGQCGACTVL VNGRRLNACL 

       130        140        150        160        170        180 
TLAVMHQGAE ITTIEGLGSP DNLHPMQAAF IKHDGFQCGY CTSGQICSSV AVLKEIQDGI 

       190        200        210        220 
PSHVTVDLVS APETTADEIR ERMSGNICRC GAYANILAAI EDAAGEIKS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G. J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract] Cited for: EXPORT VIA THE TAT-SYSTEM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73857 Genomic DNA. Translation: AAB18015.1. U00096 Genomic DNA. Translation: AAC73389.1. AP009048 Genomic DNA. Translation: BAE76070.1.
PIR	F64754.
RefSeq	NP_414820.1. NC_000913.2. YP_488582.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P77165.
SMR	P77165. Positions 63-225.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77165. 8 interactions.
STRING	511145.b0286.
Proteomic databases
PRIDE	P77165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73389; AAC73389; b0286. BAE76070; BAE76070; BAE76070.
GeneID	12930792. 945330.
KEGG	ecj:Y75_p0278. eco:b0286.
PATRIC	32115695. VBIEscCol129921_0290.
Organism-specific databases
EchoBASE	EB3329.
EcoGene	EG13559. yagT.
Phylogenomic databases
eggNOG	COG2080.
HOGENOM	HOG000166647.
KO	K13483.
OMA	HTSHIDL.
ProtClustDB	PRK11433.
Enzyme and pathway databases
BioCyc	EcoCyc:G6157-MONOMER. ECOL316407:JW0280-MONOMER. MetaCyc:G6157-MONOMER.
Gene expression databases
Genevestigator	P77165.
Family and domain databases
Gene3D	1.10.150.120. 1 hit. 3.10.20.30. 1 hit.
InterPro	IPR002888. 2Fe-2S-bd. IPR001041. 2Fe-2S_ferredoxin-type. IPR006058. 2Fe2S_fd_BS. IPR012675. Beta-grasp_dom. IPR006311. TAT_signal. [Graphical view]
Pfam	PF00111. Fer2. 1 hit. PF01799. Fer2_2. 1 hit. [Graphical view]
SUPFAM	SSF47741. 2Fe-2S_bind. 1 hit. SSF54292. Ferredoxin. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAGT_ECOLI

Accession

Primary (citable) accession number: P77165
Secondary accession number(s): Q2MCD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents