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Protein

Putative xanthine dehydrogenase YagT iron-sulfur-binding subunit

Gene

yagT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Iron-sulfur subunit of the xanthine dehydrogenase complex.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi104 – 1041Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi107 – 1071Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • electron carrier activity Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • oxidoreductase activity, acting on the aldehyde or oxo group of donors Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism, Purine salvage

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6157-MONOMER.
ECOL316407:JW0280-MONOMER.
MetaCyc:G6157-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative xanthine dehydrogenase YagT iron-sulfur-binding subunit
Gene namesi
Name:yagT
Ordered Locus Names:b0286, JW0280
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13559. yagT.

Subcellular locationi

GO - Cellular componenti

  • periplasmic space Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5353Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini54 – 229176Putative xanthine dehydrogenase YagT iron-sulfur-binding subunitPRO_0000189414Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PaxDbiP77165.
PRIDEiP77165.

Interactioni

Subunit structurei

Heterotrimer of YagR, YagS and YagT.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
proXP0AFM22EBI-1115563,EBI-1129961

Protein-protein interaction databases

BioGridi4259786. 3 interactions.
IntActiP77165. 10 interactions.
STRINGi511145.b0286.

Structurei

3D structure databases

ProteinModelPortaliP77165.
SMRiP77165. Positions 63-225.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 137772Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107XKX. Bacteria.
COG2080. LUCA.
HOGENOMiHOG000166647.
InParanoidiP77165.
KOiK13483.
OMAiCSAIAVI.
OrthoDBiEOG6ZSP3F.
PhylomeDBiP77165.

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNQGEYPED NRVGKHEPHD LSLTRRDLIK VSAATAATAV VYPHSTLAAS
60 70 80 90 100
VPAATPAPEI MPLTLKVNGK TEQLEVDTRT TLLDTLRENL HLIGTKKGCD
110 120 130 140 150
HGQCGACTVL VNGRRLNACL TLAVMHQGAE ITTIEGLGSP DNLHPMQAAF
160 170 180 190 200
IKHDGFQCGY CTSGQICSSV AVLKEIQDGI PSHVTVDLVS APETTADEIR
210 220
ERMSGNICRC GAYANILAAI EDAAGEIKS
Length:229
Mass (Da):24,343
Last modified:February 1, 1997 - v1
Checksum:i216961D7BD21836C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18015.1.
U00096 Genomic DNA. Translation: AAC73389.1.
AP009048 Genomic DNA. Translation: BAE76070.1.
PIRiF64754.
RefSeqiNP_414820.1. NC_000913.3.
WP_000070700.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73389; AAC73389; b0286.
BAE76070; BAE76070; BAE76070.
GeneIDi945330.
KEGGiecj:JW0280.
eco:b0286.
PATRICi32115695. VBIEscCol129921_0290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18015.1.
U00096 Genomic DNA. Translation: AAC73389.1.
AP009048 Genomic DNA. Translation: BAE76070.1.
PIRiF64754.
RefSeqiNP_414820.1. NC_000913.3.
WP_000070700.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77165.
SMRiP77165. Positions 63-225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259786. 3 interactions.
IntActiP77165. 10 interactions.
STRINGi511145.b0286.

Proteomic databases

PaxDbiP77165.
PRIDEiP77165.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73389; AAC73389; b0286.
BAE76070; BAE76070; BAE76070.
GeneIDi945330.
KEGGiecj:JW0280.
eco:b0286.
PATRICi32115695. VBIEscCol129921_0290.

Organism-specific databases

EchoBASEiEB3329.
EcoGeneiEG13559. yagT.

Phylogenomic databases

eggNOGiENOG4107XKX. Bacteria.
COG2080. LUCA.
HOGENOMiHOG000166647.
InParanoidiP77165.
KOiK13483.
OMAiCSAIAVI.
OrthoDBiEOG6ZSP3F.
PhylomeDBiP77165.

Enzyme and pathway databases

BioCyciEcoCyc:G6157-MONOMER.
ECOL316407:JW0280-MONOMER.
MetaCyc:G6157-MONOMER.

Miscellaneous databases

PROiP77165.

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
    Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
    J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPORT VIA THE TAT-SYSTEM.

Entry informationi

Entry nameiYAGT_ECOLI
AccessioniPrimary (citable) accession number: P77165
Secondary accession number(s): Q2MCD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.