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Protein

Putative xanthine dehydrogenase YagT iron-sulfur-binding subunit

Gene

yagT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Iron-sulfur subunit of the xanthine dehydrogenase complex.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi104Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi107Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • electron transfer activity Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • oxidoreductase activity, acting on the aldehyde or oxo group of donors Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism, Purine salvage
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6157-MONOMER
MetaCyc:G6157-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative xanthine dehydrogenase YagT iron-sulfur-binding subunit
Gene namesi
Name:yagT
Ordered Locus Names:b0286, JW0280
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13559 yagT

Subcellular locationi

GO - Cellular componenti

  • periplasmic space Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 53Tat-type signalPROSITE-ProRule annotationAdd BLAST53
ChainiPRO_000018941454 – 229Putative xanthine dehydrogenase YagT iron-sulfur-binding subunitAdd BLAST176

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PaxDbiP77165
PRIDEiP77165

Interactioni

Subunit structurei

Heterotrimer of YagR, YagS and YagT.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
proXP0AFM22EBI-1115563,EBI-1129961

Protein-protein interaction databases

BioGridi4259786, 3 interactors
IntActiP77165, 10 interactors
STRINGi316385.ECDH10B_0274

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi60 – 67Combined sources8
Beta strandi70 – 77Combined sources8
Helixi82 – 88Combined sources7
Beta strandi100 – 104Combined sources5
Beta strandi108 – 111Combined sources4
Beta strandi114 – 117Combined sources4
Helixi118 – 120Combined sources3
Helixi123 – 126Combined sources4
Beta strandi130 – 132Combined sources3
Helixi134 – 137Combined sources4
Helixi145 – 153Combined sources9
Beta strandi157 – 159Combined sources3
Helixi162 – 177Combined sources16
Beta strandi186 – 190Combined sources5
Helixi196 – 202Combined sources7
Turni203 – 205Combined sources3
Helixi213 – 225Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5G5GX-ray1.70A1-229[»]
5G5HX-ray2.30A1-229[»]
ProteinModelPortaliP77165
SMRiP77165
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 1372Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST77

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107XKX Bacteria
COG2080 LUCA
HOGENOMiHOG000166647
InParanoidiP77165
KOiK13483
OMAiENLHPMQ
PhylomeDBiP77165

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR002888 2Fe-2S-bd
IPR036884 2Fe-2S-bd_dom_sf
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR012675 Beta-grasp_dom_sf
IPR006311 TAT_signal
PfamiView protein in Pfam
PF00111 Fer2, 1 hit
PF01799 Fer2_2, 1 hit
SUPFAMiSSF47741 SSF47741, 1 hit
SSF54292 SSF54292, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNQGEYPED NRVGKHEPHD LSLTRRDLIK VSAATAATAV VYPHSTLAAS
60 70 80 90 100
VPAATPAPEI MPLTLKVNGK TEQLEVDTRT TLLDTLRENL HLIGTKKGCD
110 120 130 140 150
HGQCGACTVL VNGRRLNACL TLAVMHQGAE ITTIEGLGSP DNLHPMQAAF
160 170 180 190 200
IKHDGFQCGY CTSGQICSSV AVLKEIQDGI PSHVTVDLVS APETTADEIR
210 220
ERMSGNICRC GAYANILAAI EDAAGEIKS
Length:229
Mass (Da):24,343
Last modified:February 1, 1997 - v1
Checksum:i216961D7BD21836C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA Translation: AAB18015.1
U00096 Genomic DNA Translation: AAC73389.1
AP009048 Genomic DNA Translation: BAE76070.1
PIRiF64754
RefSeqiNP_414820.1, NC_000913.3
WP_000070700.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73389; AAC73389; b0286
BAE76070; BAE76070; BAE76070
GeneIDi945330
KEGGiecj:JW0280
eco:b0286
PATRICifig|1411691.4.peg.1992

Similar proteinsi

Entry informationi

Entry nameiYAGT_ECOLI
AccessioniPrimary (citable) accession number: P77165
Secondary accession number(s): Q2MCD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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