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Protein

2-hydroxy-3-oxopropionate reductase

Gene

glxR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glycerate + NAD(P)+ = 2-hydroxy-3-oxopropanoate + NAD(P)H.

Pathwayi: glycolate degradation

This protein is involved in step 3 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glyoxylate carboligase (gcl)
  3. 2-hydroxy-3-oxopropionate reductase (glxR)
  4. Glycerate kinase (glxK)
This subpathway is part of the pathway glycolate degradation, which is itself part of Organic acid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei94 – 941NADBy similarity
Active sitei169 – 1691By similarity
Binding sitei237 – 2371NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi4 – 1815NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • allantoin assimilation pathway Source: EcoCyc
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • glycolate catabolic process Source: UniProtKB-UniPathway
  • glyoxylate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:G6278-MONOMER.
ECOL316407:JW0497-MONOMER.
MetaCyc:G6278-MONOMER.
UniPathwayiUPA00864; UER00832.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxy-3-oxopropionate reductase (EC:1.1.1.60)
Alternative name(s):
Tartronate semialdehyde reductase
Short name:
TSAR
Gene namesi
Name:glxR
Synonyms:glxB1, ybbQ
Ordered Locus Names:b0509, JW0497
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13265. glxR.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2922922-hydroxy-3-oxopropionate reductasePRO_0000173061Add
BLAST

Proteomic databases

PaxDbiP77161.
PRIDEiP77161.

Expressioni

Inductioni

By glyoxylate.

Interactioni

Protein-protein interaction databases

BioGridi4262821. 13 interactions.
IntActiP77161. 9 interactions.
STRINGi511145.b0509.

Structurei

3D structure databases

ProteinModelPortaliP77161.
SMRiP77161. Positions 1-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CF3. Bacteria.
COG2084. LUCA.
HOGENOMiHOG000219608.
InParanoidiP77161.
KOiK00042.
OMAiGAYLIDM.
OrthoDBiEOG6XDH0X.
PhylomeDBiP77161.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR002204. 3-OH-isobutyrate_DH-rel_CS.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR015815. HIBADH-related.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
IPR006398. Tartro_sem_red.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000103. HIBADH. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01505. tartro_sem_red. 1 hit.
PROSITEiPS00895. 3_HYDROXYISOBUT_DH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLGFIGLGI MGTPMAINLA RAGHQLHVTT IGPVADELLS LGAVSVETAR
60 70 80 90 100
QVTEASDIIF IMVPDTPQVE EVLFGENGCT KASLKGKTIV DMSSISPIET
110 120 130 140 150
KRFARQVNEL GGDYLDAPVS GGEIGAREGT LSIMVGGDEA VFERVKPLFE
160 170 180 190 200
LLGKNITLVG GNGDGQTCKV ANQIIVALNI EAVSEALLFA SKAGADPVRV
210 220 230 240 250
RQALMGGFAS SRILEVHGER MIKRTFNPGF KIALHQKDLN LALQSAKALA
260 270 280 290
LNLPNTATCQ ELFNTCAANG GSQLDHSALV QALELMANHK LA
Length:292
Mass (Da):30,801
Last modified:February 1, 1997 - v1
Checksum:i5D5263231F2910F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89279 Genomic DNA. Translation: AAB93851.1.
U82664 Genomic DNA. Translation: AAB40262.1.
U00096 Genomic DNA. Translation: AAC73611.1.
AP009048 Genomic DNA. Translation: BAE76287.1.
PIRiD64782.
RefSeqiNP_415042.1. NC_000913.3.
WP_000765839.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73611; AAC73611; b0509.
BAE76287; BAE76287; BAE76287.
GeneIDi945146.
KEGGiecj:JW0497.
eco:b0509.
PATRICi32116177. VBIEscCol129921_0530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89279 Genomic DNA. Translation: AAB93851.1.
U82664 Genomic DNA. Translation: AAB40262.1.
U00096 Genomic DNA. Translation: AAC73611.1.
AP009048 Genomic DNA. Translation: BAE76287.1.
PIRiD64782.
RefSeqiNP_415042.1. NC_000913.3.
WP_000765839.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77161.
SMRiP77161. Positions 1-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262821. 13 interactions.
IntActiP77161. 9 interactions.
STRINGi511145.b0509.

Proteomic databases

PaxDbiP77161.
PRIDEiP77161.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73611; AAC73611; b0509.
BAE76287; BAE76287; BAE76287.
GeneIDi945146.
KEGGiecj:JW0497.
eco:b0509.
PATRICi32116177. VBIEscCol129921_0530.

Organism-specific databases

EchoBASEiEB3052.
EcoGeneiEG13265. glxR.

Phylogenomic databases

eggNOGiENOG4105CF3. Bacteria.
COG2084. LUCA.
HOGENOMiHOG000219608.
InParanoidiP77161.
KOiK00042.
OMAiGAYLIDM.
OrthoDBiEOG6XDH0X.
PhylomeDBiP77161.

Enzyme and pathway databases

UniPathwayiUPA00864; UER00832.
BioCyciEcoCyc:G6278-MONOMER.
ECOL316407:JW0497-MONOMER.
MetaCyc:G6278-MONOMER.

Miscellaneous databases

PROiP77161.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR002204. 3-OH-isobutyrate_DH-rel_CS.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR015815. HIBADH-related.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
IPR006398. Tartro_sem_red.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000103. HIBADH. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01505. tartro_sem_red. 1 hit.
PROSITEiPS00895. 3_HYDROXYISOBUT_DH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli."
    Cusa E., Obradors N., Baldoma L., Badia J., Aguilar J.
    J. Bacteriol. 181:7479-7484(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: K12 / ECL1.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiGLXR_ECOLI
AccessioniPrimary (citable) accession number: P77161
Secondary accession number(s): Q2MBR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.