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Protein

Guanine deaminase

Gene

guaD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.

Catalytic activityi

Guanine + H2O = xanthine + NH3.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: guanine degradation

This protein is involved in step 1 of the subpathway that synthesizes xanthine from guanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanine deaminase (guaD)
This subpathway is part of the pathway guanine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes xanthine from guanine, the pathway guanine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi82Zinc; via tele nitrogenBy similarity1
Metal bindingi84Zinc; via tele nitrogenBy similarity1
Metal bindingi237Zinc; via tele nitrogenBy similarity1
Metal bindingi327ZincBy similarity1
Binding sitei327SubstrateBy similarity1

GO - Molecular functioni

  • ammeline aminohydrolase activity Source: EcoCyc
  • guanine deaminase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7502-MONOMER
MetaCyc:G7502-MONOMER
UniPathwayiUPA00603; UER00660

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine deaminase (EC:3.5.4.3)
Short name:
Guanase
Short name:
Guanine aminase
Alternative name(s):
Guanine aminohydrolase
Short name:
GAH
Gene namesi
Name:guaD
Synonyms:ygfP
Ordered Locus Names:b2883, JW5466
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13066 guaD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001222971 – 439Guanine deaminaseAdd BLAST439

Proteomic databases

PaxDbiP76641
PRIDEiP76641

Interactioni

Protein-protein interaction databases

BioGridi4259227, 10 interactors
STRINGi316385.ECDH10B_3058

Structurei

3D structure databases

ProteinModelPortaliP76641
SMRiP76641
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 87Substrate bindingBy similarity4
Regioni209 – 210Substrate bindingBy similarity2
Regioni237 – 240Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107QUY Bacteria
COG0402 LUCA
HOGENOMiHOG000257693
InParanoidiP76641
KOiK01487
OMAiWCFKPCE
PhylomeDBiP76641

Family and domain databases

CDDicd01303 GDEase, 1 hit
Gene3Di2.30.40.10, 2 hits
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR014311 Guanine_deaminase
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
PANTHERiPTHR11271:SF6 PTHR11271:SF6, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
SUPFAMiSSF51338 SSF51338, 1 hit
SSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR02967 guan_deamin, 1 hit

Sequencei

Sequence statusi: Complete.

P76641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSGEHTLKA VRGSFIDVTR TIDNPEEIAS ALRFIEDGLL LIKQGKVEWF
60 70 80 90 100
GEWENGKHQI PDTIRVRDYR GKLIVPGFVD THIHYPQSEM VGAYGEQLLE
110 120 130 140 150
WLNKHTFPTE RRYEDLEYAR EMSAFFIKQL LRNGTTTALV FGTVHPQSVD
160 170 180 190 200
ALFEAASHIN MRMIAGKVMM DRNAPDYLLD TAESSYHQSK ELIERWHKNG
210 220 230 240 250
RLLYAITPRF APTSSPEQMA MAQRLKEEYP DTWVHTHLCE NKDEIAWVKS
260 270 280 290 300
LYPDHDGYLD VYHQYGLTGK NCVFAHCVHL EEKEWDRLSE TKSSIAFCPT
310 320 330 340 350
SNLYLGSGLF NLKKAWQKKV KVGMGTDIGA GTTFNMLQTL NEAYKVLQLQ
360 370 380 390 400
GYRLSAYEAF YLATLGGAKS LGLDDLIGNF LPGKEADFVV MEPTATPLQQ
410 420 430
LRYDNSVSLV DKLFVMMTLG DDRSIYRTYV DGRLVYERN
Length:439
Mass (Da):50,244
Last modified:February 1, 1997 - v1
Checksum:i43389F3AF9E4AD83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA Translation: AAA83064.1
U00096 Genomic DNA Translation: AAC75921.1
AP009048 Genomic DNA Translation: BAE76949.1
PIRiC65072
RefSeqiNP_417359.1, NC_000913.3
WP_001311109.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75921; AAC75921; b2883
BAE76949; BAE76949; BAE76949
GeneIDi947366
KEGGiecj:JW5466
eco:b2883
PATRICifig|511145.12.peg.2976

Similar proteinsi

Entry informationi

Entry nameiGUAD_ECOLI
AccessioniPrimary (citable) accession number: P76641
Secondary accession number(s): Q2M9V7, Q46816
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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