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P76641

- GUAD_ECOLI

UniProt

P76641 - GUAD_ECOLI

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Protein

Guanine deaminase

Gene

guaD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.

Catalytic activityi

Guanine + H2O = xanthine + NH3.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Zinc; via tele nitrogenBy similarity
Metal bindingi84 – 841Zinc; via tele nitrogenBy similarity
Binding sitei87 – 871SubstrateBy similarity
Binding sitei209 – 2091SubstrateBy similarity
Metal bindingi237 – 2371Zinc; via tele nitrogenBy similarity
Binding sitei240 – 2401SubstrateBy similarity
Binding sitei276 – 2761SubstrateBy similarity
Metal bindingi327 – 3271ZincBy similarity

GO - Molecular functioni

  1. ammeline aminohydrolase activity Source: EcoCyc
  2. guanine deaminase activity Source: EcoCyc
  3. zinc ion binding Source: EcoCyc

GO - Biological processi

  1. guanine catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7502-MONOMER.
ECOL316407:JW5466-MONOMER.
MetaCyc:G7502-MONOMER.
RETL1328306-WGS:GSTH-3178-MONOMER.
UniPathwayiUPA00603; UER00660.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine deaminase (EC:3.5.4.3)
Short name:
Guanase
Short name:
Guanine aminase
Alternative name(s):
Guanine aminohydrolase
Short name:
GAH
Gene namesi
Name:guaD
Synonyms:ygfP
Ordered Locus Names:b2883, JW5466
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13066. guaD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Guanine deaminasePRO_0000122297Add
BLAST

Proteomic databases

PaxDbiP76641.
PRIDEiP76641.

Expressioni

Gene expression databases

GenevestigatoriP76641.

Interactioni

Protein-protein interaction databases

STRINGi511145.b2883.

Structurei

3D structure databases

ProteinModelPortaliP76641.
SMRiP76641. Positions 7-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATZ/TRZ family.Curated

Phylogenomic databases

eggNOGiCOG0402.
HOGENOMiHOG000257693.
InParanoidiP76641.
KOiK01487.
OMAiWFGAWED.
OrthoDBiEOG6F55G1.
PhylomeDBiP76641.

Family and domain databases

Gene3Di2.30.40.10. 2 hits.
InterProiIPR006680. Amidohydro_1.
IPR014311. Guanine_deaminase.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PANTHERiPTHR11271:SF6. PTHR11271:SF6. 1 hit.
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 1 hit.
TIGRFAMsiTIGR02967. guan_deamin. 1 hit.

Sequencei

Sequence statusi: Complete.

P76641-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMSGEHTLKA VRGSFIDVTR TIDNPEEIAS ALRFIEDGLL LIKQGKVEWF
60 70 80 90 100
GEWENGKHQI PDTIRVRDYR GKLIVPGFVD THIHYPQSEM VGAYGEQLLE
110 120 130 140 150
WLNKHTFPTE RRYEDLEYAR EMSAFFIKQL LRNGTTTALV FGTVHPQSVD
160 170 180 190 200
ALFEAASHIN MRMIAGKVMM DRNAPDYLLD TAESSYHQSK ELIERWHKNG
210 220 230 240 250
RLLYAITPRF APTSSPEQMA MAQRLKEEYP DTWVHTHLCE NKDEIAWVKS
260 270 280 290 300
LYPDHDGYLD VYHQYGLTGK NCVFAHCVHL EEKEWDRLSE TKSSIAFCPT
310 320 330 340 350
SNLYLGSGLF NLKKAWQKKV KVGMGTDIGA GTTFNMLQTL NEAYKVLQLQ
360 370 380 390 400
GYRLSAYEAF YLATLGGAKS LGLDDLIGNF LPGKEADFVV MEPTATPLQQ
410 420 430
LRYDNSVSLV DKLFVMMTLG DDRSIYRTYV DGRLVYERN
Length:439
Mass (Da):50,244
Last modified:February 1, 1997 - v1
Checksum:i43389F3AF9E4AD83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83064.1.
U00096 Genomic DNA. Translation: AAC75921.1.
AP009048 Genomic DNA. Translation: BAE76949.1.
PIRiC65072.
RefSeqiNP_417359.1. NC_000913.3.
YP_491085.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75921; AAC75921; b2883.
BAE76949; BAE76949; BAE76949.
GeneIDi12930233.
947366.
KEGGiecj:Y75_p2816.
eco:b2883.
PATRICi32121176. VBIEscCol129921_2976.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83064.1 .
U00096 Genomic DNA. Translation: AAC75921.1 .
AP009048 Genomic DNA. Translation: BAE76949.1 .
PIRi C65072.
RefSeqi NP_417359.1. NC_000913.3.
YP_491085.1. NC_007779.1.

3D structure databases

ProteinModelPortali P76641.
SMRi P76641. Positions 7-439.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b2883.

Proteomic databases

PaxDbi P76641.
PRIDEi P76641.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75921 ; AAC75921 ; b2883 .
BAE76949 ; BAE76949 ; BAE76949 .
GeneIDi 12930233.
947366.
KEGGi ecj:Y75_p2816.
eco:b2883.
PATRICi 32121176. VBIEscCol129921_2976.

Organism-specific databases

EchoBASEi EB2878.
EcoGenei EG13066. guaD.

Phylogenomic databases

eggNOGi COG0402.
HOGENOMi HOG000257693.
InParanoidi P76641.
KOi K01487.
OMAi WFGAWED.
OrthoDBi EOG6F55G1.
PhylomeDBi P76641.

Enzyme and pathway databases

UniPathwayi UPA00603 ; UER00660 .
BioCyci EcoCyc:G7502-MONOMER.
ECOL316407:JW5466-MONOMER.
MetaCyc:G7502-MONOMER.
RETL1328306-WGS:GSTH-3178-MONOMER.

Miscellaneous databases

PROi P76641.

Gene expression databases

Genevestigatori P76641.

Family and domain databases

Gene3Di 2.30.40.10. 2 hits.
InterProi IPR006680. Amidohydro_1.
IPR014311. Guanine_deaminase.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view ]
PANTHERi PTHR11271:SF6. PTHR11271:SF6. 1 hit.
Pfami PF01979. Amidohydro_1. 1 hit.
[Graphical view ]
SUPFAMi SSF51338. SSF51338. 1 hit.
TIGRFAMsi TIGR02967. guan_deamin. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Identification, expression, and characterization of Escherichia coli guanine deaminase."
    Maynes J.T., Yuan R.G., Snyder F.F.
    J. Bacteriol. 182:4658-4660(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiGUAD_ECOLI
AccessioniPrimary (citable) accession number: P76641
Secondary accession number(s): Q2M9V7, Q46816
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3