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P76641 (GUAD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine deaminase

Short name=Guanase
Short name=Guanine aminase
EC=3.5.4.3
Alternative name(s):
Guanine aminohydrolase
Short name=GAH
Gene names
Name:guaD
Synonyms:ygfP
Ordered Locus Names:b2883, JW5466
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.

Catalytic activity

Guanine + H2O = xanthine + NH3.

Cofactor

Binds 1 zinc ion per subunit.

Pathway

Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.

Sequence similarities

Belongs to the ATZ/TRZ family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 439439Guanine deaminase
PRO_0000122297

Sites

Metal binding821Zinc; via tele nitrogen By similarity
Metal binding841Zinc; via tele nitrogen By similarity
Metal binding2371Zinc; via tele nitrogen By similarity
Metal binding3271Zinc By similarity
Binding site871Substrate By similarity
Binding site2091Substrate By similarity
Binding site2401Substrate By similarity
Binding site2761Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P76641 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 43389F3AF9E4AD83

FASTA43950,244
        10         20         30         40         50         60 
MMSGEHTLKA VRGSFIDVTR TIDNPEEIAS ALRFIEDGLL LIKQGKVEWF GEWENGKHQI 

        70         80         90        100        110        120 
PDTIRVRDYR GKLIVPGFVD THIHYPQSEM VGAYGEQLLE WLNKHTFPTE RRYEDLEYAR 

       130        140        150        160        170        180 
EMSAFFIKQL LRNGTTTALV FGTVHPQSVD ALFEAASHIN MRMIAGKVMM DRNAPDYLLD 

       190        200        210        220        230        240 
TAESSYHQSK ELIERWHKNG RLLYAITPRF APTSSPEQMA MAQRLKEEYP DTWVHTHLCE 

       250        260        270        280        290        300 
NKDEIAWVKS LYPDHDGYLD VYHQYGLTGK NCVFAHCVHL EEKEWDRLSE TKSSIAFCPT 

       310        320        330        340        350        360 
SNLYLGSGLF NLKKAWQKKV KVGMGTDIGA GTTFNMLQTL NEAYKVLQLQ GYRLSAYEAF 

       370        380        390        400        410        420 
YLATLGGAKS LGLDDLIGNF LPGKEADFVV MEPTATPLQQ LRYDNSVSLV DKLFVMMTLG 

       430 
DDRSIYRTYV DGRLVYERN 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Identification, expression, and characterization of Escherichia coli guanine deaminase."
Maynes J.T., Yuan R.G., Snyder F.F.
J. Bacteriol. 182:4658-4660(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U28375 Genomic DNA. Translation: AAA83064.1.
U00096 Genomic DNA. Translation: AAC75921.1.
AP009048 Genomic DNA. Translation: BAE76949.1.
PIRC65072.
RefSeqNP_417359.1. NC_000913.3.
YP_491085.1. NC_007779.1.

3D structure databases

ProteinModelPortalP76641.
SMRP76641. Positions 7-439.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING511145.b2883.

Proteomic databases

PaxDbP76641.
PRIDEP76641.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75921; AAC75921; b2883.
BAE76949; BAE76949; BAE76949.
GeneID12930233.
947366.
KEGGecj:Y75_p2816.
eco:b2883.
PATRIC32121176. VBIEscCol129921_2976.

Organism-specific databases

EchoBASEEB2878.
EcoGeneEG13066. guaD.

Phylogenomic databases

eggNOGCOG0402.
HOGENOMHOG000257693.
KOK01487.
OMAWFGAWED.
OrthoDBEOG6F55G1.
PhylomeDBP76641.

Enzyme and pathway databases

BioCycEcoCyc:G7502-MONOMER.
ECOL316407:JW5466-MONOMER.
MetaCyc:G7502-MONOMER.
RETL1328306-WGS:GSTH-3178-MONOMER.
UniPathwayUPA00603; UER00660.

Gene expression databases

GenevestigatorP76641.

Family and domain databases

Gene3D2.30.40.10. 2 hits.
InterProIPR006680. Amidohydro_1.
IPR014311. Guanine_deaminase.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PANTHERPTHR11271:SF6. PTHR11271:SF6. 1 hit.
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMSSF51338. SSF51338. 1 hit.
TIGRFAMsTIGR02967. guan_deamin. 1 hit.
ProtoNetSearch...

Other

PROP76641.

Entry information

Entry nameGUAD_ECOLI
AccessionPrimary (citable) accession number: P76641
Secondary accession number(s): Q2M9V7, Q46816
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene