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P76641

- GUAD_ECOLI

UniProt

P76641 - GUAD_ECOLI

Protein

Guanine deaminase

Gene

guaD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.

    Catalytic activityi

    Guanine + H2O = xanthine + NH3.

    Cofactori

    Binds 1 zinc ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi82 – 821Zinc; via tele nitrogenBy similarity
    Metal bindingi84 – 841Zinc; via tele nitrogenBy similarity
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei209 – 2091SubstrateBy similarity
    Metal bindingi237 – 2371Zinc; via tele nitrogenBy similarity
    Binding sitei240 – 2401SubstrateBy similarity
    Binding sitei276 – 2761SubstrateBy similarity
    Metal bindingi327 – 3271ZincBy similarity

    GO - Molecular functioni

    1. ammeline aminohydrolase activity Source: EcoCyc
    2. guanine deaminase activity Source: EcoCyc
    3. zinc ion binding Source: EcoCyc

    GO - Biological processi

    1. guanine catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:G7502-MONOMER.
    ECOL316407:JW5466-MONOMER.
    MetaCyc:G7502-MONOMER.
    RETL1328306-WGS:GSTH-3178-MONOMER.
    UniPathwayiUPA00603; UER00660.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine deaminase (EC:3.5.4.3)
    Short name:
    Guanase
    Short name:
    Guanine aminase
    Alternative name(s):
    Guanine aminohydrolase
    Short name:
    GAH
    Gene namesi
    Name:guaD
    Synonyms:ygfP
    Ordered Locus Names:b2883, JW5466
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13066. guaD.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 439439Guanine deaminasePRO_0000122297Add
    BLAST

    Proteomic databases

    PaxDbiP76641.
    PRIDEiP76641.

    Expressioni

    Gene expression databases

    GenevestigatoriP76641.

    Interactioni

    Protein-protein interaction databases

    STRINGi511145.b2883.

    Structurei

    3D structure databases

    ProteinModelPortaliP76641.
    SMRiP76641. Positions 7-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATZ/TRZ family.Curated

    Phylogenomic databases

    eggNOGiCOG0402.
    HOGENOMiHOG000257693.
    KOiK01487.
    OMAiWFGAWED.
    OrthoDBiEOG6F55G1.
    PhylomeDBiP76641.

    Family and domain databases

    Gene3Di2.30.40.10. 2 hits.
    InterProiIPR006680. Amidohydro_1.
    IPR014311. Guanine_deaminase.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view]
    PANTHERiPTHR11271:SF6. PTHR11271:SF6. 1 hit.
    PfamiPF01979. Amidohydro_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF51338. SSF51338. 1 hit.
    TIGRFAMsiTIGR02967. guan_deamin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P76641-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMSGEHTLKA VRGSFIDVTR TIDNPEEIAS ALRFIEDGLL LIKQGKVEWF    50
    GEWENGKHQI PDTIRVRDYR GKLIVPGFVD THIHYPQSEM VGAYGEQLLE 100
    WLNKHTFPTE RRYEDLEYAR EMSAFFIKQL LRNGTTTALV FGTVHPQSVD 150
    ALFEAASHIN MRMIAGKVMM DRNAPDYLLD TAESSYHQSK ELIERWHKNG 200
    RLLYAITPRF APTSSPEQMA MAQRLKEEYP DTWVHTHLCE NKDEIAWVKS 250
    LYPDHDGYLD VYHQYGLTGK NCVFAHCVHL EEKEWDRLSE TKSSIAFCPT 300
    SNLYLGSGLF NLKKAWQKKV KVGMGTDIGA GTTFNMLQTL NEAYKVLQLQ 350
    GYRLSAYEAF YLATLGGAKS LGLDDLIGNF LPGKEADFVV MEPTATPLQQ 400
    LRYDNSVSLV DKLFVMMTLG DDRSIYRTYV DGRLVYERN 439
    Length:439
    Mass (Da):50,244
    Last modified:February 1, 1997 - v1
    Checksum:i43389F3AF9E4AD83
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28375 Genomic DNA. Translation: AAA83064.1.
    U00096 Genomic DNA. Translation: AAC75921.1.
    AP009048 Genomic DNA. Translation: BAE76949.1.
    PIRiC65072.
    RefSeqiNP_417359.1. NC_000913.3.
    YP_491085.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75921; AAC75921; b2883.
    BAE76949; BAE76949; BAE76949.
    GeneIDi12930233.
    947366.
    KEGGiecj:Y75_p2816.
    eco:b2883.
    PATRICi32121176. VBIEscCol129921_2976.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28375 Genomic DNA. Translation: AAA83064.1 .
    U00096 Genomic DNA. Translation: AAC75921.1 .
    AP009048 Genomic DNA. Translation: BAE76949.1 .
    PIRi C65072.
    RefSeqi NP_417359.1. NC_000913.3.
    YP_491085.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P76641.
    SMRi P76641. Positions 7-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b2883.

    Proteomic databases

    PaxDbi P76641.
    PRIDEi P76641.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75921 ; AAC75921 ; b2883 .
    BAE76949 ; BAE76949 ; BAE76949 .
    GeneIDi 12930233.
    947366.
    KEGGi ecj:Y75_p2816.
    eco:b2883.
    PATRICi 32121176. VBIEscCol129921_2976.

    Organism-specific databases

    EchoBASEi EB2878.
    EcoGenei EG13066. guaD.

    Phylogenomic databases

    eggNOGi COG0402.
    HOGENOMi HOG000257693.
    KOi K01487.
    OMAi WFGAWED.
    OrthoDBi EOG6F55G1.
    PhylomeDBi P76641.

    Enzyme and pathway databases

    UniPathwayi UPA00603 ; UER00660 .
    BioCyci EcoCyc:G7502-MONOMER.
    ECOL316407:JW5466-MONOMER.
    MetaCyc:G7502-MONOMER.
    RETL1328306-WGS:GSTH-3178-MONOMER.

    Miscellaneous databases

    PROi P76641.

    Gene expression databases

    Genevestigatori P76641.

    Family and domain databases

    Gene3Di 2.30.40.10. 2 hits.
    InterProi IPR006680. Amidohydro_1.
    IPR014311. Guanine_deaminase.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view ]
    PANTHERi PTHR11271:SF6. PTHR11271:SF6. 1 hit.
    Pfami PF01979. Amidohydro_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51338. SSF51338. 1 hit.
    TIGRFAMsi TIGR02967. guan_deamin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Identification, expression, and characterization of Escherichia coli guanine deaminase."
      Maynes J.T., Yuan R.G., Snyder F.F.
      J. Bacteriol. 182:4658-4660(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiGUAD_ECOLI
    AccessioniPrimary (citable) accession number: P76641
    Secondary accession number(s): Q2M9V7, Q46816
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3